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pcDNA3-SOD1-FLAG_G85R

Mammalian expression vector for human G85R SOD1 with FLAG tag at C-terminus.

Catalog number RDB13387
Resource name pcDNA3-SOD1-FLAG_G85R
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly85Arg mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly85Arg mutation is cloned.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
Other clones in our bank
External Database
human Superoxide dismutase 1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13387 pcDNA3-SOD1-FLAG_G85R DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13387_A5Edp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13387_A5Eda.seq check
          >13387_13387_A5Ed_2_CMV_Forward_A05_02.ab1
              1 TTTATTAAAC CCCCGGCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG
            181 CCGCCAGTGT GCTGGAATTC TGCAGATATC ATGGCGACGA AGGCCGTGTG CGTGCTGAAG
            241 GGCGACGGTC CAGTGCAGGG CATCATCAAT TTCGAGCAGA AGGAAAGTAA TGGACCAGTG
            301 AAGGTGTGGG GAAGCATTAA AGGACTGACT GAAGGCCTGC ATGGATTCCA TGTTCATGAG
            361 TTTGGAGATA ATACAGCAGG CTGTACCAGT GCAGGTCCTC ACTTTAATCC TCTATCCAGA
            421 AAACACGGTG GGCCAAAGGA TGAAGAGAGG CATGTTGGAG ACTTGCGCAA TGTGACTGCT
            481 GACAAAGATG GTGTGGCCGA TGTGTCTATT GAAGATTCTG TGATCTCACT CTCAGGAGAC
            541 CATTGCATCA TTGGCCGCAC ACTGGTGGTC CATGAAAAAG CAGATGACTT GGGCAAAGGT
            601 GGAAATGAAG AAAGTACAAA GACAGGAAAC GCTGGAAGTC GTTTGGCTTG TGGTGTAATT
            661 GGGATCGCCC AAGACTACAA AGACGATGAC GACAAGTGAG ATATCCATCA CACTGGCGGC
            721 CGCTCGAGCA TGCATCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC TAGAGCTCGC
            781 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG
            841 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT
            901 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GGTGGGGGTG GGGGCAGGAC
            961 AGCAAGGGGG GAGGGATTGG GGAAAGACAA TAGCAGCATG CTGGGGGATG CGGGTGGGCT
           1021 CTATGGGCTT TCTGAGGCGG AAAGAAACCA GCTGGGGCTC TAAGGGGGTA TCCCCCACGC
           1081 GCCCTGTAGC GGCGCATAAA GCCGCGGCGA TGTGATTGTA CGGCCGCAGC GGTGACCGCT
          1141 TACAACTTGC CAAGGCGCCC CTTAGCGCGT
          //
          Primer: SV40pro_F
          Sequence file: RDB13387_A5Edb.seq check
          >13387_13387_A5Ed_2_SV40pro_F_D02_11.ab1
              1 CACGTTCTTG CATCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
             61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
            121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
            661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
            781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
            841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
            901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC AAAGCGACGC
            961 CCAACCCTGC CCATCACGAG AATTTCGATT TCCACCGCCG CCTTTCTATG AATGATGGGC
           1021 CTTCGGATCG TTTTCGGGAA CGACGGCCTG GAATGAATCT CAGCGCGGCG CATCTCAATG
          1081 CCTGGCAGTT TCCTTT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl