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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pROSA-floxNeo-Fucci2a

Fucci2a cell cycle reporter. Targeting vector for R26 locus

Catalog number RDB13081
Resource name pROSA-floxNeo-Fucci2a
Clone info. Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120). Targeting vector for R26 locus. Fucci2aR ROSA26 knock-in mice (cat.#RBRC06511) is available from the Experimental Animal Division.
Vector backbone pCAGiP (Plasmid)
Selectable markers Ampicillin
Gene/insert name Human Cdt1 cDNA Human Gem cDNA Corynebacterium diphtheriae Diphtheria toxin A genomic DNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Mort, Richard |
Other clones in our bank

External Database
Human Cdt1 Human Gem Corynebacterium diphtheriae Diphtheria toxin A Discosoma sp. RFP
    Aequorea victoria GFP

      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
      2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
      (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
      (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
      Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
      Ordering Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section.
      Order form [Credit Card Payment]  [Bank Transfer Payment]
      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      Approval form [Word] by Dr. Atsushi Miyawaki
      Remarks
      UCSD's MTA is also required. Please visit Additional Forms section.
      Please obtain written approval of Dr. Atsushi Miyawaki.
      提供案内 (日本国内) [open/close]

      提供条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
      2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
      (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
      (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
      付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
      宮脇敦史博士による提供承諾書 [Word]
      備考 タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。提供条件の言語は英語のみ。
      別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。
      宮脇敦史博士の書面による提供承諾が必要です。

      Catalog # Resource name Shipping form Fee (non-profit org.)
      RDB13081 pROSA-floxNeo-Fucci2a DNA solution

      Ordering Information [in Japanese] [in English]
      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Electronic file

      Original reference

      original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

      Further references such as user reports and related articles (go to bottom)

      Featured content

      Featured content Dnaconda's recommendation EXR0055e (English text)
      Featured content Fucci-related materials (en) (English text)
      Featured content Fucci-related materials (ja) (Japanese text)

      Sequence information

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB13081_A5Ajp1-2.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: pAxCA_F1
      Region: lox P,NeoR
      Sequence file: RDB13081_A5Ajj.seq check
      >13081_13081_A5Aj_pAxCA_F1_8_B10_04.ab1
          1 CGTCCTGCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
         61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGATATC AAGCTTATCG AGCGGCCGCT
        121 CTAGAACTAG TGGATCCATA ACTTCGTATA GCATACATTA TACGAAGTTA TCCCAGATCC
        181 CCCGGGCTGC AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
        241 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
        301 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
        361 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
        421 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
        481 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
        541 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
        601 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
        661 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
        721 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
        781 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
        841 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
        901 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
        961 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG
       1021 AAATGACCGA CCCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTT CCACCGCGCG
       1081 TTCTATGAAA GATGGGGCCT TCGTATCGGT TTCAGGAACG ATGCCTGGAT GATTCCCTCC
      1141 AGCCGGCGGG GATTCTCTCT CAAG
      //
      Primer: pAxCALNLF1
      Region: SV40 pA,lox P,mCherry-hCdt1_30_120 5'
      Sequence file: RDB13081_A5Ajk.seq check
      >13081_13081_A5Aj_pAxCALNL_F1_8_B01_04.ab1
          1 ATTATGTATC TTATCATGTC TGGATCTCCC GATATCCCCA TAACTTCGTA TAGCATACAT
         61 TATACGAAGT TATGGATCCC CCGGGCTGCA GGAATTCCTC GACACGCGTA TGGTGAGCAA
        121 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
        181 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
        241 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
        301 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
        361 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
        421 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
        481 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
        541 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
        601 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
        661 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
        721 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
        781 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC
        841 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGCAG
        901 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGCCAGGC CACCGGCCCG
        961 CAGAGACTGC GGCTGTCGGT GGACGAGGTT TCCAGCCCCA GTACCCCCCG AGGCCCCAGA
       1021 CATCCCAGCC TGCCCCTTCT CCGGGCCAGA GATAAAGAAT CCACCCCCGG CAGCAGTCAG
       1081 CGCTCCCACC TTGAACATCC GCGCAGACAG ACATCAATTC ATGAGGTCGT GCCTTCCTCC
      1141 TTCTTTTCAC CTGTATG
      //
      Primer: PGK_N_rev
      Region: ROSA_3 5'
      Sequence file: RDB13081_A5Ajl.seq check
      >13081_13081_B0I8F_PGK_N_R_G06_07_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TTATCGATAC CGTCGACGGT
         61 ATCGATAAGC TAGATGTCCT GAAATATTTC TATCAGAACA AGGTAGTATA AAGCTGGTAG
        121 GTATACAAAA CGCTAGACTA GTTTCTATCC CTGACCCTTA ATCTGCTAGT ATATCCGTAG
        181 GAAGTTGCTT AAGTGCCACT AGTACCAACA GCCTCTGTTA GAGTGAACAG TAGATTTTAA
        241 TGAAGGGCCA ATAAAGACCA CTTAAGACTA CTGACTGAAT TAAAAATCCA CCCAGTGGAG
        301 TTAGTATTGG TTATAGTTAT ATAACAAAAT ATCCAACTTA GCCAGGCGTG GTGGTGCACG
        361 CCTTTAATCC CGGCACTCAG GAGGCAGAGG CAGGCGGATT TCTGAGTTCG AGGCCAGCCT
        421 GGTCTACAAA GTGAGTTCCA GGACAGCCAG GGCTATACAG AGAAACCCTG TCTCGAAAAA
        481 CCAAAAAAAA AAAAAAAAAG GAAAAAAAAT ATGGCTTGAA AACATGCTAT TGTTGTCAAA
        541 AGGAGATCCA AACAAGTATT TCTTTGAACA AAAATTATGT AGAAAACAGG GTGATAAAGA
        601 ACAAGGGCTT TTTAAAAAAG GCCAGCATTA TTTGAGATAT ACAAGTGACT CCAATTCAAG
