RDB13504 - pET-His UNAG

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icon below as well as clone information before placing your order.

pET-His UNAG (#RDB13504)

Expression clone of eel UnaG in E.coli.

Clone info.	Expression clone of eel (Anguilla japonica) UnaG in E.coli.
Vector backbone	pET32a (+) (Plasmid)
Size of vector backbone	5.9 kb
Selectable markers	Amp^r
Growth conditions	LB + Amp, 37oC
Gene/insert name	eel UnaG cDNA
Depositor\|Developer	Taketani, Shigeru \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Takeda T.A., Sci. Rep. 5, 10488 (2015)).
Additional terms and conditions:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Takeda T.A., Sci. Rep. 5, 10488 (2015)). Additional terms and conditions: 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takeda T.A., Sci. Rep. 5, 10488 (2015))。付加的使用条件： 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takeda T.A., Sci. Rep. 5, 10488 (2015))。
付加的使用条件：
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13504	pET-His UNAG	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-His UNAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13504).

Reference section:

Takeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13504_A7Ctp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F (Pr0191) Region: lacO,RBS,TrxA,6XHis,thrombin site,S-tag,MCS,UnaG Sequence file: RDB13504_A7Ctd.seq
>13504_13504_A7Ct_3_pET-check-F_F04_16_ABI24.ab1 1 GGGGGTCGCA CGTCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC 61 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA 121 GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG 181 ATCGATCTCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC 241 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGAGCGATA 301 AAATTATTCA CCTGACTGAC GACAGTTTTG ACACGGATGT ACTCAAAGCG GACGGGGCGA 361 TCCTCGTCGA TTTCTGGGCA GAGTGGTGCG GTCCGTGCAA AATGATCGCC CCGATTCTGG 421 ATGAAATCGC TGACGAATAT CAGGGCAAAC TGACCGTTGC AAAACTGAAC ATCGATCAAA 481 ACCCTGGCAC TGCGCCGAAA TATGGCATCC GTGGTATCCC GACTCTGCTG CTGTTCAAAA 541 ACGGTGAAGT GGCGGCAACC AAAGTGGGTG CACTGTCTAA AGGTCAGTTG AAAGAGTTCC 601 TCGACGCTAA CCTGGCCGGT TCTGGTTCTG GCCATATGCA CCATCATCAT CATCATTCTT 661 CTGGTCTGGT GCCACGCGGT TCTGGTATGA AAGAAACCGC TGCTGCTAAA TTCGAACGCC 721 AGCACATGGA CAGCCCAGAT CTGGGTACCG ACGACGACGA CAAGGCCATG GCTGATATCG 781 GATCCGGTGG TTCTGGTATG GGTCGAGAAA TTTGTTGGCA CCTGGAAGAT TCGCAGACAG 841 TCCATTAATT TTGGTTGAAT ACCC //
Primer: f1ori_R3 (Pr0142) Region: T7 terminator,MCS,UnaG,enterokinase site,S-tag,thrombin site,6XHis,TrxA Sequence file: RDB13504_A7Cte.seq
>13504_13504_A7Ct_3_f1ori_R3_A05_02_ABI24.ab1 1 GGCAATGGCA TCGGATTAGT TCCTCCTTTC AGCAAAAACC CCTCAAGACC CGTTTAGAGG 61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC 121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA 181 GCTTGTCGAC GGAGCTCGAA TTCTCATTCC GTCGCCCTCC GGTAGCTGCG CACAGCCACG 241 ACGTCTCCCA TCGTAAGTGT CACGACCAGT TTACCGTCCT TTATCTCTCG GACATACGTC 301 GTCTCCTTGC CGTCCCACTT TTGTACGTAC ACCAGCTTCT CTCCCACCAA GTTCACGACA 361 GATTTTACGC CTTTTCTTCG ATCAGAAGGA AATTCGTCGA ACTCCTCCCC TAATTTGAAC 421 TTTACTTGAG TGTCAAGGAA CGTAGGAGGT CCATTCTCTA TTTTCACTGT CATTTTGTCT 481 CCGTCCTTCT GGGAGATGTA CAATGTCGGC GTCGTGGCAT CCCCACCATC GCTTAATTCC 541 TTTGGGGCTC CGATAGCTTT CAGGTATTCA CCAAAATTAT GGCTGTCTGC GATCTTCCAG 601 GTGCCAACAA ATTTCTCGAC CATACCAGAA CCACCGGATC CGATATCAGC CATGGCCTTG 661 TCGTCGTCGT CGGTACCCAG ATCTGGGCTG TCCATGTGCT GGCGTTCGAA TTTAGCAGCA 721 GCGGTTTCTT TCATACCAGA ACCGCGTGGC ACCAGACCAG AAGAATGATG ATGATGATGG 781 TGCATATGGC CAGAACCAGA ACCGGCCAGG TTAGCGTCGA GGAACTCTTT CAACTGACCT 841 TTAGACAGTG CACCCACTTT GGTTGCCGCC ACTTCACCGT TTTTGAACAG CAGCAGAGTC 901 GGGATACCAC GGATGCCATA TTTCGGCGCA GTGCCAGGGG TTTTGATCGA TGTTCAGTTT 961 GCAACGGGTC AGTTTGCCCT GATATTCGTC AGCGATTTCA TCCAGAATCG GGGCGATCAT 1021 TTTTGCACGG ACCGCACCAC CTCTGCCCAG AAATTCGAAC GAAGAATCGG CCCCCGATCC 1081 GCATTTGAAG TACATCGATG TCCAAAACCT GATCGGTTCG AGTCAAGGGT GA //
