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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-hLC3B (#RDB13435)

Indicator of autophagy. Expression clone of human LC3B in mammalian cells

Clone info. Expression clone of human LC3B in mammalian cells. Indicator of autophagy by formation of punctate structures of the fluorescent proteins.
Comment Expression was confirmed by the depositor with Western blot and immunofluorescent staining.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human LC3B cDNA
Depositor|Developer Kimura, Keiji |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep. 5: 8903, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci Rep. 5: 8903, 2015)。

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RDB13435 pEGFP-C1-hLC3B DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-hLC3B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13435).

Reference section:

Katagiri, N., Kuroda, T., Kishimoto, H., Hayashi, Y., Kumazawa, T., Kimura, K., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13435_A5Ftp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert
Sequence file: RDB13435_A5Ftd.seq check
>13435_13435_B3B6F_mVenus-M_F_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNGGCNTCN AGGTGNANTT CAAGANCCGC CNCNACATCG AGGACGGCAG
   61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
  121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
  181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
  241 GCTGTACAAG TCCGGACTCA GATCTCGAGC TCAAGCTTCG AATTCGATGC CGTCGGAGAA
  301 GACCTTCAAG CAGCGCCGCA CCTTCGAACA AAGAGTAGAA GATGTCCGAC TTATTCGAGA
  361 GCAGCATCCA ACCAAAATCC CGGTGATAAT AGAACGATAC AAGGGTGAGA AGCAGCTTCC
  421 TGTTCTGGAT AAAACAAAGT TCCTTGTACC TGACCATGTC AACATGAGTG AGCTCATCAA
  481 GATAATTAGA AGGCGCTTAC AGCTCAATGC TAATCAGGCC TTCTTCCTGT TGGTGAACGG
  541 ACACAGCATG GTCAGCGTCT CCACACCAAT CTCAGAGGTG TATGAGAGTG AGAAAGATGA
  601 AGATGGATTC CTGTACATGG TCTATGCCTC CCAGGAGACG TTCGGGATGA AATTGTCAGT
  661 GTAAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG
  721 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA
  781 CCTGAAACAT AANATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG
  841 TTACNAATAA AGCAATAGCA TCACAAATTT CNCAAATAAA GCATTTTTTT TCNNTGCATT
  901 CTAGTTGTGG TTTGNCCNAA CTCATCAANG NATCTNAANN NNTAAATTGN NNNCGNNNNT
961 ATTTTGNNNA AANTCNNGNN NANNTTTTNN NTNNANNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB13435_A5Fte.seq check
>13435_13435_B3B6F_CMV-Forward_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNTGNNGCN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
   61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
  601 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGANTT
  781 CGTGACCGCC GCCNGGNTCA CTCTCGGCAT GGACGANCTG TACAAGTNCG GANTCAGATC
  841 TCGAGCTCNA GCTTCNAANN CNATGCCGNC NGANAANNNN NNCNNGCAGC NCCNNACCNN
  901 CNNNNNAAGA GTANANNANG NCCNNNTTAN NCNANNNCNN CNTNNNNNCN NAANNCCNNN
961 NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB13435_A5Ftf.seq check
>13435_13435_B3B6F_SV40pro_ori_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGC CTCGGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT
  121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
  181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
  241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
  301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
  361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
  421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
  481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
  541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
  601 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG
  661 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG
  721 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCANGACAT
  781 AGCGTTGGCT ACCCGTGNTA TTGCTGAAGA GCTTGGCGGC GAATGGGNCT GACCGCTTCC
  841 TCGTGCTTTA CGGTATCGCC GCTCCCNATT CNCAGCGCAT CGCCNTCNAT CGCCTTCTNG
  901 ACNAGTTCTT CTGANCGGGN NTCNGGGGGT TCGAAATGAN CNACCNANCN NCNCCNNNNN
  961 NGNCNNTCNC NANATTTNNA NNNNNNCNNN CNNNCNTNNN NNNAANNGNN NNGNNNTNNN
1021 NNNNNNTTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892. [link to RRC of NBRP]