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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

FLAG-His-eIF5A on pcDNA3.1 (#RDB13751)

Expression vector of human eIF5A.

Clone info. Expression vector of FLAG- and His-tagged human eIF5A.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pcDNA3.1 Directional TOPO (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name human eIF5A cDNA
Depositor|Developer Seko, Yoshinori |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Seko, Y., Sci. Rep. 5: 13737 2015.)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Seko, Y., Sci. Rep. 5: 13737 2015.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13751 FLAG-His-eIF5A on pcDNA3.1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The FLAG-His-eIF5A on pcDNA3.1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13751).

Reference section:

Seko, Y., Fujimura, T., Yao, T., Taka, H., Mineki, R., Okumura, K., Murayama, K., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13751_A6A5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13751_A6A5a.seq check
>D02955A2_A6A5_6_CMV_Forward_A09_03.ab1
    1 ATGTTTCACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT TGGTACCGAG CTCGGATCCA
  181 GTACCCTTCA CCGAATTCAA AATGGCAGAT GACTTGGACT TCGAGACAGG AGATGCAGGG
  241 GCCTCAGCCA CCTTCCCAAT GCAGTGCTCA GCATTACGTA AGAATGGCTT TGTGGTGCTC
  301 AAAGGCCGGC CATGTAAGAT CGTCGAGATG TCTACTTCGA AGACTGGCAA GCACGGCCAC
  361 GCCAAGGTCC ATCTGGTTGG TATTGACATC TTTACTGGGA AGAAATATGA AGATATCTGC
  421 CCGTCAACTC ATAATATGGA TGTCCCCAAC ATCAAAAGGA ATGACTTCCA GCTGATTGGC
  481 ATCCAGGATG GGTACCTATC ACTGCTCCAG GACAGCGGGG AGGTACGAGA GGACCTTCGT
  541 CTCCCTGAGG GAGACCTTGG CAAGGAGATT GAGCAGAAGT ACGACTGTGG AGAAGAGATC
  601 CTGATCACGG TGCTGTCTGC CATGACAGAG GAGGCAGCTG TTGCAATCAA GGCCATGGCA
  661 AAAGATTACA AGGACGACGA TGACAAGCAC CACCATCACC ATCACTGACT CGAGTATATA
  721 AGGGTCAAGA CAATTCTGCA GATATCCAGC ACAGTGGCGG CCGCTCGAGT CTAGAGGGCC
  781 CGCGGTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG
  841 GTCATCATCA CCATCACCAT TGAGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA
  901 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA
  961 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAAGGTGT
 1021 CATTCTATTT CTGGGGGGGG GTGGGGGTGG GGGCAGGACA GCACGGGGGG AGGATTGGGG
 1081 AAGAACCAAT TAGCAGCCAT GGCCTGGGGG ATGGCGGTGG GGGCTTCTTA TGTGGGCCTT
1141 TCTCTTGAAG
//
Primer: SV40pro_F
Sequence file: RDB13751_A6A5b.seq check
>D02955A2_A6A5_6_SV40pro_F_C09_09.ab1
    1 CCTGTCTTGC ATTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC CAAGCGACGC
  961 CCAACCCTGC CATCACGAGA TTTCGATTCC CACCGCCCGC CCTTTCTATG AAAGTTGGGC
 1021 TTTCGGAATC GTTTTCCGGG GACGCCGGGC TGGGATGATC TTCAGCGCCG GGGATCTCAT
1081 GCTGGAGTTC TTCGCCAACC CCGACCTGCT TTAATTGCAG CATTATAATG GG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594. [link to RRC of NBRP]