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pLL3.7 K122 FH-TAZ-ires-GFP-TEAD-responsive-H2B mCherry (#RDB13967)

A system that monitors directly the TAZ-dependent transcription.

Clone info. A system that monitors directly the TAZ-dependent transcription.
Vector backbone pLL3.7 (plasmid)
Size of vector backbone 7.6 kb
Selectable markers Amp^r
Gene/insert name human TAZ cDNA human H2B cDNA Aequorea victoria GFP Discosoma sp. RFP
Depositor|Developer Hata, Yutaka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
Remarks Remember that you will be working with samples containing infectious virus. An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。

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RDB13967 pLL3.7 K122 FH-TAZ-ires-GFP-TEAD-responsive-H2B mCherry DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pLL3.7 K122 FH-TAZ-ires-GFP-TEAD-responsive-H2B mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13967).

Reference section:

Kawano, S., Maruyama, J., Nagashima, S., Inami, K., Qiu, W., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13967_A6Btp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hTAZ-N_R
Sequence file: RDB13967_A6Bta.seq check
>D03116A1_A6Bt_1_hTAZ-N_R_F12_18.ab1
    1 CGAGAGGCTC GGAGGTCTGT GTCTAGGTCC TGCGTGACGT GGATCACTTG CTGCCCAGGC
   61 GGCGGGAGCG GAGGGGGCGC CGAGGCCGGA TTCATCTTCT GGGCGGGCAG CGAAGCTGAG
  121 CCTGAGCGCG TGAATTCATG ATGATGATGA TGGTGCAATT CCTTGTCGTC ATCGTCTTTG
  181 TAGTCCATGG CCCCGGCTAG CGGATCTGAC GGTTCACTAA ACCAGCTCTG CTTATATAGA
  241 CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG GGCGGAGTTG TTACGACATT
  301 TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA
  361 GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC
  421 GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTCCC
  481 ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG
  541 GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT
  601 ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT TACTATGGGA ACATACGTCA
  661 TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA
  721 GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT
  781 ATTAATAACT AATGCATGGC GGTAATACGG TTATCCACGC GGCCGCAATA AAATATCTTT
  841 ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT
  901 CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GGCTGTCCCC CAGTGCCAAG
  961 TGCAGGGTGC CAGAACAATT TTCTCCTAAT CCGATAGGGT ACCCTGGATA ATCAAAGTGG
 1021 GGCTTCCCTA CTAGATCATG CCAGGGTCCA CCCAAATACC CAGTTTAATC AGGATGAAGA
1081 AGGAAATGTA CAGGTAATAA AAAAGAGAGG TGTGAACA
//
Primer: IRES-R
Sequence file: RDB13967_A6Btb.seq check
>D03116A1_A6Bt_1_IRES_R_B05_05.ab1
    1 GGAACCCCGG CTTGGCCAGT AACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGATAT
   61 CTTACAGCCA GGTTAGAAAG GGCTCACTTT TGTTCAGAGC AGACTCTACA CCATTGAAGA
  121 GGGGGATCAG GTCTTCAGAT TCCAAAGTTC CTAAGTCAAC GTTTGTTCCT GGAAGACAGT
  181 CAAGGAAATC AGGGTAACGG GTCTGTTGGG GATTGATGTT CATGGGTGTT TGCCCTGCGT
  241 TTTCTCCTGT ATCCATCTCA TCCACATTGC TGAGGAAGTC CTCCGGAGTT GTGGGGACAC
  301 TGTAGCACCC TAACCCCAGG CCACTGTCAG TGCTCTGCTC CCTCGAATGA TATGGCCCTC
  361 CATTGAGGAA AGGATCTGAG CTATTATTAG TGATGGATCT CATGTCTGGG GTCATCGTGG
  421 GTGGGTTGAC AGCAGCCTGA ACTGGGGCAA GAGTCTCAGC TTCCATGGGG AGCTGTCGAC
  481 AGAGGGCAGC TTCCTGCCTC ATGAGCTCCT CTTGGCGCAT TCGAATCCTT TCTCTCTCCA
  541 TCTGGATTCT CTGAAGCCGC AGTTTCTGCT GCTGCTGCTG CTGAGTGGTC AGCGCATTGG
  601 GCATACTCAT GAGCCCTGCG GGTGGGTTCT GAGTGGGGTG GTTCTGCTGG CTCAGGGTAC
  661 TGGGGGCCAT CTGCTGCTGG TGTTGGTGAT TCATCACGAG ATTTGGCTGG GATACTGCCA
  721 TGGACCTCTG AGGCACTGGT GTGGAACTGA CGGCAGGGTG GAGGTTCATA TGATTCAGAG
  781 GCTGATTCAT CGCCTTCCTA GGGTCTTGCC ATGTGGTGAT TTTTTCTATG TGGTTGAGGA
  841 AGTACCTCTG GCCAGTGGCC GTGAAGGTCA TCTCCCAGCC CGGGGGCAGT GGCAGCTCGT
  901 CGGTCACGTC GTAGGACTGC TGGCGGAGGT GCGCGTGCTG CTGCGCGGGG CTACCCGCAG
  961 CACCCGCGCC GGTGCCCAGC TGCAGGGACG CGGGGCGACG AGTGCGAGCG GACATGCTGG
 1021 GCACCCCCCA GCCCAGTCGA GGCCCCGGGG TGGCCGCCCG ACGAAGTCGG GTGCCTGGAC
 1081 TGCGCGGAGT GCGAAGCCCG AATCCAGCTT CTAAGAAGAC TTCGGCAAGA ATTCTTCCTT
1141 CCGCCCAACC GAAGCCTTAT AGGC
//
Primer: Red-N_R
Sequence file: RDB13967_A6Btc.seq check
>D03116A1_A6Bt_1_IRES-F_B03_06.ab1
    1 TCCGACCGTT ATATGTGGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
   61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
  121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCAATTC
  841 GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG
  901 TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC
  961 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA
 1021 TACGCTGCTT TAATGCCCTT TGTATCATGC TATTGCTTTC CCGTATGGCT TTCATTTTTT
 1081 CTCCTCGTTG TAATAAATCC TGATTGGCTG TTCTCTTATG AGGAAGTTGT GGCCGATGTC
1141 AGGCCAACGT TGGCCGTTGA TTTC
//
Primer: cPPT+CTS_v2_F
Sequence file: RDB13967_A6Btd.seq check
>D03116A1_A6Bt_1_Red_N_R_D05_11.ab1
    1 GTTACCATGG GGGCACTGGA AGCGCATGAA CTCCTTGATG ATGGCCATGT TATCCTCCTC
   61 GCCCTTGCTC ACCATGGTGG CGACCGGTGG ATCCTTAGAG CTAGTGTACT TGGTAACTGC
  121 CTTAGTGCCC TCGGACACAG CATGCTTAGC CAGCTCCCCA GGCAGCAGCA GGCGCACAGC
  181 CGTCTGAATC TCCCTGGAGG TGATGGTCGA GCGCTTATTG TAGTGAGCCA GGCGAGAAGC
  241 CTCGCCCGCG ATGCGCTCGA AGATGTCGTT GACGAAGGAG TTCATGATCC CCATGGCCTT
  301 GGATGAGATG CCGGTGTCGG GGTGGACCTG CTTCAGAACC TTGTACACAT AGATAGAATA
  361 GCTCTCCTTG CGGCTGCGCT TACGCTTCTT ACCATCCTTC TTCTGCGCCT TAGTGATAGC
  421 CTTCTTAGAA CCCTTTTTAG GGGCTGGAGC AGACTTAGAG GGTTCAGGCA TGGTGGCGGT
  481 ACCAAGCTTG GAAGAGAGTG AGGACGAACG CGCCCCCACC CCCTTTTATA GCCCAGATCT
  541 CGAGCCCGGG CTAGCACGCG TGTCTGCTAG GTCTGCACCG AGTTGCTGTG TGGGACTTGT
  601 CTGTTCTGCA ATACCTTTTA TATCCACTGC CTGAGCCCCT AATGTAAGCA GGAGGGGCAG
  661 GAGATGCTGC CAGGACTAAT TGGGGGTCAT GTGCGCAATA GGTCAAACCC AAAGGATGCT
  721 GTGGTTGGTG ACTGCAGCTG CCGGGGCGAA GGCCTGGAAG GGGAGGGTAG GACACAATCA
  781 GAGGCTGCCG GGTCCTGACA TGGCCCCAGA CCTCTTCCCG GAGGGGCTGC TCCAGCACCC
  841 CTACTGTGTG CCATCTCTCT CAGCCTCAGC TGGAGCACTG GCTGGCCCTA GAGACGAGCT
  901 CCTTAGAGCA TAGCCTAGAG AGGGTACAGA GATCTAAAAT CGGTTTGGGA CAAACTGGCT
  961 CCAACTCGCA TAGCCTGTGA TTTTCAGTGT CTGCCGTGAG AGCAGGAGCC AAACCCCCAG
 1021 GAAGGAGCGG TCAGTAATGT CCCTCATTCA CCCAGTGCAA GGGCAGCTTC CGCCTCATCC
 1081 AGTGGGGAGA AGGGTGGGGG AAAGTGTCTG CCTAGTTTTC TGGTCACTTG GAAGAAGAAA
1141 AGCCCTTCAA TTGGAGG
//
Primer: HIVgp_R1
Sequence file: RDB13967_A6Bte.seq check
>D03116A1_A6Bt_1_cPPT+CTS_v2_F_C05_08.ab1
    1 ACGTTGACAT CCGAAACTAA AGAATTACAA AAAACAATTA CAAAAATTCA AAATTTTCGG
   61 GTTTATTACA GGGACAGCAG AGATCCAGTT TGGTTAGTAC CGGGCCCGCT CTAGATTACT
  121 TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA
  181 CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC
  241 CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC
  301 CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT
  361 ACATCCGCTC GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG
  421 AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG
  481 AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA
  541 AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG
  601 CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC
  661 CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC
  721 CCTCAATCTC GAAACTCGTG GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG
  781 AACTCCTTGA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT
  841 GGATCCTTAG AGCTAGTGTA CTTGGTAACT GCCCTTAGTG CCCTCGGACA CAGCATGCTT
  901 AGCCAGCTCC CCAAGGCAGC AGCAGGCGCA CAGCCGTCTG AATCTCCCCT GGAAGGTGAT
  961 GGTCGAGCGG CTTATTGTAA TGAGCCAGCG AGAAAGCCTC GCCCGCCGAT GCCGCCTCGA
 1021 AGATGTCATT TGACGAAAGG GAATTTCAAT GAACTCCCCC ATTGGGCCCT TTGGAATGAA
 1081 AATGTGCCCA GGATGTTCCG GGGGGGTGGC ACACTTGGCC TCTCCACAAC GCTTGGTAAC
 1141 AGCATATGAA TGGAAAATAA GGCATCCTCC CTATTAGTCA GGCGCTTGTC GCACTCGCTT
1201 GATACGCG
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB13967_A6Btf.seq check
>D03116A1_A6Bt_1_HIVgp_R1_E05_14.ab1
    1 CCGGCCATCT TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCTTGG
   61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC
  121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC
  181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT
  241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG
  301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC
  361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC
  421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG
  481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC
  541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG
  601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA
  661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT
  721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG
  781 CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC
  841 TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATAATAG ACCTCCCCAC
  901 CGTACACGGC CTACCGCCCA TTTTGCGTCA ATGGGGCGGA GTTGTTACGA CATTTTTGGA
  961 AAAGTCCCGT TTGATTTTGG TGCCAAACAA ACTCCCATTG ACGTCAATGG GGGGTGGAAG
 1021 ACTTGGGAAA TCCCCGTGAG TCCAAACCGC TATCCCACGG CCAATTGATG TACTGCCAAA
 1081 AACCGGCATC ACCATGTAAA TAGCGAATGA CTAAATACGT AGATGTACTG GCAAGTTAGG
1141 AAAGTCCCAT TAGGTCAATG TATACCTGGG GCA
//
Primer:
Sequence file: RDB13967_A6Btg.seq check
>D03116A1_A6Bt_1_SV40pA3'Element_reverse_G05_20.ab1
    1 GTGCAAGGGG CGGACCTATG GAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
   61 TTGCTGGCCT TTTGCTCACA TGCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG
  121 GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG
  181 CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA
  241 GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG
  301 AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC
  361 ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT
  421 CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC
  481 TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG
  541 ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT
  601 TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG
  661 TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT
  721 GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GATCGAGGTC
  781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA AGGGAGATCC GACTCGTCTG AGGGCGAAAG
  841 CGAAGACGCG GAAGAGGCCG CAGAGCCCGG CAGCAGCCCG CGGGAAGGAA GGTCCGCTGG
  901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGATCCAGTG GCACACAGGC
  961 GAGCAGCCAG AAGAACGATG ATTTCCCCGA CACACACGAT GTCAGTGCCA CCAGCGAGCC
 1021 CTGTCAGCAG CGGGCAGCAA GCGGCATGAG TTCGGCGTGG CCAAAGAGGA GCGAAAGTCC
1081 GGCAGGACTG ACAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]