RDB13243 - PB-TAC-ERN

PB-TAC-ERN (#RDB13243)

All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs and mCherry reporter.

Drawn by SnapGene® software

Sequence (full)	RDB13243hts01.seq
Publication	Kim, S.I., Methods Mol. Biol. 1357: 111-131 (2016). [link to RRC of NBRP]
Test sheet	RDB13243C0p1-1.pdf

Assembled from experimentally sequenced data.

Alternative name	KW111
Clone info.	All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs and mCherry reporter.
Comment	Expression was confirmed by the depositor with human and mouse ES/iPS cells, differentiated cells, and common cell lines (HeLa, HEK293T). Relation: KW110 (without ires-linked reporter: RDB13242); KW200 (with ires-linked GFP reporter: RDB13244). Remarks: Alternative All-in-One piggyBac vectors available (selection, reporter): KW110 (RDB13242), KW200 (RDB13244), KW542 (RDB13245), KW441 (RDB13247), KW543 (RDB13246).
Vector backbone	Plasmid
Size of vector backbone	3.0kb
Selectable markers	Amp^r
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	CiRA, Kyoto Univ. \| Woltjen, Knut \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Approval form by depositor [Word]
This RESOURCE contains a piggyBac® transposon. Limited Use License Agreement (LULA) [Word] is also required.
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution For provision of this material, prior permission by the Depositor (Kyoto University cira-keiyaku@cira.kyoto-u.ac.jp) is required. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Kim et al., Methods Mol. Biol. 1357: 111-131 (2016)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.

Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Use DB3.1(Invitrogen) or equivalent to amplify this clone.
Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Approval form by depositor [Word] This RESOURCE contains a piggyBac® transposon. Limited Use License Agreement (LULA) [Word] is also required. Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	For provision of this material, prior permission by the Depositor (Kyoto University cira-keiyaku@cira.kyoto-u.ac.jp) is required. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Kim et al., Methods Mol. Biol. 1357: 111-131 (2016)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
Remarks	An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Use DB3.1(Invitrogen) or equivalent to amplify this clone. Please obtain written approval of the depositor.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。寄託者による提供承諾書 [Word] piggyBac®トランスポゾンを内包するバイオリソースです。Limited Use License Agreement (LULA) [Word]の提出をお願いします。手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、事前に寄託者 (連絡先：京都大学 cira-keiyaku@cira.kyoto-u.ac.jp) から提供承諾を得る。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Kim et al., Methods Mol. Biol. 1357: 111-131 (2016))。学術機関の学術研究に限る。
備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13243	PB-TAC-ERN	DNA solution

How to cite this biological resource

Materials & Methods section:

The PB-TAC-ERN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13243).

Reference section:

Kim, S.I., Oceguera-Yanez, F., Sakurai, C., Nakagawa, M., Yamanaka, S., Woltjen, K., Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol. Biol. 1357: 111-131 (2016). PMID 26025620. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Kim, S.I., Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol. Biol. 1357: 111-131 (2016). PMID 26025620. [link to RRC of NBRP]
reference	Tanaka, A., Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro. PLoS One 8 (4): e61540 (2013). PMID 23626698.

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