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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pgRNA_mMecp2_T1 (#RDB14014)

Expression vector of sgRNA of mouse Mecp2.

Clone info. Expression vector of sgRNA for CRISPR-CAS9, targeting exon2 of mouse Mecp2.
Comment Expression was confirmed by the depositor.
Vector backbone gRNA_AAVS1-T1 (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mecp2 genomic DNA human U6 genomic DNA
Depositor|Developer Yagita, Kazuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14014 pgRNA_mMecp2_T1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pgRNA_mMecp2_T1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14014).

Reference section:

Tsuchiya, Y., Minami, Y., Umemura, Y., Watanabe, H., Ono, D., Nakamura, W., Takahashi, T., Honma, S., Kondoh, G., Matsuishi, T., Yagita, K., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14014_A6D7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB14014_A6D7a.seq check
>D03336A2_A6D7_1_T7_20bp_B11_05.ab1
    1 TCCTTTGATG CATGCTCGAG CGGCCGCCAG TGTGATGGAT ATCTGCAGAA TTCGCCCTTT
   61 GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC TGGATCCGGT ACCAAGGTCG
  121 GGCAGGAAGA GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG
  181 TTAGAGAGAT AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT
  241 GACGTAGAAA GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG
  301 ACTATCATAT GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT
  361 GGAAAGGACG AAACACCGAT AATGGAGCGC CGCTGTTGTT TTAGAGCTAG AAATAGCAAG
  421 TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTT
  481 CTAGACCCAG CTTTCTTGTA CAAAGTTGGC ATTAAAGGGC GAATTCCAGC ACACTGGCGG
  541 CCGTTACTAG TGGATCCGAG CTCGGTACCA AGCTTGATGC ATAGCTTGAG TATTCTATAG
  601 TGTCACCTAA ATAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
  661 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
  721 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
  781 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
  841 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
  901 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC ATGGGATAAC
  961 GCAGGAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GGAACCCGTA AAAGGCCGCG
 1021 TTTGCTGGGC GTTTTTCCAT AGGCTCCCGC CCCCCCCTGA ACGAAGCAAT CACAAAAATC
 1081 GGACCGCTCA GGTTCAGAAG TTGGCCGGAA CCCGACCAGG ACTAATAAGA TTACCCAAGG
1141 C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]