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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-NEURL4 (#RDB13975)

Expression vector of human NEURL4.

Clone info. Expression vector of human NEURL4.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Gene/insert name human NEURL4 cDNA
Depositor|Developer Imai, Yuzuru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13975 pEF-NEURL4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-NEURL4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13975).

Reference section:

Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13975_A6C2p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB13975_A6C2a.seq check
>D03172A3_A6C2_1_EF1a_C_F_G11_20.ab1
    1 CCTGGATTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGTCGAGAT CCACTAGTAA
  121 CGGCCGCCAG TGTGCTGGAA TTCCTCGACA TGGCGGCAGG GTCGGGTGGG AGTGGGGGCT
  181 CTGGGGGAGG CCCTGGACCG GGGCCGGGCG GGGGTGGGGG CCCCAGCGGG AGCGGCTCAG
  241 GACCGGGGTC CAACGGGGGT CTGGGCAGCG GCGGGGAACT GCACCCGCGC ACTGGGCGCT
  301 TGGTGAGCCT GTCGGCCTGT GGGCGTACGG CGCGGCGGCA GCAGCCGGGC CAGGAGTTTA
  361 ACCACGGGCT GGTGTTGAGC CGAGAACCCT TGCGCGATGG ACGCGTCTTC ACCGTCCGCA
  421 TCGACCGCAA GGTCAACTCC TGGAGCGGCT CCATTGAGAT TGGGGTGACA GCGCTGGACC
  481 CCAGTGTGCT GGACTTTCCA AGCAGTGCCA CGGGCCTGAA GGGGGGCTCG TGGGTAGTGT
  541 CGGGCTGCTC TGTGCTGAGA GATGGACGCT CTGTGTTGGA GGAGTATGGT CAGGACCTGG
  601 ACCAGCTTGG TGAAGGGGAC CGCGTGGGCG TGGAGCGCAC AGTTGCTGGG GAGCTTCGGC
  661 TCTGGGTGAA TGGGCGGGAT TGCGGTGTGG CTGCCACAGG CCTGCCCCCT CGTGTCTGGG
  721 CCGTCGTGGA CCTTTATGGC AAGTGCACCC AGATCACCGT GCTACCCCCT GAGCCAGGCT
  781 TCAGCCCCCC TACTCCCATC CCCACACCTC CCCTCGAGCC CTTGGCCCCC ACTGAAGACT
  841 CTGCCTTGGC TGAACAGGGA CCTCTGCAGA TGAAGCCTTT CATGGTGTCC CCAGCGCAGC
  901 CCGGCCGGAG ACGTTTCCTA ACAGCCTGAG TCGCATATGA CTTTGCACAT GAGCTGTCTG
  961 AGTGTGAGCC ACACATCTGT CTGCTTACAT TGAGGCTCTG ATGTGAACTG AACTCCCACG
1021 CCAGGGAGGC CTGGATCTAC GGGTCTGCAC TGCATCTCTC ACGATGCCTG CTCC
//
Primer: Poly(A) region primer #0
Sequence file: RDB13975_A6C2b.seq check
>D03172A3_A6C2_1_GNPmixture_F01_16.ab1
    1 GGGTAATAAT CCGTGAAAGT TTAAGTTAAA TGTGACAGTG GAGGGGACAC TGGTGAGTGG
   61 GGGGGTGAAG AGGTGGAGGG TGACAGTGGA GCTTCCTTTC ACACACAGGC CTGACATGAT
  121 TCCTCCTGTC TGCTCCCTCC CACATCCCCA GTCCCCGTCC AGCAAGGCAA ATGTCCAGGC
  181 ACCTGCCTGG CCCTAAAAAG AGTCACAGTC CTGACCCTCG TGGGGGCAGG GCAGGTGTCC
  241 CGAGGCCACC CAGAAAAACA GGAGACACGT CGGTGATGTT CGGGAGTCAA ACCATGTCCC
  301 AAAAGTCTTA AGAAGAAAAA CGAGATTCCC CAGCAAATTC GTGGGACCCC AGGGCCATGC
  361 CTCCCAGCTC TGGCCACCTC TGCTTGTCCA GGGGCCTCAC AGCCAAGGGG ATGCAGGGGC
  421 CGCTGTGCAG TCGGCTGAGG CCAGAGTGAG GGGTGCAAGG ACCCAGATGG GGAACACTGC
  481 TGTTTAAATA TTAAACAGGG ATTTCTTGTC TCCCACGTGG GAGACCTGAT ACTCTCAAGG
  541 GTCCCAGGTG GGGACCCTCA CTCTAGGCTT TACTTGTACA GCGGCCGCTG TCTCACCCCT
  601 CATTCCACCC GTACCAGCAG GGCACAGAGG AGTGTGGCCC CCTTCTCCTT AGTGACCCAC
  661 TCAAGTTCGG CTGGACTGAA GTGAGGGTCA GGAGGTTCTC CAAGGGGCAG CGGAAGGGGG
  721 TCCCGGGGTG TAGGGAGCCA GGGCGCACAC ACACCTGGAA CACCCACCTG GAGCCCTGGT
  781 GCGTCCGCTT GGGATTTTGG GGTCCCGGAA ATTGCACTTT TGGAGGCCAG GGTCTCCGCC
841 CCAACATAAT TGAAAGGGAG GGGGGGAAAG CAACACAGGA A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]