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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-SOD1-FLAG_WT

Mammalian expression vector for human SOD1 with FLAG tag at C-terminus.

Catalog number RDB13386
Resource name pcDNA3-SOD1-FLAG_WT
Clone info. Mammalian expression vector for human SOD1 with FLAG tag at C-terminus. cDNA fragment 149 to 610nt of NM_000454.4 is cloned.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-FLAG antibodies. Known differences (at least): C to T substitution at 187nt (Gly, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
Other clones in our bank
External Database
human Superoxide dismutase 1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Neurochem., 83(5), 1030-1042 (2002))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Neurochem., 83(5), 1030-1042 (2002))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13386 pcDNA3-SOD1-FLAG_WT DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13386_A5Edp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,T7 pro,insert,FLAG,bGH pA
          Sequence file: RDB13386_A5Eda.seq check
          >13386_13386_A5Ed_1_CMV_Forward_E04_13.ab1
              1 TGAACCACCT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGAGCTCG GATCCACTAG TAACGGCCGC
            181 CAGTGTGCTG GAATTCTGCA GATATCATGG CGACGAAGGC CGTGTGCGTG CTGAAGGGCG
            241 ACGGTCCAGT GCAGGGCATC ATCAATTTCG AGCAGAAGGA AAGTAATGGA CCAGTGAAGG
            301 TGTGGGGAAG CATTAAAGGA CTGACTGAAG GCCTGCATGG ATTCCATGTT CATGAGTTTG
            361 GAGATAATAC AGCAGGCTGT ACCAGTGCAG GTCCTCACTT TAATCCTCTA TCCAGAAAAC
            421 ACGGTGGGCC AAAGGATGAA GAGAGGCATG TTGGAGACTT GGGCAATGTG ACTGCTGACA
            481 AAGATGGTGT GGCCGATGTG TCTATTGAAG ATTCTGTGAT CTCACTCTCA GGAGACCATT
            541 GCATCATTGG CCGCACACTG GTGGTCCATG AAAAAGCAGA TGACTTGGGC AAAGGTGGAA
            601 ATGAAGAAAG TACAAAGACA GGAAACGCTG GAAGTCGTTT GGCTTGTGGT GTAATTGGGA
            661 TCGCCCAAGA CTACAAAGAC GATGACGACA AGTGAGATAT CCATCACACT GGCGGCCGCT
            721 CGAGCATGCA TCTAGAGGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA GCTCGCTGAT
            781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
            841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
            901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
            961 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGGATG CGGTGGGCTC TATGGCTTCT
           1021 GAGGGCGGAA AGAAACCAGC TGGGGGCTCT AGGGGGTATC CCCCACGGCG CCCCTGTAGC
           1081 GGGCGCATTT AAGCGCGGTC GGGATGTGTG TAACGCGCAG CGTGACCGGC TTACACTTTG
          1141 GTCCAAGCGC GCCCCTA
          //
          Primer: SV40pro_F
          Region: Sv40 pro_ori,Neo R
          Sequence file: RDB13386_A5Edb.seq check
          >13386_13386_A5Ed_1_SV40pro_F_F04_16.ab1
              1 CCCGCTCCTG GACCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
            661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCG ACCAAGCGAC
            961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
           1021 CGGAATCGTT TTCCGGGACG CCGGCTGATG ATCCTCAGCG CGGGGAATCT CATGCTGGAG
           1081 GTTCTTCGCC ACCCCACTGT ATGGCAGCCT ATATGTACAA ATAAGCAATA GCCATCCACC
          1141 CA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Urushitani, M., Proteasomal inhibition by misfolded mutant superoxide dismutase 1 induces selective motor neuron death in familial amyotrophic lateral sclerosis. J. Neurochem. 83 (5): 1030-1042 (2002). PMID 12437574.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl