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pCNL-C1_fibrillarin

Cyan Nano-lantern for labeling of nucleoli by the human fibrillarin.

Catalog number RDB13369
Resource name pCNL-C1_fibrillarin
Clone info. Expression vector of the Cyan Nano-lantern for labeling of nucleoli by the human fibrillarin.
Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name human fibrillarin cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
 
Other clones in our bank human FBL (NCBI Gene 2091) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13369 pCNL-C1_fibrillarin Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Nano-lantern (English text)
Featured content Nano-lantern (Japanese text)
Featured content Visualization of Organelles (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2021.03.26

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