RDB13968 - pFLAG-human yes-associated protein (YAP)1

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pFLAG-human yes-associated protein (YAP)1

Expression vector of FLAG-tagged YAP1.

Catalog number	RDB13968
Resource name	pFLAG-human yes-associated protein (YAP)1
Clone info.	Expression vector of FLAG-tagged YAP1.
Vector backbone	pCMV-FLAG2 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Amp^r
Gene/insert name	human YAP1 cDNA
Depositor\|Developer	Hata, Yutaka \|

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human YAP1

External Database
human YAP1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13968	pFLAG-human yes-associated protein (YAP)1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13968_A6Btp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,FLAG,insert 5' Sequence file: RDB13968_A6Btc.seq
>D03116A2_A6Bt_1_CMV_Forward_H05_23.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCG TTTAGTGAAC CGTCAGAATT GATCTACCAT GGACTACAAA GACGATGACG 121 ACAAGCTTGC GGCCGCGAAT TCATCGATAG ATCCCGGGCA GCAGCCGCCG CCTCAACCGG 181 CCCCCCAGGG CCAAGGGCAG CCGCCTTCGC AGCCCCCGCA GGGGCAGGGC CCGCCGTCCG 241 GACCCGGGCA ACCGGCACCC GCGGCGACCC AGGCGGCGCC GCAGGCACCC CCCGCCGGGC 301 ATCAGATCGT GCACGTCCGC GGGGACTCGG AGACCGACCT GGAGGCGCTC TTCAACGCCG 361 TCATGAACCC CAAGACGGCC AACGTGCCCC AGACCGTGCC CATGAGGCTC CGGAAGCTGC 421 CCGACTCCTT CTTCAAGCCG CCGGAGCCCA AATCCCACTC CCGACAGGCC AGTACTGATG 481 CAGGCACTGC AGGAGCCCTG ACTCCACAGC ATGTTCGAGC TCATTCCTCT CCAGCTTCTC 541 TGCAGTTGGG AGCTGTTTCT CCTGGGACAC TGACCCCCAC TGGAGTAGTC TCTGGCCCAG 601 CAGCTACACC CACAGCTCAG CATCTTCGAC AGTCTTCTTT TGAGATACCT GATGATGTAC 661 CTCTGCCAGC AGGTTGGGAG ATGGCAAAGA CATCTTCTGG TCAGAGATAC TTCTTAAATC 721 ACATCGATCA GACAACAACA TGGCANGACC CCAGGAANGC CATGCTGTCC CAGATGAACG 781 TCACAGCCCC CACCAGTCCA CCAGTGCAGC AGAATATGAT GNACTCGGCT TCNNNTCCTC 841 TTCCTGATGG ATGGGAACAA GCCATGACTC NNGANNGANA AATTTACTAT ATAAANNNTN 901 AGANNNAGAN NNNCNCNNNG CNANANCNNA NGNCTNNNNC NNCNNTTNNN NNTGNANNNA 961 NNNNANNCAN NCNNNNNNNN NN //
Primer: CMV24ver2 Region: hGH pA,insert 3' Sequence file: RDB13968_A6Btd.seq
>D03116A2A6Bt__1_CMV24ver2_D06_12.ab1 1 NNNNNNNNNN NNNNNNNNNG NNNGNNNNCT TCNNGGGCNN GGNNAGGCNC TGGGGNNGGG 61 TCACAGGGAT GCCACCCGGG NTCCTCTAGA GTCGACTGGT ACCGTCGACT GCAGAATTCA 121 GTCTGCCTGA GGGCTCTATA ACCATGTAAG AAAGCTTTCT TTATCTAGCT TGGTGGCAGC 181 CAAAACAGAC TCCATGTCAT TAAGGATGTC AGAACTCAAA GCTTCCTGCA GACTTGGCAT 241 CAGCTCCTCT CCTTCTATGT TCATTCCATC TCCTTCCAGT GTTCCAAGGT CCACATTTGT 301 CCCAGGAATG GCTTCAAGGT AGTCTGGGAA ACGGTTCTGC TGTGAGGGCA GGGTGCTTTG 361 GTTGATAGTA TCACCTGTAT CCATCTCATC CACACTGTTC AGGAAGTCAT CTGGGGTTCG 421 AGGGACACTG TAGCTGCTCA TGCTTAGTCC ACTGTCTGTA CTCTCATCTC GAGAGTGATA 481 GGTGCCACTG TTAAGGAAAG GATCTGAGCT ATTGGTCGTC ATTGTTCTCA ATTCCTGAGA 541 CATCCCGGGA GAAGACACTG GATTTTGAGT CCCACCATCC TGCTCCAGTG TTGGTAACTG 601 GCTACGCAGG GCTAACTCCT GACATTTTGG AGAATTTGCT GTGCTGGGAT TGATATTCCG 661 CATTGCCTGC CGAAGCAGTT CTTGCTGTTT CAGCCGCAGC CTCTCCTTCT CCATCTGCAG 721 TTGCTGCAGT CGCATCTGTT GCTGCTGGTT GGAGTTGCTG CCACCCATGA CGCCTCCCTG 781 TGGGCTCTGG GGANCCAGGG GTGGTGGCTG TTTCACTGGA GCACTCTGAC TGATTCTCTG 841 GTTCATGGCA AAACGAGGGT CNAGCCTTGG GTCTAGCCNA GANGTGGTCN TGTTCTTATN 901 GNTTATATAN TNANTTTCTN CNTCCTGANT CATGGCTTGN TCCCATCCAT CNNNAANANN 961 NNCTNNNNCC NANTTCNTCA TATTCTGCTG NACTGGNNGN NTGGNNGGGG GCTGNGNNNN 1021 NCNTNNNGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969.

2022.05.30

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