Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEX6P-eRF3

Expression vector of human eRF3

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13270_A7Drp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 Sequence file: RDB13270_A7Dra.seq
>13270_13270_A7Dr_pGEX-F2_D10_10_ABI24.ab1     1 GTAGTAGTTT GTTTTAAAAA CGTATTGAGC TATCCCACAA ATTGATAAGT ACTTGAAATC    61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA   121 TCCTCCAAAA TCGGATCTGG AAGTTCTGTT CCAGGGGCCC CTGGGATCCC CGGAATTCAT   181 GGAACTTTCA GAACCTATTG TAGAAAATGG AGAGACAGAA ATGTCTCCAG AAGAATCATG   241 GGAGCACAAA GAAGAAATAA GTGAAGCAGA GCCAGGGGGT GGTTCCTTGG GAGATGGAAG   301 GCCGCCAGAG GAAAGTGCCC ATGAAATGAT GGAGGAGGAA GAGGAAATCC CAAAACCTAA   361 GTCTGTGGTT GCACCGCCAG GTGCTCCTAA GAAAGAGCAT GTAAATGTAG TATTCATTGG   421 GCACGTAGAT GCTGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT   481 TGACAAAAGG ACGCTTGAAA AGTATGAAAG AGAAGCTAAA GAGAAAAACA GAGAAACTTG   541 GTACTTGTCT TGGGCCTTAG ACACAAATCA GGAAGAACGA GACAAGGGTA AAACAGTAGA   601 AGTGGGTCGT GCCTATTTTG AAACCGAAAA GAAGCATTTC ACAATTCTAG ATGCCCCTGG   661 CCACAAGAGT TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGATT TGGCTGTGCT   721 GGTAATCTCA GCCAGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG   781 AGAACATGCA ATGTTGGCAA AGACAGCAGG TGTAAAACAC CTAATTGTGC TAATTAATAA   841 GATGGATGAT CCAACAGTAA ATTGGAGCAA TGAGAGATAT GAAGAATGTA AGGAGAAACT   901 AGTGCCATTT TTGAAAAAAG TTGGCTTCAA TCCCAAAAAG GACATTCACT TTATGCCCTG   961 CTCAGGACTT ACTGGAGCAA ATCTCAAAGA GCAGTCGGAT TTCTGTCTTG GGTACATTGG  1021 GATTTACCGT TTATTCATAT CTTGGATTAT TTGCCGAACT CCATTAGATC AGGTGATGGG 1081 ACCAATCAGC TGCTATGATG ATAGTTACAA GGAATATGGG CAACCTGATG G //
Primer: pGEX-R Sequence file: RDB13270_A7Drb.seq
>13270_13270_A7Dr_pGEX-R_E10_13_ABI24.ab1     1 CGGGTTAATC GGGAACGCGC GAGGCGGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT    61 TAGTCTTTCT CTGGAACCAG TTTCAGAACT TTTCCAATTG CAATGGTCTT ACCCTCATCT   121 CTTAAGGTGA AACGACCCAT CTGAGGGAAG TCTTTAAAGG TCTCAAGGCA GATGGTTCCT   181 GCTGTCCTTA AGCGAGCAAT GCACACTTGA TCTTGTTTCA CAAAACGGGG TCGGGTCTTA   241 CTTTTTTCTC CTGATTTTTT GTCTACCAAG CAGATTAAGG CTGTTATTTC CACCTCCTCA   301 ATACAGGTAT GAATATGCAG CACCGCATTA TAGCCTGGGC AGATGATGGA TTTGTGCTCT   361 ATAATCACTA TCTGGGCATC AAATGTGCGT CCAGAATGAC AAAGATTATT AGGATCACAA   421 AGTATAAACC CTGGAAGAAT CTCCTCTTCT TCAATTCCTT TCAGTCTGAT TTTGAGGTTT   481 TCACCTGGGG CTACGGTATC AGTCTCTACA TCATCGGAAA GTATTCCAAG AACTTCCACG   541 TTGTGCTTGT TTGGCATCAT CACAAGCTGC TGGCCTTTAC AAATAGATCC TGATTCCAGC   601 TTTCCCAGGA CCACAGTGCC CATATCCTTG TACTTATCCA CAATTGGCAG CCTGATTGGT   661 CCATCAACTG ATCTATTGAA GTTCGGCAAA TTATCCAGAT ATGGAATAAA CGGTAATCCA   721 ATGTACCAAG GACAGAAATC CGACTGCTCT TTGAGATTTG CTCCAGTAAG TCCTGAGCAG   781 GGCATAAAGT GAATGTCCTT TTTGGGATTG AAGCCAACTT TTTTTCAAAA ATGGCACTAG   841 TTTCTCCTTA CATTCTTCAT ATCTCTCATT GCTCCAATTT ACTGTTGGAT CATCCATCTT   901 ATTAATTAGC ACAATTAGGT GTTTTACACC TGCTGTCTTT GCCAACATTG CATGTTCTCT   961 TGTCTGTCCT CCTTTTTTCA AATCCAGTTT CAACTCTCCT TTCCCTGGCT GAGATACCAG  1021 CACAGCCCAA ATCAGCTGGA GAGCACACGA TCATATTTGG GACCAAACTC TGTGCAGGGG 1081 CATCTAGATG TCAAATGCTT CCTTTTCCGA TTCAAAATAG GCCACGGATT CT //
Primer: pGEX-ptac_up-F Sequence file: RDB13270_A7Drc.seq
>13270_13270_A7Dr_pGEX-ptac_up-F_F10_16_ABI24.ab1     1 AAAAGCCCCG CTCATAACGG TTCTGGCAAT ATTCTGAAAT GAGCTGTTGA CAATTAATCA    61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT   121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT   181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA   241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA   301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA   361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT   421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT   481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA   541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA   601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA   661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC   721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC GACCATCCTC CAAAATCGGA   781 TCTGGAAGTT CTGTTCCAGG GGCCCCTGGG ATCCCCGGAA TTCATGGAAC TTTCAGAACC   841 TATTGTAGAA AATGGAGAGA CAGAAATGTC TCCAGAAGAA TCATGGGAGC ACAAAGAAGA   901 AATAAGTGAA GCAGAGCCAG GGGGTGGTTC CTTGGGAGAT GGAAAGGCCG CCAGAGGAAA   961 GTGCCCATGA AATGATGGGA GGAGGAAGAG GAAATCCCAA AAACCTAAAG TCTGTGGTTG  1021 CACCGCCCAG GGTGCTCTAG AAAGAGCATG TAAATGTAGT ATCATGGGGC ACGTAGATGC 1081 TGCAAGGTCA ATCAATGGGA GACAAATTAA TGCTAATTTG AACCTGGG //
Primer: M13_-40 Sequence file: RDB13270_A7Drd.seq
>13270_13270_A7Dr_M13(-40)_G10_19_ABI24.ab1     1 CAGGCGCGAT CGTATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA    61 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA   121 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT   181 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT   241 AGCGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG   301 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT   361 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT   421 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC   481 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC   541 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA   601 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA   661 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG   721 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG   781 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG   841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC   901 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC   961 AGTTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGCGCGT GCAGGCAGAC  1021 TGGAGTTGCC ACCGCATCAG CCACGACTGT TTGGCCCGCA GCTGGTGTGC ACGGCGTTTG 1081 GAATGTATCA GCTTCCGGCA TCGACGCTTC AACTTTTTGC CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl