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pET-His UNAG (#RDB13504)

Expression clone of eel UnaG in E.coli.

Clone info. Expression clone of eel (Anguilla japonica) UnaG in E.coli.
Vector backbone pET32a (+) (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name eel UnaG cDNA
Depositor|Developer Taketani, Shigeru |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Takeda T.A., Sci. Rep. 5, 10488 (2015)).
Additional terms and conditions:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takeda T.A., Sci. Rep. 5, 10488 (2015))。
付加的使用条件：
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13504 pET-His UNAG DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-His UNAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13504).

Reference section:

Takeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13504_A7Ctp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F (Pr0191)
Region: lacO,RBS,TrxA,6XHis,thrombin site,S-tag,MCS,UnaG
Sequence file: RDB13504_A7Ctd.seq check
>13504_13504_A7Ct_3_pET-check-F_F04_16_ABI24.ab1
    1 GGGGGTCGCA CGTCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC
   61 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA
  121 GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG
  181 ATCGATCTCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC
  241 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGAGCGATA
  301 AAATTATTCA CCTGACTGAC GACAGTTTTG ACACGGATGT ACTCAAAGCG GACGGGGCGA
  361 TCCTCGTCGA TTTCTGGGCA GAGTGGTGCG GTCCGTGCAA AATGATCGCC CCGATTCTGG
  421 ATGAAATCGC TGACGAATAT CAGGGCAAAC TGACCGTTGC AAAACTGAAC ATCGATCAAA
  481 ACCCTGGCAC TGCGCCGAAA TATGGCATCC GTGGTATCCC GACTCTGCTG CTGTTCAAAA
  541 ACGGTGAAGT GGCGGCAACC AAAGTGGGTG CACTGTCTAA AGGTCAGTTG AAAGAGTTCC
  601 TCGACGCTAA CCTGGCCGGT TCTGGTTCTG GCCATATGCA CCATCATCAT CATCATTCTT
  661 CTGGTCTGGT GCCACGCGGT TCTGGTATGA AAGAAACCGC TGCTGCTAAA TTCGAACGCC
  721 AGCACATGGA CAGCCCAGAT CTGGGTACCG ACGACGACGA CAAGGCCATG GCTGATATCG
  781 GATCCGGTGG TTCTGGTATG GGTCGAGAAA TTTGTTGGCA CCTGGAAGAT TCGCAGACAG
841 TCCATTAATT TTGGTTGAAT ACCC
//
Primer: f1ori_R3 (Pr0142)
Region: T7 terminator,MCS,UnaG,enterokinase site,S-tag,thrombin site,6XHis,TrxA
Sequence file: RDB13504_A7Cte.seq check
>13504_13504_A7Ct_3_f1ori_R3_A05_02_ABI24.ab1
    1 GGCAATGGCA TCGGATTAGT TCCTCCTTTC AGCAAAAACC CCTCAAGACC CGTTTAGAGG
   61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
  121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA
  181 GCTTGTCGAC GGAGCTCGAA TTCTCATTCC GTCGCCCTCC GGTAGCTGCG CACAGCCACG
  241 ACGTCTCCCA TCGTAAGTGT CACGACCAGT TTACCGTCCT TTATCTCTCG GACATACGTC
  301 GTCTCCTTGC CGTCCCACTT TTGTACGTAC ACCAGCTTCT CTCCCACCAA GTTCACGACA
  361 GATTTTACGC CTTTTCTTCG ATCAGAAGGA AATTCGTCGA ACTCCTCCCC TAATTTGAAC
  421 TTTACTTGAG TGTCAAGGAA CGTAGGAGGT CCATTCTCTA TTTTCACTGT CATTTTGTCT
  481 CCGTCCTTCT GGGAGATGTA CAATGTCGGC GTCGTGGCAT CCCCACCATC GCTTAATTCC
  541 TTTGGGGCTC CGATAGCTTT CAGGTATTCA CCAAAATTAT GGCTGTCTGC GATCTTCCAG
  601 GTGCCAACAA ATTTCTCGAC CATACCAGAA CCACCGGATC CGATATCAGC CATGGCCTTG
  661 TCGTCGTCGT CGGTACCCAG ATCTGGGCTG TCCATGTGCT GGCGTTCGAA TTTAGCAGCA
  721 GCGGTTTCTT TCATACCAGA ACCGCGTGGC ACCAGACCAG AAGAATGATG ATGATGATGG
  781 TGCATATGGC CAGAACCAGA ACCGGCCAGG TTAGCGTCGA GGAACTCTTT CAACTGACCT
  841 TTAGACAGTG CACCCACTTT GGTTGCCGCC ACTTCACCGT TTTTGAACAG CAGCAGAGTC
  901 GGGATACCAC GGATGCCATA TTTCGGCGCA GTGCCAGGGG TTTTGATCGA TGTTCAGTTT
  961 GCAACGGGTC AGTTTGCCCT GATATTCGTC AGCGATTTCA TCCAGAATCG GGGCGATCAT
 1021 TTTTGCACGG ACCGCACCAC CTCTGCCCAG AAATTCGAAC GAAGAATCGG CCCCCGATCC
1081 GCATTTGAAG TACATCGATG TCCAAAACCT GATCGGTTCG AGTCAAGGGT GA
//
Primer: pGL3_RV4 (Pr0335)
Region: lacI
Sequence file: RDB13504_A7Ctf.seq check
>13504_13504_A7Ct_3_pGL3_Rv4_D05_11_ABI24.ab1
    1 CTCAACGATG GGTTGATGCT CTCAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG
   61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
  121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
  181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
  241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
  301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
  361 CCGAGATGTC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
  421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
  481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
  541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
  601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC
  661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA
  721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT
  781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG
  841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA
  901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGCGCGTGCA GGGCCAGACT
  961 GGAGTGGCAC GCCCATCAGC CACCGACTGT TGCCGCCAGT GTGTGCCACG CCATGGGATG
 1021 TATTCAGCTT CGGCCATCGC GGCATCCACT TTTCTGCGAT TTCGCAGAAT CGTGGCTTGG
1081 CCGTTTGGAT TCAC
//
Primer: lacIpro_F (Pr0409)
Region: lacI
Sequence file: RDB13504_A7Ctg.seq check
>13504_13504_B0BaF_lacIpro_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNN NNNNNNNGNN NNGNNNNNAA TGNNNNNACC
   61 AGTAACGTTA TACGATGNNN NNNNNAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC
  121 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG
  181 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG
  241 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG
  301 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC
  361 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG
  421 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT
  481 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTNNNNNN
  541 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC
  601 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT
  661 AAATATNNCN NNCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC
  721 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG
  781 CTGGTTGCCN NCGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACNNA GTCCNGNCTG
  841 CGCGTTGGTG NGGACNTCTC GNNNNNGGNA NNNNANNANA CCGAAANANN GCTCATGTTA
  901 TNTCCCGCCG TTANCCNCNA TCNAANNAGG ANTTTNNCCT GCTGGGGGCA ANNCNNNNNG
  961 NACCGNNTNG CTGCAACTCT CTCNNGNNCN NNNNNNGAAN GGNCNNTCAN CNGNNGNCCN
1021 NNNTCANTGN NGAAAANNAA AAANCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takeda, T.A., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [link to RRC of NBRP]
reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
reference Hieu Liem, P., A simple and highly sensitive method of measuring heme oxygenase activity. Biol. Chem. 396 (11): 1265-1268 (2015). PMID 26040009. [link to RRC of NBRP]
reference Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.