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HDAC1-pcDNA3.1 myc-His (#RDB13162)

Expression plasmid of human HDAC1

Clone info. Expression plasmid of human HDAC1.
Comment Expression was confirmed by the depositor with Western blotting. Known differences (at least): compared with NM_004964.2 ,T to C substitution at 380nt (Leu to Pro), C to T substitution at 483nt (His to His,synonym), and A to G substitution at 929nt (Lys to Arg).
Vector backbone pcDNA3.1-myc/His (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli); Neo^r (mammalian cells)
Gene/insert name human HDAC1 cDNA
Depositor|Developer Sahara, Hiroeki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tanabe, A., Gene 535 (1): 24-32, 2014)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tanabe, A., Gene 535 (1): 24-32, 2014)。

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RDB13162 HDAC1-pcDNA3.1 myc-His DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HDAC1-pcDNA3.1 myc-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13162).

Reference section:

Tanabe, A., Konno, J., Tanikawa, K., Sahara, H., Transcriptional machinery of TNF-alpha-inducible YTH domain containing 2 (YTHDC2) gene. Gene 535 (1): 24-32 (2014). PMID 24269672. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13162_A5C6p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB13162_A5C6a.seq check
>13162_13162_A5C6_CMV-Forward_F01_06_ABI08.ab1
    1 CTTAACCAAC CTCCGGCCCC CATTGACGGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG
  181 ATCCACTAGT CCAGTGTGGT GGAATTCATG GCGCAGACGC AGGGCACCCG GAGGAAAGTC
  241 TGTTACTACT ACGACGGGGA TGTTGGAAAT TACTATTATG GACAAGGCCA CCCAATGAAG
  301 CCTCACCGAA TCCGCATGAC TCATAATTTG CTGCTCAACT ATGGTCTCTA CCGAAAAATG
  361 GAAATCTATC GCCCTCACAA AGCCAATGCT GAGGAGATGA CCAAGTACCA CAGCGATGAC
  421 TACATTAAAT TCTTGCGCTC CATCCGTCCA GATAACATGT CGGAGTACAG CAAGCAGATG
  481 CAGAGATTCA ACGTTGGTGA GGACTGTCCA GTATTCGATG GCCCGTTTGA GTTCTGTCAG
  541 TTGTCTACTG GTGGTTCTGT GGCAAGTGCT GTGAAACTTA ATAAGCAGCA GACGGACATC
  601 GCTGTGAATT GGGCTGGGGG CCTGCATCAT GCAAAGAAGT CCGAGGCATC TGGCTTCTGT
  661 TACGTCAATG ATATCGTCTT GGCCATCCTG GAACTGCTAA AGTATCACCA GAGGGTGCTG
  721 TACATTGACA TTGATATTCA CCATGGTGAC GGCGTGGAAG AGGCCTTCTA CACCACGGAC
  781 CGGGTCATGA CTGTGTCCTT TCATAAGTAT GGAGAGTACT TCCCAGGAAC TGGGGACCTA
  841 CGGGATATCG GGGCTGGCAA AGGCAAGTAT TATGCTGTTA ACTACCCGCT CCGAGACGGG
  901 ATTGATGACG AGTCCTATGA GGCCATTTTT CAAGCCGGTC ATGTCCAAAG TAATGGAGAT
  961 GTTCCCAGCC TAGTGCGGGT GGTCTTTACA GTGTGGCTCA GACTCCCTAT CTGGGGATCG
 1021 GTTAGGGTTG CTTCAATCTA ACTATCAAAG ACACGCCCAA GTGTGTGGAT TGTCAGGAGC
1081 TTTAAACTTG CCTATGCTGA TGCCTGGAGC CGGTGGTTAC ACACCATTTC CGATTAGC
//
Primer: BGH_rev ()
Sequence file: RDB13162_A5C6b.seq check
>13162_13162_A5C6_BGH_reverse_1_B12_06.ab1
    1 GTTCTCATGG TGATGGTGAT GATGACCGGT ATGCATATTC AGATCCTCTT CTGAGATGAG
   61 TTTTTGTTCG AAGGGCCCTC TAGACTCGAG CGGCCGCCAG GCCAACTTGA CCTCCTCCTT
  121 GACCCCTTTG GCTTCTGGCT TCTCCTCCTT GGTTTTCTCC TCTTCGGTGA CTTCTTTCTT
  181 CTCCTCTGGG TCTTTCTCTT TTTCATCCTC TGTTTTGACT CTCTTGGCTT TTTTGAAGTT
  241 GGAAGAGTTC TTGCGGCCCC CCTCTCCCTC CTCTTCAGAA TCGGAGAACT CTTCCTCACA
