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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIPuro-HAmSuz12 (#RDB14116)

Expression vector of HA tagged mouse Suz12.

Alternative name clone 6
Clone info. Expression vector of HA tagged mouse Suz12.
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Suz12 cDNA
Depositor|Developer Okazaki, Yasuji | Iseki, Hiroyoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
MTAに書く付加的使用条件：
利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14116 pCAGIPuro-HAmSuz12 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIPuro-HAmSuz12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14116).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14116_A6Dcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14116_A6Dca.seq check
>D03255K8_A6Dc_pAxCA_F1_H02_23_ABI24.ab1
    1 CTTACGCAAT CTGCTACATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
   61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGACCACC ATGTACCCTT
  121 ACGACGTGCC TGACTACGCC GGCGGCGGAA TGGCGCCTCA GAAGCACGGC GGTGGGGGAG
  181 GGGGCGGCTC GGGGCCCAGC GCGGGGTCCG GGGGAGGCGG CTTCGGGGGT TCGGCGGCGG
  241 CGGTGGCGGC GGCGGCTTCG GGCGGCAAAT CCGGCGGCGG GGGCTGTGGA GGCGGCGGCA
  301 GTTACTCGGC CTCCTCCTCC TCGGCGGCGG CCGCGGCGGC GGCGGCGGGG GCCGCGGTGT
  361 TGCCGGTGAA GAAGCCGAAA ATGGAGCACG TCCAGGCTGA CCACGAGCTT TTCCTCCAGG
  421 CCTTTGAGAA ACCAACACAG ATATATAGAT TTCTTCGAAC TCGAAATCTT ATCGCACCAA
  481 TATTTTTGCA CAGAACTCTT ACTTACATGT CTCATCGAAA TTCCAGAACA AGCATCAAAA
  541 GGAAAACATT TAAAGTTGAT GATATGTTAT CAAAAGTAGA GAAAATGAAA GGAGAGCAAG
  601 AATCTCATAG CTTGTCTGCA CATTTGCAAC TTACATTTAC CGGTTTCTTC CACAAAAATG
  661 ATAAGCCATC ACAAAACTCA GAAAATGAAC AAAATTCTGT TACCCTGGAA GTCCTGCTTG
  721 TGAAAGTTTG CCACAAAAAA AGGAAGGATG TAAGTTGTCC AATAAGACAA GTCCCTACTG
  781 GTAAAAAGCA GGTGCCTTTG AATCCTGACC TCAATCAAAC AAAACCAGGA AATTTTCCAT
  841 CCCTGGCAGT TTCCAGTAAT GAATTTGAAC CTAGTAACAG CCATATGGTG AAGTCCTACT
  901 CGTTGCTGTT TAGAGTAACT CGTCCAGGGA GAAGAGAATT TAAATGGAAT GATTAATGGA
  961 GAAACCCAAT GAAATATTGA TGTCAGTGAG ACCTTTCCAA GCCCAGAGAA AACGAAATTC
 1021 GCGAGATGGA GGAAAAGACA TTTTGTGGCA CAATTGACCA GGTTTTTGAC TACACAGCGA
1081 TTCACAGCCT TTTAGAATTG GGGCCA
//
Primer: IRES-R
Sequence file: RDB14116_A6Dcb.seq check
>D03255K8_A6Dc_IRES-R_F09_18_ABI24.ab1
    1 GGCAAGCGGG CTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGCGGAATT
   61 CGATATCAAG CTTATCGAGC GGCCGCTCGA TACCGTCGAT CTCAGTGGTA TTTGTGAGCC
  121 AGTTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTGAATTCCT
  181 CGACTCAGAG TTTTTGTTTC TTGCTCTGTT TTGGAACCCC TGAGACACCG TCAGTTTCCA
  241 AAGCTTTCTC TTTTGAGTTA GTTTCACTGA ATCCATTTGC TGTTCCATTT TGTTCCTCAG
  301 CTATTTCTTC ATTTGAAGGG GTCGCAGATT CTCCTTTTTC TAGTTTTTGC TGCATTTCTC
  361 GGAGCTTGGT AACAGCTTTA TCTATTGACA TTATGCTAAT AAGATTAAAG TCATGCATGC
  421 TGACTAGATG AAGCATGAAG TTTCGACATA AATTCTTCTT AATTATTTTC TGTCCATAAT
  481 TTTCTACAAA CAGCATACAG GCATGATTCA TTTGATTGTC AGCAATAAAT CCATGCTTCA
  541 TGACATGGAG GTTCCACAGC TTCATCACTT CTTTCTCTCC TTCATTCACA TCAGAAAAAT
  601 TCTTCAATTT GAGTAATGGT TTTTTCTCTC AGCCATTCTG GATCTTTCTC ATCTTCACTA
  661 TCTACTTCCA TTTCTTGTGG CCGAAGAGGT AAGCAGTATC ACTGTGGAAA TAGAGACGAT
  721 TGTGGCCACT GCTGTATGTT CTCTGCTGCT CCACTTCTCC ATCTTTCAGA TTCAAGAAAC
  781 TCCGACATGC TTGCTTTTGT TCTTTTTGGC CCTGCAAACA AGAATATGTG TGATAGGTGT
  841 TCTCTTTACC GGGTCCAATT CGACTAAAGC AAATCCCAGG TTTGGCGATG TATTATCCTG
  901 AGATTCCTGC CATAGGAAGC ATCATAACAC CTCATTGATG GAAACATCTA TCTAGCACTT
961 TTGGATGAAT ATACGTAATT GAATGACCTG CTGTGCAAGC CTTTTCAGAA TGCTG
//
Primer: IRES-F
Sequence file: RDB14116_A6Dcc.seq check
>D03255K8_A6Dc_IRES-F_A03_03_ABI24.ab1
    1 GTAAAGCTTT ATGTGTTTAG TCGAGGTTAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGTGTC ATTCTATTCT GGGGGGGTGG GGTGGGGCAG GACAGCAAAG
  961 GGGGAGGATT TGGGAAGACA ATAGCAGGCA TGCTGGGGGA TGCGGTGGCT CTATGGCTTT
 1021 CTGAGCGGAA AGAACCCTGC AGCCCAAGCC TGCGTAATCA TGGTTCAATA GCTGATACCT
1081 GTGTGTGAAA TTGTTATCCG CCTCACCAAT TCCACACACA GAC
//
Primer: BGHpA_F
Sequence file: RDB14116_A6Dcd.seq check
>D03255K8_A6Dc_BGHpA_F_A06_03_ABI24.ab1
    1 TCGTAAGGTT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
   61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA
  121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
  181 CCGCTCACAA TTCCACACAA CATACGAGCC GAAAGCATAA AGTGTAAAGC CTGGGGTGCC
  241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
  301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
  361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
  421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
  481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
  541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
  661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
  721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
  781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
  841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
  901 CATAGGCTCC GCCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
  961 AAACCCGACA GGACTATAAA GATACCCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
 1021 CTTCTGTTCG ACCCTGCGCT TTACCGGATA CTGTCGCGTT TTCTCCGTTC CGGTAGCCGT
1081 TGCGCTTTCT CATAGCTCCA CCGCTGATAG GATAATTCTC A
//
Primer: Amp_R
Sequence file: RDB14116_A6Dce.seq check
>D03255K8_A6Dc_Amp_R_A10_01_ABI24.ab1
    1 CTCGTTTTCA TGACGCTTTG GAAACAAAGA CTGTATTTCC TGGAAATTAA TGTTTATTCA
   61 ATAAACTGTG TATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG
  121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC
  181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA
  241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG
  301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA
  361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG
  421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT
  481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC
  541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC
  601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG
  721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT
  781 AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG
  841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
  901 CGCCCAATAG GACTTTCCAT TTGACGTCAA TGGGGTGGGA GTATTACGTA AACTGCCCAC
  961 TTGGCAGTTA CATCCAGTGT ATCATAATGC TAGTTACGCC CCCTATGACG GTCATGAACG
1021 GTAATGACCG GCCTGGCATA TGCCAGTACA TTGACTTATT GGGGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]