Mammalian expression vector for human SOD1 with FLAG tag at C-terminus.
Clone info. | Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. cDNA fragment 149 to 610nt of NM_000454.4 is cloned. |
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Comment | Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym). |
Vector backbone | pcDNA3 (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells) |
Gene/insert name | human Superoxide dismutase 1 cDNA |
Depositor|Developer | Urushitani, Makoto | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002)) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13386 | pcDNA3-SOD1-FLAG_WT | DNA solution | Please contact us. |
Materials & Methods section:
The pcDNA3-SOD1-FLAG_WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13386). |
Reference section:
Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13386_A5Edp1-4.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert,FLAG,bGH pA Sequence file: RDB13386_A5Eda.seq |
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>13386_13386_A5Ed_1_CMV_Forward_E04_13.ab1 1 TGAACCACCT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGAGCTCG GATCCACTAG TAACGGCCGC 181 CAGTGTGCTG GAATTCTGCA GATATCATGG CGACGAAGGC CGTGTGCGTG CTGAAGGGCG 241 ACGGTCCAGT GCAGGGCATC ATCAATTTCG AGCAGAAGGA AAGTAATGGA CCAGTGAAGG 301 TGTGGGGAAG CATTAAAGGA CTGACTGAAG GCCTGCATGG ATTCCATGTT CATGAGTTTG 361 GAGATAATAC AGCAGGCTGT ACCAGTGCAG GTCCTCACTT TAATCCTCTA TCCAGAAAAC 421 ACGGTGGGCC AAAGGATGAA GAGAGGCATG TTGGAGACTT GGGCAATGTG ACTGCTGACA 481 AAGATGGTGT GGCCGATGTG TCTATTGAAG ATTCTGTGAT CTCACTCTCA GGAGACCATT 541 GCATCATTGG CCGCACACTG GTGGTCCATG AAAAAGCAGA TGACTTGGGC AAAGGTGGAA 601 ATGAAGAAAG TACAAAGACA GGAAACGCTG GAAGTCGTTT GGCTTGTGGT GTAATTGGGA 661 TCGCCCAAGA CTACAAAGAC GATGACGACA AGTGAGATAT CCATCACACT GGCGGCCGCT 721 CGAGCATGCA TCTAGAGGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA GCTCGCTGAT 781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT 841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT 901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG 961 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGGATG CGGTGGGCTC TATGGCTTCT 1021 GAGGGCGGAA AGAAACCAGC TGGGGGCTCT AGGGGGTATC CCCCACGGCG CCCCTGTAGC 1081 GGGCGCATTT AAGCGCGGTC GGGATGTGTG TAACGCGCAG CGTGACCGGC TTACACTTTG 1141 GTCCAAGCGC GCCCCTA // |
Primer: SV40pro_F (Pr0110) Region: Sv40 pro_ori,Neo R Sequence file: RDB13386_A5Edb.seq |
>13386_13386_A5Ed_1_SV40pro_F_F04_16.ab1 1 CCCGCTCCTG GACCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC 121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC 961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 1021 CGGAATCGTT TTCCGGGACG CCGGCTGATG ATCCTCAGCG CGGGGAATCT CATGCTGGAG 1081 GTTCTTCGCC ACCCCACTGT ATGGCAGCCT ATATGTACAA ATAAGCAATA GCCATCCACC 1141 CA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP] |
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