  301 GGCAATTCGT TTGTCAGAGG AGCAGATCGA GATGCGCTTG TCAGGGTCGT CTTCGTCCTC
  361 ATCGCCACTC TCCTCAGGGA TGGCGTCCTC AGGAATCGCC TGCATTTGGA CCCCAGGTGC
  421 GTGCGGCAGC ATTCTAAGGT TCTCAAACAG TCGCTGTTTG ATCTTCTCCA GGTACTCATT
  481 CGTGTTCTGG TTAGTCATAT TGGAAGGACT GATGTGGAGC TTGAAATCTG GTCCAAAGTA
  541 TTCAAAGTAG TCATTGTATG GAAGCTCATT AGGGATCTCC GTATCCAGGG CCACAGCTGT
  601 CTCATATGTC CAGCACCGGG CAACGTTACG AATGGTGTAA CCACCGCCTC CCAGCATCAG
  661 CATAGGCAGG TTAAAGCTCC TGACAAATTC CACACACTTG GCGTGTCCTT TGATAGTTAG
  721 ATTGAAGCAA CCTAACCGAT CCCCAGATAG GGAGTCTGAG CCACACTGTA AGACCACCGC
  781 ACTAGGCTGG AACATCTCCA TTACTTTGGA CATGACCGGC TTGAAAATGG CCTCATAGGA
  841 CTCGTCATCA ATCCCGTCTC GGAGCGGGTA GTTAACAGCA TAATACTTGC CTTTGCCAGC
  901 CCCGATATCC CGTAGTCCCC AGTTCCTGGG AAGTACTCTC CATACTTATG AAGGACACAG
  961 TCATGACCCG GGTCCGTGGT GTAGAACGCC TCTTCACGCG TCACATGTGA ATATCATGTC
 1021 ATGTACAGCA ACCTCTGTTG ATACTTTAGC AGTCAGCATT GATAGACGAT TCATTGACGT
1081 ACAGAAGTAG ATGCCTCGGA CGTTC
//
Primer: pBABE3' ()
Sequence file: RDB13162_A5C6c.seq check
>13162_13162_A5C6_pBABE3'_1_B06_06.ab1
    1 CTCCGCGCTT AGTTTTGTTA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA
   61 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA
  121 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG
  181 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
  241 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG
  301 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA
  361 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG
  421 GCGCGTGGGG ATACCCCCTA GAGCCCCAGC TGGTTCTTTC CGCCTCAGAA GCCATAGAGC
  481 CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC
  541 CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT
  601 TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG
  661 GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGGGTTTAAA
  721 CTCAATGGTG ATGGTGATGA TGACCGGTAT GCATATTCAG ATCCTCTTCT GAGATGAGTT
  781 TTTGTTCGAA GGGCCCTCTA GACTCGAGCG GCCGCCAGGC CAACTTGACC TCCTCCTTGA
  841 CCCCTTTGGC TTCTGGCTTC TCCTCCTTGG TTTTCTCCTC TTCGGTGACT TCTTTCTTCT
  901 CCTCTGGGTC TTTCTCTTTT TTCATCCTCT GTTTTGACTC TCTTGGCTTT TTTTGAAGTT
  961 GGAAGAGTTC TTGCGGCCCC CCTCTCCCTC CTCTTCAGAA TCGGGAGAAC TCTTTCCTCA
 1021 CAGGCCAATT CGTTTGTCAG AGGAGCAGAT CGAGATGCGC TTGTCAAGAT CGTCTTTCGG
 1081 TCCTCAATCG TACTCTCTTC AGATTGGCGT TCTTCAAGAT TCGCCTTGCC ATTTTGGGCA
1141 C
//
Primer: SV40pro_F ()
Sequence file: RDB13162_A5C6d.seq check
>13162_13162_A5C6_SV40pro_F_1_C06_09.ab1
    1 CCTGTCTTGA CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCTTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC
  961 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCCT TCTATGAAGG TTGGGGCTTC
 1021 GGAATCGGTT TTCCGGGACG CCCGGCTGGA TGATCCCTCC AGCCGCCGGG GATCTCATGC
 1081 TGGGAGTTCT TCGGTCTACC CCAACCTGTT ATTGCCAGCT ATATTGGGTT AACAAAACTT
1141 ACAAGGGCCA CAATTAAGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tanabe, A., Transcriptional machinery of TNF-alpha-inducible YTH domain containing 2 (YTHDC2) gene. Gene 535 (1): 24-32 (2014). PMID 24269672. [link to RRC of NBRP]