Please review the QC test results indicated by icon below as well as clone information before placing your order.

pLL3.7 K122 FH-TAZ-ires-GFP-TEAD-responsive-H2B mCherry

A system that monitors directly the TAZ-dependent transcription.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link] Schedule A (GFP-A) [Link]
Remarks	Remember that you will be working with samples containing infectious virus. An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose). Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link] Schedule A（GFP-A, 英語版のみ） [Link]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。 本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA（Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用）をご参照ください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13967_A6Btp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: hTAZ-N_R Sequence file: RDB13967_A6Bta.seq
>D03116A1_A6Bt_1_hTAZ-N_R_F12_18.ab1     1 CGAGAGGCTC GGAGGTCTGT GTCTAGGTCC TGCGTGACGT GGATCACTTG CTGCCCAGGC    61 GGCGGGAGCG GAGGGGGCGC CGAGGCCGGA TTCATCTTCT GGGCGGGCAG CGAAGCTGAG   121 CCTGAGCGCG TGAATTCATG ATGATGATGA TGGTGCAATT CCTTGTCGTC ATCGTCTTTG   181 TAGTCCATGG CCCCGGCTAG CGGATCTGAC GGTTCACTAA ACCAGCTCTG CTTATATAGA   241 CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG GGCGGAGTTG TTACGACATT   301 TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA   361 GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC   421 GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTCCC   481 ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG   541 GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT   601 ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT TACTATGGGA ACATACGTCA   661 TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA   721 GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT   781 ATTAATAACT AATGCATGGC GGTAATACGG TTATCCACGC GGCCGCAATA AAATATCTTT   841 ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT   901 CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GGCTGTCCCC CAGTGCCAAG   961 TGCAGGGTGC CAGAACAATT TTCTCCTAAT CCGATAGGGT ACCCTGGATA ATCAAAGTGG  1021 GGCTTCCCTA CTAGATCATG CCAGGGTCCA CCCAAATACC CAGTTTAATC AGGATGAAGA 1081 AGGAAATGTA CAGGTAATAA AAAAGAGAGG TGTGAACA //
Primer: IRES-R Sequence file: RDB13967_A6Btb.seq
>D03116A1_A6Bt_1_IRES_R_B05_05.ab1     1 GGAACCCCGG CTTGGCCAGT AACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGATAT    61 CTTACAGCCA GGTTAGAAAG GGCTCACTTT TGTTCAGAGC AGACTCTACA CCATTGAAGA   121 GGGGGATCAG GTCTTCAGAT TCCAAAGTTC CTAAGTCAAC GTTTGTTCCT GGAAGACAGT   181 CAAGGAAATC AGGGTAACGG GTCTGTTGGG GATTGATGTT CATGGGTGTT TGCCCTGCGT   241 TTTCTCCTGT ATCCATCTCA TCCACATTGC TGAGGAAGTC CTCCGGAGTT GTGGGGACAC   301 TGTAGCACCC TAACCCCAGG CCACTGTCAG TGCTCTGCTC CCTCGAATGA TATGGCCCTC   361 CATTGAGGAA AGGATCTGAG CTATTATTAG TGATGGATCT CATGTCTGGG GTCATCGTGG   421 GTGGGTTGAC AGCAGCCTGA ACTGGGGCAA GAGTCTCAGC TTCCATGGGG AGCTGTCGAC   481 AGAGGGCAGC TTCCTGCCTC ATGAGCTCCT CTTGGCGCAT TCGAATCCTT TCTCTCTCCA   541 TCTGGATTCT CTGAAGCCGC AGTTTCTGCT GCTGCTGCTG CTGAGTGGTC AGCGCATTGG   601 GCATACTCAT GAGCCCTGCG GGTGGGTTCT GAGTGGGGTG GTTCTGCTGG CTCAGGGTAC   661 TGGGGGCCAT CTGCTGCTGG TGTTGGTGAT TCATCACGAG ATTTGGCTGG GATACTGCCA   721 TGGACCTCTG AGGCACTGGT GTGGAACTGA CGGCAGGGTG GAGGTTCATA TGATTCAGAG   781 GCTGATTCAT CGCCTTCCTA GGGTCTTGCC ATGTGGTGAT TTTTTCTATG TGGTTGAGGA   841 AGTACCTCTG GCCAGTGGCC GTGAAGGTCA TCTCCCAGCC CGGGGGCAGT GGCAGCTCGT   901 CGGTCACGTC GTAGGACTGC TGGCGGAGGT GCGCGTGCTG CTGCGCGGGG CTACCCGCAG   961 CACCCGCGCC GGTGCCCAGC TGCAGGGACG CGGGGCGACG AGTGCGAGCG GACATGCTGG  1021 GCACCCCCCA GCCCAGTCGA GGCCCCGGGG TGGCCGCCCG ACGAAGTCGG GTGCCTGGAC  1081 TGCGCGGAGT GCGAAGCCCG AATCCAGCTT CTAAGAAGAC TTCGGCAAGA ATTCTTCCTT 1141 CCGCCCAACC GAAGCCTTAT AGGC //
