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p3xFLAG-CMV-14_mLPH (#RDB13240)

Expression plasmid of mouse LPH

Clone info. Expression plasmid of mouse LPH. LPH was cloned from genomic DNA of mouse small intestine.
Comment Expression was confirmed by the depositor with anti-FLAG M2 antibody.
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse LPH cDNA
Depositor|Developer Murofushi, Kimiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 441 (4), 838-843, 2013)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 441 (4), 838-843, 2013)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13240 p3xFLAG-CMV-14_mLPH DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV-14_mLPH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13240).

Reference section:

Akiyama, H., Kobayashi, S., Hirabayashi, Y., Murakami-Murofushi, K., Cholesterol glucosylation is catalyzed by transglucosylation reaction of beta-glucosidase 1. Biochem. Biophys. Res. Commun. 441 (4): 838-843 (2013). PMID 24211208. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13240_B2Bhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB13240_B2Bha.seq check
>13240_13240_B2Bh_1_CMV_Forward_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
   61 GCTCGTTTAG TGAACCGTCA GAATTAAGCT TGCGGCCGCG AATTCGCCAC CATGGAGCTG
  121 CCTTGGACAG CACTCTTCCT CAGTACCTTC CTCCTGGGTC TTTCATGCCA GGGGAATGAC
  181 TGGGAATCTG ACAGAAATTT TATTTCAGCT GCTGGTCCTC TAACAAATGA CTTAGTGCAC
  241 AATCTGAACC ATCCGCTGGG AAAGCAGGGC TCTGATTTGG TAGCTGGTGA TACTGACATC
  301 TATGTCTGCC AGCAGCCACT GCCCTCCTTC TTGCCTCAGT ACTTCAGCAG TCTTCGGGCC
  361 AGCCAGGTTA CCCATTACAA AGTACTTCTG TCATGGGCAC AACTTCTCCC AAAAGGAAGC
  421 TCCAAGAATC CAGACCAGGA AGCAGTGCAG TGCTACCGAC AACTCCTCCA GTCCCTGAAG
  481 GATGCCCAGC TTCAGCCCAT GGTGGTCCTG TTCCACCAGA TGCCACCTAC NAGCACCATC
  541 NNGAGAGACG GGGGCTTTGC TGACCTCTTT GCTGANTATG CCACNNNGGC TTTTCNGNNC
  601 TTTGGGGNNC TTGNNGAGAT CNGGNTTCNC CNNNNNNGNN TNGNNNAAAA GTGATNNTGG
661 NNNNNNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGHpA,3XFLAG,insert 3'
Sequence file: RDB13240_B2Bhb.seq check
>13240_13240_B2Bh_1_hGHpolyA-R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNATATTA TAGAAGGACA CCTAGTCAGA CAAAATGATG CAACTTAATT
   61 TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG AGAGGCACTG
  121 GGGAGGGGTC ACAGGGATGC CACCCGGGAT CACTACTTGT CATCGTCATC CTTGTAGTCG
  181 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCCTCTAGAG
  241 AAGGAAGAAA TTTGACTCAG ACCGTGGTGG CCTGGCTGTG TTGTCCCTTG CTTGGAGCGC
  301 TTGCAGTATT TGTATAACAG AAATGCCACG CTGCAAACTC CAAGCAGCAC AAGAGCGAAG
  361 AGGACATACA GAGCCGTCTG TGCTTCTGCC GTGCCCAGCA TCAGCCCCAG GAAGGGCACC
  421 TCTGACTTCA CAGGGCTGGC AGTGGGCCCA GCATCTTCTG GTTGCTGGAG ACAAGGATGA
  481 GGTCCTTGTG CAGGGTCAGG AAAGCCATTG CAGCGGACTA TGGAGGCGTA GACCTTGGCT
  541 GACGCCTTGG GGATCCTTGN CAAAGAAGGN NCANAGCGGT TNNCAAAGTG CACNNCGAAC
  601 CTCTCTGCNN NNNCTGTGGC CCATTCNAAG TTNNNCNNTG NNGCTCNNNN NCGTGTNNCC
661 NCNANNGNNN NCCNTNNTCT CNNNNAGCTT TNANGNNNNN ATNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB13240_B2Bhc.seq check
>13240_13240_B2Bh_1_SV40pro_ori_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT CTGAGCTATT CNNGAAGTAG TGAGGAGGCT TTTTTGGAGG
   61 AATTGATCAG CTTGGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC
  121 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT
  181 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC
  241 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG
  301 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG
  361 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT
  421 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC
  481 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG
  541 CACGTACTCG GATGGAANNC CGGTCTTGTC GATNNGGATG ATCTGGANNA NNANNNNNNA
  601 NGGGCTCGCG CCNNNNCGAN NTGGTNCNNC CNNGCTNNNN GGCGCGNNNG CCCNNANNGN
661 CNNNGNANNN TCNNNNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Akiyama, H., Cholesterol glucosylation is catalyzed by transglucosylation reaction of beta-glucosidase 1. Biochem. Biophys. Res. Commun. 441 (4): 838-843 (2013). PMID 24211208. [link to RRC of NBRP]
reference Kobayashi, T., The glycosylceramidase in the murine intestine. Purification and substrate specificity. J. Biol. Chem. 256 (15): 7768-7773 (1981). PMID 6790523. [link to RRC of NBRP]