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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-LRRK2-FLAG-His

Expression vector of FLAG-His-tagged human LRRK2.

Catalog number RDB13973
Resource name pEF-LRRK2-FLAG-His
Clone info. Expression vector of FLAG-His-tagged human LRRK2.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Gene/insert name human LRRK2 cDNA
Depositor|Developer Imai, Yuzuru |
Other clones in our bank

External Database
human LRRK2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。
          付加的提供条件:
          pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13973 pEF-LRRK2-FLAG-His DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-LRRK2-FLAG-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13973).

          Reference section:

          Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13973_A6C2p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB13973_A6C2a.seq check
          >D03172A1_A6C2_2_EF1a_C_F_G01_19.ab1
              1 ACTTTGGGAA TTGGCCCTTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
             61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG AATTCTCTAG TCGAGATGGC
            121 TAGTGGCAGC TGTCAGGGGT GCGAAGAGGA CGAGGAAACT CTGAAGAAGT TGATAGTCAG
            181 GCTGAACAAT GTCCAGGAAG GAAAACAGAT AGAAACGCTG GTCCAAATCC TGGAGGATCT
            241 GCTGGTGTTC ACGTACTCCG AGCACGCCTC CAAGTTATTT CAAGGCAAAA ATATCCATGT
            301 GCCTCTGTTG ATCGTCTTGG ACTCCTATAT GAGAGTCGCG AGTGTGCAGC AGGTGGGTTG
            361 GTCACTTCTG TGCAAATTAA TAGAAGTCTG TCCAGGTACA ATGCAAAGCT TAATGGGACC
            421 CCAGGATGTT GGAAATGATT GGGAAGTCCT TGGTGTTCAC CAATTGATTC TTAAAATGCT
            481 AACAGTTCAT AATGCCAGTG TAAACTTGTC AGTGATTGGA CTGAAGACCT TAGATCTCCT
            541 CCTAACTTCA GGTAAAATCA CCTTGCTGAT ATTGGATGAA GAAAGTGATA TTTTCATGTT
            601 AATTTTTGAT GCCATGCACT CATTTCCAGC CAATGATGAA GTCCAGAAAC TTGGATGCAA
            661 AGCTTTACAT GTGCTGTTTG AGAGAGTCTC AGAGGAGCAA CTGACTGAAT TTGTTGAGAA
            721 CAAAGATTAT ATGATATTGT TAAGTGCGTT AACAAATTTT AAAGATGAAG AGGAAATTGT
            781 GCTTCATGTG CTGCATTGTT TACATTCCCT AGCGATTCCT TGCAATAATG TGGAAGTCCT
            841 CATGAGTGGC AATGTCAGGT GTTATAATAT TGTGGTGGAA GCTATGAAAG CATTCCCTAT
            901 GAGTGAAGAA ATTCAAGAAG TGAGTTGCTG TTTGCTCCAT AGGCTTACAT TAGGTAATTT
            961 TTTCAATATC CCTGTATTTA ACCGAAGTCC ATGAGTTTGT GGTGAAAGCT GGTGCAGCAG
           1021 TACCCAGAGA ATGCCAGCAA TTGCAGAATC TCAGCCGCTC AAGCTGTTTG GTCCTTCTTA
          1081 ATCCAAGGAT TTTAGAGGC
          //
          Primer: Poly(A) region primer #0
          Sequence file: RDB13973_A6C2b.seq check
          >D03172A1_A6C2_2_GNPmixture_B01_04.ab1
              1 AGGAAAAATC GGTGAAGCTC AGCGTACAAT GTGGCAGTGG AGGGGACACT GGTGAGTGGG
             61 GGGTGAAGAG GTGGAGGGTG ACAGTGGAGC TTCCTTTCAC ACACAGGCCT GACATGATTC
            121 CTCCTGTCTG CTCCCTCCCA CATCCCCAGT CCCCGTCCAG CAAGGCAAAT GTCCAGGCAC
            181 CTGCCTGGCC CTAAAAAGAG TCACAGTCCT GACCCTCGTG GGGGCAGGGC AGGTGTCCCG
            241 AGGCCACCCA GAAAAACAGG AGACACGTCG GTGATGTTCG GGAGTCAAAC CATGTCCCAA
            301 AAGTCTTAAG AAGAAAAACG AGATTCCCCA GCAAATTCGT GGGACCCCAG GGCCATGCCT
            361 CCCAGCTCTG GCCACCTCTG CTTGTCCAGG GGCCTCACAG CCAAGGGGAT GCAGGGGCCG
            421 CTGTGCAGTC GGCTGAGGCC AGAGTGAGGG GTGCAAGGAC CCAGATGGGG AACACTGCTG
            481 TTTAAATATT AAACAGGGAT TTCTTGTCTC CCACGTGGGA GACCTGATAC TCTCAAGGGT
            541 CCCAGGTGGG GACCCTCACT CTAGGCTTTA CTTGTACAGC GGCTAGCATT TAGGTGACAC
            601 TATAGAATAG GGCCCTCTAG AGCAGCCAAC TCAGCTTCCT TTCGGGCTTT GTTAGCAGCC
            661 GGATCTCAGT GGTGGTGGTG GTGGTGCTCG AAGATCTTGT CATCGTCGTC CTTGTAGTCC
            721 TCGACCTCGA CGCGGCCGCA CTCAACAGAT GTTCGTCTCA TTTTTTCAGC TAATTCTTTT
            781 CTCACTTCAA TGTGTTTTTC TAAATTTTGC ACTTCATGTG GAAGATTGAT GTCCCAAAAC
            841 GGTCAAGCAA GATTGTATCT CTTTCTGCTT TTGTGTACCT TCAGTATTTT TCCGGTTGTA
            901 GCCCCAATAC CAAGCATGAC ATTTTTTAAA GGCTTCCCTA AGCTGTGCCT GTCCATCCAA
            961 TGACCTCTTG ACCCGAAATT ACCAAAAGGT TGTAAAATTA ACCACCGTTA ATAAAGTCCG
           1021 ACCGAAGTTG AAAGATCCCA AGGGAGGTAA AAATATGGGC CCCTCCCTTC CCAGATTCCT
          1081 AATCCCCTAG AAGCCAGGTG TTTCCTTTTC CTGACAGAGT CACCCTGAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

          2023.08.18

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