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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmGR 3

Plasmid clone of rat mGluR3 cDNA

Catalog number RDB13197
Resource name pmGR 3
Clone info. Plasmid clone of rat mGluR3 cDNA. cDNA fragment 81 to 3,300nt of NM_001105712.1 (CDS, 224-2,863) is cloned. See Fig.1b of Tanabe, Y., Neuron 8 (1) 169-179 (1992).
Comment Known difference: Deletion of one adenine base at 3,180nt within 3' UTR of NM_001105712.1.
Vector backbone pBluescriptII KS(+) (Plasmid)
Gene/insert name Rat mGluR3 cDNA
Reference of insert sequence M92076.1 (DDBJ accession number)
Depositor|Developer Nakanishi, Shigetada |
Other clones in our bank
External Database
Rat mGluR3
          Reference sequence
            NM_001105712.1
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 8: 169-179, 1992) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 8: 169-179, 1992)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13197 pmGR 3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pmGR 3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13197).

          Reference section:

          Tanabe, Y., Masu, M., Ishii, T., Shigemoto, R., Nakanishi, S., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13197_A5Cip1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB13197_A5Cia.seq check
          >13197_13197_A5Ci_T7_20mer_G03_21.ab1
              1 TAACCGCGGT GCGGCGCTCT AGACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGGTGACGG
             61 GCTCTTTCAG CCCAATCCTC CCTTATCTGA AGGACAGGCA AAAGATTCCA CTTGGAAATG
            121 AGAGAAGACT AGAAGGAAAC ATTGGCTTCA CCAAGTGATA TCTCCAAGAG ATATAGAAAC
            181 AGGATTCATG AAGATGTTGA CAAGACTACA AATTCTTATG TTAGCTTTGT TTTCAAAGGG
            241 ATTTTTACTC TCTTTAGGAG ATCACAACTT TATGAGGAGG GAAATTAAAA TAGAAGGAGA
            301 CCTTGTTTTA GGAGGCTTAT TTCCTATTAA TGAAAAAGGC ACTGGAACTG AAGAGTGTGG
            361 GCGAATCAAT GAAGACCGAG GTATCCAACG CCTGGAGGCC ATGTTGTTTG CCATCGACGA
            421 GATTAACAAA GACAATTACT TGCTCCCAGG AGTGAAGCTG GGTGTTCATA TTTTGGATAC
            481 ATGTTCAAGG GATACCTATG CATTAGAGCA ATCACTGGAG TTTGTCAGGG CATCTTTGAC
            541 TAAAGTGGAT GAAGCCGAGT ATATGTGTCC TGATGGATCA TATGCTATTC AAGAAAACAT
            601 CCCACTGCTC ATTGCAGGAG TCATTGGTGG TTCATACAGC AGTGTTTCCA TACAGGTGGC
            661 AAACCTGCTG AGGCTCTTCC AGATCCCTCA GATAAGCTAC GCCTCCACCA GCGCCAAACT
            721 CAGTGACAAG TCGCGCTATG ATTACTTTGC CAGGACCGTG CCCCCTGACT TCTACCAGGC
            781 CAAAGCCATG GCCGAGATCT TGCGCTTCTT CAACTGGACC TATGTGTCCA CCGTTGCCTC
            841 TGAGGGTGAC TATGGGGGAG ACAGGGATTG AGGCCTTCGA GCAGGAAGCA CGGCTGCGTA
            901 ACATCTGCAT CGCCACTGCT GAAAAGGTGG GCCGCTCCAA CATCCGCAAG TCCTACGACA
            961 GCGTGATACG TGAGCTCCTG CAGAAACCCA AATGCGCGGG TCGTGGTCCC TGTTTCATGC
           1021 GCAGTGACGA CTCACGAGAG CTGATCGCGG GCAGCCGATC GCGTGAATGC CCTTCCATTC
          1081 ACTTGGTTAC CAAGCGACCG CCTGGGGGTG CTCAAGGGAA GAGAGCCATT AG
          //
          Primer: Reverse2
          Sequence file: RDB13197_A5Cib.seq check
          >RDB13197_A5Ci_1_reverse2_E08_14_ABI24.ab1
              1 GATGGAAAAG GAATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
             61 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCGGCACGA
            121 GGTCGACGCG GCCGCTTTTT TTTTTTTTTT TTTTTTTTTT TACACGTAAT GTAACTTTAA
            181 TATACAACGT AAAAACTCAC CCTAAATTAG TTACAACAAG AACCTACAAA AGACTTCATA
            241 GGAAAAAAGG TTACCTAATA TTGCCACTGT ATTTTTTTTG TTTTGTCTTT TTTGTTTTTC
            301 GTTTTAGTAG ACTGACCCTG TCAGACCCTG TCACCAATGC TCAGCTCTGT AAACAATGGT
            361 TATTTCCATG TCCTGGGGAA GGGGCTGTTA ATTAGGGCAC TAAAATCAGC CTAGAAAGTT
            421 GTAGCACATC ACTACATACC ATACAGTCCT CAAAAATAAT TTATTGTACT GTTTCTAAAA
            481 AAAGAAAAAA AAAGATACTA GGGTTGTTGT TTTGTTCTGT TTCCATGTTC TGGAGCAGCA
            541 TGTGAGCACT TTGTCTAACA GCCTAAAAAC ATGAGAACTG AACTGCGTAT CACAATCACA
            601 GAGATGAGGT GGTGGAGTCC AGGACTTCCC TTCCGTTGCA CACTGTTGGG ACATATGTGC
            661 TTGCAGAGGA CTGAGAATAG GTGGTTGCAG TTCCGCTGAC GCTGAACCTG TTGAGGTGAA
            721 GTCTGTGTGT GACCACATTC TTCTGGGGTT GGAACAGGAC GATGTGCACC TTGGGGGCAA
            781 ACAAACAGCC CAAGACCACG AAACCGCTCA GGCTAACGGA GATGCACATT GTTGTCGTCT
            841 GCACTCTGTA GTCACTTGAT GTCACATAAA ATATAGGGAG GAATGCCAAC CAGATGATGC
            901 AGGTGGTGTA CATGGTGAAG CCTATAAACT TGGCTTCATT GAAGTTTTCA GGACACTTCC
            961 TCGTTTTGAA GGCATACACA GTGCATAGGA TCACCAGACA CGTCATAGGT CAGAAGAAGA
           1021 TCAACATGCT GGAATCTTTG ACATTGCATT TTCAAGATGA CTGTTTCCCG CTTCTTCTGG
           1081 GAAGAGTATC CTTCTAGTTA CTTGAGTCTC AAGATAGCTA ACGGACCATC CAATTGGCAC
          1141 CAGGTATTCA ACCAAGGCA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tanabe, Y., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649.
          user_report Onishi, T., Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. J. Physiol. 597 (13): 3441-3455 (2019). PMID 31087329.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl