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pUC-T7-EMCV-GST-CysRS

EMCV IRES-dependent expression vector of human cysteinyl-tRNA synthetase

Catalog number RDB13275
Resource name pUC-T7-EMCV-GST-CysRS
Clone info. EMCV IRES-dependent expression vector of human cysteinyl-tRNA synthetase.
Comment Expression was confirmed by depositor with CBB staining.
Vector backbone pUC-T7-EMCV-GST (Plasmid)
Size of vector backbone 4.5 kb
Selectable markers Amp^r
Gene/insert name Human cycteinyl -tRNA synthetase cDNA
Depositor|Developer Imataka, Hiroaki |
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External Database
Human cycteinyl -tRNA synthetase
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB13275 pUC-T7-EMCV-GST-CysRS Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
          reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

          2022.05.17

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