Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-BOSneo-Myc-MINT deltaN-NLS

Expression vector of mouse SPEN/MINT cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources. Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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提供条件

本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(Kuroda, K., Immunity, 18, 301-312, 2003)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

提供依頼

提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13474_A5Hep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 () Sequence file: RDB13474_A5Hea.seq
>D02692A3_A5He_4_pAxEF_F1_B02_05_ABI24.ab1     1 GATTTTTTTC TTCATTTCAG GTGTCGTGAG GAATTCTCTA GCCTCGACGG TATCGATTTA    61 AAGCTATGGA GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA   121 TTTCTGAAGA GGACTTGAAT GAAATGGAGC AAAAGCTCAT TTCTGAAGAG GACTTGAATG   181 AAATGGAGCA AAAGCTCATT TCTGAAGAGG ACTTGAATGA AATGGAGCAA AAGCTCATTT   241 CTGAAGAGGA CTTGAATGAA ATGGAGAGCT TGGGCGACCT CACCATGGAG CAAAAGCTCA   301 TTTCTGAAGA GGACTTGAAT TTGCCGAAAA AGAAGAGAAA GGTGGAATTC ATGAAGAAGA   361 AGAAAATCAG GACGGACTCG GAGGGGAAAC TGGCTGATAA AAAGGACGAG CGCAGGGAGG   421 AGGAGCAGGA GAGGCAGGAG TTGTTCGCCT CCCGGTTCTT ACACAGCTCC ATCTTTGAGC   481 AAGACTCCAA GAGGCTGCAG CACCTTGAGA GGAAGAGCGA GGAGCCCGAC CTCCCTCCCG   541 GGAGGCTGTA CGGCAGGCAG GCGCCTGAGG GTGCAAACAG CACGAGTGAT TCGGTGCAGG   601 AGCCAGTGGT CCTGTTCCAC AGCAGATTCC TGGAGCTGAC GCGGATGCAG CAGAAGGAGA   661 AGGAGAAGGA CCAGAAGCCC AAAGAGGCTG AGAAGCAGGA AGAGCCGGAG ACTCATCCCA   721 AGACTCCAGA GCCTGCTGCA GAGACTAAAG AGCCGGAGCC GAAAGCCCCT GTTTCAGCTG   781 GGCTTCCTGC CGTCACCATC ACCGTCGTGA CACCAGAGCC AGCATCCTCC GCCCCTGAGA   841 AAGCTGAGGA GGCAGCGGAG GCTCCTTCAC CAGCAGGAGA GAAGCCTGCA GAGCCCGCTC   901 CTGTCTCGGA GAAAACAAAG CTTGTGTCTG AGCCTGCGTC TGTACCCGTG GGAGCAGCCC   961 AGGCAGTCAG ATGTACCTCC CCGGAGAGGA CAGCCCGGGA TAGCCAAGGA CAGCGCTTGC  1021 TTGCATCTTC AGCTCTCAGA AAGCCGCAGC TACTGGACGC GTCCTTGGTG TGATGCTGAA 1081 CTTGACTCCG AACCTCAGTT CCAGGTAGAA AGAGCC //
Primer: hGCSF_R () Sequence file: RDB13474_A5Heb.seq
>D02692A3_A5He_4_hGCSF_R_A02_02_ABI24.ab1     1 CGTGGGGAAA TGATCTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGGA TATCATCTAG    61 AACTAGTGGA TCCTTTTTTT TTTTTTTTGC AGCAGAGCCT CAGGAAAATG CCCTGAGGTG   121 GCGACCGTTC AGAGCCTCAC ACCGAGGCAA TGACAATCAT GAGGTGAGGA GAGATGTTGG   181 AGATGCTGGC AAGGAGGTCA GGGGCCAGCC GGGAGAGGTG ACTCTCGGAG AACTCACAGG   241 GTGGGAAGAT CTGAAGCACG TAGGCAGGCT GATTGGAGCC AGGGTTGGGG ACATTGATAA   301 TCCCTGCTGC TTGCTTGGCC TGCAGGTAAG TGATGAAGGC AGCCTTGAGG GACTCGGTCT   361 GGCTCACCAC ATCCTCCTGG TCACGGCCAC AGGGCAGAGC CAGCAGCAGG CAGTAGTCGG   421 TTTCCACTGT CATTCTCCTG GCGACCCCTT CCAGCTGTGA GGCCTCTAGT CGCATCCTCT   481 GGGCGATCCG CAGTGGGGGA CCACCTTCAG AGAGAGGCAG GGACCGATGA GCCAGGACAT   541 TGTTGCCAGA GACGAAGTGG AGCTGCACAG CAGCCGTGTC GTTCTTGAGG GCCAGCAGGC   601 CCTGCCACAC GATGGGGTAC TTCTTCAGCA GCTGGACCAT ATCCACAGGC CGTTGTGGTG   661 TCCCGTGTGG AGAAGACTCT TGTTTGGGAG CTGGCTGGAC TTCAGCGTGA GGCAGAGCCA   721 GTCCAGGTGG GGTGCTTGGG CCTGAGTTTG CAGGGACAAA CAATGGCTGC TTTGGGGCAG   781 CCTGAGAGGA AAGAGGGCTT GGGAGGTCTG GCTTCATGGA GACGGACACA GGGGAGGGGT   841 GGGCTGGCTG GGCTGTTTCC ACCGGCGCCC TCTGAAGCTG AGCGTGTGGG CTCAGATGGC   901 CTGTTTGTGG GGGATGGCTG GGGAGGCGAG TCTGCTCCCA CCCCTCCTGT CTGCGTCCCC   961 TTTGCTTCTT GGGAGACCTG AGGAAATCTT GCCACTCGGT GGCTGGGCAG GCCTGCTGAG  1021 TTGGAAGGCT GGCAAGCCGG CAAGTTGTAT AGCTGCTGGC TGCGTAAAGG GGGCAAGCCT 1081 GTTGCCCAGA ACG //
Primer: HSV_TK_pA_R () Sequence file: RDB13474_A5Hec.seq
>D02692A3_A5He_4_HSV_TK_pA_R_C02_08_ABI24.ab1     1 CAAAAATCGG TCAGAAGGCG ATAGAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT    61 AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG   121 CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG   181 AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA   241 GATCCTCGCC GTCGGGCATG CGCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC   301 CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG   361 CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT   421 GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG   481 ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA   541 CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG   601 CCTCGTCCTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC   661 GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC   721 AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT   781 GTTCAATGGC CGATCCCATA TTGGCTGCAG GGTCGCTCGG TGTTCGAGGC CACACGCGTC   841 ACCTTAATAT GCGAAGTGGA CCTGGGACCG CGCCGCCCCC GACTGCATCT GCGTGTTCGA   901 ATTCGCCAAT GACAAGACGC TGGGGCGGGG TTTGCTCGAC ATTGGGTGGA AACATTCCAG   961 GCCTGGTGGG AGAGGCTTTT TTGCTTCCCT CTTGCAAAAC CACCACTGCT CGACATTTGG  1021 GTTGAAACAT TCCAGGCCTG GGTGGAGAGC TTTTTTGCTT CCTCCTGAAA TACACTGCTT 1081 CGAACTGGCC GAAAGGGGGG ATTGTTGCCT TGACA //
Primer: EF1a-N_R () Sequence file: RDB13474_A5Hed.seq
>D02692A3_A5He_4_EF1a-N_R_E02_14_ABI24.ab1     1 AATCCGGGGG GGTAATGTGG GCGATGTGCG CTCTGCCCAC TGACGGGCAC CGGAGCCAAT    61 TCCCACTCCT TTCAAGACCT AGAAGGTCCA TTAGCTGCAA AGATTCCTCT CTGTTTAAAA   121 CTTTATCCAT CTTTGCAAAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC   181 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT   241 AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA   301 GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT   361 GCTGGGGAGG GAATTAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG   421 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG   481 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC   541 GGGAAACCTG TCGTGCCAGT CGAGAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG   601 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG   661 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC   721 GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA   781 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG   841 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA   901 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC   961 GCGAGACGAA AGGGCCTTCG TGATAACGCC TATTTTTTAT AGGGTTAATG TTCAATGAAT  1021 AATAAATGGG TTTTCCTTTA GAACGTTCAA GGTGCCACTT TTCGGGGGGA AATGGTGCGG 1081 CGGACCCTTA TTTGGTTTAA TTTTTCCTAA AATACCAATT TTCCAAAATA AA //
Primer: MINT-RAM7_F () Sequence file: RDB13474_A5Hee.seq
>D02692A3_A5He_4_MINT-RAM7_F_D02_11_ABI24.ab1     1 GATCTACCAT CTCCAGGCCC TTCCCCCAGA CACAAAGGCC TCTGATATGG ACACCAGCTC    61 CAGTACACTG AGGAAGATCC TCATGGACCC CAAATATGTG TCTGCCACAG GAGTTACGTC   121 CACAAGTGTC ACTTCAGCCA TTGCAGAGCC CGTGAGTGCG CCTTGTCTGC AGGAGGCGCC   181 GGCCCCTCCC TGTGACCCTA AGCATCCTCC CTTAGAAGGC GTGTCAGCAG CCGCAGTACC   241 AAATGCTGAC ACACAAGCCT CAGAGGTTCC GGTGGCTGCC GACAAAGAGA AGGTGGCTCC   301 GGTCATTGCC CCTAAAATCA CGTCGGTTAT TAGCCGGATG CCCGTTAGCA TTGACCTGGA   361 GAACTCGCAG AAGATCACGT TGGCAAAGCC AGCTCCTCAG ACCCTGACTG GCCTGGTGAG   421 TGCCCTGACT GGTCTGGTGA ATGTCTCTCT GGTCCCGGTG AATGCCTTAA AGGGCCCTGT   481 GAAGGGCTCA GTAGCCACCC TGAAAGGTTT GGTGAGCACC CCTGCTGGGC CCGTGAATCT   541 CCTAAAGGGG CCCGTGAATG TCCTCACGGG GCCTGTCAAT GTCCTGACTA CTCCTGTGAG   601 TGCCACAGTG GGCACGGTGA ACGCTGCCCC CGGCCCCGTG ACTGCTGCCT GTGGCGTGAC   661 GGCCACAACA GGCACAGCAG CCGTGACGGG TGCAGTGACT GCCCCAGCAG CCAAAGGGCA   721 AGCAGCGGGC CAGTAGCAAT GAAGAACAGC CGCTTTCCAC CCTGGGTCCA TGTCAGTGAT   781 TGATGACGAC CAGCAGACAC GGACTTCTGG CCGCGGGGCT GCGGTGAATA CATCAGAAGG   841 GGTCGTGCTG CTGAGCTACT CGGGACAGAA AAACGGGAAG GCTCCAGCGA TCAGTGCTAG   901 ATCAGCCAGA TCCTCCAGCA GCGCATGGGA CATCGAGTCA AGCAGGTCAG ATCCAGGTTC   961 CGAGTGAAAG CGCGATCGCA TACACTCTGA ATCGATTCGC ACTAAGGCTG AACTAAGCTT 1021 GCAAGTGGGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

GNP_filter3_RDBDEP_html_231001.pl