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pcDNA3-SOD1-FLAG_G93A

Mammalian expression vector for human G93A SOD1 with FLAG tag at C-terminus.

Catalog number RDB13388
Resource name pcDNA3-SOD1-FLAG_G93A
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly93Ala mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly93Ala mutation is cloned.
Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor Urushitani, Makoto |
 
Other clones in our bank human SOD1 (NCBI Gene 6647) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
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RDB13388 pcDNA3-SOD1-FLAG_G93A DNA solution Please contact us.

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References and tips

Electronic file

Original reference

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13388_A5Edp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward RDB13388_A5Eda.seq
>13388_13388_A5Ed_2_CMV_Forward_G04_19.ab1
    1 CAACACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACTAGTAAC GGCCGCCAGT
  181 GTGCTGGAAT TCTGCAGAAT CATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGT
  241 CCAGTGCAGG GCATCATCAA TTTCGAGCAG AAGGAAAGTA ATGGACCAGT GAAGGTGTGG
  301 GGAAGCATTA AAGGACTGAC TGAAGGCCTG CATGGATTCC ATGTTCATGA GTTTGGAGAT
  361 AATACAGCAG GCTGTACCAG TGCAGGTCCT CACTTTAATC CTCTATCCAG AAAACACGGT
  421 GGGCCAAAGG ATGAAGAGAG GCATGTTGGA GACTTGGGCA ATGTGACTGC TGACAAAGAT
  481 GCTGTGGCCG ATGTGTCTAT TGAAGATTCT GTGATCTCAC TCTCAGGAGA CCATTGCATC
  541 ATTGGCCGCA CACTGGTGGT CCATGAAAAA GCAGATGACT TGGGCAAAGG TGGAAATGAA
  601 GAAAGTACAA AGACAGGAAA CGCTGGAAGT CGTTTGGCTT GTGGTGTAAT TGGGATCGCC
  661 CAAGACTACA AAGACGATGA CGACAAGTGA GATATCCATC ACACTGGCGG CCGCTCGAGC
  721 ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC
  781 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG
  841 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT
  901 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGG
  961 AGGATTGGGA AAGACAATAG CAGGCATGCT GGGGGATGCG GGTGGGCTCT ATGGGCTTCT
 1021 GAGGCGGGAA AGAACCCAGC TGGGGGCTCT AGGGGGTATC CCCCACGCGC CCTGTAGCCG
 1081 CGCATTAAGC GCGGCGGGCT GTGATGTAAC GCCGCAAGCG GTGACCGCTA CACTTGCCAG
1141 GCGCCCCTTA AGCGG
//
Primer: SV40pro_F RDB13388_A5Edb.seq
>13388_13388_A5Ed_2_SV40pro_F_H04_22.ab1
    1 CCTGTAACTA TTCAGAAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
 1021 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCCAGCGCGG GGATCTCATG CTTGGAGTCC
 1081 TCGCCCACCC CACCTTGATA TGCAGCTATA TGCTACAATA AGGCATAGCC ATCCACAAAT
1141 TTCCACAAAA TTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

2019.07.26

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