Primer: Red-N_R Sequence file: RDB13967_A6Btc.seq
>D03116A1_A6Bt_1_IRES-F_B03_06.ab1     1 TCCGACCGTT ATATGTGGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG    61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG   121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC   181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC   241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC   301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC   421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC   481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC   541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG   601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG   661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC   721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC   781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCAATTC   841 GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG   901 TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC   961 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA  1021 TACGCTGCTT TAATGCCCTT TGTATCATGC TATTGCTTTC CCGTATGGCT TTCATTTTTT  1081 CTCCTCGTTG TAATAAATCC TGATTGGCTG TTCTCTTATG AGGAAGTTGT GGCCGATGTC 1141 AGGCCAACGT TGGCCGTTGA TTTC //
Primer: cPPT+CTS_v2_F Sequence file: RDB13967_A6Btd.seq
>D03116A1_A6Bt_1_Red_N_R_D05_11.ab1     1 GTTACCATGG GGGCACTGGA AGCGCATGAA CTCCTTGATG ATGGCCATGT TATCCTCCTC    61 GCCCTTGCTC ACCATGGTGG CGACCGGTGG ATCCTTAGAG CTAGTGTACT TGGTAACTGC   121 CTTAGTGCCC TCGGACACAG CATGCTTAGC CAGCTCCCCA GGCAGCAGCA GGCGCACAGC   181 CGTCTGAATC TCCCTGGAGG TGATGGTCGA GCGCTTATTG TAGTGAGCCA GGCGAGAAGC   241 CTCGCCCGCG ATGCGCTCGA AGATGTCGTT GACGAAGGAG TTCATGATCC CCATGGCCTT   301 GGATGAGATG CCGGTGTCGG GGTGGACCTG CTTCAGAACC TTGTACACAT AGATAGAATA   361 GCTCTCCTTG CGGCTGCGCT TACGCTTCTT ACCATCCTTC TTCTGCGCCT TAGTGATAGC   421 CTTCTTAGAA CCCTTTTTAG GGGCTGGAGC AGACTTAGAG GGTTCAGGCA TGGTGGCGGT   481 ACCAAGCTTG GAAGAGAGTG AGGACGAACG CGCCCCCACC CCCTTTTATA GCCCAGATCT   541 CGAGCCCGGG CTAGCACGCG TGTCTGCTAG GTCTGCACCG AGTTGCTGTG TGGGACTTGT   601 CTGTTCTGCA ATACCTTTTA TATCCACTGC CTGAGCCCCT AATGTAAGCA GGAGGGGCAG   661 GAGATGCTGC CAGGACTAAT TGGGGGTCAT GTGCGCAATA GGTCAAACCC AAAGGATGCT   721 GTGGTTGGTG ACTGCAGCTG CCGGGGCGAA GGCCTGGAAG GGGAGGGTAG GACACAATCA   781 GAGGCTGCCG GGTCCTGACA TGGCCCCAGA CCTCTTCCCG GAGGGGCTGC TCCAGCACCC   841 CTACTGTGTG CCATCTCTCT CAGCCTCAGC TGGAGCACTG GCTGGCCCTA GAGACGAGCT   901 CCTTAGAGCA TAGCCTAGAG AGGGTACAGA GATCTAAAAT CGGTTTGGGA CAAACTGGCT   961 CCAACTCGCA TAGCCTGTGA TTTTCAGTGT CTGCCGTGAG AGCAGGAGCC AAACCCCCAG  1021 GAAGGAGCGG TCAGTAATGT CCCTCATTCA CCCAGTGCAA GGGCAGCTTC CGCCTCATCC  1081 AGTGGGGAGA AGGGTGGGGG AAAGTGTCTG CCTAGTTTTC TGGTCACTTG GAAGAAGAAA 1141 AGCCCTTCAA TTGGAGG //
Primer: HIVgp_R1 Sequence file: RDB13967_A6Bte.seq
>D03116A1_A6Bt_1_cPPT+CTS_v2_F_C05_08.ab1     1 ACGTTGACAT CCGAAACTAA AGAATTACAA AAAACAATTA CAAAAATTCA AAATTTTCGG    61 GTTTATTACA GGGACAGCAG AGATCCAGTT TGGTTAGTAC CGGGCCCGCT CTAGATTACT   121 TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA   181 CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC   241 CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC   301 CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT   361 ACATCCGCTC GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG   421 AGGGGAAGTT GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG   481 AGTCCTGGGT CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA   541 AGCCCTCGGG GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG   601 CCTTGGAGCC GTACATGAAC TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCAC   661 CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC   721 CCTCAATCTC GAAACTCGTG GCCGTTCACG GAGCCCTCCA TGTGCACCTT GAAGCGCATG   781 AACTCCTTGA TGATGGCCAT GTTATCCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT   841 GGATCCTTAG AGCTAGTGTA CTTGGTAACT GCCCTTAGTG CCCTCGGACA CAGCATGCTT   901 AGCCAGCTCC CCAAGGCAGC AGCAGGCGCA CAGCCGTCTG AATCTCCCCT GGAAGGTGAT   961 GGTCGAGCGG CTTATTGTAA TGAGCCAGCG AGAAAGCCTC GCCCGCCGAT GCCGCCTCGA  1021 AGATGTCATT TGACGAAAGG GAATTTCAAT GAACTCCCCC ATTGGGCCCT TTGGAATGAA  1081 AATGTGCCCA GGATGTTCCG GGGGGGTGGC ACACTTGGCC TCTCCACAAC GCTTGGTAAC  1141 AGCATATGAA TGGAAAATAA GGCATCCTCC CTATTAGTCA GGCGCTTGTC GCACTCGCTT 1201 GATACGCG //
Primer: SV40pA3'Element Reverse Sequence file: RDB13967_A6Btf.seq
>D03116A1_A6Bt_1_HIVgp_R1_E05_14.ab1     1 CCGGCCATCT TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCTTGG    61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC   121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC   181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT   241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG   301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC   361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC   421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG   481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC   541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG   601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA   661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT   721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG   781 CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC   841 TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATAATAG ACCTCCCCAC   901 CGTACACGGC CTACCGCCCA TTTTGCGTCA ATGGGGCGGA GTTGTTACGA CATTTTTGGA   961 AAAGTCCCGT TTGATTTTGG TGCCAAACAA ACTCCCATTG ACGTCAATGG GGGGTGGAAG  1021 ACTTGGGAAA TCCCCGTGAG TCCAAACCGC TATCCCACGG CCAATTGATG TACTGCCAAA  1081 AACCGGCATC ACCATGTAAA TAGCGAATGA CTAAATACGT AGATGTACTG GCAAGTTAGG 1141 AAAGTCCCAT TAGGTCAATG TATACCTGGG GCA //
Primer: Sequence file: RDB13967_A6Btg.seq
>D03116A1_A6Bt_1_SV40pA3'Element_reverse_G05_20.ab1     1 GTGCAAGGGG CGGACCTATG GAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT    61 TTGCTGGCCT TTTGCTCACA TGCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG   121 GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG   181 CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA   241 GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG   301 AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC   361 ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT   421 CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC   481 TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG   541 ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT   601 TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG   661 TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT   721 GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GATCGAGGTC   781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA AGGGAGATCC GACTCGTCTG AGGGCGAAAG   841 CGAAGACGCG GAAGAGGCCG CAGAGCCCGG CAGCAGCCCG CGGGAAGGAA GGTCCGCTGG   901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGATCCAGTG GCACACAGGC   961 GAGCAGCCAG AAGAACGATG ATTTCCCCGA CACACACGAT GTCAGTGCCA CCAGCGAGCC  1021 CTGTCAGCAG CGGGCAGCAA GCGGCATGAG TTCGGCGTGG CCAAAGAGGA GCGAAAGTCC 1081 GGCAGGACTG ACAGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl