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PB-TA-ERN (#RDB13242)

All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs.


Drawn by SnapGene® software
Sequence (full) RDB13242hts01.seq
Publication Kim, S.I., Methods Mol. Biol. 1357: 111-131 (2016). [link to RRC of NBRP]
Test sheet RDB13242C0p1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name KW110
Clone info. All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs.
Comment Expression was confirmed by the depositor with human and mouse ES/iPS cells, differentiated cells, and common cell lines (HeLa, HEK293T).
Relation: KW111 (with ires-linked mCherry reporter: RDB13243); KW200 (with ires-linked GFP reporter: RDB13244).
Remarks: Alternative All-in-One piggyBac vectors available (selection, reporter): KW111 (RDB13243), KW200 (RDB13244), KW542 (RDB13245), KW441 (RDB13247), KW543 (RDB13246).
Vector backbone PB-TA-ERN (Plasmid)
Size of vector backbone 3.0kb
Selectable markers Amp^r
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer CiRA, Kyoto Univ. | Woltjen, Knut |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
This RESOURCE contains a piggyBac® transposon. Limited Use License Agreement (LULA) [Word] is also required.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution For provision of this material, prior permission by the Depositor (Kyoto University cira-keiyaku@cira.kyoto-u.ac.jp) is required. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Kim et al., Methods Mol. Biol. 1357: 111-131 (2016)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
Remarks Use DB3.1(Invitrogen) or equivalent to amplify this clone.
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
piggyBac®トランスポゾンを内包するバイオリソースです。Limited Use License Agreement (LULA) [Word]の提出をお願いします。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、事前に寄託者 (連絡先：京都大学 cira-keiyaku@cira.kyoto-u.ac.jp) から提供承諾を得る。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Kim et al., Methods Mol. Biol. 1357: 111-131 (2016))。学術機関の学術研究に限る。
備考 このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13242 PB-TA-ERN DNA solution

How to cite this biological resource

Materials & Methods section:

The PB-TA-ERN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13242).

Reference section:

Kim, S.I., Oceguera-Yanez, F., Sakurai, C., Nakagawa, M., Yamanaka, S., Woltjen, K., Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol. Biol. 1357: 111-131 (2016). PMID 26025620. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kim, S.I., Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol. Biol. 1357: 111-131 (2016). PMID 26025620. [link to RRC of NBRP]