Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N10Q, N236Q subustitution.
Clone info. | Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N10Q, N236Q subustitution. |
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Comment | RTAdelata: ricin nontoxic allele that lacks the critical residues for its toxicity of ricin A chain. |
Vector backbone | pEF/myc/ER (Plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | Ricinus communis ricin A chain |
Depositor|Developer | suzuki, tadashi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015)) |
Remarks | ((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 寄託者による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は提供承諾書を用いて、事前に寄託者の承認を得る。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))。 |
備考 | 【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB13415 | pEF/RTAdeltaN10QN236Q/V5-myc/ER | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pEF/RTAdeltaN10QN236Q/V5-myc/ER was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13415). |
Reference section:
Huang, C., Harada, Y., Hosomi, A., Masahara-Negishi, Y., Seino, J., Fujihira, H., Funakoshi, Y., Suzuki, T., Dohmae, N., Suzuki, T., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP] |
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