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PB111-hMyoD (#RDB13320)

Tetracycline inducible MyoD expression piggyBac vector

Clone info. Tetracycline inducible MyoD expression piggyBac vector. A mCherry expression can be detected in accord with MyoD expression when tetracycline is added.
Vector backbone PB-TAC-ERN (RDB13243) (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Growth conditions 37oC, LB+Amp
Gene/insert name Human MyoD cDNA
Depositor|Developer CiRA, Kyoto Univ. | Sakurai, Hidetoshi |
 
Sequence (full) RDB13320hts01.seq checkAssembled from experimentally sequenced data.
Publication Tanaka, A., PLoS One 8 (4): e61540 (2013). PMID 23626698. [link to Journal]
Test sheet RDB13320C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Approval form by depositor [Word]
This RESOURCE contains a piggyBac® transposon. Limited Use License Agreement (LULA) [Word] is also required.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution For provision of this material, prior permission by the Depositor (Kyoto University cira-keiyaku@cira.kyoto-u.ac.jp) is required. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Tanaka A et al., PLoS One 8, 2013, e61540). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
Remarks An additional fee for license fee of Gateway(R) Expression Vector is required. An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
Please obtain written approval of the depositor.
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提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
寄託者による提供承諾書 [Word]
piggyBac®トランスポゾンを内包するバイオリソースです。Limited Use License Agreement (LULA) [Word]の提出をお願いします。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、事前に寄託者 (連絡先：京都大学 cira-keiyaku@cira.kyoto-u.ac.jp) から提供承諾を得る。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Tanaka A et al., PLoS One 8, 2013, e61540)。学術機関の学術研究に限る。
備考 Gateway(R)発現ベクター。ライセンス料相当額を負担して頂きます。クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
寄託者の書面による提供承諾が必要です。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13320 PB111-hMyoD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The PB111-hMyoD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13320).

Reference section:

Tanaka, A., Woltjen, K., Miyake, K., Hotta, A., Ikeya, M., Yamamoto, T., Nishino, T., Shoji, E., Sehara-Fujisawa, A., Manabe, Y., Fujii, N., Hanaoka, K., Era, T., Yamashita, S., Isobe, K., Kimura, E., Sakurai, H., Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro. PLoS One 8 (4): e61540 (2013). PMID 23626698. [link to Journal]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tanaka, A., Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro. PLoS One 8 (4): e61540 (2013). PMID 23626698. [link to Journal]
protocol Kim, S.I., Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol. Biol. 1357: 111-131 (2016). PMID 26025620. [link to RRC of NBRP]