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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Fucci2a

Fucci2a cell cycle reporter.

Catalog number RDB13080
Resource name pCAG-Fucci2a
Clone info. Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120).
Vector backbone pCAGiP (Plasmid)
Selectable markers Ampicillin
Gene/insert name Human Cdt1 cDNA Human Gem cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Mort, Richard |
 
Other clones in our bank human CDT1 (NCBI Gene 81620) | human GMNN (NCBI Gene 51053) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
(3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
(4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
Ordering Please visit Information of Request for Distribution.[link] Please contact us. UCSD's MTA is also required.
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RDB13080 pCAG-Fucci2a DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0055e (English text)
Featured content Fucci-related materials (en) (English text)
Featured content Fucci-related materials (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13080_A5Ajp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: insert5' (mCherry-hCdt1(30_120))
Sequence file: RDB13080_A5Ajf.seq check
>13080_13080_A5Aj_pAxCA_F1_1_B04_04.ab1
    1 TCAAATTGCA CGAGTACCAC TGCTCAGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
   61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GACACGCGTA
  121 TGGTGAGCAA GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG
  181 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC
  241 GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT
  301 TCGCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC
  361 CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG
  421 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG
  481 GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA
  541 TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG
  601 CCCTGAAGGG CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG
  661 AGGTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA
  721 ACATCAAGTT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC
  781 GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC
  841 ACTGGCGGCC GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA
  901 CCAGTGGCAG CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CAGGGCCAGG
  961 GCCACCGGGC CCGCAGAGAC TGCGGGCTGT CGGTGGGACG AGGTTTCCAG CCCCCAGTAC
 1021 CCCCGAGCCC CAGACATCCC AGCCTGCCCT TCTCTGGACA GGAGATTAAG AAATCACTCA
1081 GGCAGCAGGT TCAGCCGACT CCAACCTTGA CATCCGTCGT CAGGAACA
//
Primer: BGH_rev
Region: insert3' (mVenus-hGeminin(1_110))
Sequence file: RDB13080_A5Ajg.seq check
>13080_13080_A5Aj_BGH_rev_1_D04_10.ab1
    1 GCAAATATCG AGCGCGCCAC ATGTGGTACC TTACAGCGCC TTTCTCCGTT TTTCTGCCAC
   61 TTCCTTCCAA TACTGAGAGG ATGGATTTTC TTTAATCATA AGATCAAATG ACTCCTGGGT
  121 GACTCCTCCA AGATTTTTAT TTTCACTAGA TTCTGGGACA ATAACCCCAG GGCTGGAAGT
  181 TGTAGATGTT AAGTGGTCAT TCCGATGTTT CCTTTTGGAC AAGCCTGCGG ACAGCTCATT
  241 TTCTCTTCCA ACAAGAGATC CAGATGCAGA AGGCTGAATC ATCTTCAGAG TTCTTCTTGG
  301 GACAGAACTA TTCTTTATAT TCTCTTTGAT TTCTTCTTGT TTCTGCTTCA TACTGGGATT
  361 CATCTCGAGC GGCCGCCAGT GTGATGGATA TCCCTTGTAC AGCTCGTCCA TGCCGAGAGT
  421 GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC
  481 TTTGCTCAGC TTGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC
  541 GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT
  601 GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC
  661 GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA
  721 GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC
  781 GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC
  841 GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA
  901 CTGCAGGCCG TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCCGG
  961 TGGTGCAGAT CAGCTTCAGG GTCAGCTTGC CCGTAGTGGC ATCGCCCCTC GCCCCTCGCC
 1021 GGACACGCTG AACCTTGTGG TCGTTTTACG TCGTCGGTCA GCTCGACAAG CATGGCCACC
1081 AGCTCGATGA CAGCCTTCCT TCGACCCATT GCTCACCCAT GGGGAT
//
Primer: Cdt1_F1
Region: insert mid (T2A,mVenus-hGeminin(1_110))
Sequence file: RDB13080_A5Ajh.seq check
>13080_13080_A9LhF_m_Cdt1_F1_D10_10_ABI24.ab1
