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pEGFP-SOD1_G93A (#RDB13392)

Mammalian expression vector for human G93A SOD1 with EGFP tag at C-terminus.

Clone info. Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G93A mutation correlating familial ALS.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. Known differences (at least): C to T substitution at 187nt (Gly, synonym) of NM_000454.4.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13392 pEGFP-SOD1_G93A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-SOD1_G93A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13392).

Reference section:

Urushitani, M., Ezzi, S.A., Matsuo, A., Tooyama, I., Julien, J.P., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13392_A5Edp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Sequence file: RDB13392_A5Eda.seq check
>13392_13392_A9G8F_2_CMV-Forward_E03_15_ABI24.ab1
    1 TCCGCCCCCA TTTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
   61 AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT CGAGCTCAAG
  121 CTTCGAATTC GCCATGGCGA CGAAGGCCGT GTGCGTGCTG AAGGGCGACG GTCCAGTGCA
  181 GGGCATCATC AATTTCGAGC AGAAGGAAAG TAATGGACCA GTGAAGGTGT GGGGAAGCAT
  241 TAAAGGACTG ACTGAAGGCC TGCATGGATT CCATGTTCAT GAGTTTGGAG ATAATACAGC
  301 AGGCTGTACC AGTGCAGGTC CTCACTTTAA TCCTCTATCC AGAAAACACG GTGGGCCAAA
  361 GGATGAAGAG AGGCATGTTG GAGACTTGGG CAATGTGACT GCTGACAAAG ATGCTGTGGC
  421 CGATGTGTCT ATTGAAGATT CTGTGATCTC ACTCTCAGGA GACCATTGCA TCATTGGCCG
  481 CACACTGGTG GTCCATGAAA AAGCAGATGA CTTGGGCAAA GGTGGAAATG AAGAAAGTAC
  541 AAAGACAGGA AACGCTGGAA GTCGTTTGGC TTGTGGTGTA ATTGGGATCG CCCAAGGATC
  601 CATCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
  661 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA
  721 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
  781 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA
  841 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
  901 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
  961 CGACACCCTG GTGAACCGCA TCGAGCTGAA AGGCATCGAC TTCAAGAGGG ACGGCAACAT
 1021 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCAACAA CGGTCTATTA TCATTGGCCG
 1081 ACAAGCAGAA GAAACCGCAT CCAAGTGACC TTCAAGGATC GGCACAACAT CGAAGGGAAC
1141 GGGCCAGACG CGGGG
//
Primer: SV40pro_F_V2
Sequence file: RDB13392_A5Edb.seq check
>13392_13392_A9G8F_2_SV40pro_F_V2_G08_07_ABI08.ab1
    1 TAGGAGCTTT TTTTGGGAAG GGCTTAGGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG
   61 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
  121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
  181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
  241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
  301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
  361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
  421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
  481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
  541 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
  601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
  661 CATGGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGGCG
  721 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT TGCCTGAAAG AGCTTGGCGG
  781 CGAATGGGGC TGGACCCGCT TCCCTCGTGC TTTTACCGTT ATCGCCGCTC CCCGATTTCC
  841 GCAACGCCAT CGCCCTTCTA TCCGCCTTCT TGACAAAGTT TCTTCTGAAG CCGGGGACTT
901 TGGGGGGTTT CGAAAATGAA CCGAACCAAA C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP]