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p3xFLAG-CMV-14_mLPH

Expression plasmid of mouse LPH

Catalog number RDB13240
Resource name p3xFLAG-CMV-14_mLPH
Clone info. Expression plasmid of mouse LPH. LPH was cloned from genomic DNA of mouse small intestine.
Comment Expression was confirmed by the depositor with anti-FLAG M2 antibody.
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse LPH cDNA
Depositor|Developer Murofushi, Kimiko |
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External Database
Mouse LPH

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 441 (4), 838-843, 2013)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 441 (4), 838-843, 2013)。
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          RDB13240 p3xFLAG-CMV-14_mLPH DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The p3xFLAG-CMV-14_mLPH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13240).

          Reference section:

          Akiyama, H., Kobayashi, S., Hirabayashi, Y., Murakami-Murofushi, K., Cholesterol glucosylation is catalyzed by transglucosylation reaction of beta-glucosidase 1. Biochem. Biophys. Res. Commun. 441 (4): 838-843 (2013). PMID 24211208.

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13240_B2Bhp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB13240_B2Bha.seq check
          >13240_13240_B2Bh_1_CMV_Forward_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
             61 GCTCGTTTAG TGAACCGTCA GAATTAAGCT TGCGGCCGCG AATTCGCCAC CATGGAGCTG
            121 CCTTGGACAG CACTCTTCCT CAGTACCTTC CTCCTGGGTC TTTCATGCCA GGGGAATGAC
            181 TGGGAATCTG ACAGAAATTT TATTTCAGCT GCTGGTCCTC TAACAAATGA CTTAGTGCAC
            241 AATCTGAACC ATCCGCTGGG AAAGCAGGGC TCTGATTTGG TAGCTGGTGA TACTGACATC
            301 TATGTCTGCC AGCAGCCACT GCCCTCCTTC TTGCCTCAGT ACTTCAGCAG TCTTCGGGCC
            361 AGCCAGGTTA CCCATTACAA AGTACTTCTG TCATGGGCAC AACTTCTCCC AAAAGGAAGC
            421 TCCAAGAATC CAGACCAGGA AGCAGTGCAG TGCTACCGAC AACTCCTCCA GTCCCTGAAG
            481 GATGCCCAGC TTCAGCCCAT GGTGGTCCTG TTCCACCAGA TGCCACCTAC NAGCACCATC
            541 NNGAGAGACG GGGGCTTTGC TGACCTCTTT GCTGANTATG CCACNNNGGC TTTTCNGNNC
            601 TTTGGGGNNC TTGNNGAGAT CNGGNTTCNC CNNNNNNGNN TNGNNNAAAA GTGATNNTGG
          661 NNNNNNNNNN
          //
          Primer: hGHpolyA-R (Pr0350)
          Region: hGHpA,3XFLAG,insert 3'
          Sequence file: RDB13240_B2Bhb.seq check
          >13240_13240_B2Bh_1_hGHpolyA-R_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNATATTA TAGAAGGACA CCTAGTCAGA CAAAATGATG CAACTTAATT
             61 TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG AGAGGCACTG
            121 GGGAGGGGTC ACAGGGATGC CACCCGGGAT CACTACTTGT CATCGTCATC CTTGTAGTCG
            181 ATGTCATGAT CTTTATAATC ACCGTCATGG TCTTTGTAGT CAGCCCGGGA TCCTCTAGAG
            241 AAGGAAGAAA TTTGACTCAG ACCGTGGTGG CCTGGCTGTG TTGTCCCTTG CTTGGAGCGC
            301 TTGCAGTATT TGTATAACAG AAATGCCACG CTGCAAACTC CAAGCAGCAC AAGAGCGAAG
            361 AGGACATACA GAGCCGTCTG TGCTTCTGCC GTGCCCAGCA TCAGCCCCAG GAAGGGCACC
            421 TCTGACTTCA CAGGGCTGGC AGTGGGCCCA GCATCTTCTG GTTGCTGGAG ACAAGGATGA
            481 GGTCCTTGTG CAGGGTCAGG AAAGCCATTG CAGCGGACTA TGGAGGCGTA GACCTTGGCT
            541 GACGCCTTGG GGATCCTTGN CAAAGAAGGN NCANAGCGGT TNNCAAAGTG CACNNCGAAC
            601 CTCTCTGCNN NNNCTGTGGC CCATTCNAAG TTNNNCNNTG NNGCTCNNNN NCGTGTNNCC
          661 NCNANNGNNN NCCNTNNTCT CNNNNAGCTT TNANGNNNNN ATNNNNNNNN NN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB13240_B2Bhc.seq check
          >13240_13240_B2Bh_1_SV40pro_ori_F_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT CTGAGCTATT CNNGAAGTAG TGAGGAGGCT TTTTTGGAGG
             61 AATTGATCAG CTTGGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC
            121 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT
            181 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC
            241 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG
            301 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG
            361 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT
            421 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC
            481 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG
            541 CACGTACTCG GATGGAANNC CGGTCTTGTC GATNNGGATG ATCTGGANNA NNANNNNNNA
            601 NGGGCTCGCG CCNNNNCGAN NTGGTNCNNC CNNGCTNNNN GGCGCGNNNG CCCNNANNGN
          661 CNNNGNANNN TCNNNNNGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akiyama, H., Cholesterol glucosylation is catalyzed by transglucosylation reaction of beta-glucosidase 1. Biochem. Biophys. Res. Commun. 441 (4): 838-843 (2013). PMID 24211208.
          reference Kobayashi, T., The glycosylceramidase in the murine intestine. Purification and substrate specificity. J. Biol. Chem. 256 (15): 7768-7773 (1981). PMID 6790523.

          2023.05.01

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