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pCAGIPuro-HAmcMyc_DN143

Expression vector of HA tagged mouse cMyc, deletion mutant.

Catalog number RDB14101
Resource name pCAGIPuro-HAmcMyc_DN143
Clone info. Expression vector of HA tagged mouse cMyc, deletion of N143 (a.a from the 1st to the 143rd of the N-terminal).
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Myc cDNA
Depositor|Developer Okazaki, Yasushi | Iseki, Hiroyoshi |
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External Database
mouse Myc

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
          Additional terms and conditions for distribution CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
          付加的提供条件 利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14101 pCAGIPuro-HAmcMyc_DN143 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14101_A6Dcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB14101_A6Dca.seq check
          >D03255J2_A6Dc_pAxCA_F1_B07_04_ABI24.ab1
              1 GGTACGGTCC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
             61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGTAGGC CACCATGTAC
            121 CCATACGACG TCCCAGACTA CGCTAAGCTG GTCTCGGAGA AGCTGGCCTC CTACCAGGCT
            181 GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG CTCCACCTCC
            241 AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC CTCAGTGGTC
            301 TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC CGATTCCACG
            361 GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG GGCCAGCCCT
            421 GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC TGAAGAAGAG
            481 CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG
            541 AGGTCGGAGT CGGGCTCATC TCCATCCCGA GGCCACAGCA AACCTCCGCA CAGCCCACTG
            601 GTCCTCAAGA GGTGCCACGT CTCCACTCAC CAGCACAACT ACGCCGCACC CCCCTCCACA
            661 AGAAGGACTA TCCAGCTGCC AAGAGGGCCA AGTTGGACAG TGGCAGGTCC TGAAGCAGAT
            721 CAGCAACAAC CGCAAGTGCT CAGCCCCCAG TCCTCAGACA CGGAGGAAAA CGACAGAGGC
            781 GGACACACAA CGTCTTGGAA CGTCAGAGAG ACGAGCTGAG CGCAGCTTTT TGCCCTGGCG
            841 GTGACAGAAT CCTGATGGAA ACAACGAAAG CTCCGAGGTA GTGATCCTCA AAAGGCCACC
            901 GCTTACATTC TGTTCCATTC AGCGAGACGA GATAAAGCTC CACCTTGCAG ACTTATTGGA
          961 CGCAGGACAT GGAACTAATC GACGCTGAGC TTGTCTTAAG TAAAG
          //
          Primer: Myc-M_F
          Sequence file: RDB14101_A6Dcb.seq check
          >D03255J2_A6Dc_EcoRI_Myc-M_F_A04_01_ABI08.ab1
              1 ACTGGCAATC CTGTACCTCG TCCGATTCCA CGGCCTTCTC TCCTTCCTCG GACTCGCTGC
             61 TGTCCTCCGA GTCCTCCCCA CGGGCCAGCC CTGAGCCCCT AGTGCTGCAT GAGGAGACAC
            121 CGCCCACCAC CAGCAGCGAC TCTGAAGAAG AGCAAGAAGA TGAGGAAGAA ATTGATGTGG
            181 TGTCTGTGGA GAAGAGGCAA ACCCCTGCCA AGAGGTCGGA GTCGGGCTCA TCTCCATCCC
            241 GAGGCCACAG CAAACCTCCG CACAGCCCAC TGGTCCTCAA GAGGTGCCAC GTCTCCACTC
            301 ACCAGCACAA CTACGCCGCA CCCCCCTCCA CAAGGAAGGA CTATCCAGCT GCCAAGAGGG
            361 CCAAGTTGGA CAGTGGCAGG GTCCTGAAGC AGATCAGCAA CAACCGCAAG TGCTCCAGCC
            421 CCAGGTCCTC AGACACGGAG GAAAACGACA AGAGGCGGAC ACACAACGTC TTGGAACGTC
            481 AGAGGAGGAA CGAGCTGAAG CGCAGCTTTT TTGCCCTGCG TGACCAGATC CCTGAATTGG
            541 AAAACAACGA AAAGGCCCCC AAGGTAGTGA TCCTCAAAAA AGCCACCGCC TACATCCTGT
            601 CCATTCAAGC AGACGAGCAC AAGCTCACCT CTGAAAAGGA CTTATTGAGG AAACGACGAG
            661 AACAATTGAA ACACAAACTC GAACAGCTTC GAAACTCTGG TGCATAAGGT GAAGGGGGCG
          721 GCCGCTCGAT AAGCTTGATA ATCGAATTGA
          //
          Primer: IRES-F
          Sequence file: RDB14101_A6Dcc.seq check
          >D03255J2_A6Dc_IRES-F_D07_10_ABI24.ab1
              1 GGCAATCTTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
             61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC
            121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG
            181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG
            241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
            301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
            361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
            421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
            481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
            541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
            601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
            661 AAGGACCGCG CCACCTGGTG CATGACCCCG CAAGCCCGGT GCCTGACGCC CGCCCCCACG
            721 ACCCGCAGCG CCCGACCGAA GGAGCGCCAC GACCCATGCA TCGATGATCT AGAGCTCCGC
            781 TGATCAGCCT CGACTGGTGC CTTCTAGTGC AGCATCTGTT GTTTGCCCCT CCCCCGTGCT
            841 CTGGACCTTG AGGTGCCACT CCCACTGTCG TTTCTAATAA ATGAAGAATT GCCACTCGCA
            901 ATTGTCTGGA TAGGTCCATT CTATTCGGGG TGAGTTGCAG ACCCCAGGGA ATGAACAACT
          961 CAGCATGCTG ATATTCGAGT GTG
          //
          Primer: BGHpA_F
          Sequence file: RDB14101_A6Dcd.seq check
          >D03255J2_A6Dc_BGHpA_F_E07_13_ABI24.ab1
              1 TCGTAATGGT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
             61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA
            121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
            181 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
            241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
            301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
            361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
            421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
            481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
            541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
            601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
            661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
            721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
            781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
            841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
            901 CATAGGCTCC GCCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
            961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
           1021 TCCTGTTCCG ACCCTGCCGC TTACGGGATA CCCTGTCCGC CTTTCTCCCG TCCGGAGCGT
           1081 GCGCTTTCTC ATAGCTCACG CTGTAAGTAT CTCAGTCGAT GTAAGGTCGG TTCGTTCAGC
          1141 TGACATGATG ATGACACGCG GA
          //
          Primer: Amp_R
          Sequence file: RDB14101_A6Dce.seq check
          >D03255J2_A6Dc_Amp_R_F07_16_ABI24.ab1
              1 CTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
             61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
            121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
            181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
            241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
            301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
            361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
            421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
            481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
            541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
            601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
            661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
            721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
            781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
            841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
            901 CGCCCAATAG GGACTTTCCC ATTGACGTCA ATGGTGGAGT ATTTACGGTA AACTGCCCAC
            961 TTGGCAGTAC ATCAGTGTAT CATATGCCAA GTACGCCCCC TATGACGTCA TGACGTAATT
           1021 GACCGCTGCC ATATGCCAGT ACATGACGTA ATGGAACCTT ACTACTGGCA GTACCTTACG
          1081 TATAGTTCAA TCGCCTATTA C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl