Sequencing and PCR primers for primer information.
Expression vector of human eRF3
| Catalog number | RDB13270 |
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| Resource name | pGEX6P-eRF3 |
| Clone info. | Expression vector of human eRF3. Expression was confirmed by depositor with Western blot and CBB staining. cDNA fragment 663..2162 of NM_002094.3 is cloned. Known difference (at least): C to G substitution at 2,003nt (Val, synonym), A to G substitution at 2,081nt (Val, synonym). |
| Vector backbone | pGEX6P (Plasmid) |
| Size of vector backbone | 5.0 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Human eRF3 cDNA |
| Depositor | Imataka, Hiroaki | |
| Other clones in our bank | human GSPT1 (NCBI Gene 2935) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014) |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13270 | pGEX6P-eRF3 | DNA solution | Please contact us. |
Electronic file
Featured content
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13270_A7Drp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pGEX-F2 | RDB13270_A7Dra.seq |
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>13270_13270_A7Dr_pGEX-F2_D10_10_ABI24.ab1 1 GTAGTAGTTT GTTTTAAAAA CGTATTGAGC TATCCCACAA ATTGATAAGT ACTTGAAATC 61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA 121 TCCTCCAAAA TCGGATCTGG AAGTTCTGTT CCAGGGGCCC CTGGGATCCC CGGAATTCAT 181 GGAACTTTCA GAACCTATTG TAGAAAATGG AGAGACAGAA ATGTCTCCAG AAGAATCATG 241 GGAGCACAAA GAAGAAATAA GTGAAGCAGA GCCAGGGGGT GGTTCCTTGG GAGATGGAAG 301 GCCGCCAGAG GAAAGTGCCC ATGAAATGAT GGAGGAGGAA GAGGAAATCC CAAAACCTAA 361 GTCTGTGGTT GCACCGCCAG GTGCTCCTAA GAAAGAGCAT GTAAATGTAG TATTCATTGG 421 GCACGTAGAT GCTGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT 481 TGACAAAAGG ACGCTTGAAA AGTATGAAAG AGAAGCTAAA GAGAAAAACA GAGAAACTTG 541 GTACTTGTCT TGGGCCTTAG ACACAAATCA GGAAGAACGA GACAAGGGTA AAACAGTAGA 601 AGTGGGTCGT GCCTATTTTG AAACCGAAAA GAAGCATTTC ACAATTCTAG ATGCCCCTGG 661 CCACAAGAGT TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGATT TGGCTGTGCT 721 GGTAATCTCA GCCAGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG 781 AGAACATGCA ATGTTGGCAA AGACAGCAGG TGTAAAACAC CTAATTGTGC TAATTAATAA 841 GATGGATGAT CCAACAGTAA ATTGGAGCAA TGAGAGATAT GAAGAATGTA AGGAGAAACT 901 AGTGCCATTT TTGAAAAAAG TTGGCTTCAA TCCCAAAAAG GACATTCACT TTATGCCCTG 961 CTCAGGACTT ACTGGAGCAA ATCTCAAAGA GCAGTCGGAT TTCTGTCTTG GGTACATTGG 1021 GATTTACCGT TTATTCATAT CTTGGATTAT TTGCCGAACT CCATTAGATC AGGTGATGGG 1081 ACCAATCAGC TGCTATGATG ATAGTTACAA GGAATATGGG CAACCTGATG G // |
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| Primer: pGEX-R | RDB13270_A7Drb.seq |
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>13270_13270_A7Dr_pGEX-R_E10_13_ABI24.ab1 1 CGGGTTAATC GGGAACGCGC GAGGCGGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT 61 TAGTCTTTCT CTGGAACCAG TTTCAGAACT TTTCCAATTG CAATGGTCTT ACCCTCATCT 121 CTTAAGGTGA AACGACCCAT CTGAGGGAAG TCTTTAAAGG TCTCAAGGCA GATGGTTCCT 181 GCTGTCCTTA AGCGAGCAAT GCACACTTGA TCTTGTTTCA CAAAACGGGG TCGGGTCTTA 241 CTTTTTTCTC CTGATTTTTT GTCTACCAAG CAGATTAAGG CTGTTATTTC CACCTCCTCA 301 ATACAGGTAT GAATATGCAG CACCGCATTA TAGCCTGGGC AGATGATGGA TTTGTGCTCT 361 ATAATCACTA TCTGGGCATC AAATGTGCGT CCAGAATGAC AAAGATTATT AGGATCACAA 421 AGTATAAACC CTGGAAGAAT CTCCTCTTCT TCAATTCCTT TCAGTCTGAT TTTGAGGTTT 481 TCACCTGGGG CTACGGTATC AGTCTCTACA TCATCGGAAA GTATTCCAAG AACTTCCACG 541 TTGTGCTTGT TTGGCATCAT CACAAGCTGC TGGCCTTTAC AAATAGATCC TGATTCCAGC 601 TTTCCCAGGA CCACAGTGCC CATATCCTTG TACTTATCCA CAATTGGCAG CCTGATTGGT 661 CCATCAACTG ATCTATTGAA GTTCGGCAAA TTATCCAGAT ATGGAATAAA CGGTAATCCA 721 ATGTACCAAG GACAGAAATC CGACTGCTCT TTGAGATTTG CTCCAGTAAG TCCTGAGCAG 781 GGCATAAAGT GAATGTCCTT TTTGGGATTG AAGCCAACTT TTTTTCAAAA ATGGCACTAG 841 TTTCTCCTTA CATTCTTCAT ATCTCTCATT GCTCCAATTT ACTGTTGGAT CATCCATCTT 901 ATTAATTAGC ACAATTAGGT GTTTTACACC TGCTGTCTTT GCCAACATTG CATGTTCTCT 961 TGTCTGTCCT CCTTTTTTCA AATCCAGTTT CAACTCTCCT TTCCCTGGCT GAGATACCAG 1021 CACAGCCCAA ATCAGCTGGA GAGCACACGA TCATATTTGG GACCAAACTC TGTGCAGGGG 1081 CATCTAGATG TCAAATGCTT CCTTTTCCGA TTCAAAATAG GCCACGGATT CT // |
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| Primer: pGEX-ptac_up-F | RDB13270_A7Drc.seq |
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>13270_13270_A7Dr_pGEX-ptac_up-F_F10_16_ABI24.ab1 1 AAAAGCCCCG CTCATAACGG TTCTGGCAAT ATTCTGAAAT GAGCTGTTGA CAATTAATCA 61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT 121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT 181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA 241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA 301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA 361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT 421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT 481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA 541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA 601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA 661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC 721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC GACCATCCTC CAAAATCGGA 781 TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCCCGGAA TTCATGGAAC TTTCAGAACC 841 TATTGTAGAA AATGGAGAGA CAGAAATGTC TCCAGAAGAA TCATGGGAGC ACAAAGAAGA 901 AATAAGTGAA GCAGAGCCAG GGGGTGGTTC CTTGGGAGAT GGAAAGGCCG CCAGAGGAAA 961 GTGCCCATGA AATGATGGGA GGAGGAAGAG GAAATCCCAA AAACCTAAAG TCTGTGGTTG 1021 CACCGCCCAG GGTGCTCTAG AAAGAGCATG TAAATGTAGT ATCATGGGGC ACGTAGATGC 1081 TGCAAGGTCA ATCAATGGGA GACAAATTAA TGCTAATTTG AACCTGGG // |
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| Primer: M13_-40 | RDB13270_A7Drd.seq |
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>13270_13270_A7Dr_M13(-40)_G10_19_ABI24.ab1 1 CAGGCGCGAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 61 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 121 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 181 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 241 AGCGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG 301 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT 361 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT 421 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC 481 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC 541 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA 601 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA 661 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG 721 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG 781 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG 841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC 901 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC 961 AGTTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGCGCGT GCAGGCAGAC 1021 TGGAGTTGCC ACCGCATCAG CCACGACTGT TTGGCCCGCA GCTGGTGTGC ACGGCGTTTG 1081 GAATGTATCA GCTTCCGGCA TCGACGCTTC AACTTTTTGC CG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. |
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| reference | Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838. |
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl