Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-SOD1-FLAG_WT (#RDB13386)

Mammalian expression vector for human SOD1 with FLAG tag at C-terminus.

Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. cDNA fragment 149 to 610nt of NM_000454.4 is cloned.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13386 pcDNA3-SOD1-FLAG_WT DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-SOD1-FLAG_WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13386).

Reference section:

Urushitani, M., Kurisu, J., Tsukita, K., Takahashi, R., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13386_A5Edp1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert,FLAG,bGH pA
Sequence file: RDB13386_A5Eda.seq check
>13386_13386_A5Ed_1_CMV_Forward_E04_13.ab1
    1 TGAACCACCT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGAGCTCG GATCCACTAG TAACGGCCGC
  181 CAGTGTGCTG GAATTCTGCA GATATCATGG CGACGAAGGC CGTGTGCGTG CTGAAGGGCG
  241 ACGGTCCAGT GCAGGGCATC ATCAATTTCG AGCAGAAGGA AAGTAATGGA CCAGTGAAGG
  301 TGTGGGGAAG CATTAAAGGA CTGACTGAAG GCCTGCATGG ATTCCATGTT CATGAGTTTG
  361 GAGATAATAC AGCAGGCTGT ACCAGTGCAG GTCCTCACTT TAATCCTCTA TCCAGAAAAC
  421 ACGGTGGGCC AAAGGATGAA GAGAGGCATG TTGGAGACTT GGGCAATGTG ACTGCTGACA
  481 AAGATGGTGT GGCCGATGTG TCTATTGAAG ATTCTGTGAT CTCACTCTCA GGAGACCATT
  541 GCATCATTGG CCGCACACTG GTGGTCCATG AAAAAGCAGA TGACTTGGGC AAAGGTGGAA
  601 ATGAAGAAAG TACAAAGACA GGAAACGCTG GAAGTCGTTT GGCTTGTGGT GTAATTGGGA
  661 TCGCCCAAGA CTACAAAGAC GATGACGACA AGTGAGATAT CCATCACACT GGCGGCCGCT
  721 CGAGCATGCA TCTAGAGGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
  961 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGGATG CGGTGGGCTC TATGGCTTCT
 1021 GAGGGCGGAA AGAAACCAGC TGGGGGCTCT AGGGGGTATC CCCCACGGCG CCCCTGTAGC
 1081 GGGCGCATTT AAGCGCGGTC GGGATGTGTG TAACGCGCAG CGTGACCGGC TTACACTTTG
1141 GTCCAAGCGC GCCCCTA
//
Primer: SV40pro_F (Pr0110)
Region: Sv40 pro_ori,Neo R
Sequence file: RDB13386_A5Edb.seq check
>13386_13386_A5Ed_1_SV40pro_F_F04_16.ab1
    1 CCCGCTCCTG GACCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
 1021 CGGAATCGTT TTCCGGGACG CCGGCTGATG ATCCTCAGCG CGGGGAATCT CATGCTGGAG
 1081 GTTCTTCGCC ACCCCACTGT ATGGCAGCCT ATATGTACAA ATAAGCAATA GCCATCCACC
1141 CA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574. [link to RRC of NBRP]