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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCNL-N1_LAMP1

Cyan Nano-lantern for labeling of lysosome by the rat LAMP1.

Catalog number RDB13345
Resource name pCNL-N1_LAMP1
Clone info. Expression vector of the Cyan Nano-lantern for labeling of lysosome by the rat LAMP1. LAMP1 cDNA fragment 104 to 1324nt of NM_012857.2 is cloned.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam. Known difference; substitutions of T to G at 253nt (Asp to Glu), T to C at 715nt (Leu, synonym), respectively.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name rat LAMP1 cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
Other clones in our bank

External Database
rat LAMP1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13345 pCNL-N1_LAMP1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Nano-lantern (English text)
          Featured content Nano-lantern (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Lysosome (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13345_A5E8p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13345_A5E8a.seq check
          >13345_13345_A5E8_1_CMV-Forward_C03_09.ab1
              1 ATTTAAACTC CGCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG
            121 CTCAAGCTTC GAATTCTCGC CACCATGGCG GCCCCGGGCG CCCGGCGGCC GCTGCTCCTG
            181 TTGCTGCTGG CAGGCCTTGC ACACAGCGCC CCAGCACTGT TCGAGGTGAA AGACAACAAC
            241 GGCACAGCGT GTATAATGGC CAGCTTCTCT GCCTCCTTTC TGACCACCTA TGAGGCTGGA
            301 CATGTTTCTA AGGTCTCGAA TATGACCCTG CCAGCCTCTG CAGAAGTCCT GAAGAATAGC
            361 AGCTCTTGTG GTGAAAAGAA TGCTTCTGAG CCCACCCTCG CAATCACCTT TGGAGAAGGA
            421 TATTTACTGA AACTCACCTT CACAAAAAAC ACAACACGTT ACAGTGTCCA GCACATGTAT
            481 TTCACATATA ACCTGTCAGA CACACAATTC TTTCCCAATG CCAGCTCCAA AGGGCCCGAC
            541 ACTGTGGATT CCACAACTGA CATCAAGGCA GACATCAACA AAACATACCG ATGTGTCAGC
            601 GACATCAGGG TCTACATGAA GAATGTGACC ATTGTGCTCT GGGACGCTAC TATCCAGGCC
            661 TACCTGCCGA GTAGCAACTT CAGCAAGGAA GAGACACGCT GCCCACAGGA TCAACCTTCC
            721 CCAACTACTG GGCCACCCAG CCCCTCACCA CCACTCGTGC CCACAAACCC CAGTGTGTCC
            781 AAGTACAATG TGACTGGTGA CAATGGAACC TGCCTGCTGG CCTCTATGGC ACTGCAACTC
            841 AACATCACCT ACATGAAGAA GGACAACACG ACTGTGACCA GAGCATTCAA CATCAACCCA
            901 AGTGACAAAT ATAGTGGGAC TTGCGGTGCC CAGTTGGTGA CCCTGAAAGG TGGGGGAACA
            961 AGAGCAGAGT CCTGGTAGCT GCAGTTTTGG GGATGAAATG CCCACTTTCT AGCCCTGTTT
           1021 TTTTCCCTGC AAGGAGGTTT CAGTTTGACA TGACTCTTTC ATGGATGGCA TAGAGCCCAC
          1081 GATCAGCACC TTCCGAACTA TCCCTTGAAA GGCTTCGTTC CAGGACCCAG ATG
          //
          Primer: YFP_R
          Sequence file: RDB13345_A5E8b.seq check
          >13345_13345_1_YFP_R_G10_19.ab1
              1 AGTATGCATG ACTCGCGGAA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
