Mammalian expression vector for human G93A SOD1 with FLAG tag at C-terminus.
Clone info. | Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly93Ala mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly93Ala mutation is cloned. |
---|---|
Comment | Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym). |
Vector backbone | pcDNA3 (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human Superoxide dismutase 1 cDNA |
Depositor|Developer | Urushitani, Makoto | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002)) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB13388 | pcDNA3-SOD1-FLAG_G93A | DNA solution | Please contact us. |
Materials & Methods section:
The pcDNA3-SOD1-FLAG_G93A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13388). |
Reference section:
Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13388_A5Edp1-4.pdf ![]() |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert(SOD1 G93A),Flag,bGH pA Sequence file: RDB13388_A5Eda.seq ![]() |
---|
>13388_13388_A5Ed_2_CMV_Forward_G04_19.ab1 1 CAACACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACTAGTAAC GGCCGCCAGT 181 GTGCTGGAAT TCTGCAGAAT CATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGT 241 CCAGTGCAGG GCATCATCAA TTTCGAGCAG AAGGAAAGTA ATGGACCAGT GAAGGTGTGG 301 GGAAGCATTA AAGGACTGAC TGAAGGCCTG CATGGATTCC ATGTTCATGA GTTTGGAGAT 361 AATACAGCAG GCTGTACCAG TGCAGGTCCT CACTTTAATC CTCTATCCAG AAAACACGGT 421 GGGCCAAAGG ATGAAGAGAG GCATGTTGGA GACTTGGGCA ATGTGACTGC TGACAAAGAT 481 GCTGTGGCCG ATGTGTCTAT TGAAGATTCT GTGATCTCAC TCTCAGGAGA CCATTGCATC 541 ATTGGCCGCA CACTGGTGGT CCATGAAAAA GCAGATGACT TGGGCAAAGG TGGAAATGAA 601 GAAAGTACAA AGACAGGAAA CGCTGGAAGT CGTTTGGCTT GTGGTGTAAT TGGGATCGCC 661 CAAGACTACA AAGACGATGA CGACAAGTGA GATATCCATC ACACTGGCGG CCGCTCGAGC 721 ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC 781 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 841 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 901 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGG 961 AGGATTGGGA AAGACAATAG CAGGCATGCT GGGGGATGCG GGTGGGCTCT ATGGGCTTCT 1021 GAGGCGGGAA AGAACCCAGC TGGGGGCTCT AGGGGGTATC CCCCACGCGC CCTGTAGCCG 1081 CGCATTAAGC GCGGCGGGCT GTGATGTAAC GCCGCAAGCG GTGACCGCTA CACTTGCCAG 1141 GCGCCCCTTA AGCGG // |
Primer: SV40pro_F (Pr0110) Region: Sv40 pro_ori,Neo R Sequence file: RDB13388_A5Edb.seq ![]() |
>13388_13388_A5Ed_2_SV40pro_F_H04_22.ab1 1 CCTGTAACTA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT 121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC 661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC 961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA 1021 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCCAGCGCGG GGATCTCATG CTTGGAGTCC 1081 TCGCCCACCC CACCTTGATA TGCAGCTATA TGCTACAATA AGGCATAGCC ATCCACAAAT 1141 TTCCACAAAA TTA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP] |
---|
Featured content