Primer: pGL3_RV4 (Pr0335) Region: lacI Sequence file: RDB13504_A7Ctf.seq
>13504_13504_A7Ct_3_pGL3_Rv4_D05_11_ABI24.ab1 1 CTCAACGATG GGTTGATGCT CTCAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG 61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA 181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC 241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT 301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA 361 CCGAGATGTC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG 421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA 481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA 541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC 601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC 661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA 721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT 781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG 841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA 901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGCGCGTGCA GGGCCAGACT 961 GGAGTGGCAC GCCCATCAGC CACCGACTGT TGCCGCCAGT GTGTGCCACG CCATGGGATG 1021 TATTCAGCTT CGGCCATCGC GGCATCCACT TTTCTGCGAT TTCGCAGAAT CGTGGCTTGG 1081 CCGTTTGGAT TCAC //
Primer: lacIpro_F (Pr0409) Region: lacI Sequence file: RDB13504_A7Ctg.seq
>13504_13504_B0BaF_lacIpro_F_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNN NNNNNNNGNN NNGNNNNNAA TGNNNNNACC 61 AGTAACGTTA TACGATGNNN NNNNNAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC 121 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG 181 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG 241 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG 301 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC 361 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG 421 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT 481 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTNNNNNN 541 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC 601 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT 661 AAATATNNCN NNCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC 721 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG 781 CTGGTTGCCN NCGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACNNA GTCCNGNCTG 841 CGCGTTGGTG NGGACNTCTC GNNNNNGGNA NNNNANNANA CCGAAANANN GCTCATGTTA 901 TNTCCCGCCG TTANCCNCNA TCNAANNAGG ANTTTNNCCT GCTGGGGGCA ANNCNNNNNG 961 NACCGNNTNG CTGCAACTCT CTCNNGNNCN NNNNNNGAAN GGNCNNTCAN CNGNNGNCCN 1021 NNNTCANTGN NGAAAANNAA AAANCNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takeda, T.A., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [link to RRC of NBRP]
reference	Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
reference	Hieu Liem, P., A simple and highly sensitive method of measuring heme oxygenase activity. Biol. Chem. 396 (11): 1265-1268 (2015). PMID 26040009. [link to RRC of NBRP]
reference	Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
review	Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

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External Database
eel UnaG

Reference sequence