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pEF-BOSneo-Myc-MINT deltaN-NLS (#RDB13474)

Expression vector of mouse SPEN/MINT cDNA

Clone info. Expression vector of mouse SPEN/MINT cDNA. deltaN-NLS in Figure 1A of Kuroda, K. et al., Immunity 18 (2), 301-312, 2003.
Comment Known difference (at least): Nucleotide sequences of vector-insert junction and variable region were checked and found at least six nucleotide substitutions comparing with NM_019763.2 (cds, 352..11283), G to A at 5074nt (Gly to Arg), T to C at 5095nt (Ser to Pro), A to C at 5161nt (Met to Leu), G to A at 5329nt (Val to Ile), T to C at 4983nt (silent), and C to T at 5067nt (silent).
Vector backbone pEF-BOS Neo (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Mint cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(Kuroda, K., Immunity, 18, 301-312, 2003)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13474 pEF-BOSneo-Myc-MINT deltaN-NLS DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneo-Myc-MINT deltaN-NLS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13474).

Reference section:

Kuroda, K., Han, H., Tani, S., Tanigaki, K., Tun, T., Furukawa, T., Taniguchi, Y., Kurooka, H., Hamada, Y., Toyokuni, S., Honjo, T., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13474_A5Hep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 ()
Sequence file: RDB13474_A5Hea.seq check
>D02692A3_A5He_4_pAxEF_F1_B02_05_ABI24.ab1
    1 GATTTTTTTC TTCATTTCAG GTGTCGTGAG GAATTCTCTA GCCTCGACGG TATCGATTTA
   61 AAGCTATGGA GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG CAAAAGCTCA
  121 TTTCTGAAGA GGACTTGAAT GAAATGGAGC AAAAGCTCAT TTCTGAAGAG GACTTGAATG
  181 AAATGGAGCA AAAGCTCATT TCTGAAGAGG ACTTGAATGA AATGGAGCAA AAGCTCATTT
  241 CTGAAGAGGA CTTGAATGAA ATGGAGAGCT TGGGCGACCT CACCATGGAG CAAAAGCTCA
  301 TTTCTGAAGA GGACTTGAAT TTGCCGAAAA AGAAGAGAAA GGTGGAATTC ATGAAGAAGA
  361 AGAAAATCAG GACGGACTCG GAGGGGAAAC TGGCTGATAA AAAGGACGAG CGCAGGGAGG
  421 AGGAGCAGGA GAGGCAGGAG TTGTTCGCCT CCCGGTTCTT ACACAGCTCC ATCTTTGAGC
  481 AAGACTCCAA GAGGCTGCAG CACCTTGAGA GGAAGAGCGA GGAGCCCGAC CTCCCTCCCG
  541 GGAGGCTGTA CGGCAGGCAG GCGCCTGAGG GTGCAAACAG CACGAGTGAT TCGGTGCAGG
  601 AGCCAGTGGT CCTGTTCCAC AGCAGATTCC TGGAGCTGAC GCGGATGCAG CAGAAGGAGA
  661 AGGAGAAGGA CCAGAAGCCC AAAGAGGCTG AGAAGCAGGA AGAGCCGGAG ACTCATCCCA
  721 AGACTCCAGA GCCTGCTGCA GAGACTAAAG AGCCGGAGCC GAAAGCCCCT GTTTCAGCTG
  781 GGCTTCCTGC CGTCACCATC ACCGTCGTGA CACCAGAGCC AGCATCCTCC GCCCCTGAGA
  841 AAGCTGAGGA GGCAGCGGAG GCTCCTTCAC CAGCAGGAGA GAAGCCTGCA GAGCCCGCTC
  901 CTGTCTCGGA GAAAACAAAG CTTGTGTCTG AGCCTGCGTC TGTACCCGTG GGAGCAGCCC
  961 AGGCAGTCAG ATGTACCTCC CCGGAGAGGA CAGCCCGGGA TAGCCAAGGA CAGCGCTTGC
 1021 TTGCATCTTC AGCTCTCAGA AAGCCGCAGC TACTGGACGC GTCCTTGGTG TGATGCTGAA
1081 CTTGACTCCG AACCTCAGTT CCAGGTAGAA AGAGCC
//
Primer: hGCSF_R ()
Sequence file: RDB13474_A5Heb.seq check
>D02692A3_A5He_4_hGCSF_R_A02_02_ABI24.ab1
    1 CGTGGGGAAA TGATCTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGGA TATCATCTAG
   61 AACTAGTGGA TCCTTTTTTT TTTTTTTTGC AGCAGAGCCT CAGGAAAATG CCCTGAGGTG
  121 GCGACCGTTC AGAGCCTCAC ACCGAGGCAA TGACAATCAT GAGGTGAGGA GAGATGTTGG
  181 AGATGCTGGC AAGGAGGTCA GGGGCCAGCC GGGAGAGGTG ACTCTCGGAG AACTCACAGG
  241 GTGGGAAGAT CTGAAGCACG TAGGCAGGCT GATTGGAGCC AGGGTTGGGG ACATTGATAA
  301 TCCCTGCTGC TTGCTTGGCC TGCAGGTAAG TGATGAAGGC AGCCTTGAGG GACTCGGTCT
  361 GGCTCACCAC ATCCTCCTGG TCACGGCCAC AGGGCAGAGC CAGCAGCAGG CAGTAGTCGG
  421 TTTCCACTGT CATTCTCCTG GCGACCCCTT CCAGCTGTGA GGCCTCTAGT CGCATCCTCT
  481 GGGCGATCCG CAGTGGGGGA CCACCTTCAG AGAGAGGCAG GGACCGATGA GCCAGGACAT
  541 TGTTGCCAGA GACGAAGTGG AGCTGCACAG CAGCCGTGTC GTTCTTGAGG GCCAGCAGGC
  601 CCTGCCACAC GATGGGGTAC TTCTTCAGCA GCTGGACCAT ATCCACAGGC CGTTGTGGTG
  661 TCCCGTGTGG AGAAGACTCT TGTTTGGGAG CTGGCTGGAC TTCAGCGTGA GGCAGAGCCA
  721 GTCCAGGTGG GGTGCTTGGG CCTGAGTTTG CAGGGACAAA CAATGGCTGC TTTGGGGCAG
  781 CCTGAGAGGA AAGAGGGCTT GGGAGGTCTG GCTTCATGGA GACGGACACA GGGGAGGGGT
  841 GGGCTGGCTG GGCTGTTTCC ACCGGCGCCC TCTGAAGCTG AGCGTGTGGG CTCAGATGGC
  901 CTGTTTGTGG GGGATGGCTG GGGAGGCGAG TCTGCTCCCA CCCCTCCTGT CTGCGTCCCC
  961 TTTGCTTCTT GGGAGACCTG AGGAAATCTT GCCACTCGGT GGCTGGGCAG GCCTGCTGAG
 1021 TTGGAAGGCT GGCAAGCCGG CAAGTTGTAT AGCTGCTGGC TGCGTAAAGG GGGCAAGCCT
1081 GTTGCCCAGA ACG
//
Primer: HSV_TK_pA_R ()
Sequence file: RDB13474_A5Hec.seq check
>D02692A3_A5He_4_HSV_TK_pA_R_C02_08_ABI24.ab1
    1 CAAAAATCGG TCAGAAGGCG ATAGAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT
   61 AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG
  121 CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG
  181 AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA
  241 GATCCTCGCC GTCGGGCATG CGCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC
  301 CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG
  361 CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT
  421 GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG
  481 ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA
  541 CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG
  601 CCTCGTCCTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC
  661 GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC
  721 AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT
  781 GTTCAATGGC CGATCCCATA TTGGCTGCAG GGTCGCTCGG TGTTCGAGGC CACACGCGTC
  841 ACCTTAATAT GCGAAGTGGA CCTGGGACCG CGCCGCCCCC GACTGCATCT GCGTGTTCGA
  901 ATTCGCCAAT GACAAGACGC TGGGGCGGGG TTTGCTCGAC ATTGGGTGGA AACATTCCAG
  961 GCCTGGTGGG AGAGGCTTTT TTGCTTCCCT CTTGCAAAAC CACCACTGCT CGACATTTGG
 1021 GTTGAAACAT TCCAGGCCTG GGTGGAGAGC TTTTTTGCTT CCTCCTGAAA TACACTGCTT
1081 CGAACTGGCC GAAAGGGGGG ATTGTTGCCT TGACA
//
Primer: EF1a-N_R ()
Sequence file: RDB13474_A5Hed.seq check
>D02692A3_A5He_4_EF1a-N_R_E02_14_ABI24.ab1
    1 AATCCGGGGG GGTAATGTGG GCGATGTGCG CTCTGCCCAC TGACGGGCAC CGGAGCCAAT
   61 TCCCACTCCT TTCAAGACCT AGAAGGTCCA TTAGCTGCAA AGATTCCTCT CTGTTTAAAA
  121 CTTTATCCAT CTTTGCAAAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC
  181 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT
  241 AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA
  301 GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT
  361 GCTGGGGAGG GAATTAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
  421 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
  481 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
  541 GGGAAACCTG TCGTGCCAGT CGAGAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG
  601 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG
  661 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
  721 GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
  781 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG
  841 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA
  901 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC
  961 GCGAGACGAA AGGGCCTTCG TGATAACGCC TATTTTTTAT AGGGTTAATG TTCAATGAAT
 1021 AATAAATGGG TTTTCCTTTA GAACGTTCAA GGTGCCACTT TTCGGGGGGA AATGGTGCGG
1081 CGGACCCTTA TTTGGTTTAA TTTTTCCTAA AATACCAATT TTCCAAAATA AA
//
Primer: MINT-RAM7_F ()
Sequence file: RDB13474_A5Hee.seq check
>D02692A3_A5He_4_MINT-RAM7_F_D02_11_ABI24.ab1
    1 GATCTACCAT CTCCAGGCCC TTCCCCCAGA CACAAAGGCC TCTGATATGG ACACCAGCTC
   61 CAGTACACTG AGGAAGATCC TCATGGACCC CAAATATGTG TCTGCCACAG GAGTTACGTC
  121 CACAAGTGTC ACTTCAGCCA TTGCAGAGCC CGTGAGTGCG CCTTGTCTGC AGGAGGCGCC
  181 GGCCCCTCCC TGTGACCCTA AGCATCCTCC CTTAGAAGGC GTGTCAGCAG CCGCAGTACC
  241 AAATGCTGAC ACACAAGCCT CAGAGGTTCC GGTGGCTGCC GACAAAGAGA AGGTGGCTCC
  301 GGTCATTGCC CCTAAAATCA CGTCGGTTAT TAGCCGGATG CCCGTTAGCA TTGACCTGGA
  361 GAACTCGCAG AAGATCACGT TGGCAAAGCC AGCTCCTCAG ACCCTGACTG GCCTGGTGAG
  421 TGCCCTGACT GGTCTGGTGA ATGTCTCTCT GGTCCCGGTG AATGCCTTAA AGGGCCCTGT
  481 GAAGGGCTCA GTAGCCACCC TGAAAGGTTT GGTGAGCACC CCTGCTGGGC CCGTGAATCT
  541 CCTAAAGGGG CCCGTGAATG TCCTCACGGG GCCTGTCAAT GTCCTGACTA CTCCTGTGAG
  601 TGCCACAGTG GGCACGGTGA ACGCTGCCCC CGGCCCCGTG ACTGCTGCCT GTGGCGTGAC
  661 GGCCACAACA GGCACAGCAG CCGTGACGGG TGCAGTGACT GCCCCAGCAG CCAAAGGGCA
  721 AGCAGCGGGC CAGTAGCAAT GAAGAACAGC CGCTTTCCAC CCTGGGTCCA TGTCAGTGAT
  781 TGATGACGAC CAGCAGACAC GGACTTCTGG CCGCGGGGCT GCGGTGAATA CATCAGAAGG
  841 GGTCGTGCTG CTGAGCTACT CGGGACAGAA AAACGGGAAG GCTCCAGCGA TCAGTGCTAG
  901 ATCAGCCAGA TCCTCCAGCA GCGCATGGGA CATCGAGTCA AGCAGGTCAG ATCCAGGTTC
  961 CGAGTGAAAG CGCGATCGCA TACACTCTGA ATCGATTCGC ACTAAGGCTG AACTAAGCTT
1021 GCAAGTGGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956. [link to RRC of NBRP]