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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-hLC3B

Indicator of autophagy. Expression clone of human LC3B in mammalian cells

Catalog number RDB13435
Resource name pEGFP-C1-hLC3B
Clone info. Expression clone of human LC3B in mammalian cells. Indicator of autophagy by formation of punctate structures of the fluorescent proteins.
Comment Expression was confirmed by the depositor with Western blot and immunofluorescent staining.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human LC3B cDNA
Depositor|Developer Kimura, Keiji |
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External Database
Human LC3B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep. 5: 8903, 2015)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci Rep. 5: 8903, 2015)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13435 pEGFP-C1-hLC3B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Autophagy (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13435_A5Ftp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C
          Sequence file: RDB13435_A5Fta.seq check
          >13435_13435_A5Ft_EGFP-C_B01_02_ABI08.ab1
              1 CCGGGCCTTC TCGGGCATGG ACGAGCTGTA CAGTCCGGAC TCAGATCTCG AGCTCAAGCT
             61 TCGAATTCGA TGCCGTCGGA GAAGACCTTC AAGCAGCGCC GCACCTTCGA ACAAAGAGTA
            121 GAAGATGTCC GACTTATTCG AGAGCAGCAT CCAACCAAAA TCCCGGTGAT AATAGAACGA
            181 TACAAGGGTG AGAAGCAGCT TCCTGTTCTG GATAAAACAA AGTTCCTTGT ACCTGACCAT
            241 GTCAACATGA GTGAGCTCAT CAAGATAATT AGAAGGCGCT TACAGCTCAA TGCTAATCAG
            301 GCCTTCTTCC TGTTGGTGAA CGGACACAGC ATGGTCAGCG TCTCCACACC AATCTCAGAG
            361 GTGTATGAGA GTGAGAAAGA TGAAGATGGA TTCCTGTACA TGGTCTATGC CTCCCAGGAG
            421 ACGTTCGGGA TGAAATTGTC AGTGTAAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGA
            481 TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
            541 AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC
            601 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
            661 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA
            721 CGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
            781 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
            841 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
            901 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGGCCCACTA CGTGAAACCA
            961 TCACCCTAAA TCAAGTTTTT TTGGGGGTCG AGGTGCCCGT AAAGCACTAA ATCGGAACCC
           1021 TAAAGGGAGC CCCCCGATTT TAGAGCTTGG ACCGGGAAGC TGCGACCGTG GCGAGAAAGA
          1081 ATTAGAAGCG AAGGAGCGGG CGCTAGGGCC GCTTGGGCCA G
          //
          Primer: CMV-Forward
          Sequence file: RDB13435_A5Ftb.seq check
          >13435_13435_A5Ft_CMV-Forward_A01_01_ABI08.ab1
              1 CGTTAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
            121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
            181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
            241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
            301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
            361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
            421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
            481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
            541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
            601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
            661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
            721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
            781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
            841 AGATCTCGAG CTCAAGCTTC GAATTCGATG CCGTCGGAGA AGACCTTCAA GCAGCGCCGC
            901 ACCTTCGAAC AAAGAGTAGA AGATGTCCGA CTTATTCGAG AGCAGCATCC AACCAAAATC
            961 CCGGTTGATA TAGAACGATA CAAAGGGTTG AGAAGCAGCT TTCCCTGTTT CTGGATAAAA
           1021 CAAAGTTTCC TTTGTACCCT GACCCATGTC CAACAATGAG TGAGCTCATC AGATATAGGA
          1081 GGCGCTTACA GCCTCAATTG CCTTAATTCC AG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13435_A5Ftc.seq check
          >13435_13435_A5Ft_SV40pro_F_V2_C01_03_ABI08.ab1
              1 CGAGCACCTT TTTTTGGGAG GGCTAGGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA
             61 GGATCCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
            121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
            181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
            241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
            301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
            361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
            421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
            481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
            541 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
            601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
            661 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA
            721 CCGCTATCAG GACATAGCGT TGGGCTACCC GTGATATTGC TGAAGAGCTT TGGGCGGCGA
            781 ATGGGCTGAC CGCTTTCCTC GTGCTTTTAC GGTATCGCCG CTCCCCGATT CGCAGCGCCA
            841 TCGCCTTTCT ATCCGCCTTC TTGACGGAGT TCTTCTGAGC GGGGACTCTG GGGGGTTCGG
            901 AAATGACCGA CCAAGCGACG CCCCAACCCT GCCATTCACG AGATTTCGAT TCCCACCCGC
            961 CCGCCTTCTA TGAAAGGGTT GGGCTTTCGG GAAATCGTTT TTCCGGGGAC GCCGGGCTGG
           1021 GAATGAATCC CTCCTAGCGC GGGGGAATCT CAATGGCTTG GGAAGGTTTC CTTCGGCTCC
           1081 GTACCCCCTT AAGGGGGGGA AGGGCCTAAA CTTGGAAAAG CAACACGTGG AAAAGGGAAG
           1141 AACCAATAAC TCGGAAACGG GTGACCTCCT GCTCGCCTTA ATGTGACACG TGCGCGAATT
          1201 AT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Katagiri, N., The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci. Rep. 5: 8903 (2015). PMID 25754892.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl