Sequencing and PCR primers for primer information.
Indicator of autophagy. Expression clone of human LC3B in mammalian cells
| Catalog number | RDB13435 |
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| Resource name | pEGFP-C1-hLC3B |
| Clone info. | Expression clone of human LC3B in mammalian cells. Indicator of autophagy by formation of punctate structures of the fluorescent proteins. Expression was confirmed by the depositor with Western blot and immunofluorescent staining. |
| Vector backbone | pEGFP-C1 (Plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells). |
| Gene/insert name | Human LC3B cDNA |
| Depositor | Kimura, Keiji | |
| Other clones in our bank | human MAP1LC3B (NCBI Gene 81631) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep. 5: 8903, 2015) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13435 | pEGFP-C1-hLC3B | DNA solution |
Electronic file
Featured content
| Featured content | Autophagy (English text) |
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References
| original | Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13435_A5Ftp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: EGFP-C | RDB13435_A5Fta.seq |
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>13435_13435_A5Ft_EGFP-C_B01_02_ABI08.ab1 1 CCGGGCCTTC TCGGGCATGG ACGAGCTGTA CAGTCCGGAC TCAGATCTCG AGCTCAAGCT 61 TCGAATTCGA TGCCGTCGGA GAAGACCTTC AAGCAGCGCC GCACCTTCGA ACAAAGAGTA 121 GAAGATGTCC GACTTATTCG AGAGCAGCAT CCAACCAAAA TCCCGGTGAT AATAGAACGA 181 TACAAGGGTG AGAAGCAGCT TCCTGTTCTG GATAAAACAA AGTTCCTTGT ACCTGACCAT 241 GTCAACATGA GTGAGCTCAT CAAGATAATT AGAAGGCGCT TACAGCTCAA TGCTAATCAG 301 GCCTTCTTCC TGTTGGTGAA CGGACACAGC ATGGTCAGCG TCTCCACACC AATCTCAGAG 361 GTGTATGAGA GTGAGAAAGA TGAAGATGGA TTCCTGTACA TGGTCTATGC CTCCCAGGAG 421 ACGTTCGGGA TGAAATTGTC AGTGTAAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGA 481 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA 541 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC 601 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 661 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA 721 CGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG 781 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC 841 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA 901 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGGCCCACTA CGTGAAACCA 961 TCACCCTAAA TCAAGTTTTT TTGGGGGTCG AGGTGCCCGT AAAGCACTAA ATCGGAACCC 1021 TAAAGGGAGC CCCCCGATTT TAGAGCTTGG ACCGGGAAGC TGCGACCGTG GCGAGAAAGA 1081 ATTAGAAGCG AAGGAGCGGG CGCTAGGGCC GCTTGGGCCA G // |
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| Primer: CMV-Forward | RDB13435_A5Ftb.seq |
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>13435_13435_A5Ft_CMV-Forward_A01_01_ABI08.ab1 1 CGTTAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG 121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC 181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG 241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG 301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC 361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG 421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC 481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG 541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC 601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC 661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG 721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG 781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC 841 AGATCTCGAG CTCAAGCTTC GAATTCGATG CCGTCGGAGA AGACCTTCAA GCAGCGCCGC 901 ACCTTCGAAC AAAGAGTAGA AGATGTCCGA CTTATTCGAG AGCAGCATCC AACCAAAATC 961 CCGGTTGATA TAGAACGATA CAAAGGGTTG AGAAGCAGCT TTCCCTGTTT CTGGATAAAA 1021 CAAAGTTTCC TTTGTACCCT GACCCATGTC CAACAATGAG TGAGCTCATC AGATATAGGA 1081 GGCGCTTACA GCCTCAATTG CCTTAATTCC AG // |
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| Primer: SV40pro_F_V2 | RDB13435_A5Ftc.seq |
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>13435_13435_A5Ft_SV40pro_F_V2_C01_03_ABI08.ab1 1 CGAGCACCTT TTTTTGGGAG GGCTAGGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA 61 GGATCCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 541 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 661 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA 721 CCGCTATCAG GACATAGCGT TGGGCTACCC GTGATATTGC TGAAGAGCTT TGGGCGGCGA 781 ATGGGCTGAC CGCTTTCCTC GTGCTTTTAC GGTATCGCCG CTCCCCGATT CGCAGCGCCA 841 TCGCCTTTCT ATCCGCCTTC TTGACGGAGT TCTTCTGAGC GGGGACTCTG GGGGGTTCGG 901 AAATGACCGA CCAAGCGACG CCCCAACCCT GCCATTCACG AGATTTCGAT TCCCACCCGC 961 CCGCCTTCTA TGAAAGGGTT GGGCTTTCGG GAAATCGTTT TTCCGGGGAC GCCGGGCTGG 1021 GAATGAATCC CTCCTAGCGC GGGGGAATCT CAATGGCTTG GGAAGGTTTC CTTCGGCTCC 1081 GTACCCCCTT AAGGGGGGGA AGGGCCTAAA CTTGGAAAAG CAACACGTGG AAAAGGGAAG 1141 AACCAATAAC TCGGAAACGG GTGACCTCCT GCTCGCCTTA ATGTGACACG TGCGCGAATT 1201 AT // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892. |
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2018.10.09
GNP_filter3_RDBDEP_html_180823.pl