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pMXs-mEed (#RDB14059)

Retroviral expression vector of mouse Eed, SNP285A/T

Alternative name clone 67-9
Clone info. Retroviral expression vector of mouse Eed, SNP285A/T
Comment Expression was confirmed by the depositor.
Vector backbone pMXs Retroviral Vector (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name mouse Eed cDNA
Depositor|Developer Okazaki, Yasuji | Iseki, Hiroyoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14059 pMXs-mEed DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-mEed was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14059).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14059_A6Dcp1_v2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2
Sequence file: RDB14059_A6Dca.seq check
>D03255E5_A6Dc_2_MMLV-pol_F2_E12_15.ab1
    1 TTGGGGGGGG GTGGACATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCACC
   61 ATGTCCGAGA GGGAAGTGTC GACTGCGCCG GCGGGAACAG ACATGCCCGC GGCCAAGAAG
  121 CAGAAGTTGA GCAGCGACGA GAACAGCAAC CCGGACCTCT CGGGAGACGA AAATGACGAT
  181 GCTGTCAGTA TTGAGAGTGG CACAAACACA GAACGCCCGG ACACGCCCAC AAATACGCCA
  241 AATGCACCAG GAAGGAAAAG CTGGGGAAAG GGAAAATGGA AGTCAAAGAA ATGCAAATAT
  301 TCTTTCAAAT GTGTGAACAG CCTCAAGGAA GATCATAACC AGCCATTGTT TGGAGTTCAG
  361 TTTAACTGGC ACAGTAAAGA AGGAGACCCT CTGGTGTTTG CAACTGTGGG AAGCAACAGA
  421 GTAACCTTAT ACGAATGCCA TTCACAGGGG GAGATACGGT TATTGCAGTC CTATGTAGAT
  481 GCTGATGCAG ATGAAAACTT TTACACTTGT GCATGGACCT ATGATAGCAA CACCAGCCAC
  541 CCTCTATTAG CAGTTGCTGG ATCTAGAGGC ATTATAAGAA TAATTAATCC TATAACAATG
  601 CAGTGTATAA AGCACTATGT TGGCCATGGA AATGCTATCA ATGAGCTGAA ATTCCACCCA
  661 CGAGACCCAA ACCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGGTT ATGGAATATC
  721 CAAACAGACA CTCTTGTGGC AATATTCGGA GGTGTGGAAG GGCACAGAGA TGAAGTTCTG
  781 AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCCT GTGGTATGGA TCACTCTCTT
  841 AAACTGTGGA GAATCAACTC AAAGAGGATG ATGAATGCAA TTAAGGAGTC TTATGATTAT
  901 AACCCAAACA AAACTAACAG GCCATTTATT TCCCAGAAAA TCCACTTTCC TGACTTTTCT
  961 ACCAGAGACA TACATAGGAA TTATGTTGAT TGTGTGCGAT GGTTAGGCGA TTTGATACTT
 1021 TCCAAGTCTT GTGAAATGCC ATTGTATGCT GGAAAACCTG GCAAATGGGA GGATGAATAT
 1081 AGAATAAACT CAACCTAGGT GAGGTCTATG GTGACCTATC TGGGCGAATT GGAATTTACA
1141 GCCCAGTGTG ACATTTTTGG TTTACATTGA GGGCT
//
Primer: pMXs-3LTRup_R2
Sequence file: RDB14059_A6Dcb.seq check
>D03255E5_A6Dc_2_pMXs-3LTRup_R2_G12_21.ab1
    1 GCGGGTAAAA TCTTTTATTT TATCGTCGAG TTATCGAAGT CGATCCCATC GCCAAATGCT
   61 GGCATCATCG CAGACAGCTA TGAGGATGCT GCTATCCCTA CTGAAACTGG TTTGTCGAAT
  121 AGCCGCGCCA CATTTATGAT GGGTCAGTGT TGTGCATTTG GCTTTATGAG GATCTTCTAC
  181 TTCTAAATCC CAAACATACA GTTTGCCAAC CTGATTGCCC AATGCAAGCA TCTTTTGCCA
  241 GAAATCCATA GAAAACCTCA TGTACCAAAT GTCACACTGG CTGTAATCAA ATCGCCCAAG
  301 AATAGTCACA TTAGACTCAC TAGGTTTAAT TTTATCTATA TCATCCTCCA TTTTGCCAGG
  361 TTTCCAGCAT ACAATGGCAT TTTCACAAGA CTTGGAAAGT ATCAAATCGC CTAACCATCG
  421 CACACAATCA ACATAATTCC TATGTATGTC TCTGGTAGAA AAGTCAGGAA AGTGGATTTT
  481 CTGGGAAATA AATGGCCTGT TAGTTTTGTT TGGGTTATAA TCATAAGACT CCTTAATTGC
  541 ATTCATCATC CTCTTTGAGT TGATTCTCCA CAGTTTAAGA GAGTGATCCA TACCACAGGA
  601 CATTATTTTT TCACCCAAAA GATCATAATC AGCACTCAGA ACTTCATCTC TGTGCCCTTC
  661 CACACCTCCG AATATTGCCA CAAGAGTGTC TGTTTGGATA TTCCATAACC GTAAAGCATG
  721 ATCTTTACTT ACTGACAGGA GAAGGTTTGG GTCTCGTGGG TGGAATTTCA GCTCATTGAT
  781 AGCATTTCCA TGGCCAACAT AGTGCTTTAT ACACTGCATT GTTATAGGAT TAATTATTCT
  841 TATAATGCCT CTAGATCCAG CAACTGCTAA TAGAGGGTGG CTGGTGTTGC TATCATAGGT
  901 CCATGCACAA GTGTAAAAGT TTTCATCTGC ATCAGCATCT ACATAGGACT GCAATAACCC
  961 GTATCTCCCC CTGTGAATGG CATTCGTATA AGGTTACTCT GTTGCTTTCC CACAGTTGCA
 1021 ACACCAGAGG TCTCTTCTTT ACTGTGCCAG TAAACTGACT CAAACATGCT GGGTTATGAT
 1081 CTCGTGAGCT GTTCACACAT TGAAGAATAT TGCAATTTCT GACCTCAATT TCCCTTTTTC
1141 CCAAGCCAT
//
Primer: Amp_R
Sequence file: RDB14059_A6Dcc.seq check
>D03255E5-2_A6Dc_Amp_R_E01_05_ABI08.ab1
    1 CTCCCTTTTT TAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
   61 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
  121 GCCACCTGCA TAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA
  181 TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAAA AGTTCAGATC AAGGTCAGGA
  241 ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC
  301 GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
  361 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCAGCCCT
  421 CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC
  481 TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT
  541 CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATTGAC
  601 TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG
  661 TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT
  721 TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC
  781 GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG
  841 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG
  901 GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG GGAGACGTCC CAGGGACTTC
  961 GGGGGCCGTT TTTGTGGCCC GACCTGAGTC CAAAAAATCC CGATCGTTTT TGGACTCTTT
 1021 GGTGCACCCC CCCTAATAAG GAGGGATATG TGGATTCTGG TAGGGAGACG AGAACCTAAA
 1081 ACAGTTCCGC TTCCGTTCTG AAATTTTGCT TCCGGATTGG ACGGAAGCCG CGCCGCGCGG
1141 TCTTTATTCT GGCCTGGCCA G
//
Primer: pMFG_1
Sequence file: RDB14059_A6Dcd.seq check
>D03255E5-2_A6Dc_pMFG_1_F01_06_ABI08.ab1
    1 GGATGTGGGA AAGGGGCTGT TAGTACTCTT GTCATGTCAG GGTCAGGGAA GTTTACAAGG
   61 GGCGGGGGTG CCCCATATAA GATCTCATAT GGCCATATGG GGGCGCCTAG AGAAGGAGTG
  121 AGGGCTGGAT AAAGGGAGGA TCGAGGCGGG GTCGAACGAG GAGGTTCAAG GGGGAGAGAC
