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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-human yes-associated protein (YAP)1 (#RDB13968)

Expression vector of FLAG-tagged YAP1.

Clone info. Expression vector of FLAG-tagged YAP1.
Vector backbone pCMV-FLAG2 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name human YAP1 cDNA
Depositor|Developer Hata, Yutaka |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13968 pFLAG-human yes-associated protein (YAP)1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-human yes-associated protein (YAP)1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13968).

Reference section:

Kawano, S., Maruyama, J., Nagashima, S., Inami, K., Qiu, W., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13968_A6Btp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,insert 5'
Sequence file: RDB13968_A6Btc.seq check
>D03116A2_A6Bt_1_CMV_Forward_H05_23.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCG TTTAGTGAAC CGTCAGAATT GATCTACCAT GGACTACAAA GACGATGACG
  121 ACAAGCTTGC GGCCGCGAAT TCATCGATAG ATCCCGGGCA GCAGCCGCCG CCTCAACCGG
  181 CCCCCCAGGG CCAAGGGCAG CCGCCTTCGC AGCCCCCGCA GGGGCAGGGC CCGCCGTCCG
  241 GACCCGGGCA ACCGGCACCC GCGGCGACCC AGGCGGCGCC GCAGGCACCC CCCGCCGGGC
  301 ATCAGATCGT GCACGTCCGC GGGGACTCGG AGACCGACCT GGAGGCGCTC TTCAACGCCG
  361 TCATGAACCC CAAGACGGCC AACGTGCCCC AGACCGTGCC CATGAGGCTC CGGAAGCTGC
  421 CCGACTCCTT CTTCAAGCCG CCGGAGCCCA AATCCCACTC CCGACAGGCC AGTACTGATG
  481 CAGGCACTGC AGGAGCCCTG ACTCCACAGC ATGTTCGAGC TCATTCCTCT CCAGCTTCTC
  541 TGCAGTTGGG AGCTGTTTCT CCTGGGACAC TGACCCCCAC TGGAGTAGTC TCTGGCCCAG
  601 CAGCTACACC CACAGCTCAG CATCTTCGAC AGTCTTCTTT TGAGATACCT GATGATGTAC
  661 CTCTGCCAGC AGGTTGGGAG ATGGCAAAGA CATCTTCTGG TCAGAGATAC TTCTTAAATC
  721 ACATCGATCA GACAACAACA TGGCANGACC CCAGGAANGC CATGCTGTCC CAGATGAACG
  781 TCACAGCCCC CACCAGTCCA CCAGTGCAGC AGAATATGAT GNACTCGGCT TCNNNTCCTC
  841 TTCCTGATGG ATGGGAACAA GCCATGACTC NNGANNGANA AATTTACTAT ATAAANNNTN
  901 AGANNNAGAN NNNCNCNNNG CNANANCNNA NGNCTNNNNC NNCNNTTNNN NNTGNANNNA
961 NNNNANNCAN NCNNNNNNNN NN
//
Primer: CMV24ver2 (Pr0018)
Region: hGH pA,insert 3'
Sequence file: RDB13968_A6Btd.seq check
>D03116A2A6Bt__1_CMV24ver2_D06_12.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNGNNNNCT TCNNGGGCNN GGNNAGGCNC TGGGGNNGGG
   61 TCACAGGGAT GCCACCCGGG NTCCTCTAGA GTCGACTGGT ACCGTCGACT GCAGAATTCA
  121 GTCTGCCTGA GGGCTCTATA ACCATGTAAG AAAGCTTTCT TTATCTAGCT TGGTGGCAGC
  181 CAAAACAGAC TCCATGTCAT TAAGGATGTC AGAACTCAAA GCTTCCTGCA GACTTGGCAT
  241 CAGCTCCTCT CCTTCTATGT TCATTCCATC TCCTTCCAGT GTTCCAAGGT CCACATTTGT
  301 CCCAGGAATG GCTTCAAGGT AGTCTGGGAA ACGGTTCTGC TGTGAGGGCA GGGTGCTTTG
  361 GTTGATAGTA TCACCTGTAT CCATCTCATC CACACTGTTC AGGAAGTCAT CTGGGGTTCG
  421 AGGGACACTG TAGCTGCTCA TGCTTAGTCC ACTGTCTGTA CTCTCATCTC GAGAGTGATA
  481 GGTGCCACTG TTAAGGAAAG GATCTGAGCT ATTGGTCGTC ATTGTTCTCA ATTCCTGAGA
  541 CATCCCGGGA GAAGACACTG GATTTTGAGT CCCACCATCC TGCTCCAGTG TTGGTAACTG
  601 GCTACGCAGG GCTAACTCCT GACATTTTGG AGAATTTGCT GTGCTGGGAT TGATATTCCG
  661 CATTGCCTGC CGAAGCAGTT CTTGCTGTTT CAGCCGCAGC CTCTCCTTCT CCATCTGCAG
  721 TTGCTGCAGT CGCATCTGTT GCTGCTGGTT GGAGTTGCTG CCACCCATGA CGCCTCCCTG
  781 TGGGCTCTGG GGANCCAGGG GTGGTGGCTG TTTCACTGGA GCACTCTGAC TGATTCTCTG
  841 GTTCATGGCA AAACGAGGGT CNAGCCTTGG GTCTAGCCNA GANGTGGTCN TGTTCTTATN
  901 GNTTATATAN TNANTTTCTN CNTCCTGANT CATGGCTTGN TCCCATCCAT CNNNAANANN
  961 NNCTNNNNCC NANTTCNTCA TATTCTGCTG NACTGGNNGN NTGGNNGGGG GCTGNGNNNN
1021 NCNTNNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]