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pUC-T7-EMCV-His-eEF1Bgamma

EMCV IRES-dependent expression vector of human eEF1Bgamma

Catalog number RDB13267
Resource name pUC-T7-EMCV-His-eEF1Bgamma
Clone info. EMCV IRES-dependent expression vector of human eEF1Bgamma.
Expression was confirmed by depositor with Western blot.
Vector backbone pUC-T7-EMCV-His (Plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Human eEF1Bgamma cDNA
Depositor Imataka, Hiroaki |
 
Other clones in our bank human EEF1G (NCBI Gene 1937) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13267 pUC-T7-EMCV-His-eEF1Bgamma Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

2019.07.20

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