Resource data sheet
Prev.

pEF-NEURL4

Expression vector of human NEURL4.

Catalog number RDB13975
Resource name pEF-NEURL4
Clone info. Expression vector of human NEURL4.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Gene/insert name human NEURL4 cDNA
Depositor Imai, Yuzuru |
 
Other clones in our bank human NEURL4 (NCBI Gene 84461) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13975 pEF-NEURL4 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13975_A6C2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB13975_A6C2a.seq
>D03172A3_A6C2_1_EF1a_C_F_G11_20.ab1
    1 CCTGGATTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGTCGAGAT CCACTAGTAA
  121 CGGCCGCCAG TGTGCTGGAA TTCCTCGACA TGGCGGCAGG GTCGGGTGGG AGTGGGGGCT
  181 CTGGGGGAGG CCCTGGACCG GGGCCGGGCG GGGGTGGGGG CCCCAGCGGG AGCGGCTCAG
  241 GACCGGGGTC CAACGGGGGT CTGGGCAGCG GCGGGGAACT GCACCCGCGC ACTGGGCGCT
  301 TGGTGAGCCT GTCGGCCTGT GGGCGTACGG CGCGGCGGCA GCAGCCGGGC CAGGAGTTTA
  361 ACCACGGGCT GGTGTTGAGC CGAGAACCCT TGCGCGATGG ACGCGTCTTC ACCGTCCGCA
  421 TCGACCGCAA GGTCAACTCC TGGAGCGGCT CCATTGAGAT TGGGGTGACA GCGCTGGACC
  481 CCAGTGTGCT GGACTTTCCA AGCAGTGCCA CGGGCCTGAA GGGGGGCTCG TGGGTAGTGT
  541 CGGGCTGCTC TGTGCTGAGA GATGGACGCT CTGTGTTGGA GGAGTATGGT CAGGACCTGG
  601 ACCAGCTTGG TGAAGGGGAC CGCGTGGGCG TGGAGCGCAC AGTTGCTGGG GAGCTTCGGC
  661 TCTGGGTGAA TGGGCGGGAT TGCGGTGTGG CTGCCACAGG CCTGCCCCCT CGTGTCTGGG
  721 CCGTCGTGGA CCTTTATGGC AAGTGCACCC AGATCACCGT GCTACCCCCT GAGCCAGGCT
  781 TCAGCCCCCC TACTCCCATC CCCACACCTC CCCTCGAGCC CTTGGCCCCC ACTGAAGACT
  841 CTGCCTTGGC TGAACAGGGA CCTCTGCAGA TGAAGCCTTT CATGGTGTCC CCAGCGCAGC
  901 CCGGCCGGAG ACGTTTCCTA ACAGCCTGAG TCGCATATGA CTTTGCACAT GAGCTGTCTG
  961 AGTGTGAGCC ACACATCTGT CTGCTTACAT TGAGGCTCTG ATGTGAACTG AACTCCCACG
1021 CCAGGGAGGC CTGGATCTAC GGGTCTGCAC TGCATCTCTC ACGATGCCTG CTCC
//
Primer: Poly(A) region primer #0 RDB13975_A6C2b.seq
>D03172A3_A6C2_1_GNPmixture_F01_16.ab1
    1 GGGTAATAAT CCGTGAAAGT TTAAGTTAAA TGTGACAGTG GAGGGGACAC TGGTGAGTGG
   61 GGGGGTGAAG AGGTGGAGGG TGACAGTGGA GCTTCCTTTC ACACACAGGC CTGACATGAT
  121 TCCTCCTGTC TGCTCCCTCC CACATCCCCA GTCCCCGTCC AGCAAGGCAA ATGTCCAGGC
  181 ACCTGCCTGG CCCTAAAAAG AGTCACAGTC CTGACCCTCG TGGGGGCAGG GCAGGTGTCC
  241 CGAGGCCACC CAGAAAAACA GGAGACACGT CGGTGATGTT CGGGAGTCAA ACCATGTCCC
  301 AAAAGTCTTA AGAAGAAAAA CGAGATTCCC CAGCAAATTC GTGGGACCCC AGGGCCATGC
  361 CTCCCAGCTC TGGCCACCTC TGCTTGTCCA GGGGCCTCAC AGCCAAGGGG ATGCAGGGGC
  421 CGCTGTGCAG TCGGCTGAGG CCAGAGTGAG GGGTGCAAGG ACCCAGATGG GGAACACTGC
  481 TGTTTAAATA TTAAACAGGG ATTTCTTGTC TCCCACGTGG GAGACCTGAT ACTCTCAAGG
  541 GTCCCAGGTG GGGACCCTCA CTCTAGGCTT TACTTGTACA GCGGCCGCTG TCTCACCCCT
  601 CATTCCACCC GTACCAGCAG GGCACAGAGG AGTGTGGCCC CCTTCTCCTT AGTGACCCAC
  661 TCAAGTTCGG CTGGACTGAA GTGAGGGTCA GGAGGTTCTC CAAGGGGCAG CGGAAGGGGG
  721 TCCCGGGGTG TAGGGAGCCA GGGCGCACAC ACACCTGGAA CACCCACCTG GAGCCCTGGT
  781 GCGTCCGCTT GGGATTTTGG GGTCCCGGAA ATTGCACTTT TGGAGGCCAG GGTCTCCGCC
841 CCAACATAAT TGAAAGGGAG GGGGGGAAAG CAACACAGGA A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680.

2018.11.27

GNP_filter3_RDBDEP_html_181012.pl