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pcDNA3-SOD1-FLAG_G93A

Mammalian expression vector for human G93A SOD1 with FLAG tag at C-terminus.

Catalog number RDB13388
Resource name pcDNA3-SOD1-FLAG_G93A
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly93Ala mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly93Ala mutation is cloned.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
Other clones in our bank
External Database
human Superoxide dismutase 1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13388 pcDNA3-SOD1-FLAG_G93A DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13388_A5Edp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,T7 pro,insert(SOD1 G93A),Flag,bGH pA
          Sequence file: RDB13388_A5Eda.seq check
          >13388_13388_A5Ed_2_CMV_Forward_G04_19.ab1
              1 CAACACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACTAGTAAC GGCCGCCAGT
            181 GTGCTGGAAT TCTGCAGAAT CATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGT
            241 CCAGTGCAGG GCATCATCAA TTTCGAGCAG AAGGAAAGTA ATGGACCAGT GAAGGTGTGG
            301 GGAAGCATTA AAGGACTGAC TGAAGGCCTG CATGGATTCC ATGTTCATGA GTTTGGAGAT
            361 AATACAGCAG GCTGTACCAG TGCAGGTCCT CACTTTAATC CTCTATCCAG AAAACACGGT
            421 GGGCCAAAGG ATGAAGAGAG GCATGTTGGA GACTTGGGCA ATGTGACTGC TGACAAAGAT
            481 GCTGTGGCCG ATGTGTCTAT TGAAGATTCT GTGATCTCAC TCTCAGGAGA CCATTGCATC
            541 ATTGGCCGCA CACTGGTGGT CCATGAAAAA GCAGATGACT TGGGCAAAGG TGGAAATGAA
            601 GAAAGTACAA AGACAGGAAA CGCTGGAAGT CGTTTGGCTT GTGGTGTAAT TGGGATCGCC
            661 CAAGACTACA AAGACGATGA CGACAAGTGA GATATCCATC ACACTGGCGG CCGCTCGAGC
            721 ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC
            781 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG
            841 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT
            901 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGG
            961 AGGATTGGGA AAGACAATAG CAGGCATGCT GGGGGATGCG GGTGGGCTCT ATGGGCTTCT
           1021 GAGGCGGGAA AGAACCCAGC TGGGGGCTCT AGGGGGTATC CCCCACGCGC CCTGTAGCCG
           1081 CGCATTAAGC GCGGCGGGCT GTGATGTAAC GCCGCAAGCG GTGACCGCTA CACTTGCCAG
          1141 GCGCCCCTTA AGCGG
          //
          Primer: SV40pro_F
          Region: Sv40 pro_ori,Neo R
          Sequence file: RDB13388_A5Edb.seq check
          >13388_13388_A5Ed_2_SV40pro_F_H04_22.ab1
              1 CCTGTAACTA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
             61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
            121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
            661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
            841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
            901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
            961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
           1021 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCCAGCGCGG GGATCTCATG CTTGGAGTCC
           1081 TCGCCCACCC CACCTTGATA TGCAGCTATA TGCTACAATA AGGCATAGCC ATCCACAAAT
          1141 TTCCACAAAA TTA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl