RDB13486 - pCAG-GFP-mTrf1 cDNA

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icon below as well as clone information before placing your order.

pCAG-GFP-mTrf1 cDNA (#RDB13486)

Visualization of telomeres. Expression vector of mouse Trf1 in mammalian cells.

Clone info.	Expression vector of mouse Trf1 in mammalian cells. Visualization of telomeres.
Comment	Refer Proc Natl Acad Sci U S A. 101 (1) :16-22 (2004) as a reference.
Vector backbone	pCAGGS (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Trf1 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Watanabe, Yoshinori \| Shibuya, Hiroki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13486	pCAG-GFP-mTrf1 cDNA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-GFP-mTrf1 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13486).

Reference section:

Shibuya, H., Ishiguro, K., Watanabe, Y., The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat. Cell Biol. 16 (2): 145-156 (2014). PMID 24413433. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13486_A6Dqp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB13486_A6Dqa.seq
>D02365A1_A6Dq_3_pAxCA_F1_B02_05.ab1 1 CTTGGTACGT TGCTCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC 61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCGC CACCATGGTG AGCAAGGGCG 121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 241 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA 301 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA 421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC 481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT 541 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT 601 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA 661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT 721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA 781 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GCTCGAGATG GCGGAGACGG 841 TCTCCTCAGC GGCCCGGGAC GCGCCGAGCC GTGAGGGCTG GACAGATTCG GATTCTCCAG 901 AGCAGGAGGA GGTGGGAGAC GACGCGGAGC TGCTCCAGTG CCAGCTTTCA GCTGGGGGAC 961 CCCGAGAGAG ATGGGAGAAC GCGGGAGCTT GTGGCTGAGT GGAGGCCGTG GGCTGCGGCT 1021 GGATGCTCGA CTTCCTCTGG CTGGTCTCTG ATGCCGAGGT CCGTTCCCGC CGAACGGGGC //
Primer: EGFP-C Sequence file: RDB13486_A6Dqb.seq
>D02365A1_A6Dq_3_EGFP_C_C02_08.ab1 1 GCAGGTGTCT CGGGCATGGA CGAGCTGTAC AGCTCGAGAT GGCGGAGACG GTCTCCTCAG 61 CGGCCCGGGA CGCGCCGAGC CGTGAGGGCT GGACAGATTC GGATTCTCCA GAGCAGGAGG 121 AGGTGGGAGA CGACGCGGAG CTGCTCCAGT GCCAGCTTCA GCTGGGGACC CCGAGAGAGA 181 TGGAGAACGC GGAGCTTGTG GCTGAGGTGG AGGCCGTGGC TGCGGGCTGG ATGCTCGACT 241 TCCTCTGCCT GTCTCTGTGC CGAGCCTTCC GCGACGGCCG CTCCGAGGAC TTTCGTCGTA 301 CTCGTGACAG CGCCGAGGCT ATTATTCATG GACTACACAG ACTTACAGCT TACCAATTGA 361 AAACTGTGTA TATATGTCAG TTTTTGACAA GAGTTGCATC TGGAAAGGCC CTTGATGCAC 421 AGTTTGAAGT TGATGAGCGT ATTACACCCT TGGAATCAGC CCTGATGATT TGGAACTCAA 481 TTGAAAAGGA ACATGACAAA CTGCATGACG AAATAAAGAA TTTAATTAAA ATTCAGGCTG 541 TAGCTGTTTG TATGGAAATT GGCAGCTTTA AGGAAGCAGA AGAAGTATTT GAAAGAATAT 601 TTGGTGATCC AGAATTTTAC ACGCCTTTAG AAAGGAAGTT ACTTAAGATA ATCTCTCAGA 661 AGGATGTGTT CCACTCCCTT TTCCAACACT TCAGCTATAG CTGCATGATG GAGAAAATTC 721 AGAGTTATGT GGGTGATGTG TTAAGTGAAA AATCATCAAC TTTTCTAATG AAGGCAGCAA 781 CAAAAGTAGT GGAAAATGAG AAAGCGAGGA CACAAGCGTC TAAGGATAGG CCAGATGCCA 841 CCAACACTGG AATGGACACT GAAGTTGGTT TGAATAAAGA GAAAAGTGTT AATGGCCAGC 901 AGTCTACAGA AACTGAAACC CTTAGTGGAT ACAGTATCCC TCAAATAATG TCTCACAAGA 961 ACGCCCTTTA TCGCAGTTTA AAACACAGAC GTGCTCCATT CAGAATTTCC AGGTAGCACG 1021 GAAAGCAAGG AACAGCAACT CTTCCAGCTG TGAATCATCG ATGTAAGGGA TCGGAAGAAA 1081 CCAG //
Primer: pAxCA_R1 Sequence file: RDB13486_A6Dqc.seq
>D02365A1_A6Dq_3_pAxCA_R1_D02_11.ab1 1 GTGGGGAAGG TGGCATTGGC ACACCAGGCC ACCACCTTCG TGATAGGCAG CCTGCACCTG 61 AGGAGTGCGG CCGCTCAGCT AATCAGTTTC AGTCTCTTCA TTGTTCTCCA TCTATCTTTT 121 AACATGACAC TTGTTCGGTT GTTGAACTTA TAATGGGATA GTATTTTAGC CCAATTTCCC 181 TCTCCATATT TCTTTACACC ACACTTCAAA ATTCTGTCTT CTTCCCAAAG CCATGTCTGT 241 CTTTTCCTGC GTCCACTTTT GTCATCAGTG TCTGGCTGAT TCTCTGAGAC ACACAATCTA 301 TTCCTTCCAC TGGTTCTTCG GTTCCTTTCC ATCGTTGTTT CACACTGAAG AGTTCCTGTT 361 CTTGCTTCGT TCCTACTGAA ATCTGATGGA GCACGTCTGT GTTTTAACTG CGATAAGGCG 421 TTCTTGTGAG ACCTTATTGA GGATACTGTA TCCACTAAGG GTTCAGTTTC TGTAGACTGC 481 TGGCCATTAA CACTTTTCTC TTTATTCAAA CCAACTTCAG TGTCCATTCC AGTGTTGGTG 541 GCATCTGGCC TATCCTTAGA CGCTTGTGTC CTCGCTTTCT CATTTTCCAC TACTTTTGTT 601 GCTGCCTTCA TTAGAAAAGT TGATGATTTT TCACTTAACA CATCACCCAC ATAACTCTGA 661 ATTTTCTCCA TCATGCAGCT ATAGCTGAAG TGTTGGAAAA GGGAGTGGAA CACATCCTTC 721 TGAGAGATTA TCTTAAGTAA CTTCCTTTCT AAAGGCGTGT AAAATTCTGG ATCACCAAAT 781 ATTCTTTCAA ATACTTCTTC TGCTTCCTTA AAGCTGCCAA TTTCCATACA AACAGCTACA 841 GCCTGAATTT TAATTAAATT CTTTATTTCG TCATGCAGTT TGTCATGTTC CTTTTCAATT 901 GAGTTCCAAA TCATCACGGC TGATTCCAAA GGGTGTAATA CGCTCATCAA CTTCAAACTG 961 TGCATCAACG GGCCCTTTCC AGATGCAAAC TCTTTGTCAA AAAACTGACA TATTATACAC 1021 AGTTTTCAAT TTGGTAAAGC TGTAAGTCCT GTGTAGTTCC AATGGATTAA TAGCTCGACG 1081 CTGTCACGAA GTACGAACGG AAAAAGGATC CCTTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shibuya, H., The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat. Cell Biol. 16 (2): 145-156 (2014). PMID 24413433. [link to RRC of NBRP]