Mammalian expression vector for human G93A SOD1 with EGFP tag at C-terminus.
Clone info. | Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G93A mutation correlating familial ALS. |
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Comment | Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. Known differences (at least): C to T substitution at 187nt (Gly, synonym) of NM_000454.4. |
Vector backbone | pEGFP-N3 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human Superoxide dismutase 1 cDNA |
Depositor|Developer | Urushitani, Makoto | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008)) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13392 | pEGFP-SOD1_G93A | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEGFP-SOD1_G93A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13392). |
Reference section:
Urushitani, M., Ezzi, S.A., Matsuo, A., Tooyama, I., Julien, J.P., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13392_A5Edp1-2.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward Sequence file: RDB13392_A5Eda.seq ![]() |
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>13392_13392_A9G8F_2_CMV-Forward_E03_15_ABI24.ab1 1 TCCGCCCCCA TTTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG 61 AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT CGAGCTCAAG 121 CTTCGAATTC GCCATGGCGA CGAAGGCCGT GTGCGTGCTG AAGGGCGACG GTCCAGTGCA 181 GGGCATCATC AATTTCGAGC AGAAGGAAAG TAATGGACCA GTGAAGGTGT GGGGAAGCAT 241 TAAAGGACTG ACTGAAGGCC TGCATGGATT CCATGTTCAT GAGTTTGGAG ATAATACAGC 301 AGGCTGTACC AGTGCAGGTC CTCACTTTAA TCCTCTATCC AGAAAACACG GTGGGCCAAA 361 GGATGAAGAG AGGCATGTTG GAGACTTGGG CAATGTGACT GCTGACAAAG ATGCTGTGGC 421 CGATGTGTCT ATTGAAGATT CTGTGATCTC ACTCTCAGGA GACCATTGCA TCATTGGCCG 481 CACACTGGTG GTCCATGAAA AAGCAGATGA CTTGGGCAAA GGTGGAAATG AAGAAAGTAC 541 AAAGACAGGA AACGCTGGAA GTCGTTTGGC TTGTGGTGTA ATTGGGATCG CCCAAGGATC 601 CATCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 661 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA 721 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 781 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA 841 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG 901 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 961 CGACACCCTG GTGAACCGCA TCGAGCTGAA AGGCATCGAC TTCAAGAGGG ACGGCAACAT 1021 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCAACAA CGGTCTATTA TCATTGGCCG 1081 ACAAGCAGAA GAAACCGCAT CCAAGTGACC TTCAAGGATC GGCACAACAT CGAAGGGAAC 1141 GGGCCAGACG CGGGG // |
Primer: SV40pro_F_V2 Sequence file: RDB13392_A5Edb.seq ![]() |
>13392_13392_A9G8F_2_SV40pro_F_V2_G08_07_ABI08.ab1 1 TAGGAGCTTT TTTTGGGAAG GGCTTAGGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG 61 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 541 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 661 CATGGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGGCG 721 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT TGCCTGAAAG AGCTTGGCGG 781 CGAATGGGGC TGGACCCGCT TCCCTCGTGC TTTTACCGTT ATCGCCGCTC CCCGATTTCC 841 GCAACGCCAT CGCCCTTCTA TCCGCCTTCT TGACAAAGTT TCTTCTGAAG CCGGGGACTT 901 TGGGGGGTTT CGAAAATGAA CCGAACCAAA C // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP] |
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