RDB13345 - pCNL-N1

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pCNL-N1_LAMP1 (#RDB13345)

Cyan Nano-lantern for labeling of lysosome by the rat LAMP1.

Clone info.	Expression vector of the Cyan Nano-lantern for labeling of lysosome by the rat LAMP1. LAMP1 cDNA fragment 104 to 1324nt of NM_012857.2 is cloned.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam. Known difference; substitutions of T to G at 253nt (Asp to Glu), T to C at 715nt (Leu, synonym), respectively.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Gene/insert name	rat LAMP1 cDNA
Depositor\|Developer	Okada, Yasushi \| Takai, Akira \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13345	pCNL-N1_LAMP1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCNL-N1_LAMP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13345).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13345_A5E8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13345_A5E8a.seq
>13345_13345_A5E8_1_CMV-Forward_C03_09.ab1 1 ATTTAAACTC CGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG 121 CTCAAGCTTC GAATTCTCGC CACCATGGCG GCCCCGGGCG CCCGGCGGCC GCTGCTCCTG 181 TTGCTGCTGG CAGGCCTTGC ACACAGCGCC CCAGCACTGT TCGAGGTGAA AGACAACAAC 241 GGCACAGCGT GTATAATGGC CAGCTTCTCT GCCTCCTTTC TGACCACCTA TGAGGCTGGA 301 CATGTTTCTA AGGTCTCGAA TATGACCCTG CCAGCCTCTG CAGAAGTCCT GAAGAATAGC 361 AGCTCTTGTG GTGAAAAGAA TGCTTCTGAG CCCACCCTCG CAATCACCTT TGGAGAAGGA 421 TATTTACTGA AACTCACCTT CACAAAAAAC ACAACACGTT ACAGTGTCCA GCACATGTAT 481 TTCACATATA ACCTGTCAGA CACACAATTC TTTCCCAATG CCAGCTCCAA AGGGCCCGAC 541 ACTGTGGATT CCACAACTGA CATCAAGGCA GACATCAACA AAACATACCG ATGTGTCAGC 601 GACATCAGGG TCTACATGAA GAATGTGACC ATTGTGCTCT GGGACGCTAC TATCCAGGCC 661 TACCTGCCGA GTAGCAACTT CAGCAAGGAA GAGACACGCT GCCCACAGGA TCAACCTTCC 721 CCAACTACTG GGCCACCCAG CCCCTCACCA CCACTCGTGC CCACAAACCC CAGTGTGTCC 781 AAGTACAATG TGACTGGTGA CAATGGAACC TGCCTGCTGG CCTCTATGGC ACTGCAACTC 841 AACATCACCT ACATGAAGAA GGACAACACG ACTGTGACCA GAGCATTCAA CATCAACCCA 901 AGTGACAAAT ATAGTGGGAC TTGCGGTGCC CAGTTGGTGA CCCTGAAAGG TGGGGGAACA 961 AGAGCAGAGT CCTGGTAGCT GCAGTTTTGG GGATGAAATG CCCACTTTCT AGCCCTGTTT 1021 TTTTCCCTGC AAGGAGGTTT CAGTTTGACA TGACTCTTTC ATGGATGGCA TAGAGCCCAC 1081 GATCAGCACC TTCCGAACTA TCCCTTGAAA GGCTTCGTTC CAGGACCCAG ATG //
Primer: YFP_R Sequence file: RDB13345_A5E8b.seq
>13345_13345_1_YFP_R_G10_19.ab1 1 AGTATGCATG ACTCGCGGAA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC 61 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG TGGCGACCGG 121 TGGATCCCGG ATGGTCTGAT AGCCCGCGTG ACTCCTCTTC CTGCCGATGA GGTAGGCGAT 181 GAGGACGATG AGGACCAGCC CTGCCAGGGC CCCGCCCACA GCAATGGGGA TCAGCATGTT 241 GTTACCGTCC TGTACACACT CTTCCACAGA CCCAAACCTG TCACTTTCTA CCCTGAAAGC 301 CTGGACTTGC ACGCTGAAGA CATTGAGGGC GAGCGCCTTG CTGACAAAGA TGTGCTCCTC 361 ACTGTTGCAC TTGTATGAGT TGCCGACACT GGCCTGAAGA GCTTTCAGGG AATAGTTGGA 421 GGTGCTGAAC GTGGGCTCTA TGGCATCAGG AAGAGTCATG TTCAACTGAA CTCCTTGCAG 481 GAAAAACAGG CTAGAAGTGG CATTCATCCC AAACTGCAGC TCCAGGACTC TGCTCTTGTT 541 CCCCACCTTC AGGGTCACCA ACTGGGCACC GCAAGTCCCA CTATATTTGT CACTTGGGTT 601 GATGTTGAAT GCTCTGGTCA CAGTCGTGTT GTCCTTCTTC ATGTAGGTGA TGTTGAGTTG 661 CAGTGCCATA GAGGCCAGCA GGCAGGTTCC ATTGTCACCA GTCACATTGT ACTTGGACAC 721 ACTGGGGTTT GTGGGCACGA GTGGTGGTGA GGGGCTGGGT GGCCCAGTAG TTGGGGAAGG 781 TTGATCCTGT GGGCAGCGTG TCTCTTCCTT GCTGAAGTTG CTACTCGGCA GGTAGGCCTG 841 GATAGTAGCG TCCCAGAGCA CAATGGTCAC ATTCTTCATG TAGACCCTGA TGTCGCTGAC 901 ACATCGGTAT GTTTTGTTGA TGTCTGCCTT GATGTCAGTT GTGGAATCCA CAGTGTCGGG 961 CCCTTTGGAG CTGGCATTGG GAAAGAATTG TGTGTCTGAC AGGTTATATG TGAAATACAT 1021 GTGCTGGACA CTGTAACGTG TGTGTTTTTT GTGATGTGAG TTCAGTAAAT ATCTTCTCAA 1081 AGTTGATGCG AGGTGGGCTC AGAGCATCTT TCACTACAAG AGCTGCTATC CTCAAGACCT 1141 CTGGCCAGAA GCTGGCCAGG G //
Primer: EGFP-C_rev Sequence file: RDB13345_A5E8c.seq
>13345_13345_A5E8_1_EGFP_C_rev_A01_01.ab1 1 AGCATCGATG GGGTCTTTGC TCAGCTTGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG 61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC 121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC 181 GGCGGTGATA TAGACGTTGT CGCTAAAGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT 241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC 301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC 361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG 421 GTAGCGGGCG AAGCACTGCA CGCCCCAGGA CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC 481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC 541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG 601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTGG 661 ATCCCGGATG GTCTGATAGC CCGCGTGACT CCTCTTCCTG CCGATGAGGT AGGCGATGAG 721 GACGATGAGG ACCAGCCCTG CCAGGGCCCC GCCCACAGCA ATGGGGATCA GCATGTTGTT 781 ACCGTCCTGT ACACACTCTT CCACAGACCC AAACCTGTCA CTTTCTACCC TGAAAGCCTG 841 GACTTGCACG CTGAAGACAT TGAGGGCGAG CGCCTTGCTG ACAAAGATGT GCTCCTCACT 901 GTTGCACTTG TATGAGTTGC CGACACTGGC CTGAAGAGCT TTCAGGGAAA TAGTTGGAGG 961 TGCTGAACGT GGGCTCTATG GCATCAGGAA GAGTCATGTT CAACTGAACT CCTTTGCAGG 1021 AAAAACAGGC TAGAAGTGGC ATTCATCCCA AACTGCAGCT CCAGGACTCT GCTTCTGTCC 1081 TCACTTTCAG GTCACAACTG GCACGGCAGG TTCCACTATA TTTTCACTTG GGTTGATGTT 1141 TGGCAT //
Primer: ME-1290Rv Sequence file: RDB13345_A5E8d.seq
>13345_13345_1_ME-1290RV_A11_02.ab1 1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT 61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA GTCGCGGCCG CCTACTGCTC 121 GTTCTTCAGC ACTCTCTCCA CGAAGCTCTT GATGTACTTG CCCATCTCGT CGGGGGCGTC 181 CTCCTGGAGG AAGTGCAGGC CCTTCACCTT CACGAACTCG GTGTTGGGGA ACTTCTTGGC 241 GCCCTCCACG ATGGCGTTGC TGAAGAAGCC GGGGTCGCCC TCGATGAACA GCTTGGGCAG 301 GTCGTCGCTG GCTCTCAGGT AGGCGTTGTA GTTTCTCACG ATCTGCACCA CGTCGGGCTT 361 GCCGCCCTTC ACCAGGGGGA TCTCTCTGGG CCAGCTCAGG GTGGGTCTTC TCACCTCGCC 421 CTTCTCCTTG AAGGGCTCCA GGTAGGCGGC GAACTCCTCG GGCTCCAGCT TTCTCATGAT 481 CTTGCTGGGC AGCACGGTCT CCACGAAGAA GTTGTTCTCC AGCACCATCT TCTCGCCCTC 541 CTCGCTCTTG ATCAGGGCGA TGTCCTCCTC GATGTCTGGC CACTCGTCCC AGCTCTCGAT 601 CACGTCCACC ACGCTCTCCA TGTGCACGAT GGCCTTGATC CTGTCCTGGT GCTCGTAGGC 661 GTAGTGGAAG GCCAGGGCGG CGCCCCAGTC GTGGCCCACG AAGATGATCT TCTTGGGCAG 721 GTTCAGAAGC TCGAACCAGG CGGTCAGGTA CTTGTAGTGG TCCAGCAGCC TGTAGCTGCC 781 GTTGCCGCTC TTGCCGCTCT TGCCCATGCC GATCAGATCG GGGGATGATG CACCTGGCCA 841 CGGGCTCGAT GTGGGGCACC ACGTGCCTCC ACAGGTAGCT GCTAGTGGCG TTGCCGTGCA 901 GGAAGATCAC GGCGTTCTCG GCGTGCTTCT CGCTGTCGTA GTAGTTGATG AAGCTGTCCA 961 GCACGTTCAT CTGCTTGCAC CTGGCCCACC ACTGGGGGGC CGGTGATCAT CCCTCTTTCT 1021 CTGCTCGGGG TCGTACACCT TTGGTACCCC CGGCGGCGGT CACGACTCAG CAGGACATGT 1081 GAATCGCGCT TCTCGTGGGG TCTTTGCTCA GCTGACTGCT GCTCAAGATA GTGATGTCGG 1141 CCAGCCAGCC CACGGGGCCC GATCCGCCCC GA //
Primer: SV40pro_F_V2 Sequence file: RDB13345_A5E8e.seq
>13345_13345_SV40pro_F_V2_F03_18_ABI24.ab1 1 ACCAAACTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG 61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 961 GAAATCGTTT TCCGGGACGC CGGCTGGGAT GATCCCTCCA GCGCGGGGAT CTCATGCTGG 1021 AGTTCTTCGC CCACCCTAGG GGAGGCTAAC TGAAACACGG GAAAGAGACA TACCGGCAGG 1081 AACCCGCGGC TATGACCGCC AATTAAAGAC GGAATAAAAC GCCACCGGGT TGTTTGGGGA 1141 TCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]

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rat LAMP1 and ortholog

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rat LAMP1

Reference sequence