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pGEX6p-1-SOD1_G93A

E coli expression vector for GST-fused human G93A SOD1. (IPTG induction)

Catalog number RDB13394
Resource name pGEX6p-1-SOD1_G93A
Clone info. E coli expression vector for GST-fused human SOD1 (IPTG induction). G93A mutation correlating familial ALS.
Expression was confirmed by the depositor with SDS-PAGE confirming products size (43 kDa) and Western blotting using anti-SOD1 antibody. BL21pLys is recommended for protein production.
Vector backbone pGEX6p-1 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor Urushitani, Makoto |
 
Other clones in our bank human SOD1 (NCBI Gene 6647) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13394 pGEX6p-1-SOD1_G93A DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

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References

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13394_A5Es.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F RDB13394_A5Esa.seq
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Please visit Sequencing and PCR primers for primer information.


References

References

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

2018.12.10

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