Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-LRRK2-FLAG-His

Expression vector of FLAG-His-tagged human LRRK2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13973_A6C2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB13973_A6C2a.seq
>D03172A1_A6C2_2_EF1a_C_F_G01_19.ab1     1 ACTTTGGGAA TTGGCCCTTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG    61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTCTCTAG TCGAGATGGC   121 TAGTGGCAGC TGTCAGGGGT GCGAAGAGGA CGAGGAAACT CTGAAGAAGT TGATAGTCAG   181 GCTGAACAAT GTCCAGGAAG GAAAACAGAT AGAAACGCTG GTCCAAATCC TGGAGGATCT   241 GCTGGTGTTC ACGTACTCCG AGCACGCCTC CAAGTTATTT CAAGGCAAAA ATATCCATGT   301 GCCTCTGTTG ATCGTCTTGG ACTCCTATAT GAGAGTCGCG AGTGTGCAGC AGGTGGGTTG   361 GTCACTTCTG TGCAAATTAA TAGAAGTCTG TCCAGGTACA ATGCAAAGCT TAATGGGACC   421 CCAGGATGTT GGAAATGATT GGGAAGTCCT TGGTGTTCAC CAATTGATTC TTAAAATGCT   481 AACAGTTCAT AATGCCAGTG TAAACTTGTC AGTGATTGGA CTGAAGACCT TAGATCTCCT   541 CCTAACTTCA GGTAAAATCA CCTTGCTGAT ATTGGATGAA GAAAGTGATA TTTTCATGTT   601 AATTTTTGAT GCCATGCACT CATTTCCAGC CAATGATGAA GTCCAGAAAC TTGGATGCAA   661 AGCTTTACAT GTGCTGTTTG AGAGAGTCTC AGAGGAGCAA CTGACTGAAT TTGTTGAGAA   721 CAAAGATTAT ATGATATTGT TAAGTGCGTT AACAAATTTT AAAGATGAAG AGGAAATTGT   781 GCTTCATGTG CTGCATTGTT TACATTCCCT AGCGATTCCT TGCAATAATG TGGAAGTCCT   841 CATGAGTGGC AATGTCAGGT GTTATAATAT TGTGGTGGAA GCTATGAAAG CATTCCCTAT   901 GAGTGAAGAA ATTCAAGAAG TGAGTTGCTG TTTGCTCCAT AGGCTTACAT TAGGTAATTT   961 TTTCAATATC CCTGTATTTA ACCGAAGTCC ATGAGTTTGT GGTGAAAGCT GGTGCAGCAG  1021 TACCCAGAGA ATGCCAGCAA TTGCAGAATC TCAGCCGCTC AAGCTGTTTG GTCCTTCTTA 1081 ATCCAAGGAT TTTAGAGGC //
Primer: Poly(A) region primer #0 Sequence file: RDB13973_A6C2b.seq
>D03172A1_A6C2_2_GNPmixture_B01_04.ab1     1 AGGAAAAATC GGTGAAGCTC AGCGTACAAT GTGGCAGTGG AGGGGACACT GGTGAGTGGG    61 GGGTGAAGAG GTGGAGGGTG ACAGTGGAGC TTCCTTTCAC ACACAGGCCT GACATGATTC   121 CTCCTGTCTG CTCCCTCCCA CATCCCCAGT CCCCGTCCAG CAAGGCAAAT GTCCAGGCAC   181 CTGCCTGGCC CTAAAAAGAG TCACAGTCCT GACCCTCGTG GGGGCAGGGC AGGTGTCCCG   241 AGGCCACCCA GAAAAACAGG AGACACGTCG GTGATGTTCG GGAGTCAAAC CATGTCCCAA   301 AAGTCTTAAG AAGAAAAACG AGATTCCCCA GCAAATTCGT GGGACCCCAG GGCCATGCCT   361 CCCAGCTCTG GCCACCTCTG CTTGTCCAGG GGCCTCACAG CCAAGGGGAT GCAGGGGCCG   421 CTGTGCAGTC GGCTGAGGCC AGAGTGAGGG GTGCAAGGAC CCAGATGGGG AACACTGCTG   481 TTTAAATATT AAACAGGGAT TTCTTGTCTC CCACGTGGGA GACCTGATAC TCTCAAGGGT   541 CCCAGGTGGG GACCCTCACT CTAGGCTTTA CTTGTACAGC GGCTAGCATT TAGGTGACAC   601 TATAGAATAG GGCCCTCTAG AGCAGCCAAC TCAGCTTCCT TTCGGGCTTT GTTAGCAGCC   661 GGATCTCAGT GGTGGTGGTG GTGGTGCTCG AAGATCTTGT CATCGTCGTC CTTGTAGTCC   721 TCGACCTCGA CGCGGCCGCA CTCAACAGAT GTTCGTCTCA TTTTTTCAGC TAATTCTTTT   781 CTCACTTCAA TGTGTTTTTC TAAATTTTGC ACTTCATGTG GAAGATTGAT GTCCCAAAAC   841 GGTCAAGCAA GATTGTATCT CTTTCTGCTT TTGTGTACCT TCAGTATTTT TCCGGTTGTA   901 GCCCCAATAC CAAGCATGAC ATTTTTTAAA GGCTTCCCTA AGCTGTGCCT GTCCATCCAA   961 TGACCTCTTG ACCCGAAATT ACCAAAAGGT TGTAAAATTA ACCACCGTTA ATAAAGTCCG  1021 ACCGAAGTTG AAAGATCCCA AGGGAGGTAA AAATATGGGC CCCTCCCTTC CCAGATTCCT 1081 AATCCCCTAG AAGCCAGGTG TTTCCTTTTC CTGACAGAGT CACCCTGAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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2023.08.18

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