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pROSA-floxNeo-Fucci2a

Fucci2a cell cycle reporter. Targeting vector for R26 locus

Catalog number RDB13081
Resource name pROSA-floxNeo-Fucci2a
Clone info. Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120). Targeting vector for R26 locus. Fucci2aR ROSA26 knock-in mice (cat.#RBRC06511) is available from the Experimental Animal Division.
Vector backbone pCAGiP (Plasmid)
Selectable markers Ampicillin
Gene/insert name Human Cdt1 cDNA Human Gem cDNA Corynebacterium diphtheriae Diphtheria toxin A genomic DNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Mort, Richard |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) | Corynebacterium diphtheriae tox (KEGG orthology K00776) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
(3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
(4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
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RDB13081 pROSA-floxNeo-Fucci2a DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0055e (English text)
Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13081_A5Ajp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: lox P,NeoR
Sequence file: RDB13081_A5Ajj.seq check
>13081_13081_A5Aj_pAxCA_F1_8_B10_04.ab1
    1 CGTCCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGATATC AAGCTTATCG AGCGGCCGCT
  121 CTAGAACTAG TGGATCCATA ACTTCGTATA GCATACATTA TACGAAGTTA TCCCAGATCC
  181 CCCGGGCTGC AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
  241 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
  301 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
  361 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
  421 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
  481 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
  541 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
  601 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
  661 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
  721 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
  781 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
  841 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
  901 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
  961 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG
 1021 AAATGACCGA CCCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTT CCACCGCGCG
 1081 TTCTATGAAA GATGGGGCCT TCGTATCGGT TTCAGGAACG ATGCCTGGAT GATTCCCTCC
1141 AGCCGGCGGG GATTCTCTCT CAAG
//
Primer: pAxCALNLF1
Region: SV40 pA,lox P,mCherry-hCdt1_30_120 5'
Sequence file: RDB13081_A5Ajk.seq check
>13081_13081_A5Aj_pAxCALNL_F1_8_B01_04.ab1
    1 ATTATGTATC TTATCATGTC TGGATCTCCC GATATCCCCA TAACTTCGTA TAGCATACAT
   61 TATACGAAGT TATGGATCCC CCGGGCTGCA GGAATTCCTC GACACGCGTA TGGTGAGCAA
  121 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  181 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  241 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
  301 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  361 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
  421 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
