Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C-HERC2 (#RDB13976)

Expression vector of EGFP-tagged human HERC2.

Clone info. Expression vector of EGFP-tagged human HERC2.
Vector backbone pEGFP-C (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human HERC2 cDNA
Depositor|Developer Imai, Yuzuru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13976 pEGFP-C-HERC2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C-HERC2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13976).

Reference section:

Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13976_A6C2p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C
Sequence file: RDB13976_A6C2a.seq check
>D03172A5_A6C2_2_EGFP_C_A12_03.ab1
    1 CCGGAATCTC GGGCATGGAC GAGCTGTACA AGTACTCAGA TCTCGACATG GCGGCAGGGT
   61 CGGGTGGGAG TGGGGGCTCT GGGGGAGGCC CTGGACCGGG GCCGGGCGGG GGTGGGGGCC
  121 CCAGCGGGAG CGGCTCAGGA CCGGGGTCCA ACGGGGGTCT GGGCAGCGGC GGGGAACTGC
  181 ACCCGCGCAC TGGGCGCTTG GTGAGCCTGT CGGCCTGTGG GCGTACGGCG CGGCGGCAGC
  241 AGCCGGGCCA GGAGTTTAAC CACGGGCTGG TGTTGAGCCG AGAACCCTTG CGCGATGGAC
  301 GCGTCTTCAC CGTCCGCATC GACCGCAAGG TCAACTCCTG GAGCGGCTCC ATTGAGATTG
  361 GGGTGACAGC GCTGGACCCC AGTGTGCTGG ACTTTCCAAG CAGTGCCACG GGCCTGAAGG
  421 GGGGCTCGTG GGTAGTGTCG GGCTGCTCTG TGCTGAGAGA TGGACGCTCT GTGTTGGAGG
  481 AGTATGGTCA GGACCTGGAC CAGCTTGGTG AAGGGGACCG CGTGGGCGTG GAGCGCACAG
  541 TTGCTGGGGA GCTTCGGCTC TGGGTGAATG GGCGGGATTG CGGTGTGGCT GCCACAGGCC
  601 TGCCCCCTCG TGTCTGGGCC GTCGTGGACC TTTATGGCAA GTGCACCCAG ATCACCGTGC
  661 TACCCCCTGA GCCAGGCTTC AGCCCCCCTA CTCCCATCCC CACACCTCCC CTCGAGCCCT
  721 TGGCCCCCAC TGAAGACTCT GCCTTGGCTG AACAGGGGAC CTCTGCAGAT GAAGCCTTCA
  781 TGGTGTCCCC AGCGCAGGCC CGGCCGGAGA CGTTTCCTAA CAGCCTTGAG TCGCATAATG
  841 ACTTTGCCAA CATGGAGCTG TCTGAGGTGG TGAGCAACAC CATCCTGTCT GCCTACAATG
  901 GAGGGCTCCT GAATGTGAAC CCTGAGCTCC CCACCGGGCA GGGATGGCCT GTGATCTAGC
  961 GGTGCTGCCA CCTCGCCCAT TCTCACTTTC CAACGATGCC CTGCTCTTTC ATGAAAAGTG
 1021 CGGGACCCTT CAATCAAACT CAGCAACATT AATTAGGACG CCTGACGCCA CCGCCCTGGA
1081 TGATCACATG GATGTCATGA CCAATCGCCC CCATTTCCG
//
Primer: SV40polyA-R
Sequence file: RDB13976_A6C2b.seq check
>D03172A5_A6C2_2_SV40polyA_R_B12_06.ab1
    1 AGAATTGGAG TTTTAAGCAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT
   61 TATCTAGAGC GGCCGCTGTC TCACCCCTCA TTCCACCCGT ACCAGCAGGG CACAGAGGAG
  121 TGTGGCCCCC TTCTCCTTAG TGACCCACTC AAGTTCGGCT GGACTGAAGT GAGGGTCAGG
  181 AGGTTCTCCA AGGGCAGCGG AAGGGGGTCC CGGGGTGTAG GAGCCAGGGC GCACACACAC
  241 CTGGAACGCC ACCTGAGCCT GGTGCGTCCG CTGGGATTTG GGGTCCCGGA ATTGCACTTT
  301 GGAGGCCAGG GTCTCCGCCC CAGCATATTG AAGGGAGGGG GAAAGCAGCA CAGGAGGGGG
  361 CTGCTCCTCC CGAGGAGGTG CACAGTTCTC GCCAGGCTCC TCGAACCCTA CCCCAGGTTC
  421 TCCCTTCAAA GGACGGCAGC TCAGGATGGA GGCAGTACCT GCTCCCAGCT CCCCTCGGTC
  481 CAGCACTCTC CGTACAGCGG CAACATTGCT CCCGTGATAT GCCATGTGCC ACTTCTTGGT
  541 TAGTGTCCCA GCCTCCAGGC GGGGATTCAC TCTGAGGTTG AACCTGCACC AGCCAAAGGG
  601 CAGTGCATAT TCCCGGGGAG GCTCCCCTCT GCGCCTGTGG GCCTCGTCTC CTCGCAGCTT
  661 CCGGCAAGAC TCACAGTAGC ACAGGCTTCG CTTTGGCGGA GGCATGAAAT AATCTTCGGG
  721 AAGCAGCAGG AGTTCTTGGA AGCGAGAGCA AAGGGCATGG TACTCGCAGC TCTTTAGAGG
  781 ACTAGCGGCA GTGGTGGACC CCAGCACACA CTCTCTTTGA TACCGTCCAC CATGTCTGCT
  841 TTCTCCATGT CCCCTTGGGT TCCAGCACTT TTCCCACTGG CAGCCCCTGG CTCAGGGTTC
  901 ACGATTGTCA CCCTGCTTCA CACTGCCCAT AGAGTCCACA AGCGCATGGC AGGCTGGGGC
  961 ACATCTGGCA CAGCTACCCC TGGTCCACCC CATTACATGG AAGATGCAGC CCCCCCAAAG
 1021 CCTGTCAGCC GCAGTTCCAG ATTGATGCCT TCCAGCACGT GTCCAGATTG GGGCCAAACT
1081 CTCGCGATCT TGAGACCGAT GGTGGGAGAA GCTCCACACG
//
Primer: CMV-Forward
Sequence file: RDB13976_A6C2c.seq check
>D03172A5_A6C2_2_CMV_Forward_C12_09.ab1
    1 CTTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAC TCAGATCTCG
  841 ACATGGCGGC AGGGTCGGGT GGGAGTGGGG GCTCTGGGGG AGGCCCTGGA CCGGGGCCGG
  901 GCGGGGGTGG GGGCCCCAGC GGGAGCGGCT CAGACCGGGG TCCAACGGGG GTCTGGCAGC
  961 GGCGGGGACT GCACCCGCGC ACTGGGCGCT GTGAGCTGTC GCCTGTGGGC GTACCGCGCG
 1021 CCGCAGCAGC CGGGCCAGAG TTACCACGGC CTGAGTGAGC CGAGACCCTG CTCGATGGCG
1081 CCGATCTTCA
//
Primer: SV40pro_F_V2
Sequence file: RDB13976_A6C2d.seq check
>D03172A5_A6C2_2_SV40pro_F_V2_D12_12.ab1
    1 GAGGGCTTTT TGGAGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA GTTCTTCTGA GCGGGACCTC TGGGGTTCGA ATGACGACAG CGACGCCCAA
  901 CTGCATCACG AGATTCGATT CCACGCCGCC TTCTATGAAG GTGGGCTCGA TTCGATCGGG
961 ACGCAGCTGG ATGATCTCAA CCGGGATCCA TGCCTGCATC TGCACACTAG GAGTCCTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]