        661 CCTAAAGGCC ACTCAATGCT CACTAACAGT GCTAATCANA ATCAATTCCT TAGCCCAGTC
        721 NAAAATAGTC CTGAATATCT TCTGGATNAA CNTGTCCTCC NACATATNAA NNTANTATAC
        781 ACTAANNAAA CGTCTCNACT TNNNNGTGAA ATGCTTGACN CCTANACTTN NGACCNNNNN
        841 AANTGCTCTA NNNGTAGGGT TACTANGNNA NANANTCNTG NNNNGNNAAN NNCNNNTATN
        901 NNANTNNNNN NNAANNNNAN AANNNGNAAA ANTGANNTNN NTTANNTNNN NTTTNNAAAN
      961 NTNAAAANNN NNNNNNN
      //
      Primer: pUC-M13_-100
      Region: ROSA_5 3'
      Sequence file: RDB13081_A5Ajm.seq check
      >13081_13081_B0I8F_pUC-M13_-100_C05_08_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNN GGGTTTTCCC NGTCACGACG TTGTAAAACG
         61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGCGGC
        121 AGGCCCTCCG AGCGTGGTGG AGCCGTTCTG TGAGACAGCC GGGTACGAGT CGTGACGCTG
        181 GAAGGGGCAA GCGGGTGGTG GGCAGGAATG CGGTCCGCCC TGCAGCAACC GGAGGGGGAG
        241 GGAGAAGGGA GCGGAAAAGT CTCCACCGGA CGCGGCCATG GCTCGGGGGG GGGGGGGCAG
        301 CGGAGGAGCG CTTCCGGCCG ACGTCTCGTC GCTGATTGGC TTCTTTTCCT CCCGCCGTGT
        361 GTGAAAACAC AAATGGCGTG TTTTGGTTGG CGTAAGGCGC CTGTCAGTTA ACGGCAGCCG
        421 GAGTGCGCAN NCGCCGGCAG CCTCGCTCTG CCCACTGGGT GGGGCGGGAG GTAGGTGGGN
      481 N
      //
      Primer: T3
      Region: bGHpA,Diphtheria toxin A 3'
      Sequence file: RDB13081_A5Ajn.seq check
      >13081_13081_A5Aj_T3_8_C01_07.ab1
          1 ATGTCGGGAC CCCCTCGAGC CCCAGCTGGT TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC
         61 CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC
        121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA
        181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA
        241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGAG CTCTAGGATC
        301 TGCATTCCAC CACTGCTCCC ATTCATCAGT TCCATAGGTT GGAATCTAAA ATACACAAAC
        361 AATTAGAATC AGTAGTTTAA CACATTATAC ACTTAAAAAT TTTATATTTA CCTTAGAGCT
        421 TTAAATCTCT GTAGGTAGTT TGTCCAATTA TGTCACACCA CAGAAGTAAG GTTCCTTCAC
        481 AAAGAGATCG CCTGACACGA TTTCCTGCAC AGGCTTGAGC CATATACTCA TACATCGCAT
        541 CTTGGCCACG TTTTCCACGG GTTTCAAAAT TAATCTCAAG TTCTACGCTT AACGCTTTCG
        601 CCTGTTCCCA GTTATTAATA TATTCAACGC TAGAACTCCC CTCAGCGAAG GGAAGGCTGA
        661 GCACTACACG CGAAGCACCA TCACCGAACC TTTTGATAAA CTCTTCCGTT CCGACTTGCT
        721 CCATCAACGG TTCAGTGAGA CTTAAACCTA ACTCTTTCTT AATAGTTTCG GCATTATCCA
        781 CTTTTAGTGC GAGAACCTTC GTCAGTCCTG GATACGTCAC TTTGACCACG CCTCCAGCTT
        841 TTCCAGAGAG CGGGTTTTCA TTATCTACAG AGTATCCCGC AGCGTCGTAT TTATTGTCGG
        901 TACTATAAAA CCCTTTCCAA TCATCGTCAT AATTTCCTTG TGTACCAGAT TTTGGCTTTT
        961 GTATACCTTT TTTGAATGGA AATCTACATA ACCAGGGTTT AGTCCCGTGG TACGAAGAAA
       1021 AGTTTTCCAT CACAAAAGAT TTAGAGATCA CACATCATCA GGATCATGCG AGACTGCAGT
       1081 CGAAGGACAG AGATGAGGAA GAGAGAACAG CGCGCCAGGA CGTGCGCCTT TTTGAAGCGC
      1141 GGGTGCA
      //
      Primer: CMV_en_R
      Region: ROSA_3 3'
      Sequence file: RDB13081_A5Ajo.seq check
      >13081_13081_A5Aj_CMV_en_R_8_A10_01.ab1
          1 GTCTTTATTG ATTACTATTA ATAACTAGTC AATAATCAAT GTCGCACTTA ATTAAACGCG
         61 CCGGCTAGAA GATGGGCGGG AGTCTTCTGG GCAGGCTTAA AGGCTAACCT GGTGTGTGGG
        121 CGTTGTCCTG CAGGGGAATT GAACAGGTGT AAAATTGGAG GGACAAGACT TCCCACAGAT
        181 TTTCGGTTTT GTCGGGAAGT TTTTTAATAG GGGCAAATAA GGAAAATGGG AGGATAGGTA
        241 GTCATCTGGG GTTTTATGCA GCAAAACTAC AGGTTATTAT TGCTTGTGAT CCGCCTCGGA
        301 GTATTTTCCA TCGAGGTAGA TTAAAGACAT GCTCACCCGA GTTTTATACT CTCCTGCTTG
        361 AGATCCTTAC TACAGTATGA AATTACAGTG TCGCGAGTTA GACTATGTAA GCAGAATTTT
        421 AATCATTTTT AAAGAGCCCA GTACTTCATA TCCATTTCTC CCGCTCCTTC TGCAGCCTTA
        481 TCAAAAGGTA TTTTAGAACA CTCATTTTAG CCCCATTTTC ATTTATTATA CTGGCTTATC
        541 CAACCCCTAG ACAGAGCATT GGCATTTTCC CTTTCCTGAT CTTAGAAGTC TGATGACTCA
        601 TGAAACCAGA CAGATTAGTT ACATACACCA CAAATCGAGG CTGTAGCTGG GGCCTCAACA
        661 CTGCAGTTCT TTTATAACTC CTTAGTACAC TTTTTGTTGA TCCTTTGCCT TGATCCTTAA
        721 TTTTCAGTGT CTATCACCTC TCCCGTCAGG TGGTGTTCCA CATTTGGGCC TATTCTCAGT
        781 CCAGGGAGTT TTACAACAAT AGATGTATTG AGAATCCAAC CTAAAGCTTA ACTTTCCACT
        841 CCCATGAATG CCTCTCTCCT TTTTCTCCAT TTATAAACTG AGCTATTAAC CATTAATGGT
        901 TTCCAGGTGG ATGTCTCCTC CCCCAATATT ACCTGATGTA TCTTACATAT TGCCAGGCTG
        961 ATATTTTAAA GACATTAAAA GGTATATTTC ATTATTGAGC CACATGGGTA TTGATTACTG
       1021 CTTTACTAAA ATTTTGTCAT TGTACACATC TGTAAAAGGT GGTTTCCCTT TTGGGAATGC
       1081 AAAGTTCAGG TGATTGATGT CTTTCTGACC TTACGTCTGT GAGCCTGCAT TTTTTTCTAA
      1141 TTTAAGCAGG GTGCTTTTCT T
      //
      Primer: EGFP-C
      Region: mVenus-hGeminin1_110 3',bGHpA,ROSA_5
      Sequence file: RDB13081_A5Ajp.seq check
      >13081_13081_A5Aj_EGFP_C_8_D01_10.ab1
          1 GCGTACTCTC GGCATGGACG AGCTGTACAA GGGATATCCA TCACACTGGC GGCCGCTCGA
         61 GATGAATCCC AGTATGAAGC AGAAACAAGA AGAAATCAAA GAGAATATAA AGAATAGTTC
        121 TGTCCCAAGA AGAACTCTGA AGATGATTCA GCCTTCTGCA TCTGGATCTC TTGTTGGAAG
        181 AGAAAATGAG CTGTCCGCAG GCTTGTCCAA AAGGAAACAT CGGAATGACC ACTTAACATC
        241 TACAACTTCC AGCCCTGGGG TTATTGTCCC AGAATCTAGT GAAAATAAAA ATCTTGGAGG
        301 AGTCACCCAG GAGTCATTTG ATCTTATGAT TAAAGAAAAT CCATCCTCTC AGTATTGGAA
        361 GGAAGTGGCA GAAAAACGGA GAAAGGCGCT GTAAGGTACC ACATGTGGCG CGCGCGCCTT
        421 CGATGATCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
        481 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
        541 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
        601 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT
        661 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCTGCA GCCCAAGCTT GGGCTGCAGG
        721 GCGCGCCCGA TTAAGGCTAG AAAGACTGGA GTTGCAGATC ACGAGGGAAG AGGGGGAAGG
        781 GATTCTCCCA GGCCCAGGGC GGTCCTCAGA AGCCAGGAGG CAGCAGAGAA CTCCCAGAAA
        841 GGTATTGCAA CACTCCCCTC CCCCCTCCGG AGAAGGGTGC GGCCTTCTCC CCGCCTACTC
        901 CACTGCAGCT CCCTTACTGA TAACAACTCA GAGCGACTTT GGGAGAGCAA GTGCTTCCTG
        961 CCTCCAAAAC AGCCCAACTG AGCCCTCGCT CCTTTCCTCC ACTCCCCGGA GTGCGCGATG
       1021 GGAGGTCTGG CTCAGCACGC CCCTCTTGAG GCAACTCAAG TCGGGAAACG TGCTGCACCC
       1081 GCCCCGCAGC GCTCAGCCTT ACTGACGTTC CGCCCCAGCG CGCGCATCTG ACCACGTTGC
      1141 CGCCAAGGGG GACGTC
      //
      Primer: EGFP-N
      Region: mVenus-hGeminin1_110 5',T2A,mCherry-hCdt1_30_120
      Sequence file: RDB13081_A5Ajq.seq check
      >13081_13081_A5Aj_EGFP_N_8_A01_01.ab1
          1 GGCCCGGCGA CGCTCCTCGC CCTTGCTCAC CATGGGTCCA GGATTCTCTT CGACATCGCC
         61 ACAAGTGAGA AGAGAGCCAC GGCCTTCCAA TTGGATGGTG TCCTGGTCCT GCGCGGATGT
        121 CAGGTGGGGC GGCTGACCTG CTGCCGGGGT GGATTTCTTT ATCTTCTGGC CCGGAGAAGG
        181 GCAGGCTGGG ATGTCTGGGG CCTCGGGGGT ACTGGGGCTG GAAACCTCGT CCACCGACAG
        241 CCGCAGTCTC CTGCGGGCCG GTGGCCTGGC CTGGTCGCGT CCGGGGGCGG CGGGCGGGCG
        301 GGCGCGCTTG CGGCTGCCAC TGGTAGCGGA GGCCGGGGCG CGGAGTGCGG GCCTGGCGGG
        361 GCTGGGCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG TACAGCTCGT CCATGCCGCC
        421 GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG
        481 GGAGGTGATG TCCAACTTGA TGTTGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT
        541 GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT
        601 CTGCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC
        661 CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG
        721 CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA CGGTCACCAC
        781 GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA AGGACAGCTT
        841 CAAGTAGTCG GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
        901 GAGGGGACAG GATGTCCCAG GCGAAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG
        961 GCGGTCTGGG TGCCCTCGTA GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
       1021 CGTTCACGGG AGCCCTCCCA TGTGCACCTT TGAGCGCATG AACTCATGAA TGATGGCATG
       1081 ATATCTCCTC GCCTGCTCAC ATACGCGTGT CGAGAGTTCC CTGCCAGCCC GGGGGGAATC
      1141 CAA
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.
      reference Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
      reference Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
      reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
      reference Glover, J.D., Maintenance of distinct melanocyte populations in the interfollicular epidermis. Pigment Cell Melanoma Res. 28 (4): 476-480 (2015). PMID 25847135.

      2022.02.09

      GNP_filter3_RDBDEP_html_220112.pl