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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

FLAG-His-eIF5A on pcDNA3.1

Expression vector of human eIF5A.

Catalog number RDB13751
Resource name FLAG-His-eIF5A on pcDNA3.1
Clone info. Expression vector of FLAG- and His-tagged human eIF5A.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pcDNA3.1 Directional TOPO (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name human eIF5A cDNA
Depositor|Developer Seko, Yoshinori |
Other clones in our bank
External Database
human eIF5A
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Seko, Y., Sci. Rep. 5: 13737 2015.)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Seko, Y., Sci. Rep. 5: 13737 2015.)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB13751 FLAG-His-eIF5A on pcDNA3.1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13751_A6A5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13751_A6A5a.seq check
          >D02955A2_A6A5_6_CMV_Forward_A09_03.ab1
              1 ATGTTTCACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT TGGTACCGAG CTCGGATCCA
            181 GTACCCTTCA CCGAATTCAA AATGGCAGAT GACTTGGACT TCGAGACAGG AGATGCAGGG
            241 GCCTCAGCCA CCTTCCCAAT GCAGTGCTCA GCATTACGTA AGAATGGCTT TGTGGTGCTC
            301 AAAGGCCGGC CATGTAAGAT CGTCGAGATG TCTACTTCGA AGACTGGCAA GCACGGCCAC
            361 GCCAAGGTCC ATCTGGTTGG TATTGACATC TTTACTGGGA AGAAATATGA AGATATCTGC
            421 CCGTCAACTC ATAATATGGA TGTCCCCAAC ATCAAAAGGA ATGACTTCCA GCTGATTGGC
            481 ATCCAGGATG GGTACCTATC ACTGCTCCAG GACAGCGGGG AGGTACGAGA GGACCTTCGT
            541 CTCCCTGAGG GAGACCTTGG CAAGGAGATT GAGCAGAAGT ACGACTGTGG AGAAGAGATC
            601 CTGATCACGG TGCTGTCTGC CATGACAGAG GAGGCAGCTG TTGCAATCAA GGCCATGGCA
            661 AAAGATTACA AGGACGACGA TGACAAGCAC CACCATCACC ATCACTGACT CGAGTATATA
            721 AGGGTCAAGA CAATTCTGCA GATATCCAGC ACAGTGGCGG CCGCTCGAGT CTAGAGGGCC
            781 CGCGGTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG
            841 GTCATCATCA CCATCACCAT TGAGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA
            901 GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA
            961 CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAAGGTGT
           1021 CATTCTATTT CTGGGGGGGG GTGGGGGTGG GGGCAGGACA GCACGGGGGG AGGATTGGGG
           1081 AAGAACCAAT TAGCAGCCAT GGCCTGGGGG ATGGCGGTGG GGGCTTCTTA TGTGGGCCTT
          1141 TCTCTTGAAG
          //
          Primer: SV40pro_F
          Sequence file: RDB13751_A6A5b.seq check
          >D02955A2_A6A5_6_SV40pro_F_C09_09.ab1
              1 CCTGTCTTGC ATTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
             61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
            121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
            661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
            781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
            841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
            901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC CAAGCGACGC
            961 CCAACCCTGC CATCACGAGA TTTCGATTCC CACCGCCCGC CCTTTCTATG AAAGTTGGGC
           1021 TTTCGGAATC GTTTTCCGGG GACGCCGGGC TGGGATGATC TTCAGCGCCG GGGATCTCAT
          1081 GCTGGAGTTC TTCGCCAACC CCGACCTGCT TTAATTGCAG CATTATAATG GG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl