RDB13331 - pONL-N1

Resource data sheet
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pONL-N1_Mito

Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.


Catalog number	RDB13331
Resource name	pONL-N1_Mito
Clone info.	Expression vector of the Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Gene/insert name	human cytochrome c oxidase subunit VIII cDNA
Depositor	Okada, Yasushi \| Takai, Akira \|
Other clones in our bank	human COX8A (NCBI Gene 1351) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki

Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13331	pONL-N1_Mito	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content	Nano-lantern (English text)
Featured content	Nano-lantern (Japanese text)
Featured content	Organelle marker (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

2019.04.09

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