Resource data sheet
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pUC-T7-EMCV-His-eEF1A (#RDB13266)

EMCV IRES-dependent expression vector of human eEF1A

Clone info. EMCV IRES-dependent expression vector of human eEF1A.
Comment Expression was confirmed by depositor with Western blot.
Vector backbone pUC-T7-EMCV-His (Plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Human eEF1A cDNA
Depositor|Developer Imataka, Hiroaki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13266 pUC-T7-EMCV-His-eEF1A Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUC-T7-EMCV-His-eEF1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13266).

Reference section:

Machida, K., Mikami, S., Masutani, M., Mishima, K., Kobayashi, T., Imataka, H., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322. [link to RRC of NBRP]
reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.