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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmGR 2 (#RDB13196)

Plasmic clone of rat mGluR2 cDNA

Clone info. Plasmid clone of rat mGluR2 cDNA. cDNA fragment 8 to 3,304nt of NM_001105711.1 (CDS, 199-2.817) is cloned. See Fig.1a of Tanabe, Y., Neuron 8 (1), 169-179 (1992).
Comment Known difference: C to T substitution at 73nt in 5' UTR of NM_001105711.1.
Vector backbone pBluescriptII KS(+) (Plasmid)
Gene/insert name Rat mGluR2 cDNA
Reference of insert sequence M92075.1 (DDBJ accession number)
Depositor|Developer Nakanishi, Shigetada |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 8: 169-179, 1992) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 8: 169-179, 1992)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

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RDB13196 pmGR 2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmGR 2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13196).

Reference section:

Tanabe, Y., Masu, M., Ishii, T., Shigemoto, R., Nakanishi, S., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13196_A5Cip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB13196_A5Cia.seq check
>RDB13196_A5Ci_1_reverse2_H11_08_ABI08.ab1
    1 CGGTTTACCG GGATTACGGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
   61 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCGGCACG
  121 AGGCGAGAGA GCCAGCAAGC GGGAACCGGA GCCCCGCGCC AGAGCTGGGC GCGCCAGGAG
  181 CTGACCCTCT CCTCATCTCT CTTCCTGTCT GTCCTTTCCT GGCCCCTGTT TCCTCCTCTC
  241 TTTGCCTTTG CTGCTTCTGT CCTCACCCCT AGAAGACCCT GGTCTGCTGG ACCTAGCCAT
  301 CCCTTTTGGG GCCATGGAAT CACTGCTTGG GTTTCTGGCA CTGCTGCTGC TGTGGGGTGC
  361 CGTGGCCGAG GGCCCGGCCA AGAAGGTGCT GACCCTGGAG GGGGACCTGG TGCTGGGTGG
  421 GCTGTTCCCA GTGCACCAGA AGGGTGGCCC AGCCGAGGAG TGTGGACCTG TTAATGAGCA
  481 CCGAGGCATA CAGCGCCTAG AGGCTATGCT TTTTGCACTG GACCGCATCA ACCGCGACCC
  541 CCACCTGCTG CCTGGTGTGC GCTTGGGTGC GCACATCCTC GACAGCTGCT CCAAGGATAC
  601 ACACGCCCTG GAGCAGGCGC TGGACTTTGT GCGTGCCTCA CTCAGTCGTG GCGCTGACGG
  661 CTCACGCCAC ATCTGTCCTG ATGGCTCCTA TGCCACCCAC AGTGATGCTC CTACAGCTGT
  721 CACCGGTGTC ATTGGTGGCT CCTACAGTGA TGTCTCCATC CAGGTGGCCA ATCTCCTGCG
  781 GCTGTTCCAG ATCCCACAGA TCAGCTATGC CTCCACCAGT GCCAAGCTGA GTGACAAGTC
  841 CCGTTACGAT TACTTTGCTC GCACTGTGCC CCCAGACTTC TTCCAAGCCA AGGCCATGGC
  901 TGAGATTCTC CGCTTTTTCA ACTGGACATA TGTGTCTACG GTGGCATCTG AGGGTGACTA
  961 TGGTGAGACA GGCATTTGAG GCTTTCGAGC TCGAGGCTCG GGCACGCAAC ATCTGCGTGG
 1021 CCACTTCTGA AAGGTGGGCC GTTGCCATGA GCGCGCTGCC TTCGAAGGGC GTGGTGCGAG
 1081 CCCTGTTGCA GAACTCAGTG CCCGTGTGGC TGTGCTCTTC ACCGTTCGAA GAATGCCGGT
 1141 GAGCCTGCCT GGCAGCCACC AGGCCTCAAG GCAGCTACCA ATGGATGGAC CAAGCCGACG
1201 GCTGGGGGGT CCTG
//
Primer: T7_20bp
Sequence file: RDB13196_A5Cib.seq check
>13196_13196_A5Ci_T7_20mer_F03_18.ab1
    1 TGAAGCGGTG GCGGCGCTCT AGACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGGCACGAG
   61 GGTCGACGCG GCCGCTTTTT TTTTTTTTTT TTTTTTTTAA ATAAAGACAC TTTATTTACA
  121 GGTAATACAA AATAAACCAC GGGACAAACT ACTGGGTAAG GAGGGGAGGG AATGATCCCC
  181 ACAGTAACCA TCCTCTCTAT CCCAGAGTAA CTTATAAAAA ATTAAAAAGG CCTGGGGCAG
  241 GGGCTGGTCT GGAAGCACCT TTGCATGGGG GTTGGGCCTG TCGTCCTCAG CCACACTGTT
  301 CTAGAACACA GTAGACACTG GTCATCTCCC CCTCTGAGTC CCAGGCCTGG CAAACACGGG
  361 GCATAGCTAC AAGGACAGTT ACTGGCCATT GCAGAGGCTC CCCAGCTAAG GACTCTGGCT
  421 TCTGACCTAA AGGGCTGGGA ATCCAGGAGG TGGGTAAGTG GCTCTGGGGG CAGGCATGGG
  481 GTGGGTGTTA TTGCAGTGCT CCCAGCCAAC TTCCTCCTGG TCGTGGATGA GGGTCTATGC
  541 TGGGTTTGGG AGCAGCCATG TCAGGGCAGG AGTGTGGGAT CTTCAAAGCG ACGACGTTGT
  601 TGAGTCCACC ACCTCACGGC CGTTGCAAAC AGTGGGAACA AACTGGGATC CAGACCCTTG
  661 ACCAAGGTTG GCGCTGGCCC TGGGGGCAGC GCTGCCAAAG CGGCTGGTAG GTGCCCGGTG
  721 GCTCACCACA TTCTTCTGTG GCTGGAAAAG GATGATGTGC AACTTGGGTG CAAAGAGGCA
  781 GCCAAGCACC ACAGAGCCAC TGAGGCTGAC GGACACGCAC ATCGTGGTGG TCTGCACCCG
  841 ATAATCACTG GAGGTGACAT AGAAGATAGG AAGGAAAGCC AGCCAGATGA TGCAGGTGGT
  901 GTACATGGTG AAGCCGATGA ACTTGGCTTC GTTGAAGTTC TCCGGGCACT TGCGGGTCTT
  961 GAAGGCATAG AGCGTGCAGA GCGCGGATGA GGGAGCACAT TGTAGGCCAG AGAGGCCGAG
 1021 CATGCTCGCG TCACGGTGGA TTACAGCGCA ATGGTCACCC ACATTCCGAC CGATTCAATG
1081 GACTGATCTC CGTTGCCTGG TTGCCAAGAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tanabe, Y., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649. [link to RRC of NBRP]
user_report Onishi, T., Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. J. Physiol. 597 (13): 3441-3455 (2019). PMID 31087329. [link to RRC of NBRP]