  181 GGGGCGGATG GAGGAAGAGG AGGCGGAGGC TTAGGGTGTA CAAAGGGCTT GACCCAGGGA
  241 GGGGGGTCAA AAGCCAAGGC TTCCCAGGTC ACGATGTAGG GGACCTGGTC TGGGTGTCCA
  301 TGCGGGCCAG GTGAAAAGAC CTTGATCTTA ACCTGGGTGA TGAGGTCTCG GTTAAAGGTG
  361 CCGTCTCGCG GCCATCCGAC GTTAAAGGTT GGCCATTCTG CAGAGCAGAA GGTAACCCAA
  421 CGTCTCTTCT TGACATCTAC CGACTGGTTG TGAGCGATCC GCTCGACATC TTTCCAGTGA
  481 CCTAAGGTCA AACTTAAGGG AGTGGTAACA GTCTGGCCCT AATTTTCAGA CAAATACAGA
  541 AACACAGTCA GACAGAGACA ACACAGAACG ATGCTGCAGC AGACAAGACG CGCGGCGCGG
  601 CTTCGGTCCC AAACCGAAAG CAAAAATTCA GACGGAGGCG GGAACTGTTT TAGGTTCTCG
  661 TCTCCTACCA GAACCACATA TCCCTCCTAT TAGGGGGGTG CACCAAAGAG TCCAAAACGA
  721 TCGGGATTTT TGGACTCAGG TCGGGCCACA AAAACGGCCC CCGAAGTCCC TGGGACGTCT
  781 CCCAGGGTTG CGGCCGGGTG TTCCGAACTC GTCAGTTCCA CCACGGGTCC GCCAGATACA
  841 GAGGCTAGTT AGGCTAACTA GTACCGACGC AGGCGCATAA AATCAGTCAT AGACACTAGA
  901 CAAATCGGAC AGACACAGAT AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG
  961 TTCCCTGGGC AGGGGTCTCC CGGATCCCGG ACGAGCCCCC AAATGAAAGA CCCCCCGCTG
 1021 GACGGTAGTT CATCACTCAG AGGAGACCCT TCCCCAAGGA ACAGCGGAGA CCCACAAGGT
 1081 TCGGATGCAA CTGGCAGAGG TTTTATTTGG ATACACGGGT AACCCGGGGC GACCTCTCAG
1141 TCCAAATCT
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB14059_A6Dce.seq check
>D03255E5-2_A6Dc_SV40pA3'ElementReverse_G01_07_ABI08.ab1
    1 GGTTCAAGGG GGGGGCGGAG CCTATGGAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
   61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
  121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
  181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
  241 CGCGTTGGCC GATTCATTAA TGCAACTATG GCCATTTAAT GTAAATACTT AAGAAAAAAA
  301 ACCAAATTAA TTTTGATACA TGCTGCATGT GAAAGACCCC CGCTGACGGG TAGTCAATCA
  361 CTCAGAGGAG ACCCTCCCAA GGAACAGCGA GACCACAAGT CGGATGCAAC TGCAAGAGGG
  421 TTTATTGGAT ACACGGGTAC CCGGGCGACT CAGTCAATCG GAGGACTGGC GCCCCGAGTG
  481 AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG GGAGCAGAAG CGCGCGAACA GAAGCGAGAA
  541 GCGAACTGAT TGGTTAGTTC AAATAAGGCA CAGGGTCATT TCAGGTCCTT GGGGCACCCT
  601 GGAAACATCT GATGGTTCTC TAGAAACTGC TGAGGGCTGG ACCGCATCTG GGGACCATCT
  661 GTTCTTGGCC CTGAGCCGGG GCAGGAACTG CTTACCACAG ATATCCTGTT TGGCCCATAT
  721 TCAGCTGTTC CATCTGTTCT TGGCCCTGAG CCGGGGCAGG AACTGCTTAC CACAGATATC
  781 CTGTTTGGCC CATATTCAGC TGTTCCATCT GTTCCTGACC TTGATCTGAA CTTCTCTATT
  841 CTCAGTTATG TATTTTTCCA TGCCTTGCAA AATGGCGTTA CTTAAGCTAG CTTGCCAAAC
  901 CTACAGGTGG GGGTCTTTCA TTCCCCCCCT TTTTTCTGGA GACTAAATAA AATCTTTTTA
  961 TTTTTATCGT CGAGTTTATC GAAGTCGATC CCATCGCCCA AATGCTGGGC ATCATCGCAG
 1021 ACAGCTATGA GGGATGCTGC TTATCCCTAC TGAAACTGGG TTTGTTCGAA TAGGCCGCGC
 1081 ACATTTTATG ATGGGTTCAG TTGTGTGCCA TTTTGGCCTT TATGGAAGGG AATCATTCCT
1141 TACCGTTCCG TA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]