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pEF-BOSneo-Myc-MINT deltaN (#RDB13473)

Expression vector of mouse SPEN/MINT cDNA

Clone info. Expression vector of mouse SPEN/MINT cDNA. deltaN in Figure 1A of Kuroda, K. et al., Immunity 18 (2), 301-312, 2003.
Comment Known difference (at least): Nucleotide sequences of vector-insert junction and variable region were checked and found at least six nucleotide substitutions comparing with NM_019763.2 (cds, 352..11283), G to A at 5074nt (Gly to Arg), T to C at 5095nt (Ser to Pro), A to C at 5161nt (Met to Leu), G to A at 5329nt (Val to Ile), T to C at 4983nt (silent), and C to T at 5067nt (silent).
Vector backbone pEF-BOS Neo (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Mint cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。公表を前提とした学術研究に限る。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(Kuroda, K., Immunity, 18, 301-312, 2003)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13473 pEF-BOSneo-Myc-MINT deltaN DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneo-Myc-MINT deltaN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13473).

Reference section:

Kuroda, K., Han, H., Tani, S., Tanigaki, K., Tun, T., Furukawa, T., Taniguchi, Y., Kurooka, H., Hamada, Y., Toyokuni, S., Honjo, T., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13473_A5Hep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 ()
Sequence file: RDB13473_A5Hea.seq check
>D02692A2_A5He_4_pAxEF_F1_B01_04_ABI24.ab1
    1 GGGTGATCGT GAGGAATTCT CTAGCCCTCG ACGGTATCGA TAAGCTTGAT AACACCATGG
   61 AGCAAAAGCT CATTTCTGAA GAGGACTTGA GAGAATTCAT GAAGAAGAAG AAAATCAGGA
  121 CGGACTCGGA GGGGAAACTG GCTGATAAAA AGGACGAGCG CAGGGAGGAG GAGCAGGAGA
  181 GGCAGGAGTT GTTCGCCTCC CGGTTCTTAC ACAGCTCCAT CTTTGAGCAA GACTCCAAGA
  241 GGCTGCAGCA CCTTGAGAGG AAGAGCGAGG AGCCCGACCT CCCTCCCGGG AGGCTGTACG
  301 GCAGGCAGGC GCCTGAGGGT GCAAACAGCA CGAGTGATTC GGTGCAGGAG CCAGTGGTCC
  361 TGTTCCACAG CAGATTCCTG GAGCTGACGC GGATGCAGCA GAAGGAGAAG GAGAAGGACC
  421 AGAAGCCCAA AGAGGCTGAG AAGCAGGAAG AGCCGGAGAC TCATCCCAAG ACTCCAGAGC
  481 CTGCTGCAGA GACTAAAGAG CCGGAGCCGA AAGCCCCTGT TTCAGCTGGG CTTCCTGCCG
  541 TCACCATCAC CGTCGTGACA CCAGAGCCAG CATCCTCCGC CCCTGAGAAG GCTGAGGAGG
  601 CAGCGGAGGC TCCTTCACCA GCAGGAGAGA AGCCTGCAGA GCCCGCTCCT GTCTCGGAAG
  661 AAACAAAGCT TGTGTCTGAG CCTGCGTCTG TACCCGTGGA GCAGCCCAGG CAGTCAGATG
  721 TACCCCCCGG AGAGGACAGC CGGGATAGCC AGGACAGCGC TGCCTTGGCA CCTTCAGCTC
  781 CTCAAGAAAG CGCAGCTACT GACGCCGTCC CTTGTGTGAA TGCTGAACCT CTGACTCCCG
  841 GAACCACAGT TTCCCAGGTA GAAAGCAGTG TAGACCCCAA ACCTAGCAGT CCCCAGCCCC
  901 TTTTCAAAAC TGACTCAGAG GTCCGAGGAA GCCGAAGAGG GGAAAGTGGA GAAGCCAGAC
  961 ACAACTCCAA GCACTGAGCC CTGACGCCAC TCAGACGCTG GGGGTTGCAT CAGAGCTCAG
 1021 CCTCCTCCGC CTTTCTGAAG GATGTAGAGC CGATTCCTTC CAAGTTCGCT TGCCAAAGGA
1081 CG
//
Primer: hGCSF_R ()
Sequence file: RDB13473_A5Heb.seq check
>D02692A2_A5He_4_hGCSF_R_A01_01_ABI24.ab1
    1 CGGTAGCATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGGATA TCATCTAGAA
   61 CTAGTGGATC CTTTTTTTTT TTTTTTGCAG CAGAGCCTCA GGAAAATGCC CTGAGGTGGC
  121 GACCGTTCAG AGCCTCACAC CGAGGCAATG ACAATCATGA GGTGAGGAGA GATGTTGGAG
  181 ATGCTGGCAA GGAGGTCAGG GGCCAGCCGG GAGAGGTGAC TCTCGGAGAA CTCACAGGGT
  241 GGGAAGATCT GAAGCACGTA GGCAGGCTGA TTGGAGCCAG GGTTGGGGAC ATTGATAATC
  301 CCTGCTGCTT GCTTGGCCTG CAGGTAAGTG ATGAAGGCAG CCTTGAGGGA CTCGGTCTGG
  361 CTCACCACAT CCTCCTGGTC ACGGCCACAG GGCAGAGCCA GCAGCAGGCA GTAGTCGGTT
  421 TCCACTGTCA TTCTCCTGGC GACCCCTTCC AGCTGTGAGG CCTCTAGTCG CATCCTCTGG
  481 GCGATCCGCA GTGGGGGACC ACCTTCAGAG AGAGGCAGGG ACCGATGAGC CAGGACATTG
  541 TTGCCAGAGA CGAAGTGGAG CTGCACAGCA GCCGTGTCGT TCTTGAGGGC CAGCAGGCCC
  601 TGCCACACGA TGGGGTACTT CTTCAGCAGC TGGACCATAT CCACAGGCCG TTGTGGTGTC
  661 CCGTGTGGAG AAGACTCTTG TTTGGGAGCT GGCTGGACTT CAGCGTGAGG CAGAGCCAGT
  721 CCAGGTGGGG TGCTTGGGCC TGAGTTTGCA GGGACAAACA ATGGCTGCTT TGGGGCAGCC
  781 TGAGAGGAAA GAGGGCTTGG GAGGTCTGGC TTCATGGAGA CGGACACAGG GGAGGGGGTG
  841 GGCTGGCTGG GCTGTTTCCA CCGGCGCCCT CTGAAGCTGA GCGTGTGGCT CAGATGGCCT
  901 GTTTGTGGGA TGGCTGGGAG GCGAGTCTGC TCCACCCCCT CCTGTCTGCG TCCCCTTTGC
  961 TTCTTGGGAG ACCTGAGGAA TCTTGGCCAC TCGTGGGCTG GGCAGCTGGC TGAGTGGAGG
 1021 GCTGGGCAGG CGGGCCAGTG GTATAGCTGC GTGCCTGCGT AAAGGGGCCA GCCTGTTGCC
1081 AAACTTGTCG C
//
Primer: HSV_TK_pA_R ()
Sequence file: RDB13473_A5Hec.seq check
>D02692A2_A5He_4_HSV_TK_pA_R_C01_07_ABI24.ab1
    1 CAGACTCGTC AGAAGGCGAT AGAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA
   61 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC
  121 AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA
  181 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA
  241 TCCTCGCCGT CGGGCATGCG CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC
  301 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT
  361 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC
  421 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC
  481 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA
  541 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC
  601 TCGTCCTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC
  661 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG
  721 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT
  781 TCAATGGCCG ATCCCATATT GGCTGCAGGG TCGCTCGGTG TTCGAGGCCA CACGCGTCAC
  841 CTTAATATGC GAAGTGGACC TGGGACCGCG CCGCCCCGAC TGCATCTGCG TGTTCGAATT
  901 CGCCCAATGA CAAGACGCTG GGCGGGGTTT GCTCGACATT GGGTGGAAAC ATTCCAGGCC
  961 TGGGTGGAGA GGCCTTTTTT GCTTCCTCTT GCAAAACCCA CACTGCTCGA CATTGGGTGG
 1021 AAAACATTCC CAGCCCTGGG TGGGAGAGCT TTTTGCTTCC TCCTTGAAAC ACACTGGCTT
1081 CGAACTGCCG AAGGGGGTAA TGGTTGCCTG GCCAA
//
Primer: EF1a-N_R ()
Sequence file: RDB13473_A5Hed.seq check
>D02692A2_A5He_4_EF1a-N_R_E01_13_ABI24.ab1
    1 CCCCGGGATG CGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCA ATTCCCACTC
   61 CTTTCAAGAC CTAGAAGGTC CATTAGCTGC AAAGATTCCT CTCTGTTTAA AACTTTATCC
  121 ATCTTTGCAA AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG
  181 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC
  241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG
  301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA
  361 GGGAATTAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
  421 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
  481 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
  541 TGTCGTGCCA GTCGAGAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
  601 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
  661 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG
  721 CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC
  781 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC
  841 CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC
  901 CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG
  961 AAAGGGCCCT CGTGATACGC CTAATTTTTA TAGGTTAATG TCATGAATAA TAATGGGTTT
 1021 CTAGACGTCA GGTGGCACTT TTCCGGGGGA ATGTGCGCGA CCCCTATTGT TATTTTTCTA
1081 AATAACCATT CCAAAATAAG G
//
Primer: MINT-RAM7_F ()
Sequence file: RDB13473_A5Hee.seq check
>D02692A2_A5He_4_MINT-RAM7_F_D01_10_ABI24.ab1
    1 GGAACCCCAA TCTCAGGCCC TTCCCCCAGA CACAAAGGCC TCTGATATGG ACACCAGCTC
   61 CAGTACACTG AGGAAGATCC ACATGGACCC CAAATATGTG TCTGCCACAG GAGTTACGTC
  121 CACAAGTGTC ACTTCAGCCA TTGCAGAGCC CGTGAGTGCG CCTTGTCTGC AGGAGGCGCC
  181 GGCCCCTCCC TGTGACCCTA AGCATCCTCC CTTAGAAGGC GTGTCAGCAG CCGCAGTACC
  241 AAATGCTGAC ACACAAGCCT CAGAGGTTCC GGTGGCTGCC GACAAAGAGA AGGTGGCTCC
  301 GGTCATTGCC CCTAAAATCA CGTCGGTTAT TAGCCGGATG CCCGTTAGCA TTGACCTGGA
  361 GAACTCGCAG AAGATCACGT TGGCAAAGCC AGCTCCTCAG ACCCTGACTG GCCTGGTGAG
  421 TGCCCTGACT GGTCTGGTGA ATGTCTCTCT GGTCCCGGTG AATGCCTTAA AGGGCCCTGT
  481 GAAGGGCTCA GTAGCCACCC TGAAAGGTTT GGTGAGCACC CCTGCTGGGC CCGTGAATCT
  541 CCTAAAGGGG CCCGTGAATG TCCTCACGGG GCCTGTCAAT GTCCTGACTA CTCCTGTGAG
  601 TGCCACAGTG GGCACGGTGA ACGCTGCCCC CGGCCCCGTG ACTGCTGCCT GTGGCGTGAC
  661 GGCCACAACA GGCACAGCAG CCGTGACGGG TGCAGTGACT GCCCCAGCAG CCAAGGGCAA
  721 GCAGCGGGCC AGTAGCAATG AGAACAGCCG CTTCCACCCT GGGTCCATGT CAGTGATTGA
  781 TGACCGACCA GCAGACACGG GCTCTGGCGC GGGGCTGCGG GTGAATACAT CAGAAGGGGT
  841 CGTGCTGCTG AGCTACTCGG GACAGAAGAC GGAAGGCCCC CAGCGGATCA GTGCTAAGAT
  901 CAGCCAGATC CCTCCAGCCA GCGCAATGGA CATCGAGTTT CAGCAGTCAG TATCCAAGTC
  961 CCAAGTGAAA GCAGACTCCA TCACCCCGAC CCAGTCTGCA CCCAAAGGCC CTCAGACCCC
 1021 CTTCAGCCCT TTGCCAACGT GGACGCCCAT TCTACCTTGG TTACTGGACT GCCCAGAACA
1081 ATACAATGCA TCTCCCGATG AATCGTCTTC GATGAAGAAC CGGAACCGGC CCCAATTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956. [link to RRC of NBRP]