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pGEX6P-eRF3 (#RDB13270)

Expression vector of human eRF3

Clone info. Expression vector of human eRF3.
Comment Expression was confirmed by depositor with Western blot and CBB staining. cDNA fragment 663..2162 of NM_002094.3 is cloned. Known difference (at least): C to G substitution at 2,003nt (Val, synonym), A to G substitution at 2,081nt (Val, synonym).
Vector backbone pGEX6P (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human eRF3 cDNA
Depositor|Developer Imataka, Hiroaki |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。

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RDB13270 pGEX6P-eRF3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-eRF3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13270).

Reference section:

Machida, K., Mikami, S., Masutani, M., Mishima, K., Kobayashi, T., Imataka, H., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13270_A7Drp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB13270_A7Dra.seq check
>13270_13270_A7Dr_pGEX-F2_D10_10_ABI24.ab1
    1 GTAGTAGTTT GTTTTAAAAA CGTATTGAGC TATCCCACAA ATTGATAAGT ACTTGAAATC
   61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA
  121 TCCTCCAAAA TCGGATCTGG AAGTTCTGTT CCAGGGGCCC CTGGGATCCC CGGAATTCAT
  181 GGAACTTTCA GAACCTATTG TAGAAAATGG AGAGACAGAA ATGTCTCCAG AAGAATCATG
  241 GGAGCACAAA GAAGAAATAA GTGAAGCAGA GCCAGGGGGT GGTTCCTTGG GAGATGGAAG
  301 GCCGCCAGAG GAAAGTGCCC ATGAAATGAT GGAGGAGGAA GAGGAAATCC CAAAACCTAA
  361 GTCTGTGGTT GCACCGCCAG GTGCTCCTAA GAAAGAGCAT GTAAATGTAG TATTCATTGG
  421 GCACGTAGAT GCTGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT
  481 TGACAAAAGG ACGCTTGAAA AGTATGAAAG AGAAGCTAAA GAGAAAAACA GAGAAACTTG
  541 GTACTTGTCT TGGGCCTTAG ACACAAATCA GGAAGAACGA GACAAGGGTA AAACAGTAGA
  601 AGTGGGTCGT GCCTATTTTG AAACCGAAAA GAAGCATTTC ACAATTCTAG ATGCCCCTGG
  661 CCACAAGAGT TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGATT TGGCTGTGCT
  721 GGTAATCTCA GCCAGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG
  781 AGAACATGCA ATGTTGGCAA AGACAGCAGG TGTAAAACAC CTAATTGTGC TAATTAATAA
  841 GATGGATGAT CCAACAGTAA ATTGGAGCAA TGAGAGATAT GAAGAATGTA AGGAGAAACT
  901 AGTGCCATTT TTGAAAAAAG TTGGCTTCAA TCCCAAAAAG GACATTCACT TTATGCCCTG
  961 CTCAGGACTT ACTGGAGCAA ATCTCAAAGA GCAGTCGGAT TTCTGTCTTG GGTACATTGG
 1021 GATTTACCGT TTATTCATAT CTTGGATTAT TTGCCGAACT CCATTAGATC AGGTGATGGG
1081 ACCAATCAGC TGCTATGATG ATAGTTACAA GGAATATGGG CAACCTGATG G
//
Primer: pGEX-R
Sequence file: RDB13270_A7Drb.seq check
>13270_13270_A7Dr_pGEX-R_E10_13_ABI24.ab1
    1 CGGGTTAATC GGGAACGCGC GAGGCGGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT
   61 TAGTCTTTCT CTGGAACCAG TTTCAGAACT TTTCCAATTG CAATGGTCTT ACCCTCATCT
  121 CTTAAGGTGA AACGACCCAT CTGAGGGAAG TCTTTAAAGG TCTCAAGGCA GATGGTTCCT
  181 GCTGTCCTTA AGCGAGCAAT GCACACTTGA TCTTGTTTCA CAAAACGGGG TCGGGTCTTA
  241 CTTTTTTCTC CTGATTTTTT GTCTACCAAG CAGATTAAGG CTGTTATTTC CACCTCCTCA
  301 ATACAGGTAT GAATATGCAG CACCGCATTA TAGCCTGGGC AGATGATGGA TTTGTGCTCT
  361 ATAATCACTA TCTGGGCATC AAATGTGCGT CCAGAATGAC AAAGATTATT AGGATCACAA
  421 AGTATAAACC CTGGAAGAAT CTCCTCTTCT TCAATTCCTT TCAGTCTGAT TTTGAGGTTT
  481 TCACCTGGGG CTACGGTATC AGTCTCTACA TCATCGGAAA GTATTCCAAG AACTTCCACG
  541 TTGTGCTTGT TTGGCATCAT CACAAGCTGC TGGCCTTTAC AAATAGATCC TGATTCCAGC
