Mammalian expression vector for human G85R SOD1 with EGFP tag at C-terminus.
Clone info. | Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G85R mutation correlating familial ALS. |
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Comment | Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. |
Vector backbone | pEGFP-N3 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human Superoxide dismutase 1 cDNA |
Depositor|Developer | Urushitani, Makoto | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008)) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13391 | pEGFP-SOD1_G85R | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEGFP-SOD1_G85R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13391). |
Reference section:
Urushitani, M., Ezzi, S.A., Matsuo, A., Tooyama, I., Julien, J.P., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13391_A5Es.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV-Forward Sequence file: RDB13391_A5Esa.seq ![]() |
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Primer: EGFP_N_rev Sequence file: RDB13391_A5Esb.seq ![]() |
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Primer: SV40pro_F_V2 Sequence file: RDB13391_A5Esc.seq ![]() |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP] |
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