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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmGR 2

Plasmic clone of rat mGluR2 cDNA

Catalog number RDB13196
Resource name pmGR 2
Clone info. Plasmid clone of rat mGluR2 cDNA. cDNA fragment 8 to 3,304nt of NM_001105711.1 (CDS, 199-2.817) is cloned. See Fig.1a of Tanabe, Y., Neuron 8 (1), 169-179 (1992).
Comment Known difference: C to T substitution at 73nt in 5' UTR of NM_001105711.1.
Vector backbone pBluescriptII KS(+) (Plasmid)
Gene/insert name Rat mGluR2 cDNA
Reference of insert sequence M92075.1 (DDBJ accession number)
Depositor|Developer Nakanishi, Shigetada |
Other clones in our bank
External Database
Rat mGluR2
          Reference sequence
            NM_001105711.1
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Neuron 8: 169-179, 1992) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Neuron 8: 169-179, 1992)。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13196 pmGR 2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pmGR 2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13196).

          Reference section:

          Tanabe, Y., Masu, M., Ishii, T., Shigemoto, R., Nakanishi, S., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13196_A5Cip1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Reverse2
          Sequence file: RDB13196_A5Cia.seq check
          >RDB13196_A5Ci_1_reverse2_H11_08_ABI08.ab1
              1 CGGTTTACCG GGATTACGGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
             61 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCGGCACG
            121 AGGCGAGAGA GCCAGCAAGC GGGAACCGGA GCCCCGCGCC AGAGCTGGGC GCGCCAGGAG
            181 CTGACCCTCT CCTCATCTCT CTTCCTGTCT GTCCTTTCCT GGCCCCTGTT TCCTCCTCTC
            241 TTTGCCTTTG CTGCTTCTGT CCTCACCCCT AGAAGACCCT GGTCTGCTGG ACCTAGCCAT
            301 CCCTTTTGGG GCCATGGAAT CACTGCTTGG GTTTCTGGCA CTGCTGCTGC TGTGGGGTGC
            361 CGTGGCCGAG GGCCCGGCCA AGAAGGTGCT GACCCTGGAG GGGGACCTGG TGCTGGGTGG
            421 GCTGTTCCCA GTGCACCAGA AGGGTGGCCC AGCCGAGGAG TGTGGACCTG TTAATGAGCA
            481 CCGAGGCATA CAGCGCCTAG AGGCTATGCT TTTTGCACTG GACCGCATCA ACCGCGACCC
            541 CCACCTGCTG CCTGGTGTGC GCTTGGGTGC GCACATCCTC GACAGCTGCT CCAAGGATAC
            601 ACACGCCCTG GAGCAGGCGC TGGACTTTGT GCGTGCCTCA CTCAGTCGTG GCGCTGACGG
            661 CTCACGCCAC ATCTGTCCTG ATGGCTCCTA TGCCACCCAC AGTGATGCTC CTACAGCTGT
            721 CACCGGTGTC ATTGGTGGCT CCTACAGTGA TGTCTCCATC CAGGTGGCCA ATCTCCTGCG
            781 GCTGTTCCAG ATCCCACAGA TCAGCTATGC CTCCACCAGT GCCAAGCTGA GTGACAAGTC
            841 CCGTTACGAT TACTTTGCTC GCACTGTGCC CCCAGACTTC TTCCAAGCCA AGGCCATGGC
            901 TGAGATTCTC CGCTTTTTCA ACTGGACATA TGTGTCTACG GTGGCATCTG AGGGTGACTA
            961 TGGTGAGACA GGCATTTGAG GCTTTCGAGC TCGAGGCTCG GGCACGCAAC ATCTGCGTGG
           1021 CCACTTCTGA AAGGTGGGCC GTTGCCATGA GCGCGCTGCC TTCGAAGGGC GTGGTGCGAG
           1081 CCCTGTTGCA GAACTCAGTG CCCGTGTGGC TGTGCTCTTC ACCGTTCGAA GAATGCCGGT
           1141 GAGCCTGCCT GGCAGCCACC AGGCCTCAAG GCAGCTACCA ATGGATGGAC CAAGCCGACG
          1201 GCTGGGGGGT CCTG
          //
          Primer: T7_20bp
          Sequence file: RDB13196_A5Cib.seq check
          >13196_13196_A5Ci_T7_20mer_F03_18.ab1
              1 TGAAGCGGTG GCGGCGCTCT AGACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGGCACGAG
             61 GGTCGACGCG GCCGCTTTTT TTTTTTTTTT TTTTTTTTAA ATAAAGACAC TTTATTTACA
            121 GGTAATACAA AATAAACCAC GGGACAAACT ACTGGGTAAG GAGGGGAGGG AATGATCCCC
            181 ACAGTAACCA TCCTCTCTAT CCCAGAGTAA CTTATAAAAA ATTAAAAAGG CCTGGGGCAG
            241 GGGCTGGTCT GGAAGCACCT TTGCATGGGG GTTGGGCCTG TCGTCCTCAG CCACACTGTT
            301 CTAGAACACA GTAGACACTG GTCATCTCCC CCTCTGAGTC CCAGGCCTGG CAAACACGGG
            361 GCATAGCTAC AAGGACAGTT ACTGGCCATT GCAGAGGCTC CCCAGCTAAG GACTCTGGCT
            421 TCTGACCTAA AGGGCTGGGA ATCCAGGAGG TGGGTAAGTG GCTCTGGGGG CAGGCATGGG
            481 GTGGGTGTTA TTGCAGTGCT CCCAGCCAAC TTCCTCCTGG TCGTGGATGA GGGTCTATGC
            541 TGGGTTTGGG AGCAGCCATG TCAGGGCAGG AGTGTGGGAT CTTCAAAGCG ACGACGTTGT
            601 TGAGTCCACC ACCTCACGGC CGTTGCAAAC AGTGGGAACA AACTGGGATC CAGACCCTTG
            661 ACCAAGGTTG GCGCTGGCCC TGGGGGCAGC GCTGCCAAAG CGGCTGGTAG GTGCCCGGTG
            721 GCTCACCACA TTCTTCTGTG GCTGGAAAAG GATGATGTGC AACTTGGGTG CAAAGAGGCA
            781 GCCAAGCACC ACAGAGCCAC TGAGGCTGAC GGACACGCAC ATCGTGGTGG TCTGCACCCG
            841 ATAATCACTG GAGGTGACAT AGAAGATAGG AAGGAAAGCC AGCCAGATGA TGCAGGTGGT
            901 GTACATGGTG AAGCCGATGA ACTTGGCTTC GTTGAAGTTC TCCGGGCACT TGCGGGTCTT
            961 GAAGGCATAG AGCGTGCAGA GCGCGGATGA GGGAGCACAT TGTAGGCCAG AGAGGCCGAG
           1021 CATGCTCGCG TCACGGTGGA TTACAGCGCA ATGGTCACCC ACATTCCGAC CGATTCAATG
          1081 GACTGATCTC CGTTGCCTGG TTGCCAAGAT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tanabe, Y., A family of metabotropic glutamate receptors. Neuron 8: 169-179 (1992). PMID 1309649.
          user_report Onishi, T., Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. J. Physiol. 597 (13): 3441-3455 (2019). PMID 31087329.

          2023.05.02

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