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pEF/RTAdeltaN10Q/V5-myc/ER

Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N10Q substitution.

Catalog number RDB13413
Resource name pEF/RTAdeltaN10Q/V5-myc/ER
Clone info. Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N10Q substitution.
Comment RTAdelata: ricin nontoxic allele that lacks the critical residues for its toxicity of ricin A chain.
Vector backbone pEF/myc/ER (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Ricinus communis ricin A chain
Depositor|Developer Suzuki, Tadashi |
Other clones in our bank

External Database
Ricinus communis ricin A chain

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form by depositor [Word]
            Remarks ((Additional form)) Approval Form(FormD)
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は提供承諾書を用いて、事前に寄託者の承認を得る。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))。
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            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            寄託者による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD)
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB13413 pEF/RTAdeltaN10Q/V5-myc/ER Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922.

            2022.05.17

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