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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmGR 3 (#RDB13197)

Plasmid clone of rat mGluR3 cDNA

Clone info. Plasmid clone of rat mGluR3 cDNA. cDNA fragment 81 to 3,300nt of NM_001105712.1 (CDS, 224-2,863) is cloned. See Fig.1b of Tanabe, Y., Neuron 8 (1) 169-179 (1992).
Comment Known difference: Deletion of one adenine base at 3,180nt within 3' UTR of NM_001105712.1.
Vector backbone pBluescriptII KS(+) (Plasmid)
Gene/insert name Rat mGluR3 cDNA
Reference of insert sequence M92076.1 (DDBJ accession number)
Depositor|Developer Nakanishi, Shigetada |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 8: 169-179, 1992) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 8: 169-179, 1992)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

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RDB13197 pmGR 3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmGR 3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13197).

Reference section:

Tanabe, Y., Masu, M., Ishii, T., Shigemoto, R., Nakanishi, S., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13197_A5Cip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB13197_A5Cia.seq check
>13197_13197_A5Ci_T7_20mer_G03_21.ab1
    1 TAACCGCGGT GCGGCGCTCT AGACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGGTGACGG
   61 GCTCTTTCAG CCCAATCCTC CCTTATCTGA AGGACAGGCA AAAGATTCCA CTTGGAAATG
  121 AGAGAAGACT AGAAGGAAAC ATTGGCTTCA CCAAGTGATA TCTCCAAGAG ATATAGAAAC
  181 AGGATTCATG AAGATGTTGA CAAGACTACA AATTCTTATG TTAGCTTTGT TTTCAAAGGG
  241 ATTTTTACTC TCTTTAGGAG ATCACAACTT TATGAGGAGG GAAATTAAAA TAGAAGGAGA
  301 CCTTGTTTTA GGAGGCTTAT TTCCTATTAA TGAAAAAGGC ACTGGAACTG AAGAGTGTGG
  361 GCGAATCAAT GAAGACCGAG GTATCCAACG CCTGGAGGCC ATGTTGTTTG CCATCGACGA
  421 GATTAACAAA GACAATTACT TGCTCCCAGG AGTGAAGCTG GGTGTTCATA TTTTGGATAC
  481 ATGTTCAAGG GATACCTATG CATTAGAGCA ATCACTGGAG TTTGTCAGGG CATCTTTGAC
  541 TAAAGTGGAT GAAGCCGAGT ATATGTGTCC TGATGGATCA TATGCTATTC AAGAAAACAT
  601 CCCACTGCTC ATTGCAGGAG TCATTGGTGG TTCATACAGC AGTGTTTCCA TACAGGTGGC
  661 AAACCTGCTG AGGCTCTTCC AGATCCCTCA GATAAGCTAC GCCTCCACCA GCGCCAAACT
  721 CAGTGACAAG TCGCGCTATG ATTACTTTGC CAGGACCGTG CCCCCTGACT TCTACCAGGC
  781 CAAAGCCATG GCCGAGATCT TGCGCTTCTT CAACTGGACC TATGTGTCCA CCGTTGCCTC
  841 TGAGGGTGAC TATGGGGGAG ACAGGGATTG AGGCCTTCGA GCAGGAAGCA CGGCTGCGTA
  901 ACATCTGCAT CGCCACTGCT GAAAAGGTGG GCCGCTCCAA CATCCGCAAG TCCTACGACA
  961 GCGTGATACG TGAGCTCCTG CAGAAACCCA AATGCGCGGG TCGTGGTCCC TGTTTCATGC
 1021 GCAGTGACGA CTCACGAGAG CTGATCGCGG GCAGCCGATC GCGTGAATGC CCTTCCATTC
1081 ACTTGGTTAC CAAGCGACCG CCTGGGGGTG CTCAAGGGAA GAGAGCCATT AG
//
Primer: Reverse2
Sequence file: RDB13197_A5Cib.seq check
>RDB13197_A5Ci_1_reverse2_E08_14_ABI24.ab1
    1 GATGGAAAAG GAATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
   61 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCGGCACGA
  121 GGTCGACGCG GCCGCTTTTT TTTTTTTTTT TTTTTTTTTT TACACGTAAT GTAACTTTAA
  181 TATACAACGT AAAAACTCAC CCTAAATTAG TTACAACAAG AACCTACAAA AGACTTCATA
  241 GGAAAAAAGG TTACCTAATA TTGCCACTGT ATTTTTTTTG TTTTGTCTTT TTTGTTTTTC
  301 GTTTTAGTAG ACTGACCCTG TCAGACCCTG TCACCAATGC TCAGCTCTGT AAACAATGGT
  361 TATTTCCATG TCCTGGGGAA GGGGCTGTTA ATTAGGGCAC TAAAATCAGC CTAGAAAGTT
  421 GTAGCACATC ACTACATACC ATACAGTCCT CAAAAATAAT TTATTGTACT GTTTCTAAAA
  481 AAAGAAAAAA AAAGATACTA GGGTTGTTGT TTTGTTCTGT TTCCATGTTC TGGAGCAGCA
  541 TGTGAGCACT TTGTCTAACA GCCTAAAAAC ATGAGAACTG AACTGCGTAT CACAATCACA
  601 GAGATGAGGT GGTGGAGTCC AGGACTTCCC TTCCGTTGCA CACTGTTGGG ACATATGTGC
  661 TTGCAGAGGA CTGAGAATAG GTGGTTGCAG TTCCGCTGAC GCTGAACCTG TTGAGGTGAA
  721 GTCTGTGTGT GACCACATTC TTCTGGGGTT GGAACAGGAC GATGTGCACC TTGGGGGCAA
  781 ACAAACAGCC CAAGACCACG AAACCGCTCA GGCTAACGGA GATGCACATT GTTGTCGTCT
  841 GCACTCTGTA GTCACTTGAT GTCACATAAA ATATAGGGAG GAATGCCAAC CAGATGATGC
  901 AGGTGGTGTA CATGGTGAAG CCTATAAACT TGGCTTCATT GAAGTTTTCA GGACACTTCC
  961 TCGTTTTGAA GGCATACACA GTGCATAGGA TCACCAGACA CGTCATAGGT CAGAAGAAGA
 1021 TCAACATGCT GGAATCTTTG ACATTGCATT TTCAAGATGA CTGTTTCCCG CTTCTTCTGG
 1081 GAAGAGTATC CTTCTAGTTA CTTGAGTCTC AAGATAGCTA ACGGACCATC CAATTGGCAC
1141 CAGGTATTCA ACCAAGGCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tanabe, Y., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649. [link to RRC of NBRP]
user_report Onishi, T., Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. J. Physiol. 597 (13): 3441-3455 (2019). PMID 31087329. [link to RRC of NBRP]