Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-BOSneo-Myc-MINT deltaN

Expression vector of mouse SPEN/MINT cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources. Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

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提供条件

本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(Kuroda, K., Immunity, 18, 301-312, 2003)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

提供依頼

提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13473_A5Hep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 () Sequence file: RDB13473_A5Hea.seq
>D02692A2_A5He_4_pAxEF_F1_B01_04_ABI24.ab1     1 GGGTGATCGT GAGGAATTCT CTAGCCCTCG ACGGTATCGA TAAGCTTGAT AACACCATGG    61 AGCAAAAGCT CATTTCTGAA GAGGACTTGA GAGAATTCAT GAAGAAGAAG AAAATCAGGA   121 CGGACTCGGA GGGGAAACTG GCTGATAAAA AGGACGAGCG CAGGGAGGAG GAGCAGGAGA   181 GGCAGGAGTT GTTCGCCTCC CGGTTCTTAC ACAGCTCCAT CTTTGAGCAA GACTCCAAGA   241 GGCTGCAGCA CCTTGAGAGG AAGAGCGAGG AGCCCGACCT CCCTCCCGGG AGGCTGTACG   301 GCAGGCAGGC GCCTGAGGGT GCAAACAGCA CGAGTGATTC GGTGCAGGAG CCAGTGGTCC   361 TGTTCCACAG CAGATTCCTG GAGCTGACGC GGATGCAGCA GAAGGAGAAG GAGAAGGACC   421 AGAAGCCCAA AGAGGCTGAG AAGCAGGAAG AGCCGGAGAC TCATCCCAAG ACTCCAGAGC   481 CTGCTGCAGA GACTAAAGAG CCGGAGCCGA AAGCCCCTGT TTCAGCTGGG CTTCCTGCCG   541 TCACCATCAC CGTCGTGACA CCAGAGCCAG CATCCTCCGC CCCTGAGAAG GCTGAGGAGG   601 CAGCGGAGGC TCCTTCACCA GCAGGAGAGA AGCCTGCAGA GCCCGCTCCT GTCTCGGAAG   661 AAACAAAGCT TGTGTCTGAG CCTGCGTCTG TACCCGTGGA GCAGCCCAGG CAGTCAGATG   721 TACCCCCCGG AGAGGACAGC CGGGATAGCC AGGACAGCGC TGCCTTGGCA CCTTCAGCTC   781 CTCAAGAAAG CGCAGCTACT GACGCCGTCC CTTGTGTGAA TGCTGAACCT CTGACTCCCG   841 GAACCACAGT TTCCCAGGTA GAAAGCAGTG TAGACCCCAA ACCTAGCAGT CCCCAGCCCC   901 TTTTCAAAAC TGACTCAGAG GTCCGAGGAA GCCGAAGAGG GGAAAGTGGA GAAGCCAGAC   961 ACAACTCCAA GCACTGAGCC CTGACGCCAC TCAGACGCTG GGGGTTGCAT CAGAGCTCAG  1021 CCTCCTCCGC CTTTCTGAAG GATGTAGAGC CGATTCCTTC CAAGTTCGCT TGCCAAAGGA 1081 CG //
Primer: hGCSF_R () Sequence file: RDB13473_A5Heb.seq
>D02692A2_A5He_4_hGCSF_R_A01_01_ABI24.ab1     1 CGGTAGCATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGGATA TCATCTAGAA    61 CTAGTGGATC CTTTTTTTTT TTTTTTGCAG CAGAGCCTCA GGAAAATGCC CTGAGGTGGC   121 GACCGTTCAG AGCCTCACAC CGAGGCAATG ACAATCATGA GGTGAGGAGA GATGTTGGAG   181 ATGCTGGCAA GGAGGTCAGG GGCCAGCCGG GAGAGGTGAC TCTCGGAGAA CTCACAGGGT   241 GGGAAGATCT GAAGCACGTA GGCAGGCTGA TTGGAGCCAG GGTTGGGGAC ATTGATAATC   301 CCTGCTGCTT GCTTGGCCTG CAGGTAAGTG ATGAAGGCAG CCTTGAGGGA CTCGGTCTGG   361 CTCACCACAT CCTCCTGGTC ACGGCCACAG GGCAGAGCCA GCAGCAGGCA GTAGTCGGTT   421 TCCACTGTCA TTCTCCTGGC GACCCCTTCC AGCTGTGAGG CCTCTAGTCG CATCCTCTGG   481 GCGATCCGCA GTGGGGGACC ACCTTCAGAG AGAGGCAGGG ACCGATGAGC CAGGACATTG   541 TTGCCAGAGA CGAAGTGGAG CTGCACAGCA GCCGTGTCGT TCTTGAGGGC CAGCAGGCCC   601 TGCCACACGA TGGGGTACTT CTTCAGCAGC TGGACCATAT CCACAGGCCG TTGTGGTGTC   661 CCGTGTGGAG AAGACTCTTG TTTGGGAGCT GGCTGGACTT CAGCGTGAGG CAGAGCCAGT   721 CCAGGTGGGG TGCTTGGGCC TGAGTTTGCA GGGACAAACA ATGGCTGCTT TGGGGCAGCC   781 TGAGAGGAAA GAGGGCTTGG GAGGTCTGGC TTCATGGAGA CGGACACAGG GGAGGGGGTG   841 GGCTGGCTGG GCTGTTTCCA CCGGCGCCCT CTGAAGCTGA GCGTGTGGCT CAGATGGCCT   901 GTTTGTGGGA TGGCTGGGAG GCGAGTCTGC TCCACCCCCT CCTGTCTGCG TCCCCTTTGC   961 TTCTTGGGAG ACCTGAGGAA TCTTGGCCAC TCGTGGGCTG GGCAGCTGGC TGAGTGGAGG  1021 GCTGGGCAGG CGGGCCAGTG GTATAGCTGC GTGCCTGCGT AAAGGGGCCA GCCTGTTGCC 1081 AAACTTGTCG C //
Primer: HSV_TK_pA_R () Sequence file: RDB13473_A5Hec.seq
>D02692A2_A5He_4_HSV_TK_pA_R_C01_07_ABI24.ab1     1 CAGACTCGTC AGAAGGCGAT AGAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA    61 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC   121 AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA   181 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA   241 TCCTCGCCGT CGGGCATGCG CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC   301 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT   361 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC   421 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC   481 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA   541 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC   601 TCGTCCTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC   661 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG   721 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT   781 TCAATGGCCG ATCCCATATT GGCTGCAGGG TCGCTCGGTG TTCGAGGCCA CACGCGTCAC   841 CTTAATATGC GAAGTGGACC TGGGACCGCG CCGCCCCGAC TGCATCTGCG TGTTCGAATT   901 CGCCCAATGA CAAGACGCTG GGCGGGGTTT GCTCGACATT GGGTGGAAAC ATTCCAGGCC   961 TGGGTGGAGA GGCCTTTTTT GCTTCCTCTT GCAAAACCCA CACTGCTCGA CATTGGGTGG  1021 AAAACATTCC CAGCCCTGGG TGGGAGAGCT TTTTGCTTCC TCCTTGAAAC ACACTGGCTT 1081 CGAACTGCCG AAGGGGGTAA TGGTTGCCTG GCCAA //
Primer: EF1a-N_R () Sequence file: RDB13473_A5Hed.seq
>D02692A2_A5He_4_EF1a-N_R_E01_13_ABI24.ab1     1 CCCCGGGATG CGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCA ATTCCCACTC    61 CTTTCAAGAC CTAGAAGGTC CATTAGCTGC AAAGATTCCT CTCTGTTTAA AACTTTATCC   121 ATCTTTGCAA AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG   181 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC   241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG   301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA   361 GGGAATTAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC   421 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT   481 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC   541 TGTCGTGCCA GTCGAGAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC   601 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG   661 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG   721 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC   781 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC   841 CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC   901 CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG   961 AAAGGGCCCT CGTGATACGC CTAATTTTTA TAGGTTAATG TCATGAATAA TAATGGGTTT  1021 CTAGACGTCA GGTGGCACTT TTCCGGGGGA ATGTGCGCGA CCCCTATTGT TATTTTTCTA 1081 AATAACCATT CCAAAATAAG G //
Primer: MINT-RAM7_F () Sequence file: RDB13473_A5Hee.seq
>D02692A2_A5He_4_MINT-RAM7_F_D01_10_ABI24.ab1     1 GGAACCCCAA TCTCAGGCCC TTCCCCCAGA CACAAAGGCC TCTGATATGG ACACCAGCTC    61 CAGTACACTG AGGAAGATCC ACATGGACCC CAAATATGTG TCTGCCACAG GAGTTACGTC   121 CACAAGTGTC ACTTCAGCCA TTGCAGAGCC CGTGAGTGCG CCTTGTCTGC AGGAGGCGCC   181 GGCCCCTCCC TGTGACCCTA AGCATCCTCC CTTAGAAGGC GTGTCAGCAG CCGCAGTACC   241 AAATGCTGAC ACACAAGCCT CAGAGGTTCC GGTGGCTGCC GACAAAGAGA AGGTGGCTCC   301 GGTCATTGCC CCTAAAATCA CGTCGGTTAT TAGCCGGATG CCCGTTAGCA TTGACCTGGA   361 GAACTCGCAG AAGATCACGT TGGCAAAGCC AGCTCCTCAG ACCCTGACTG GCCTGGTGAG   421 TGCCCTGACT GGTCTGGTGA ATGTCTCTCT GGTCCCGGTG AATGCCTTAA AGGGCCCTGT   481 GAAGGGCTCA GTAGCCACCC TGAAAGGTTT GGTGAGCACC CCTGCTGGGC CCGTGAATCT   541 CCTAAAGGGG CCCGTGAATG TCCTCACGGG GCCTGTCAAT GTCCTGACTA CTCCTGTGAG   601 TGCCACAGTG GGCACGGTGA ACGCTGCCCC CGGCCCCGTG ACTGCTGCCT GTGGCGTGAC   661 GGCCACAACA GGCACAGCAG CCGTGACGGG TGCAGTGACT GCCCCAGCAG CCAAGGGCAA   721 GCAGCGGGCC AGTAGCAATG AGAACAGCCG CTTCCACCCT GGGTCCATGT CAGTGATTGA   781 TGACCGACCA GCAGACACGG GCTCTGGCGC GGGGCTGCGG GTGAATACAT CAGAAGGGGT   841 CGTGCTGCTG AGCTACTCGG GACAGAAGAC GGAAGGCCCC CAGCGGATCA GTGCTAAGAT   901 CAGCCAGATC CCTCCAGCCA GCGCAATGGA CATCGAGTTT CAGCAGTCAG TATCCAAGTC   961 CCAAGTGAAA GCAGACTCCA TCACCCCGAC CCAGTCTGCA CCCAAAGGCC CTCAGACCCC  1021 CTTCAGCCCT TTGCCAACGT GGACGCCCAT TCTACCTTGG TTACTGGACT GCCCAGAACA 1081 ATACAATGCA TCTCCCGATG AATCGTCTTC GATGAAGAAC CGGAACCGGC CCCAATTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

GNP_filter3_RDBDEP_html_231001.pl