    1 NNNNNNNNNN CTNNNNNNNN NNNNNNNNNN NNANNCCNGG ACCCATGGTG AGCAAGGGCG
   61 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  121 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
  181 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
  241 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
  301 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
  361 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
  421 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
  481 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT
  541 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
  601 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
  661 CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
  721 NCGCCGCCGG GATCACTCTC GGCATGGACG ANCTGTACAA GGGATATCCA TCACACTGGC
  781 GGCCGCTCGN GATGAATCCC AGTATGAAGC AGAAACNAGA AGAAATCAAA GAGAATATAA
  841 AGAATAGTTC TGTCCCNAGA ANAANTCTGA AGANGATTCN NCCNNNNNCA TCNNGNTCTC
  901 TNGTTGGAAG ANAAAATGAN CTNNCCNCNN NNNGNNCNAA NNNAANNNNN NGNNNNANNN
961 NTTNNNNTCN NNNNNTNNNN NNCNNNGGNN TNNNNNNNNN
//
Primer: EGFP-N
Region: insert mid (mVenus-hGeminin(1_110),T2A,mCherry-hCdt1(30_120))
Sequence file: RDB13080_A5Aji.seq check
>13080_13080_A5Aj_EGFP_N_1_C04_07.ab1
    1 GTACCGGATA CGCTCTCGCC CTTGCTCACC ATGGGTCCAG GATTCTCTTC GACATCGCCA
   61 CAAGTGAGAA GAGAGCCACG GCCTTCCAAT TGGATGGTGT CCTGGTCCTG CGCGGATGTC
  121 AGGTGGGGCG GCTGACCTGC TGCCGGGGTG GATTTCTTTA TCTTCTGGCC CGGAGAAGGG
  181 CAGGCTGGGA TGTCTGGGGC CTCGGGGGTA CTGGGGCTGG AAACCTCGTC CACCGACAGC
  241 CGCAGTCTCC TGCGGGCCGG TGGCCTGGCC TGGTCGCGTC CGGGGGCGGC GGGCGGGCGG
  301 GCGCGCTTGC GGCTGCCACT GGTAGCGGAG GCCGGGGCGC GGAGTGCGGG CCTGGCGGGG
  361 CTGGGCTCGA GCGGCCGCCA GTGTGATGGA TATCCCTTGT ACAGCTCGTC CATGCCGCCG
  421 GTGGAGTGGC GGCCCTCGGC GCGTTCGTAC TGTTCCACGA TGGTGTAGTC CTCGTTGTGG
  481 GAGGTGATGT CCAACTTGAT GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG
  541 GCCTTGTAGG TGGTCTTGAC CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC
  601 TGCTTGATCT CGCCCTTCAG GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC
  661 CAGCCCATGG TCTTCTTCTG CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC
  721 TTCACCTTGT AGATGAACTC GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG
  781 CCGCCGTCCT CGAAGTTCAT CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTT
  841 CAAGTAGTCG GGATGTCGGC GGGTGCTTCA CGTAGCCTTG GAGCCGTACA TGAACTGAGG
  901 GACAGATGTC CCAGCGAAGG CCAGGGGCAC CCTGTCACTC AGCTGGCCGT CTGGTTGCCT
  961 CGTAGGCCGT CTCGTCTCGC TCGATCTCGA CTCGTTGCCG TTCACGATCC TCATGGCACT
1021 GAGGCATGAG CTGAGATGCA GTTTAGCTCT GTCTTGCTAC A
//
Primer: CMV_en_R
Region: CMV en,SV40p(A),AmpR
Sequence file: RDB13080_A5Ajj.seq check
>13080_13080_A5Aj_CMV_en_R_1_A04_01.ab1
    1 GAGGCTATGA TTACTATTAT AACTAGTCAA TAATCAATGT CGACGATGAT ATCAGATCCA
   61 GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA
  121 TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT
  181 AAACAAGTTA ATTCGATTCA GCTTCAGAAG ATGGCGGAGG GCCTCCAACA CAGTAATTTT
  241 CCTCCCGACT CTTAAAATAG AAAATGTCAA GTCAGTTAAG CAGGAAGTGA CTAACTGACC
  301 GCAGCTGGCC GTGCGACATC CTCTTTTAAT TAGTTGCTAG GCAACTGCCC TCCAGAGGGC
  361 AGTGTGGTTT TGCAAGAGGA AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTCAGTGTG
  421 GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTTTC CACCCAATCA
  481 TTACTATGAC AACAGCTGTT TTTTTTAGTA TTAAGCAGAG GCCGGGGGCC CCTGGCCTCC
  541 GCTTACTCTG GAGAAAAAGA AGAGAGGCTT CCAGAGGCAA CTTGTCAAAA CAGGACTGGC
  601 GCCTTGGAGG CGCTGTGGGG CCACCCAAAT TGATATAATT AAGCCCCAAC CGCCTCTTCC
  661 CGCCTCATTT CAGCCTCACC ACTATGTGAA TATAGCTGAA TACACAGTTT ATTGAATAAA
  721 CATTAATTTC CAGGAAATAC AGTCTTTGTT TTTCCAAAGC GGTCATTGAA AAAGGAAGAG
  781 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGCATT TTGCCTTCCT
  841 GTTTTGCTCC ACCCAGAAAC GCTGGTGAAG TAAAAGATGC TGAGATCAGT GGGTGCACGA
  901 GTGGGTTACA TCGGACTGGA TCTCACAGCG TTAGGATTCT GGAGAGTTTC CGCCTCGAGA
  961 CCGTTGCATG ATGAGCACTT GAGGCTTCTG CTATGTGGGC CGATATCCGA TTGACCTGCA
1021 GGACTCTGTC GCCGATACGA TTCGATGACT GTTGATTCCA GTGCCGAAGG CT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.
reference Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
reference Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl