Resource data sheet
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pCAGIPuro-HAmcMyc_DN143

Expression vector of HA tagged mouse cMyc, deletion mutant.

Catalog number RDB14101
Resource name pCAGIPuro-HAmcMyc_DN143
Clone info. Expression vector of HA tagged mouse cMyc, deletion of N143 (a.a from the 1st to the 143rd of the N-terminal).
Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Myc cDNA
Depositor Okazaki, Yasushi | Iseki, Hiroyoshi |
 
Other clones in our bank mouse Myc (NCBI Gene 17869) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions for distribution CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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RDB14101 pCAGIPuro-HAmcMyc_DN143 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14101_A6Dcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14101_A6Dca.seq
>D03255J2_A6Dc_pAxCA_F1_B07_04_ABI24.ab1
    1 GGTACGGTCC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG
   61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGTAGGC CACCATGTAC
  121 CCATACGACG TCCCAGACTA CGCTAAGCTG GTCTCGGAGA AGCTGGCCTC CTACCAGGCT
  181 GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG CTCCACCTCC
  241 AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC CTCAGTGGTC
  301 TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC CGATTCCACG
  361 GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG GGCCAGCCCT
  421 GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC TGAAGAAGAG
  481 CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG
  541 AGGTCGGAGT CGGGCTCATC TCCATCCCGA GGCCACAGCA AACCTCCGCA CAGCCCACTG
  601 GTCCTCAAGA GGTGCCACGT CTCCACTCAC CAGCACAACT ACGCCGCACC CCCCTCCACA
  661 AGAAGGACTA TCCAGCTGCC AAGAGGGCCA AGTTGGACAG TGGCAGGTCC TGAAGCAGAT
  721 CAGCAACAAC CGCAAGTGCT CAGCCCCCAG TCCTCAGACA CGGAGGAAAA CGACAGAGGC
  781 GGACACACAA CGTCTTGGAA CGTCAGAGAG ACGAGCTGAG CGCAGCTTTT TGCCCTGGCG
  841 GTGACAGAAT CCTGATGGAA ACAACGAAAG CTCCGAGGTA GTGATCCTCA AAAGGCCACC
  901 GCTTACATTC TGTTCCATTC AGCGAGACGA GATAAAGCTC CACCTTGCAG ACTTATTGGA
961 CGCAGGACAT GGAACTAATC GACGCTGAGC TTGTCTTAAG TAAAG
//
Primer: Myc-M_F RDB14101_A6Dcb.seq
>D03255J2_A6Dc_EcoRI_Myc-M_F_A04_01_ABI08.ab1
    1 ACTGGCAATC CTGTACCTCG TCCGATTCCA CGGCCTTCTC TCCTTCCTCG GACTCGCTGC
   61 TGTCCTCCGA GTCCTCCCCA CGGGCCAGCC CTGAGCCCCT AGTGCTGCAT GAGGAGACAC
  121 CGCCCACCAC CAGCAGCGAC TCTGAAGAAG AGCAAGAAGA TGAGGAAGAA ATTGATGTGG
  181 TGTCTGTGGA GAAGAGGCAA ACCCCTGCCA AGAGGTCGGA GTCGGGCTCA TCTCCATCCC
  241 GAGGCCACAG CAAACCTCCG CACAGCCCAC TGGTCCTCAA GAGGTGCCAC GTCTCCACTC
  301 ACCAGCACAA CTACGCCGCA CCCCCCTCCA CAAGGAAGGA CTATCCAGCT GCCAAGAGGG
  361 CCAAGTTGGA CAGTGGCAGG GTCCTGAAGC AGATCAGCAA CAACCGCAAG TGCTCCAGCC
  421 CCAGGTCCTC AGACACGGAG GAAAACGACA AGAGGCGGAC ACACAACGTC TTGGAACGTC
  481 AGAGGAGGAA CGAGCTGAAG CGCAGCTTTT TTGCCCTGCG TGACCAGATC CCTGAATTGG
  541 AAAACAACGA AAAGGCCCCC AAGGTAGTGA TCCTCAAAAA AGCCACCGCC TACATCCTGT
  601 CCATTCAAGC AGACGAGCAC AAGCTCACCT CTGAAAAGGA CTTATTGAGG AAACGACGAG
  661 AACAATTGAA ACACAAACTC GAACAGCTTC GAAACTCTGG TGCATAAGGT GAAGGGGGCG
721 GCCGCTCGAT AAGCTTGATA ATCGAATTGA
//
Primer: IRES-F RDB14101_A6Dcc.seq
>D03255J2_A6Dc_IRES-F_D07_10_ABI24.ab1
    1 GGCAATCTTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC
  121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG
  181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG
  241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
  301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
  361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
  421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
  481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
  541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
  601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
  661 AAGGACCGCG CCACCTGGTG CATGACCCCG CAAGCCCGGT GCCTGACGCC CGCCCCCACG
  721 ACCCGCAGCG CCCGACCGAA GGAGCGCCAC GACCCATGCA TCGATGATCT AGAGCTCCGC
  781 TGATCAGCCT CGACTGGTGC CTTCTAGTGC AGCATCTGTT GTTTGCCCCT CCCCCGTGCT
  841 CTGGACCTTG AGGTGCCACT CCCACTGTCG TTTCTAATAA ATGAAGAATT GCCACTCGCA
  901 ATTGTCTGGA TAGGTCCATT CTATTCGGGG TGAGTTGCAG ACCCCAGGGA ATGAACAACT
961 CAGCATGCTG ATATTCGAGT GTG
//
Primer: BGHpA_F RDB14101_A6Dcd.seq
>D03255J2_A6Dc_BGHpA_F_E07_13_ABI24.ab1
    1 TCGTAATGGT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
   61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA
  121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
  181 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
  241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
  301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
  361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
  421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
  481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC
  541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG
  661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
  721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
  781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
  841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
  901 CATAGGCTCC GCCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
  961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
 1021 TCCTGTTCCG ACCCTGCCGC TTACGGGATA CCCTGTCCGC CTTTCTCCCG TCCGGAGCGT
 1081 GCGCTTTCTC ATAGCTCACG CTGTAAGTAT CTCAGTCGAT GTAAGGTCGG TTCGTTCAGC
1141 TGACATGATG ATGACACGCG GA
//
Primer: Amp_R RDB14101_A6Dce.seq
>D03255J2_A6Dc_Amp_R_F07_16_ABI24.ab1
    1 CTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
   61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
  121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
  181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
  241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
  301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
  361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
  421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
  481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
  541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
  601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
  721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
  781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
  841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
  901 CGCCCAATAG GGACTTTCCC ATTGACGTCA ATGGTGGAGT ATTTACGGTA AACTGCCCAC
  961 TTGGCAGTAC ATCAGTGTAT CATATGCCAA GTACGCCCCC TATGACGTCA TGACGTAATT
 1021 GACCGCTGCC ATATGCCAGT ACATGACGTA ATGGAACCTT ACTACTGGCA GTACCTTACG
1081 TATAGTTCAA TCGCCTATTA C
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl