Resource data sheet
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pEF/RTAdelta/myc/ER (#RDB13411)

Expression clone of Ricin A chain nontoxic mutant (RTAdelta).

Clone info. Expression clone of Ricin A chain nontoxic mutant (RTAdelta).
Comment RTAdelata: ricin nontoxic allele that lacks the critical residues for its toxicity of ricin A chain.
Vector backbone pEF/myc/ER (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Ricinus communis ricin A chain
Depositor|Developer suzuki, tadashi |
 

Distribution information

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Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は提供承諾書を用いて、事前に寄託者の承認を得る。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13411 pEF/RTAdelta/myc/ER Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF/RTAdelta/myc/ER was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13411).

Reference section:

Huang, C., Harada, Y., Hosomi, A., Masahara-Negishi, Y., Seino, J., Fujihira, H., Funakoshi, Y., Suzuki, T., Dohmae, N., Suzuki, T., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922. [link to RRC of NBRP]