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PB-TAC (#RDB13131)

piggyBac transposon system for dox-inducible gene expression (with mCherry reporter)


Drawn by SnapGene® software
Sequence (full) RDB13131hts01.seq
Publication Kim, S.I., Stem Cell Reports. 4 (4): 727-743 (2015). [link to RRC of NBRP]
Test sheet RDB13131C0p1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name KW016
Clone info. piggyBac transposon system for dox-inducible gene expression (with mCherry reporter).
Comment Expression was confirmed by the depositor with human and mouse ES/iPS cells, differentiated cells, and common cell lines (HeLa, HEK293T).
Relation: KW025 (RDB13132), KW031 (RDB13133), KW036 (RDB13134), KW252 (RDB13135), KW443 (RDB13136), KW505 (RDB13137), KW513 (RDB13138) (derivatives containing iPS cell reprogramming cassettes).
Vector backbone Plasmid
Size of vector backbone 3.0 kb
Selectable markers Amp^r, Cm^r
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer CiRA, Kyoto Univ. | Woltjen, Knut |

Distribution information

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This RESOURCE contains a piggyBac® transposon. Limited Use License Agreement (LULA) [Word] is also required.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution For provision of this material, prior permission by the Depositor (Kyoto University cira-keiyaku@cira.kyoto-u.ac.jp) is required. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Kim, S.I. et al., Stem Cell Reports 4 (4): 727-743, 2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required. Use DB3.1(Invitrogen) or equivalent to amplify this clone.
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必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
piggyBac®トランスポゾンを内包するバイオリソースです。Limited Use License Agreement (LULA) [Word]の提出をお願いします。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、事前に寄託者 (連絡先：京都大学 cira-keiyaku@cira.kyoto-u.ac.jp) から提供承諾を得る。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Kim, S.I. et al., Stem Cell Reports 4 (4): 727-743, 2015)。学術機関の学術研究に限る。
備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。このクローンの増殖にはDB3.1(Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13131 PB-TAC DNA solution

How to cite this biological resource

Materials & Methods section:

The PB-TAC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13131).

Reference section:

Kim, S.I., Oceguera-Yanez, F., Hirohata, R., Linker, S., Okita, K., Yamada, Y., Yamamoto, T., Yamanaka, S., Woltjen, K., KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 4 (4): 727-743 (2015). PMID 25772473. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kim, S.I., KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 4 (4): 727-743 (2015). PMID 25772473. [link to RRC of NBRP]