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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-SOD1_WT (#RDB13390)

Mammalian expression vector for human SOD1 with EGFP tag at C-terminus.

Clone info. Mammalian expression vector for human WT OD1 with EGFP tag at C-terminus.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. Known differences (at least): C to T substitution at 187nt (Gly, synonym) of NM_000454.4.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13390 pEGFP-SOD1_WT DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-SOD1_WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13390).

Reference section:

Urushitani, M., Ezzi, S.A., Matsuo, A., Tooyama, I., Julien, J.P., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13390_A6Arp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Sequence file: RDB13390_A6Ara.seq check
>13390_13390_A9G8F_2_CMV-Forward_B03_06_ABI24.ab1
    1 TCCGTCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
   61 CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGAC TCAGATCTCG AGCTCAAGCT
  121 TCGAATTCGC CATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGT CCAGTGCAGG
  181 GCATCATCAA TTTCGAGCAG AAGGAAAGTA ATGGACCAGT GAAGGTGTGG GGAAGCATTA
  241 AAGGACTGAC TGAAGGCCTG CATGGATTCC ATGTTCATGA GTTTGGAGAT AATACAGCAG
  301 GCTGTACCAG TGCAGGTCCT CACTTTAATC CTCTATCCAG AAAACACGGT GGGCCAAAGG
  361 ATGAAGAGAG GCATGTTGGA GACTTGGGCA ATGTGACTGC TGACAAAGAT GGTGTGGCCG
  421 ATGTGTCTAT TGAAGATTCT GTGATCTCAC TCTCAGGAGA CCATTGCATC ATTGGCCGCA
  481 CACTGGTGGT CCATGAAAAA GCAGATGACT TGGGCAAAGG TGGAAATGAA GAAAGTACAA
  541 AGACAGGAAA CGCTGGAAGT CGTTTGGCTT GTGGTGTAAT TGGGATCGCC CAAGGATCCA
  601 TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
  661 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG
  721 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
  781 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
  841 ACATGAAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGG TCCAGGAGCG
  901 CACCATCTTC TTCAAGGGAC GACGGGCAAC TACAAGACCC GGCGCCCGAG GTGAAGTTCG
  961 AGGGCGACAC CCTGGGTGAA CCGGCATCGG AGCTGAAAGG GCATCGGACT TTCAAGGGAG
 1021 GACGGGCAAC ATCCTGGGGG CACAAGGCTG GGAAGTTACA CTACACAGGC CCACAAACGT
 1081 CTATATCATG GGCCGGACAG GCAGGGAGGG AACGGGCCAT TCCAGGTTGA ACCTTCAAGG
1141 ATTCCGGCCA CAAACCAATT CGGAAGGGAA CGGAGCGAGG GCGGAGTGTG CCATG
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB13390_A6Arb.seq check
>13390_13390_A6Ar_2_SV40pro_F_A06_03_ABI24.ab1
    1 CTTAACCATT CCAGAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG
   61 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
  121 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
  181 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
  241 CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG
  301 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
  361 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
  421 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
  481 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
  541 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
  601 TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG
  661 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC
  721 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG
  781 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT
  841 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT
  901 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG
  961 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCCGCCTG GGATGATCCT
 1021 CCAGCGCGGG GAATCTCATG CCTGGGAGTT CTTCGCCCCA CCCCTAAGGG GCAGCTAAAC
 1081 TTGAACAACG GAAAGAGAAA CAATACAGAA ATGAACCTCG CGGCCTAAGT GAACGTCAAT
1141 TAAGATAAGA ACCAGAAAGT ATCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461. [link to RRC of NBRP]