  481 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
  541 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
  601 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
  661 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
  721 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
  781 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC
  841 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG
  901 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG
  961 CAGAGACTGC GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCCG AGGCCCCAGA
 1021 CATCCCAGCC TGCCCCTTCT CCGGGCCAGA GATAAAGAAT CCACCCCCGG CAGCAGTCAG
 1081 CGCTCCCACC TTGAACATCC GCGCAGACAG ACATCAATTC ATGAGGTCGT GCCTTCCTCC
1141 TTCTTTTCAC CTGTATG
//
Primer: PGK_N_rev
Region: ROSA_3 5'
Sequence file: RDB13081_A5Ajl.seq check
>13081_13081_B0I8F_PGK_N_R_G06_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TTATCGATAC CGTCGACGGT
   61 ATCGATAAGC TAGATGTCCT GAAATATTTC TATCAGAACA AGGTAGTATA AAGCTGGTAG
  121 GTATACAAAA CGCTAGACTA GTTTCTATCC CTGACCCTTA ATCTGCTAGT ATATCCGTAG
  181 GAAGTTGCTT AAGTGCCACT AGTACCAACA GCCTCTGTTA GAGTGAACAG TAGATTTTAA
  241 TGAAGGGCCA ATAAAGACCA CTTAAGACTA CTGACTGAAT TAAAAATCCA CCCAGTGGAG
  301 TTAGTATTGG TTATAGTTAT ATAACAAAAT ATCCAACTTA GCCAGGCGTG GTGGTGCACG
  361 CCTTTAATCC CGGCACTCAG GAGGCAGAGG CAGGCGGATT TCTGAGTTCG AGGCCAGCCT
  421 GGTCTACAAA GTGAGTTCCA GGACAGCCAG GGCTATACAG AGAAACCCTG TCTCGAAAAA
  481 CCAAAAAAAA AAAAAAAAAG GAAAAAAAAT ATGGCTTGAA AACATGCTAT TGTTGTCAAA
  541 AGGAGATCCA AACAAGTATT TCTTTGAACA AAAATTATGT AGAAAACAGG GTGATAAAGA
  601 ACAAGGGCTT TTTAAAAAAG GCCAGCATTA TTTGAGATAT ACAAGTGACT CCAATTCAAG
  661 CCTAAAGGCC ACTCAATGCT CACTAACAGT GCTAATCANA ATCAATTCCT TAGCCCAGTC
  721 NAAAATAGTC CTGAATATCT TCTGGATNAA CNTGTCCTCC NACATATNAA NNTANTATAC
  781 ACTAANNAAA CGTCTCNACT TNNNNGTGAA ATGCTTGACN CCTANACTTN NGACCNNNNN
  841 AANTGCTCTA NNNGTAGGGT TACTANGNNA NANANTCNTG NNNNGNNAAN NNCNNNTATN
  901 NNANTNNNNN NNAANNNNAN AANNNGNAAA ANTGANNTNN NTTANNTNNN NTTTNNAAAN
961 NTNAAAANNN NNNNNNN
//
Primer: pUC-M13_-100
Region: ROSA_5 3'
Sequence file: RDB13081_A5Ajm.seq check
>13081_13081_B0I8F_pUC-M13_-100_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNN GGGTTTTCCC NGTCACGACG TTGTAAAACG
   61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGC
  121 AGGCCCTCCG AGCGTGGTGG AGCCGTTCTG TGAGACAGCC GGGTACGAGT CGTGACGCTG
  181 GAAGGGGCAA GCGGGTGGTG GGCAGGAATG CGGTCCGCCC TGCAGCAACC GGAGGGGGAG
  241 GGAGAAGGGA GCGGAAAAGT CTCCACCGGA CGCGGCCATG GCTCGGGGGG GGGGGGGCAG
  301 CGGAGGAGCG CTTCCGGCCG ACGTCTCGTC GCTGATTGGC TTCTTTTCCT CCCGCCGTGT
  361 GTGAAAACAC AAATGGCGTG TTTTGGTTGG CGTAAGGCGC CTGTCAGTTA ACGGCAGCCG
  421 GAGTGCGCAN NCGCCGGCAG CCTCGCTCTG CCCACTGGGT GGGGCGGGAG GTAGGTGGGN
481 N
//
Primer: T3
Region: bGHpA,Diphtheria toxin A 3'
Sequence file: RDB13081_A5Ajn.seq check
>13081_13081_A5Aj_T3_8_C01_07.ab1
    1 ATGTCGGGAC CCCCTCGAGC CCCAGCTGGT TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC
   61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC
  121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA
  181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA
  241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGGATC
  301 TGCATTCCAC CACTGCTCCC ATTCATCAGT TCCATAGGTT GGAATCTAAA ATACACAAAC
  361 AATTAGAATC AGTAGTTTAA CACATTATAC ACTTAAAAAT TTTATATTTA CCTTAGAGCT
  421 TTAAATCTCT GTAGGTAGTT TGTCCAATTA TGTCACACCA CAGAAGTAAG GTTCCTTCAC
  481 AAAGAGATCG CCTGACACGA TTTCCTGCAC AGGCTTGAGC CATATACTCA TACATCGCAT
  541 CTTGGCCACG TTTTCCACGG GTTTCAAAAT TAATCTCAAG TTCTACGCTT AACGCTTTCG
  601 CCTGTTCCCA GTTATTAATA TATTCAACGC TAGAACTCCC CTCAGCGAAG GGAAGGCTGA
  661 GCACTACACG CGAAGCACCA TCACCGAACC TTTTGATAAA CTCTTCCGTT CCGACTTGCT
  721 CCATCAACGG TTCAGTGAGA CTTAAACCTA ACTCTTTCTT AATAGTTTCG GCATTATCCA
  781 CTTTTAGTGC GAGAACCTTC GTCAGTCCTG GATACGTCAC TTTGACCACG CCTCCAGCTT
  841 TTCCAGAGAG CGGGTTTTCA TTATCTACAG AGTATCCCGC AGCGTCGTAT TTATTGTCGG
  901 TACTATAAAA CCCTTTCCAA TCATCGTCAT AATTTCCTTG TGTACCAGAT TTTGGCTTTT
  961 GTATACCTTT TTTGAATGGA AATCTACATA ACCAGGGTTT AGTCCCGTGG TACGAAGAAA
 1021 AGTTTTCCAT CACAAAAGAT TTAGAGATCA CACATCATCA GGATCATGCG AGACTGCAGT
 1081 CGAAGGACAG AGATGAGGAA GAGAGAACAG CGCGCCAGGA CGTGCGCCTT TTTGAAGCGC
1141 GGGTGCA
//
Primer: CMV_en_R
Region: ROSA_3 3'
Sequence file: RDB13081_A5Ajo.seq check
>13081_13081_A5Aj_CMV_en_R_8_A10_01.ab1
    1 GTCTTTATTG ATTACTATTA ATAACTAGTC AATAATCAAT GTCGCACTTA ATTAAACGCG
   61 CCGGCTAGAA GATGGGCGGG AGTCTTCTGG GCAGGCTTAA AGGCTAACCT GGTGTGTGGG
  121 CGTTGTCCTG CAGGGGAATT GAACAGGTGT AAAATTGGAG GGACAAGACT TCCCACAGAT
  181 TTTCGGTTTT GTCGGGAAGT TTTTTAATAG GGGCAAATAA GGAAAATGGG AGGATAGGTA
  241 GTCATCTGGG GTTTTATGCA GCAAAACTAC AGGTTATTAT TGCTTGTGAT CCGCCTCGGA
  301 GTATTTTCCA TCGAGGTAGA TTAAAGACAT GCTCACCCGA GTTTTATACT CTCCTGCTTG
  361 AGATCCTTAC TACAGTATGA AATTACAGTG TCGCGAGTTA GACTATGTAA GCAGAATTTT
  421 AATCATTTTT AAAGAGCCCA GTACTTCATA TCCATTTCTC CCGCTCCTTC TGCAGCCTTA
  481 TCAAAAGGTA TTTTAGAACA CTCATTTTAG CCCCATTTTC ATTTATTATA CTGGCTTATC
  541 CAACCCCTAG ACAGAGCATT GGCATTTTCC CTTTCCTGAT CTTAGAAGTC TGATGACTCA
  601 TGAAACCAGA CAGATTAGTT ACATACACCA CAAATCGAGG CTGTAGCTGG GGCCTCAACA
  661 CTGCAGTTCT TTTATAACTC CTTAGTACAC TTTTTGTTGA TCCTTTGCCT TGATCCTTAA
  721 TTTTCAGTGT CTATCACCTC TCCCGTCAGG TGGTGTTCCA CATTTGGGCC TATTCTCAGT
  781 CCAGGGAGTT TTACAACAAT AGATGTATTG AGAATCCAAC CTAAAGCTTA ACTTTCCACT
  841 CCCATGAATG CCTCTCTCCT TTTTCTCCAT TTATAAACTG AGCTATTAAC CATTAATGGT
  901 TTCCAGGTGG ATGTCTCCTC CCCCAATATT ACCTGATGTA TCTTACATAT TGCCAGGCTG
  961 ATATTTTAAA GACATTAAAA GGTATATTTC ATTATTGAGC CACATGGGTA TTGATTACTG
 1021 CTTTACTAAA ATTTTGTCAT TGTACACATC TGTAAAAGGT GGTTTCCCTT TTGGGAATGC
 1081 AAAGTTCAGG TGATTGATGT CTTTCTGACC TTACGTCTGT GAGCCTGCAT TTTTTTCTAA
1141 TTTAAGCAGG GTGCTTTTCT T
//
Primer: EGFP-C
Region: mVenus-hGeminin1_110 3',bGHpA,ROSA_5
Sequence file: RDB13081_A5Ajp.seq check
>13081_13081_A5Aj_EGFP_C_8_D01_10.ab1
    1 GCGTACTCTC GGCATGGACG AGCTGTACAA GGGATATCCA TCACACTGGC GGCCGCTCGA
   61 GATGAATCCC AGTATGAAGC AGAAACAAGA AGAAATCAAA GAGAATATAA AGAATAGTTC
  121 TGTCCCAAGA AGAACTCTGA AGATGATTCA GCCTTCTGCA TCTGGATCTC TTGTTGGAAG
  181 AGAAAATGAG CTGTCCGCAG GCTTGTCCAA AAGGAAACAT CGGAATGACC ACTTAACATC
  241 TACAACTTCC AGCCCTGGGG TTATTGTCCC AGAATCTAGT GAAAATAAAA ATCTTGGAGG
  301 AGTCACCCAG GAGTCATTTG ATCTTATGAT TAAAGAAAAT CCATCCTCTC AGTATTGGAA
  361 GGAAGTGGCA GAAAAACGGA GAAAGGCGCT GTAAGGTACC ACATGTGGCG CGCGCGCCTT
  421 CGATGATCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
  481 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
  541 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
  601 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT
  661 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCTGCA GCCCAAGCTT GGGCTGCAGG
  721 GCGCGCCCGA TTAAGGCTAG AAAGACTGGA GTTGCAGATC ACGAGGGAAG AGGGGGAAGG
  781 GATTCTCCCA GGCCCAGGGC GGTCCTCAGA AGCCAGGAGG CAGCAGAGAA CTCCCAGAAA
  841 GGTATTGCAA CACTCCCCTC CCCCCTCCGG AGAAGGGTGC GGCCTTCTCC CCGCCTACTC
  901 CACTGCAGCT CCCTTACTGA TAACAACTCA GAGCGACTTT GGGAGAGCAA GTGCTTCCTG
  961 CCTCCAAAAC AGCCCAACTG AGCCCTCGCT CCTTTCCTCC ACTCCCCGGA GTGCGCGATG
 1021 GGAGGTCTGG CTCAGCACGC CCCTCTTGAG GCAACTCAAG TCGGGAAACG TGCTGCACCC
 1081 GCCCCGCAGC GCTCAGCCTT ACTGACGTTC CGCCCCAGCG CGCGCATCTG ACCACGTTGC
1141 CGCCAAGGGG GACGTC
//
Primer: EGFP-N
Region: mVenus-hGeminin1_110 5',T2A,mCherry-hCdt1_30_120
Sequence file: RDB13081_A5Ajq.seq check
>13081_13081_A5Aj_EGFP_N_8_A01_01.ab1
    1 GGCCCGGCGA CGCTCCTCGC CCTTGCTCAC CATGGGTCCA GGATTCTCTT CGACATCGCC
   61 ACAAGTGAGA AGAGAGCCAC GGCCTTCCAA TTGGATGGTG TCCTGGTCCT GCGCGGATGT
  121 CAGGTGGGGC GGCTGACCTG CTGCCGGGGT GGATTTCTTT ATCTTCTGGC CCGGAGAAGG
  181 GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT ACTGGGGCTG GAAACCTCGT CCACCGACAG
  241 CCGCAGTCTC CTGCGGGCCG GTGGCCTGGC CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG
  301 GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG
  361 GCTGGGCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG TACAGCTCGT CCATGCCGCC
  421 GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG
  481 GGAGGTGATG TCCAACTTGA TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT
  541 GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT
  601 CTGCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC
  661 CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG
  721 CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC
  781 GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA AGGACAGCTT
  841 CAAGTAGTCG GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
  901 GAGGGGACAG GATGTCCCAG GCGAAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG
  961 GCGGTCTGGG TGCCCTCGTA GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
 1021 CGTTCACGGG AGCCCTCCCA TGTGCACCTT TGAGCGCATG AACTCATGAA TGATGGCATG
 1081 ATATCTCCTC GCCTGCTCAC ATACGCGTGT CGAGAGTTCC CTGCCAGCCC GGGGGGAATC
1141 CAA
//

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References

Original, user report and related articles

original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.
reference Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
reference Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
reference Glover, J.D., Maintenance of distinct melanocyte populations in the interfollicular epidermis. Pigment Cell Melanoma Res. 28 (4): 476-480 (2015). PMID 25847135.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl