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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST131NGFP126 (#RDB13952)

Expression vector of human CSAP, GFP tag.

Clone info. Expression vector of human CSAP, GFP tag.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 7.8 kb
Selectable markers Ampicillin^r (E. coli), Neomycin^r (mammalian cell)
Gene/insert name Human CSAP cDNA
Depositor|Developer Ohta, Shinya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Ohta, S., PLos One 10 (11): e0142798 2015.)

Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohta, S., PLos One 10 (11): e0142798 2015.)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13952 pDEST131NGFP126 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST131NGFP126 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13952).

Reference section:

Ohta, S., Hamada, M., Sato, N., Toramoto, I., Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles. PLoS One 10 (11): e0142798, 2015. PMID 26562023. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13952_A6Bip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13952_A6Bia.seq check
>D03129A1_A6Bi_2_CMV_Forward_A10_01.ab1
    1 TTGACACTTC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACG ATGGTGAGCA
  181 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  241 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  301 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  361 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  421 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  481 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  541 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  601 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  661 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  721 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  781 CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  841 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT ACCACAAGTT
  901 TGTACAAAAA AGCACGCTCC ACCATGGGAA CCATGTCCCC GGGGAGCGGG GTGAAGAGCG
  961 AGTACATGAA GCGCTACCAG GAGCCGCGCT GGGAGGGAGT ACGGGCCCGT GCTACCGCGA
 1021 GCTGCTGCAC TACCGCCTAG CGCCGGGCTG CTGGGAGCAG CGCACGCCGC CTGGCTCTGG
1081 GACGACTGGA GCCGCTGCTT CTTCGAGACT CGGCGATCGA TCAGAAGTTC GGT
//
Primer: EGFP-C
Sequence file: RDB13952_A6Bib.seq check
>D03129A1_A6Bi_2_EGFP_C_B10_04.ab1
    1 ACGGTACTCT CGGCATGGAC GAGCTGTACA AGGGTACCAC AAGTTTGTAC AAAAAAGCAG
   61 GCTCCACCAT GGGAACCATG TCCCCGGGGA GCGGGGTGAA GAGCGAGTAC ATGAAGCGCT
  121 ACCAGGAGCC GCGCTGGGAG GAGTACGGGC CGTGCTACCG CGAGCTGCTG CACTACCGCC
  181 TAGGCCGCCG GCTGCTGGAG CAGGCGCACG CGCCCTGGCT CTGGGACGAC TGGGGCCCGG
  241 CCGGCTCCTC GGAGGACTCG GCGTCGTCAG AGTCGTCGGG CGCCGGGGGC CCCGCACCCC
  301 GGTGCGCCCC GCCCTCGCCC CCGCCGCCCG TAGAGCCGGC GACCCAGGAG GAGGCGGAAC
  361 GGCGGGCGCG CGGGGCCCCG GAGGAGCAGG ACGCGGAGGC CGGGGACGCG GAGGCCGAGG
  421 ACGCGGAGGA CGCGGCTCTG CCAGCACTGC CAGTGAAAGA TGTAGAAGAT AAACCTGAAC
  481 AACAAACCAG AACAAGAGAG ACTGACAAAT CACCCACCAG TACTGAGCCT CGACAGCAAC
  541 CAAGTGCCTT ATTTGCTAGA GGAAACAGGA AAGCGGTCAA AAGTCCCCAA AGATCATCGA
  601 GTAAAATAAA AGAAAACAAG CATCCATTTG CTCTTTATGG CTGGGGAGAA AAACAGACCG
  661 ATACAGGAAG CCAGAAGACT CACAACGTCT GTGCGTCCGC TCCTGTGCAC GAGATTCATG
  721 AATCAGCATT ACGAGCCAAG AACAGAAGAC AGGTGGAAAA AAGGAAACTG GTTGCTCAAA
  781 GGCAGCGAGC TCACTCTGTG GATGTGGAGA AGAACAGAAA GATGAAGGCT TCCTCCTCAG
  841 AGAACCCATG GATGACAGAA TACATGAGGT GCTATTCGGC AAGAGCTTAT CTAGACCCAG
  901 CTTTCTTGTA CAAAGTGGTC TAAAGTAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC
  961 CTCGACTGTG CCCTTCTAGT TGCCCAGCCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
 1021 CTTGACCCTG GAAGTGCCAC CTCCCACTGT CCTTTCCTAT TAAATTGAAG AATGCATCGC
1081 ATTGTCTGAC TAGTGTCAAT TCTATTTCTG CGGGTGGGGG TTGGGGGCCA A
//
Primer: pAxEF_R2
Sequence file: RDB13952_A6Bic.seq check
>D03129A1_A6Bi_2_pAxEF_R2_D10_10.ab1
    1 ATGGTCAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTGGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG
  121 ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG
  181 GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC
  241 AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG
  301 GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG
  361 CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC
  421 AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC
  481 CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCGCGC CTTGAGCCTG GCGAACAGTT
  541 CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT
  601 CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG
  661 CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG
  721 GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC
  781 TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC
  841 ACGATAGCCG CGCTGCCTCG TCCTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA
  901 CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA
  961 TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCCTCTCCA CCCAAGCGGC CCGGGAGAAC
 1021 CTGCGTGCAA TCCATCTGTT CATCATGCGA AACGATCCTT CATCTGTCTC TGAATCAGAT
1081 TCCGAAATGT ATATACAGCT CCCGGGAAGC TTTTTGCAAA AGGCCTAGGC CCTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, S., Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles. PLoS One 10 (11): e0142798, 2015. PMID 26562023. [link to RRC of NBRP]