Resource data sheet
Prev.

pcDNA3-SOD1-FLAG_WT

Mammalian expression vector for human SOD1 with FLAG tag at C-terminus.

Catalog number RDB13386
Resource name pcDNA3-SOD1-FLAG_WT
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. cDNA fragment 149 to 610nt of NM_000454.4 is cloned.
Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor Urushitani, Makoto |
 
Other clones in our bank human SOD1 (NCBI Gene 6647) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13386 pcDNA3-SOD1-FLAG_WT DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13386_A5Edp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward RDB13386_A5Eda.seq
>13386_13386_A5Ed_1_CMV_Forward_E04_13.ab1
    1 TGAACCACCT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGAGCTCG GATCCACTAG TAACGGCCGC
  181 CAGTGTGCTG GAATTCTGCA GATATCATGG CGACGAAGGC CGTGTGCGTG CTGAAGGGCG
  241 ACGGTCCAGT GCAGGGCATC ATCAATTTCG AGCAGAAGGA AAGTAATGGA CCAGTGAAGG
  301 TGTGGGGAAG CATTAAAGGA CTGACTGAAG GCCTGCATGG ATTCCATGTT CATGAGTTTG
  361 GAGATAATAC AGCAGGCTGT ACCAGTGCAG GTCCTCACTT TAATCCTCTA TCCAGAAAAC
  421 ACGGTGGGCC AAAGGATGAA GAGAGGCATG TTGGAGACTT GGGCAATGTG ACTGCTGACA
  481 AAGATGGTGT GGCCGATGTG TCTATTGAAG ATTCTGTGAT CTCACTCTCA GGAGACCATT
  541 GCATCATTGG CCGCACACTG GTGGTCCATG AAAAAGCAGA TGACTTGGGC AAAGGTGGAA
  601 ATGAAGAAAG TACAAAGACA GGAAACGCTG GAAGTCGTTT GGCTTGTGGT GTAATTGGGA
  661 TCGCCCAAGA CTACAAAGAC GATGACGACA AGTGAGATAT CCATCACACT GGCGGCCGCT
  721 CGAGCATGCA TCTAGAGGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
  961 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGGATG CGGTGGGCTC TATGGCTTCT
 1021 GAGGGCGGAA AGAAACCAGC TGGGGGCTCT AGGGGGTATC CCCCACGGCG CCCCTGTAGC
 1081 GGGCGCATTT AAGCGCGGTC GGGATGTGTG TAACGCGCAG CGTGACCGGC TTACACTTTG
1141 GTCCAAGCGC GCCCCTA
//
Primer: SV40pro_F RDB13386_A5Edb.seq
>13386_13386_A5Ed_1_SV40pro_F_F04_16.ab1
    1 CCCGCTCCTG GACCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
 1021 CGGAATCGTT TTCCGGGACG CCGGCTGATG ATCCTCAGCG CGGGGAATCT CATGCTGGAG
 1081 GTTCTTCGCC ACCCCACTGT ATGGCAGCCT ATATGTACAA ATAAGCAATA GCCATCCACC
1141 CA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

2019.07.26

GNP_filter3_RDBDEP_html_190432.pl