Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-DEST51_mm S177A Pdlim5 (#RDB13419)

Expression vector of mouse Pdlim5 (S177A) in mammalian cell

Clone info. Expression vector of mouse Pdlim5 (S177A) in mammalian cell.
Comment Expression was confirmed by the depositor with immunoblotting. Gateway(R) mammalian expression clone.
Vector backbone pEF-DEST51 (Plasmid)
Size of vector backbone 7.5 kb
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cells)
Gene/insert name Mouse Pdlim5 cDNA
Depositor|Developer Tsukamoto, Osamu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat Commun. 6: 6137, 2015). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Remarks An additional fee for license fee of Gateway(R) Expression Vector is required.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nat Commun. 6: 6137, 2015)。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。
備考 Gateway(R)発現ベクター。ライセンス料相当額を負担して頂きます。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13419 pEF-DEST51_mm S177A Pdlim5 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF-DEST51_mm S177A Pdlim5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13419).

Reference section:

Yan, Y., Tsukamoto, O., Nakano, A., Kato, H., Kioka, H., Ito, N., Higo, S., Yamazaki, S., Shintani, Y., Matsuoka, K., Liao, Y., Asanuma, H., Asakura, M., Takafuji, K., Minamino, T., Asano, Y., Kitakaze, M., Takashima, S.., Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5. Nat. Commun. 6: 6137 (2015). PMID 25635515. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Yan, Y., Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5. Nat. Commun. 6: 6137 (2015). PMID 25635515. [link to RRC of NBRP]