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pET-His UNAG (#RDB13504)

Expression clone of eel UnaG in E.coli.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Takeda, T.A., Sci. Rep. 5: 10488 (2015). PMID 25990790. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression clone of eel (Anguilla japonica) UnaG in E.coli.
Vector backbone pET32a (+) (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name eel UnaG cDNA
Depositor|Developer Taketani, Shigeru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Takeda T.A., Sci. Rep. 5, 10488 (2015)).
Additional terms and conditions:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takeda T.A., Sci. Rep. 5, 10488 (2015))。
付加的使用条件：
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Kumagai, A. et al., Cell 153 (7): 1602-1611 (2013).

Catalog # Resource name Shipping form Fee
RDB13504 pET-His UNAG DNA solution

How to cite this biological resource

Materials & Methods section:

The pET-His UNAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13504).

Reference section:

Takeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takeda, T.A., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [PubMed] [Article] [RRC of NBRP]
reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682. [PubMed] [Article]
reference Hieu Liem, P., A simple and highly sensitive method of measuring heme oxygenase activity. Biol. Chem. 396 (11): 1265-1268 (2015). PMID 26040009. [PubMed] [Article] [RRC of NBRP]
reference Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [PubMed] [Article] [RRC of NBRP]
review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623. [PubMed] [Article]