Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-SOD1_G85R (#RDB13391)

Mammalian expression vector for human G85R SOD1 with EGFP tag at C-terminus.

Clone info. Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G85R mutation correlating familial ALS.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13391 pEGFP-SOD1_G85R DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-SOD1_G85R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13391).

Reference section:

Urushitani, M., Ezzi, S.A., Matsuo, A., Tooyama, I., Julien, J.P., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13391_A5Es.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13391_A5Esa.seq check
1
Primer: EGFP_N_rev
Sequence file: RDB13391_A5Esb.seq check
1
Primer: SV40pro_F_V2
Sequence file: RDB13391_A5Esc.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP]