Resource data sheet
Prev.

pcDNA3-SOD1-FLAG_G85R

Mammalian expression vector for human G85R SOD1 with FLAG tag at C-terminus.

Catalog number RDB13387
Resource name pcDNA3-SOD1-FLAG_G85R
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly85Arg mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly85Arg mutation is cloned.
Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor Urushitani, Makoto |
 
Other clones in our bank human SOD1 (NCBI Gene 6647) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13387 pcDNA3-SOD1-FLAG_G85R DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13387_A5Edp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13387_A5Eda.seq
>13387_13387_A5Ed_2_CMV_Forward_A05_02.ab1
    1 TTTATTAAAC CCCCGGCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG
  181 CCGCCAGTGT GCTGGAATTC TGCAGATATC ATGGCGACGA AGGCCGTGTG CGTGCTGAAG
  241 GGCGACGGTC CAGTGCAGGG CATCATCAAT TTCGAGCAGA AGGAAAGTAA TGGACCAGTG
  301 AAGGTGTGGG GAAGCATTAA AGGACTGACT GAAGGCCTGC ATGGATTCCA TGTTCATGAG
  361 TTTGGAGATA ATACAGCAGG CTGTACCAGT GCAGGTCCTC ACTTTAATCC TCTATCCAGA
  421 AAACACGGTG GGCCAAAGGA TGAAGAGAGG CATGTTGGAG ACTTGCGCAA TGTGACTGCT
  481 GACAAAGATG GTGTGGCCGA TGTGTCTATT GAAGATTCTG TGATCTCACT CTCAGGAGAC
  541 CATTGCATCA TTGGCCGCAC ACTGGTGGTC CATGAAAAAG CAGATGACTT GGGCAAAGGT
  601 GGAAATGAAG AAAGTACAAA GACAGGAAAC GCTGGAAGTC GTTTGGCTTG TGGTGTAATT
  661 GGGATCGCCC AAGACTACAA AGACGATGAC GACAAGTGAG ATATCCATCA CACTGGCGGC
  721 CGCTCGAGCA TGCATCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC TAGAGCTCGC
  781 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG
  841 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT
  901 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GGTGGGGGTG GGGGCAGGAC
  961 AGCAAGGGGG GAGGGATTGG GGAAAGACAA TAGCAGCATG CTGGGGGATG CGGGTGGGCT
 1021 CTATGGGCTT TCTGAGGCGG AAAGAAACCA GCTGGGGCTC TAAGGGGGTA TCCCCCACGC
 1081 GCCCTGTAGC GGCGCATAAA GCCGCGGCGA TGTGATTGTA CGGCCGCAGC GGTGACCGCT
1141 TACAACTTGC CAAGGCGCCC CTTAGCGCGT
//
Primer: SV40pro_F RDB13387_A5Edb.seq
>13387_13387_A5Ed_2_SV40pro_F_D02_11.ab1
    1 CACGTTCTTG CATCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC AAAGCGACGC
  961 CCAACCCTGC CCATCACGAG AATTTCGATT TCCACCGCCG CCTTTCTATG AATGATGGGC
 1021 CTTCGGATCG TTTTCGGGAA CGACGGCCTG GAATGAATCT CAGCGCGGCG CATCTCAATG
1081 CCTGGCAGTT TCCTTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl