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pEF/RTAdeltaN236Q/V5-myc/ER (#RDB13414)

Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N236Q substitution.

Clone info. Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N236Q substitution.
Comment RTAdelata: ricin nontoxic allele that lacks the critical residues for its toxicity of ricin A chain.
Vector backbone pEF/myc/ER (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Ricinus communis ricin A chain
Depositor|Developer suzuki, tadashi |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13414 pEF/RTAdeltaN236Q/V5-myc/ER DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF/RTAdeltaN236Q/V5-myc/ER was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13414).

Reference section:

Huang, C., Harada, Y., Hosomi, A., Masahara-Negishi, Y., Seino, J., Fujihira, H., Funakoshi, Y., Suzuki, T., Dohmae, N., Suzuki, T., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13414_A5E1p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB13414_A5E1a.seq check
>13414-13414_2_EF1a-C_F_B02_05.ab1
    1 TCAGGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGTG GCCACCATGG GATGGAGCTG
  121 TATCATCCTC TTCTTGGTAG CAACAGCTAC AGGTAAGGGG TTAACAGTAG CAGGCTTGAG
  181 GTCTGGACAT ATATATGGGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGCGCGC
  241 ACTCCCAGGT CCAACTGCAG ATATTCCCCA AACAATACCC AATTATAAAC TTTACCACAG
  301 CGGGTGCCAC TGTGCAAAGC TACACAAACT TTATCAGAGC TGTTCGCGGT CGTTTAACAA
  361 CTGGAGCTGA TGTGAGACAT GAAATACCAG TGTTGCCAAA CAGAGTTGGT TTGCCTATAA
  421 ACCAACGGTT TATTTTAGTT GAACTCTCAA ATCATGCAGA GCTTTCTGTT ACATTAGCCC
  481 TGGATGTCAC CAATGCATAT GTGGTCGGCT ACCGTGCTGG AAATAGCGCA TATTTCTTTC
  541 ATCCTGACAA TCAGGAAGAT GCAGAAGCAA TCACTCATCT TTTCACTGAT GTTCAAAATC
  601 GATATACATT CGCCTTTGGT GGTAATTATG ATAGACTTGA ACAACTTGCT GGTAATCTGA
  661 GAGAAAATAT CGAGTTGGGA AATGGTCCAC TAGAGGAGGC TATCTCAGCG CTTTATTATT
  721 ACAGTACTGG TGGCACTCAG CTTCCAACTC TGGCTCGTTC CTTTATAATT TGCATCCAAA
  781 TGATTTTCCA ATATATTGAG GGAGAAATGC GCACGAGAAT TAGGTACAAC CGGAGATCTG
  841 CACCAGATCC TAGCGTAATT ACACTTGAGA ATAGTTGGGG GAGACTTTCC ACTGCAATTC
  901 AAGAGTCTAA CCAAGGAGCC TTTGCTAGTC CAATTCAACT GCAAAGACGT CAAGGTTCCA
  961 AATTCAGTGT GTACGATGTG AGTATATTAA TCCCTATCAT AGCTCTCATG GGTGTATAGA
 1021 TGCGCACCTC CACCCATCGT CACAGTTTTC TCGAGATCAA ACGGGGGTAA GCCTATCCCT
1081 ATCTCTCATC CGATCTCGAT TCTACCGGAC CGCAGAAAGC AAAAAAAACG TA
//
Primer: BGH_rev
Sequence file: RDB13414_A5E1b.seq check
>13414-13414_2_BGH-rev_C02_08.ab1
    1 GTTACAGAAT CAGCTCGTGC TTCTCGCTTG CGGCCCCATT CAGATCCTCT TCTGAGATGA
   61 GTTTTTGTTC TGCGGCCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCCC
  121 GTTTGATCTC GAGAAACTGT GACGATGGTG GAGGTGCGCA TCTATACACC ATGAGAGCTA
  181 TGATAGGGAT TAATATACTC ACATCGTACA CACTGAATTT GGAACCTTGA CGTCTTTGCA
  241 GTTGAATTGG ACTAGCAAAG GCTCCTTGGT TAGACTCTTG AATTGCAGTG GAAAGTCTCC
  301 CCCAACTATT CTCAAGTGTA ATTACGCTAG GATCTGGTGC AGATCTCCGG TTGTACCTAA
  361 TTCTCGTGCG CATTTCTCCC TCAATATATT GGAAAATCAT TTGGATGCAA ATTATAAAGG
  421 AACGAGCCAG AGTTGGAAGC TGAGTGCCAC CAGTACTGTA ATAATAAAGC GCTGAGATAG
  481 CCTCCTCTAG TGGACCATTT CCCAACTCGA TATTTTCTCT CAGATTACCA GCAAGTTGTT
  541 CAAGTCTATC ATAATTACCA CCAAAGGCGA ATGTATATCG ATTTTGAACA TCAGTGAAAA
  601 GATGAGTGAT TGCTTCTGCA TCTTCCTGAT TGTCAGGATG AAAGAAATAT GCGCTATTTC
  661 CAGCACGGTA GCCGACCACA TATGCATTGG TGACATCCAG GGCTAATGTA ACAGAAAGCT
  721 CTGCATGATT TGAGAGTTCA ACTAAAATAA ACCGTTGGTT TATAGGCAAA CCAACTCTGT
  781 TTGGCAACAC TGGTATTTCA TGTCTCACAT CAGCTCCAGT TGTTAAACGA CCGCGAACAG
  841 CTCTGATAAA GTTTGTGTAG CTTTGCACAG TGGCACCCGC TGTGGTAAAG TTTATAATTG
  901 GGTATTGTTT GGGGGAATAT CTGCAGTTGG ACCTGGGAGT GCGCGCCTGT GGAGAGAAAG
  961 GCAAAGTGGA TGTCATTGTC ACCCATATAT ATGTCCAGAC CCTCAAGCCC TGCTACTGTT
 1021 TAACCCCCTT ACCCTGTAGC TGTTTGCTAC CCAAAGAAAG AGGATGGATA CAGGCTCCAA
 1081 TCCCCATGGA TGCAACGTGT CAACGACACC TGAAAATGGT AGGAAAAAAA AACCTTTTGG
1141 CTAC
//
Primer: SV40pro_F
Sequence file: RDB13414_A5E1c.seq check
>13414-13414_2_SV40pro_F_D02_11.ab1
    1 ACTGTCGGTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCCCC CCGGGAGGTC CACAATGGTT GAACAAGATG GATTGCACGC AGGTTCTCCG
  121 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT
  181 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC
  241 CTGTCCGGTG CCCTGAATGA ACTCCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
  301 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG
  361 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA
  421 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA
  481 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT
  541 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
  601 AGGCTCAAGG CGCGTATGCC CGACGGCGAG GATCTCGTCG TGACTCATGG CGATGCCTGC
  661 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG
  721 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT
  781 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG
  841 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA
  901 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCCTT
  961 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGGCTGGGAT GATCCTCCAG
 1021 CGCGGGGATC TCATGCTTGG AGTCCTCCGC CACCCCAACT GGTTATGGCA GCCTTATAAT
 1081 TGATACGATA AAGCCAATAG CCATTCACGA AGTTCACTAA TAAAGGCATT CACGTGCAAT
1141 TCCGTTAGCG ATG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP]