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pEGFP-SOD1_G93A (#RDB13392)

Mammalian expression vector for human G93A SOD1 with EGFP tag at C-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Urushitani, M., FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G93A mutation correlating familial ALS.
Comment Expression was confirmed by the depositor with Western blotting of transfected mammalian cells using commercially available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. Known differences (at least): C to T substitution at 187nt (Gly, synonym) of NM_000454.4.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Growth conditions 37C, LB + Kan
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。

Catalog # Resource name Shipping form Fee
RDB13392 pEGFP-SOD1_G93A DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-SOD1_G93A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13392).

Reference section:

Urushitani, M., Ezzi, S.A., Matsuo, A., Tooyama, I., Julien, J.P., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [PubMed] [Article] [RRC of NBRP]