  601 TTTCCCAGGA CCACAGTGCC CATATCCTTG TACTTATCCA CAATTGGCAG CCTGATTGGT
  661 CCATCAACTG ATCTATTGAA GTTCGGCAAA TTATCCAGAT ATGGAATAAA CGGTAATCCA
  721 ATGTACCAAG GACAGAAATC CGACTGCTCT TTGAGATTTG CTCCAGTAAG TCCTGAGCAG
  781 GGCATAAAGT GAATGTCCTT TTTGGGATTG AAGCCAACTT TTTTTCAAAA ATGGCACTAG
  841 TTTCTCCTTA CATTCTTCAT ATCTCTCATT GCTCCAATTT ACTGTTGGAT CATCCATCTT
  901 ATTAATTAGC ACAATTAGGT GTTTTACACC TGCTGTCTTT GCCAACATTG CATGTTCTCT
  961 TGTCTGTCCT CCTTTTTTCA AATCCAGTTT CAACTCTCCT TTCCCTGGCT GAGATACCAG
 1021 CACAGCCCAA ATCAGCTGGA GAGCACACGA TCATATTTGG GACCAAACTC TGTGCAGGGG
1081 CATCTAGATG TCAAATGCTT CCTTTTCCGA TTCAAAATAG GCCACGGATT CT
//
Primer: pGEX-ptac_up-F
Sequence file: RDB13270_A7Drc.seq check
>13270_13270_A7Dr_pGEX-ptac_up-F_F10_16_ABI24.ab1
    1 AAAAGCCCCG CTCATAACGG TTCTGGCAAT ATTCTGAAAT GAGCTGTTGA CAATTAATCA
   61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT
  121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT
  181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA
  241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA
  301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA
  361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT
  421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT
  481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA
  541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA
  601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA
  661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC
  721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC GACCATCCTC CAAAATCGGA
  781 TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCCCGGAA TTCATGGAAC TTTCAGAACC
  841 TATTGTAGAA AATGGAGAGA CAGAAATGTC TCCAGAAGAA TCATGGGAGC ACAAAGAAGA
  901 AATAAGTGAA GCAGAGCCAG GGGGTGGTTC CTTGGGAGAT GGAAAGGCCG CCAGAGGAAA
  961 GTGCCCATGA AATGATGGGA GGAGGAAGAG GAAATCCCAA AAACCTAAAG TCTGTGGTTG
 1021 CACCGCCCAG GGTGCTCTAG AAAGAGCATG TAAATGTAGT ATCATGGGGC ACGTAGATGC
1081 TGCAAGGTCA ATCAATGGGA GACAAATTAA TGCTAATTTG AACCTGGG
//
Primer: M13_-40
Sequence file: RDB13270_A7Drd.seq check
>13270_13270_A7Dr_M13(-40)_G10_19_ABI24.ab1
    1 CAGGCGCGAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
   61 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
  121 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
  181 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT
  241 AGCGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG
  301 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT
  361 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT
  421 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC
  481 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC
  541 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA
  601 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA
  661 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG
  721 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG
  781 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
  841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC
  901 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC
  961 AGTTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGCGCGT GCAGGCAGAC
 1021 TGGAGTTGCC ACCGCATCAG CCACGACTGT TTGGCCCGCA GCTGGTGTGC ACGGCGTTTG
1081 GAATGTATCA GCTTCCGGCA TCGACGCTTC AACTTTTTGC CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.