Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.
Catalog number | RDB13331 |
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Resource name | pONL-N1_Mito |
Clone info. | Expression vector of the Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI. |
Vector backbone | pEGFP-N1 (Plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kan^r (E. coli) |
Gene/insert name | human cytochrome c oxidase subunit VIII cDNA |
Depositor | Okada, Yasushi | Takai, Akira | |
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Other clones in our bank | human COX8A (NCBI Gene 1351) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS. |
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Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13331 | pONL-N1_Mito | DNA solution |
Electronic file
Featured content
Featured content | Nano-lantern (English text) |
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Featured content | Nano-lantern (Japanese text) |
Featured content | Organelle marker (English text) |
References
original | Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. |
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User reports and related articles (go to bottom)
This clone will be sequenced a portion and digested by restriction enzyme for examination.
References
2019.04.09
GNP_filter3_RDBDEP_html_190327.pl