             61 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG TGGCGACCGG
            121 TGGATCCCGG ATGGTCTGAT AGCCCGCGTG ACTCCTCTTC CTGCCGATGA GGTAGGCGAT
            181 GAGGACGATG AGGACCAGCC CTGCCAGGGC CCCGCCCACA GCAATGGGGA TCAGCATGTT
            241 GTTACCGTCC TGTACACACT CTTCCACAGA CCCAAACCTG TCACTTTCTA CCCTGAAAGC
            301 CTGGACTTGC ACGCTGAAGA CATTGAGGGC GAGCGCCTTG CTGACAAAGA TGTGCTCCTC
            361 ACTGTTGCAC TTGTATGAGT TGCCGACACT GGCCTGAAGA GCTTTCAGGG AATAGTTGGA
            421 GGTGCTGAAC GTGGGCTCTA TGGCATCAGG AAGAGTCATG TTCAACTGAA CTCCTTGCAG
            481 GAAAAACAGG CTAGAAGTGG CATTCATCCC AAACTGCAGC TCCAGGACTC TGCTCTTGTT
            541 CCCCACCTTC AGGGTCACCA ACTGGGCACC GCAAGTCCCA CTATATTTGT CACTTGGGTT
            601 GATGTTGAAT GCTCTGGTCA CAGTCGTGTT GTCCTTCTTC ATGTAGGTGA TGTTGAGTTG
            661 CAGTGCCATA GAGGCCAGCA GGCAGGTTCC ATTGTCACCA GTCACATTGT ACTTGGACAC
            721 ACTGGGGTTT GTGGGCACGA GTGGTGGTGA GGGGCTGGGT GGCCCAGTAG TTGGGGAAGG
            781 TTGATCCTGT GGGCAGCGTG TCTCTTCCTT GCTGAAGTTG CTACTCGGCA GGTAGGCCTG
            841 GATAGTAGCG TCCCAGAGCA CAATGGTCAC ATTCTTCATG TAGACCCTGA TGTCGCTGAC
            901 ACATCGGTAT GTTTTGTTGA TGTCTGCCTT GATGTCAGTT GTGGAATCCA CAGTGTCGGG
            961 CCCTTTGGAG CTGGCATTGG GAAAGAATTG TGTGTCTGAC AGGTTATATG TGAAATACAT
           1021 GTGCTGGACA CTGTAACGTG TGTGTTTTTT GTGATGTGAG TTCAGTAAAT ATCTTCTCAA
           1081 AGTTGATGCG AGGTGGGCTC AGAGCATCTT TCACTACAAG AGCTGCTATC CTCAAGACCT
          1141 CTGGCCAGAA GCTGGCCAGG G
          //
          Primer: EGFP-C_rev
          Sequence file: RDB13345_A5E8c.seq check
          >13345_13345_A5E8_1_EGFP_C_rev_A01_01.ab1
              1 AGCATCGATG GGGTCTTTGC TCAGCTTGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
             61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC
            121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC
            181 GGCGGTGATA TAGACGTTGT CGCTAAAGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
            241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
            301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
            361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
            421 GTAGCGGGCG AAGCACTGCA CGCCCCAGGA CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
            481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
            541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
            601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTGG
            661 ATCCCGGATG GTCTGATAGC CCGCGTGACT CCTCTTCCTG CCGATGAGGT AGGCGATGAG
            721 GACGATGAGG ACCAGCCCTG CCAGGGCCCC GCCCACAGCA ATGGGGATCA GCATGTTGTT
            781 ACCGTCCTGT ACACACTCTT CCACAGACCC AAACCTGTCA CTTTCTACCC TGAAAGCCTG
            841 GACTTGCACG CTGAAGACAT TGAGGGCGAG CGCCTTGCTG ACAAAGATGT GCTCCTCACT
