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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-SOD1-FLAG_G85R (#RDB13387)

Mammalian expression vector for human G85R SOD1 with FLAG tag at C-terminus.

Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. Gly85Arg mutation correlating familial ALS. cDNA fragment 149 to 610nt of NM_000454.4 with Gly85Arg mutation is cloned.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13387 pcDNA3-SOD1-FLAG_G85R DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-SOD1-FLAG_G85R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13387).

Reference section:

Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13387_A5Edp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13387_A5Eda.seq check
>13387_13387_A5Ed_2_CMV_Forward_A05_02.ab1
    1 TTTATTAAAC CCCCGGCCCA ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG
  181 CCGCCAGTGT GCTGGAATTC TGCAGATATC ATGGCGACGA AGGCCGTGTG CGTGCTGAAG
  241 GGCGACGGTC CAGTGCAGGG CATCATCAAT TTCGAGCAGA AGGAAAGTAA TGGACCAGTG
  301 AAGGTGTGGG GAAGCATTAA AGGACTGACT GAAGGCCTGC ATGGATTCCA TGTTCATGAG
  361 TTTGGAGATA ATACAGCAGG CTGTACCAGT GCAGGTCCTC ACTTTAATCC TCTATCCAGA
  421 AAACACGGTG GGCCAAAGGA TGAAGAGAGG CATGTTGGAG ACTTGCGCAA TGTGACTGCT
  481 GACAAAGATG GTGTGGCCGA TGTGTCTATT GAAGATTCTG TGATCTCACT CTCAGGAGAC
  541 CATTGCATCA TTGGCCGCAC ACTGGTGGTC CATGAAAAAG CAGATGACTT GGGCAAAGGT
  601 GGAAATGAAG AAAGTACAAA GACAGGAAAC GCTGGAAGTC GTTTGGCTTG TGGTGTAATT
  661 GGGATCGCCC AAGACTACAA AGACGATGAC GACAAGTGAG ATATCCATCA CACTGGCGGC
  721 CGCTCGAGCA TGCATCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC TAGAGCTCGC
  781 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG
  841 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT
  901 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GGTGGGGGTG GGGGCAGGAC
  961 AGCAAGGGGG GAGGGATTGG GGAAAGACAA TAGCAGCATG CTGGGGGATG CGGGTGGGCT
 1021 CTATGGGCTT TCTGAGGCGG AAAGAAACCA GCTGGGGCTC TAAGGGGGTA TCCCCCACGC
 1081 GCCCTGTAGC GGCGCATAAA GCCGCGGCGA TGTGATTGTA CGGCCGCAGC GGTGACCGCT
1141 TACAACTTGC CAAGGCGCCC CTTAGCGCGT
//
Primer: SV40pro_F
Sequence file: RDB13387_A5Edb.seq check
>13387_13387_A5Ed_2_SV40pro_F_D02_11.ab1
    1 CACGTTCTTG CATCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC AAAGCGACGC
  961 CCAACCCTGC CCATCACGAG AATTTCGATT TCCACCGCCG CCTTTCTATG AATGATGGGC
 1021 CTTCGGATCG TTTTCGGGAA CGACGGCCTG GAATGAATCT CAGCGCGGCG CATCTCAATG
1081 CCTGGCAGTT TCCTTT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP]