Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-hLC3B

Indicator of autophagy. Expression clone of human LC3B in mammalian cells

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci Rep. 5: 8903, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sci Rep. 5: 8903, 2015)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13435_A5Ftp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574) Region: EGFP 3',insert Sequence file: RDB13435_A5Ftd.seq
>13435_13435_B3B6F_mVenus-M_F_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNGGCNTCN AGGTGNANTT CAAGANCCGC CNCNACATCG AGGACGGCAG    61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT   121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG   181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA   241 GCTGTACAAG TCCGGACTCA GATCTCGAGC TCAAGCTTCG AATTCGATGC CGTCGGAGAA   301 GACCTTCAAG CAGCGCCGCA CCTTCGAACA AAGAGTAGAA GATGTCCGAC TTATTCGAGA   361 GCAGCATCCA ACCAAAATCC CGGTGATAAT AGAACGATAC AAGGGTGAGA AGCAGCTTCC   421 TGTTCTGGAT AAAACAAAGT TCCTTGTACC TGACCATGTC AACATGAGTG AGCTCATCAA   481 GATAATTAGA AGGCGCTTAC AGCTCAATGC TAATCAGGCC TTCTTCCTGT TGGTGAACGG   541 ACACAGCATG GTCAGCGTCT CCACACCAAT CTCAGAGGTG TATGAGAGTG AGAAAGATGA   601 AGATGGATTC CTGTACATGG TCTATGCCTC CCAGGAGACG TTCGGGATGA AATTGTCAGT   661 GTAAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA TCATAATCAG   721 CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA   781 CCTGAAACAT AANATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG   841 TTACNAATAA AGCAATAGCA TCACAAATTT CNCAAATAAA GCATTTTTTT TCNNTGCATT   901 CTAGTTGTGG TTTGNCCNAA CTCATCAANG NATCTNAANN NNTAAATTGN NNNCGNNNNT 961 ATTTTGNNNA AANTCNNGNN NANNTTTTNN NTNNANNNNN NNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB13435_A5Fte.seq
>13435_13435_B3B6F_CMV-Forward_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNTGNNGCN NTGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA    61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA TGGTGAGCAA   121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA   181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC   241 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC   301 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT   361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA   421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT   481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA   541 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT   601 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA   661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC   721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGANTT   781 CGTGACCGCC GCCNGGNTCA CTCTCGGCAT GGACGANCTG TACAAGTNCG GANTCAGATC   841 TCGAGCTCNA GCTTCNAANN CNATGCCGNC NGANAANNNN NNCNNGCAGC NCCNNACCNN   901 CNNNNNAAGA GTANANNANG NCCNNNTTAN NCNANNNCNN CNTNNNNNCN NAANNCCNNN 961 NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13435_A5Ftf.seq
>13435_13435_B3B6F_SV40pro_ori_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC CTCGGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT   121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG   181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC   241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA   301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT   361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA   421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG   481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT   541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA   601 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG   661 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG   721 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCANGACAT   781 AGCGTTGGCT ACCCGTGNTA TTGCTGAAGA GCTTGGCGGC GAATGGGNCT GACCGCTTCC   841 TCGTGCTTTA CGGTATCGCC GCTCCCNATT CNCAGCGCAT CGCCNTCNAT CGCCTTCTNG   901 ACNAGTTCTT CTGANCGGGN NTCNGGGGGT TCGAAATGAN CNACCNANCN NCNCCNNNNN   961 NGNCNNTCNC NANATTTNNA NNNNNNCNNN CNNNCNTNNN NNNAANNGNN NNGNNNTNNN 1021 NNNNNNTTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl