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pGEX6p-1-SOD1_G93A

E coli expression vector for GST-fused human G93A SOD1. (IPTG induction)

Catalog number RDB13394
Resource name pGEX6p-1-SOD1_G93A
Clone info. E coli expression vector for GST-fused human SOD1 (IPTG induction). G93A mutation correlating familial ALS.
Comment Expression was confirmed by the depositor with SDS-PAGE confirming products size (43 kDa) and Western blotting using anti-SOD1 antibody. BL21pLys is recommended for protein production.
Vector backbone pGEX6p-1 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
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External Database
human Superoxide dismutase 1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB13394 pGEX6p-1-SOD1_G93A DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13394_A5Es.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F
          Sequence file: RDB13394_A5Esa.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          2022.05.17

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