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pEGFP-C1-hLC3B

Indicator of autophagy. Expression clone of human LC3B in mammalian cells

Catalog number RDB13435
Resource name pEGFP-C1-hLC3B
Clone info. Expression clone of human LC3B in mammalian cells. Indicator of autophagy by formation of punctate structures of the fluorescent proteins.
Expression was confirmed by the depositor with Western blot and immunofluorescent staining.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human LC3B cDNA
Depositor Kimura, Keiji |
 
Other clones in our bank human MAP1LC3B (NCBI Gene 81631) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep. 5: 8903, 2015)
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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RDB13435 pEGFP-C1-hLC3B DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Autophagy (English text)

References

original Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13435_A5Ftp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C RDB13435_A5Fta.seq
>13435_13435_A5Ft_EGFP-C_B01_02_ABI08.ab1
    1 CCGGGCCTTC TCGGGCATGG ACGAGCTGTA CAGTCCGGAC TCAGATCTCG AGCTCAAGCT
   61 TCGAATTCGA TGCCGTCGGA GAAGACCTTC AAGCAGCGCC GCACCTTCGA ACAAAGAGTA
  121 GAAGATGTCC GACTTATTCG AGAGCAGCAT CCAACCAAAA TCCCGGTGAT AATAGAACGA
  181 TACAAGGGTG AGAAGCAGCT TCCTGTTCTG GATAAAACAA AGTTCCTTGT ACCTGACCAT
  241 GTCAACATGA GTGAGCTCAT CAAGATAATT AGAAGGCGCT TACAGCTCAA TGCTAATCAG
  301 GCCTTCTTCC TGTTGGTGAA CGGACACAGC ATGGTCAGCG TCTCCACACC AATCTCAGAG
  361 GTGTATGAGA GTGAGAAAGA TGAAGATGGA TTCCTGTACA TGGTCTATGC CTCCCAGGAG
  421 ACGTTCGGGA TGAAATTGTC AGTGTAAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGA
  481 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
  541 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC
  601 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
  661 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA
  721 CGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
  781 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
  841 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
  901 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGGCCCACTA CGTGAAACCA
  961 TCACCCTAAA TCAAGTTTTT TTGGGGGTCG AGGTGCCCGT AAAGCACTAA ATCGGAACCC
 1021 TAAAGGGAGC CCCCCGATTT TAGAGCTTGG ACCGGGAAGC TGCGACCGTG GCGAGAAAGA
1081 ATTAGAAGCG AAGGAGCGGG CGCTAGGGCC GCTTGGGCCA G
//
Primer: CMV-Forward RDB13435_A5Ftb.seq
>13435_13435_A5Ft_CMV-Forward_A01_01_ABI08.ab1
    1 CGTTAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
  841 AGATCTCGAG CTCAAGCTTC GAATTCGATG CCGTCGGAGA AGACCTTCAA GCAGCGCCGC
  901 ACCTTCGAAC AAAGAGTAGA AGATGTCCGA CTTATTCGAG AGCAGCATCC AACCAAAATC
  961 CCGGTTGATA TAGAACGATA CAAAGGGTTG AGAAGCAGCT TTCCCTGTTT CTGGATAAAA
 1021 CAAAGTTTCC TTTGTACCCT GACCCATGTC CAACAATGAG TGAGCTCATC AGATATAGGA
1081 GGCGCTTACA GCCTCAATTG CCTTAATTCC AG
//
Primer: SV40pro_F_V2 RDB13435_A5Ftc.seq
>13435_13435_A5Ft_SV40pro_F_V2_C01_03_ABI08.ab1
    1 CGAGCACCTT TTTTTGGGAG GGCTAGGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA
   61 GGATCCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
  121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
  181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
  241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
  301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
  361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
  421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
  481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
  541 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
  601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
  661 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA
  721 CCGCTATCAG GACATAGCGT TGGGCTACCC GTGATATTGC TGAAGAGCTT TGGGCGGCGA
  781 ATGGGCTGAC CGCTTTCCTC GTGCTTTTAC GGTATCGCCG CTCCCCGATT CGCAGCGCCA
  841 TCGCCTTTCT ATCCGCCTTC TTGACGGAGT TCTTCTGAGC GGGGACTCTG GGGGGTTCGG
  901 AAATGACCGA CCAAGCGACG CCCCAACCCT GCCATTCACG AGATTTCGAT TCCCACCCGC
  961 CCGCCTTCTA TGAAAGGGTT GGGCTTTCGG GAAATCGTTT TTCCGGGGAC GCCGGGCTGG
 1021 GAATGAATCC CTCCTAGCGC GGGGGAATCT CAATGGCTTG GGAAGGTTTC CTTCGGCTCC
 1081 GTACCCCCTT AAGGGGGGGA AGGGCCTAAA CTTGGAAAAG CAACACGTGG AAAAGGGAAG
 1141 AACCAATAAC TCGGAAACGG GTGACCTCCT GCTCGCCTTA ATGTGACACG TGCGCGAATT
1201 AT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892.

2018.10.09

GNP_filter3_RDBDEP_html_180823.pl