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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pgRNA_mMecp2_T1

Expression vector of sgRNA of mouse Mecp2.

Catalog number RDB14014
Resource name pgRNA_mMecp2_T1
Clone info. Expression vector of sgRNA for CRISPR-CAS9, targeting exon2 of mouse Mecp2.
Comment Expression was confirmed by the depositor.
Vector backbone gRNA_AAVS1-T1 (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mecp2 genomic DNA human U6 genomic DNA
Depositor|Developer Yagita, Kazuhiro |
Other clones in our bank
External Database
mouse Mecp2 human U6
        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)。
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        RDB14014 pgRNA_mMecp2_T1 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14014_A6D7p1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: T7_20bp
        Sequence file: RDB14014_A6D7a.seq check
        >D03336A2_A6D7_1_T7_20bp_B11_05.ab1
            1 TCCTTTGATG CATGCTCGAG CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCGCCCTTT
           61 GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC TGGATCCGGT ACCAAGGTCG
          121 GGCAGGAAGA GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG
          181 TTAGAGAGAT AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT
          241 GACGTAGAAA GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG
          301 ACTATCATAT GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT
          361 GGAAAGGACG AAACACCGAT AATGGAGCGC CGCTGTTGTT TTAGAGCTAG AAATAGCAAG
          421 TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTT
          481 CTAGACCCAG CTTTCTTGTA CAAAGTTGGC ATTAAAGGGC GAATTCCAGC ACACTGGCGG
          541 CCGTTACTAG TGGATCCGAG CTCGGTACCA AGCTTGATGC ATAGCTTGAG TATTCTATAG
          601 TGTCACCTAA ATAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
          661 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
          721 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
          781 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
          841 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
          901 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC ATGGGATAAC
          961 GCAGGAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GGAACCCGTA AAAGGCCGCG
         1021 TTTGCTGGGC GTTTTTCCAT AGGCTCCCGC CCCCCCCTGA ACGAAGCAAT CACAAAAATC
         1081 GGACCGCTCA GGTTCAGAAG TTGGCCGGAA CCCGACCAGG ACTAATAAGA TTACCCAAGG
        1141 C
        //

        Please visit Sequencing and PCR primers for primer information.

        References

        Original, user report and related articles

        original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl