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pEGFP-C1-hLC3B (#RDB13435)

Indicator of autophagy. Expression clone of human LC3B in mammalian cells


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Katagiri, N., Sci. Rep. 5: 8903 (2015). PMID 25754892. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression clone of human LC3B in mammalian cells. Indicator of autophagy by formation of punctate structures of the fluorescent proteins.
Comment Expression was confirmed by the depositor with Western blot and immunofluorescent staining.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Growth conditions 37C, LB + Kan
Gene/insert name Human LC3B cDNA
Depositor|Developer Kimura, Keiji |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep. 5: 8903, 2015)
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必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci Rep. 5: 8903, 2015)。

Catalog # Resource name Shipping form Fee
RDB13435 pEGFP-C1-hLC3B DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-hLC3B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13435).

Reference section:

Katagiri, N., Kuroda, T., Kishimoto, H., Hayashi, Y., Kumazawa, T., Kimura, K., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892. [PubMed] [Article] [RRC of NBRP]