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pcDNA3-SOD1-FLAG_G93A (#RDB13388)

Mammalian expression vector for human G93A SOD1 with FLAG tag at C-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Urushitani, M., J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly93Ala mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly93Ala mutation is cloned.
Comment Expression was confirmed by the depositor with Western blotting of transfected mammalian cells using commercially available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Growth conditions 37C, LB + Amp
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。

Catalog # Resource name Shipping form Fee
RDB13388 pcDNA3-SOD1-FLAG_G93A DNA solution Please contact us.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-SOD1-FLAG_G93A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13388).

Reference section:

Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [PubMed] [Article] [RRC of NBRP]