Mammalian expression vector for human G85R SOD1 with FLAG tag at C-terminus.
Clone info. | Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly85Arg mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly85Arg mutation is cloned. |
---|---|
Comment | Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym). |
Vector backbone | pcDNA3 (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human Superoxide dismutase 1 cDNA |
Depositor|Developer | Urushitani, Makoto | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002)) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB13387 | pcDNA3-SOD1-FLAG_G85R | DNA solution | Please contact us. |
Materials & Methods section:
The pcDNA3-SOD1-FLAG_G85R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13387). |
Reference section:
Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13387_A5Edp1.pdf |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: CMV-Forward Sequence file: RDB13387_A5Eda.seq |
---|
>13387_13387_A5Ed_2_CMV_Forward_A05_02.ab1 1 TTTATTAAAC CCCCGGCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT 121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG 181 CCGCCAGTGT GCTGGAATTC TGCAGATATC ATGGCGACGA AGGCCGTGTG CGTGCTGAAG 241 GGCGACGGTC CAGTGCAGGG CATCATCAAT TTCGAGCAGA AGGAAAGTAA TGGACCAGTG 301 AAGGTGTGGG GAAGCATTAA AGGACTGACT GAAGGCCTGC ATGGATTCCA TGTTCATGAG 361 TTTGGAGATA ATACAGCAGG CTGTACCAGT GCAGGTCCTC ACTTTAATCC TCTATCCAGA 421 AAACACGGTG GGCCAAAGGA TGAAGAGAGG CATGTTGGAG ACTTGCGCAA TGTGACTGCT 481 GACAAAGATG GTGTGGCCGA TGTGTCTATT GAAGATTCTG TGATCTCACT CTCAGGAGAC 541 CATTGCATCA TTGGCCGCAC ACTGGTGGTC CATGAAAAAG CAGATGACTT GGGCAAAGGT 601 GGAAATGAAG AAAGTACAAA GACAGGAAAC GCTGGAAGTC GTTTGGCTTG TGGTGTAATT 661 GGGATCGCCC AAGACTACAA AGACGATGAC GACAAGTGAG ATATCCATCA CACTGGCGGC 721 CGCTCGAGCA TGCATCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC TAGAGCTCGC 781 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG 841 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT 901 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GGTGGGGGTG GGGGCAGGAC 961 AGCAAGGGGG GAGGGATTGG GGAAAGACAA TAGCAGCATG CTGGGGGATG CGGGTGGGCT 1021 CTATGGGCTT TCTGAGGCGG AAAGAAACCA GCTGGGGCTC TAAGGGGGTA TCCCCCACGC 1081 GCCCTGTAGC GGCGCATAAA GCCGCGGCGA TGTGATTGTA CGGCCGCAGC GGTGACCGCT 1141 TACAACTTGC CAAGGCGCCC CTTAGCGCGT // |
Primer: SV40pro_F Sequence file: RDB13387_A5Edb.seq |
>13387_13387_A5Ed_2_SV40pro_F_D02_11.ab1 1 CACGTTCTTG CATCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC AAAGCGACGC 961 CCAACCCTGC CCATCACGAG AATTTCGATT TCCACCGCCG CCTTTCTATG AATGATGGGC 1021 CTTCGGATCG TTTTCGGGAA CGACGGCCTG GAATGAATCT CAGCGCGGCG CATCTCAATG 1081 CCTGGCAGTT TCCTTT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP] |
---|
Featured content