            901 GTTGCACTTG TATGAGTTGC CGACACTGGC CTGAAGAGCT TTCAGGGAAA TAGTTGGAGG
            961 TGCTGAACGT GGGCTCTATG GCATCAGGAA GAGTCATGTT CAACTGAACT CCTTTGCAGG
           1021 AAAAACAGGC TAGAAGTGGC ATTCATCCCA AACTGCAGCT CCAGGACTCT GCTTCTGTCC
           1081 TCACTTTCAG GTCACAACTG GCACGGCAGG TTCCACTATA TTTTCACTTG GGTTGATGTT
          1141 TGGCAT
          //
          Primer: ME-1290Rv
          Sequence file: RDB13345_A5E8d.seq check
          >13345_13345_1_ME-1290RV_A11_02.ab1
              1 ATGCAATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT
             61 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTAGA GTCGCGGCCG CCTACTGCTC
            121 GTTCTTCAGC ACTCTCTCCA CGAAGCTCTT GATGTACTTG CCCATCTCGT CGGGGGCGTC
            181 CTCCTGGAGG AAGTGCAGGC CCTTCACCTT CACGAACTCG GTGTTGGGGA ACTTCTTGGC
            241 GCCCTCCACG ATGGCGTTGC TGAAGAAGCC GGGGTCGCCC TCGATGAACA GCTTGGGCAG
            301 GTCGTCGCTG GCTCTCAGGT AGGCGTTGTA GTTTCTCACG ATCTGCACCA CGTCGGGCTT
            361 GCCGCCCTTC ACCAGGGGGA TCTCTCTGGG CCAGCTCAGG GTGGGTCTTC TCACCTCGCC
            421 CTTCTCCTTG AAGGGCTCCA GGTAGGCGGC GAACTCCTCG GGCTCCAGCT TTCTCATGAT
            481 CTTGCTGGGC AGCACGGTCT CCACGAAGAA GTTGTTCTCC AGCACCATCT TCTCGCCCTC
            541 CTCGCTCTTG ATCAGGGCGA TGTCCTCCTC GATGTCTGGC CACTCGTCCC AGCTCTCGAT
            601 CACGTCCACC ACGCTCTCCA TGTGCACGAT GGCCTTGATC CTGTCCTGGT GCTCGTAGGC
            661 GTAGTGGAAG GCCAGGGCGG CGCCCCAGTC GTGGCCCACG AAGATGATCT TCTTGGGCAG
            721 GTTCAGAAGC TCGAACCAGG CGGTCAGGTA CTTGTAGTGG TCCAGCAGCC TGTAGCTGCC
            781 GTTGCCGCTC TTGCCGCTCT TGCCCATGCC GATCAGATCG GGGGATGATG CACCTGGCCA
            841 CGGGCTCGAT GTGGGGCACC ACGTGCCTCC ACAGGTAGCT GCTAGTGGCG TTGCCGTGCA
            901 GGAAGATCAC GGCGTTCTCG GCGTGCTTCT CGCTGTCGTA GTAGTTGATG AAGCTGTCCA
            961 GCACGTTCAT CTGCTTGCAC CTGGCCCACC ACTGGGGGGC CGGTGATCAT CCCTCTTTCT
           1021 CTGCTCGGGG TCGTACACCT TTGGTACCCC CGGCGGCGGT CACGACTCAG CAGGACATGT
           1081 GAATCGCGCT TCTCGTGGGG TCTTTGCTCA GCTGACTGCT GCTCAAGATA GTGATGTCGG
          1141 CCAGCCAGCC CACGGGGCCC GATCCGCCCC GA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13345_A5E8e.seq check
          >13345_13345_SV40pro_F_V2_F03_18_ABI24.ab1
              1 ACCAAACTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
            721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
            841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
            901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
            961 GAAATCGTTT TCCGGGACGC CGGCTGGGAT GATCCCTCCA GCGCGGGGAT CTCATGCTGG
           1021 AGTTCTTCGC CCACCCTAGG GGAGGCTAAC TGAAACACGG GAAAGAGACA TACCGGCAGG
           1081 AACCCGCGGC TATGACCGCC AATTAAAGAC GGAATAAAAC GCCACCGGGT TGTTTGGGGA
          1141 TCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
          reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl