Fucci2a cell cycle reporter.
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested. (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested. (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
備考 | 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13080 | pCAG-Fucci2a | DNA solution |
Materials & Methods section:
The pCAG-Fucci2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13080). |
Reference section:
Mort, R.L., Ford, M.J., Sakaue-Sawano, A., Lindstrom, N.O., Casadio, A., Douglas, A.T., Keighren, M.A., Hohenstein, P., Miyawaki, A., Jackson, I.J., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Fucci by single plasmid (English text) |
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Featured content | Fucci-related materials (en) (English text) |
Featured content | Fucci-related materials (ja) (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13080_A5Ajp1-4.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCA_F1 (Pr0164) Region: insert 5' (mCherry-hCdt1(30_120) 5') Sequence file: RDB13080_A5Ajk.seq |
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>13080_13080_A5Aj_pAxCA_F1_1_B04_04.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCTNNTN NTTTTNCNNN CNNCTNNNNN 61 NCAACGNGCT GGTTATTGTG CTGNNNNNNN NTTTTGGCAA AGAATTCCTC GACACGCGTA 121 TGGTGAGCAA GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG 181 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC 241 GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT 301 TCGCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC 361 CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG 421 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG 481 GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA 541 TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG 601 CCCTGAAGGG CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG 661 AGGTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA 721 ACATCAAGTT GGACATCACC TCCCACAACG AGGANTACAC CATCGTGGAA CAGTACGAAC 781 GCGCCNANGG CCGCCACTCC NCCGGCGGCN TGGANGANCT GTACAANGGA NATCCNTCAC 841 ACTGGNGGNC NCTCNAGCCN NGCCCCGCNN GGNCNNNNCT NNNCGNCCNN GNCTNNGCTA 901 NNANNGGNAG NNNCAAGCGC GCCCGCCCGN NNGNNNNNNN NNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA,insert 3' (mVenus-hGeminin(1_110) 3') Sequence file: RDB13080_A5Ajl.seq |
>13080_13080_A9LhF_bGH_rev3_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNNGGGC ANACAACNGA TGGCTGGCAA CTAGAAGGCA 61 CAGTCGAGGC TGATCAGCGA GCTCTAGATC ATCGAAGGCG CGCCACATGT GGTACCTTAC 121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA 181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT 241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT 301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC 361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT 421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCC 481 TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC 541 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGTAGCT CAGGTAGTGG 601 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG 661 ANCTGCACGC CGCCGTCCTC NATGNTGNGG CGGANCTTGN ANTTGGCCNT GNTGNNNNNN 721 NNNGCNNNNN NNNNN // |
Primer: EGFP-N_R2 (Pr0905) Region: insert mid (mVenus-hGeminin(1_110) 5',T2A,mCherry-hCdt1(30_120) 3') Sequence file: RDB13080_A5Ajm.seq |
>13080_13080_A9LhF_EGFP-N_R2_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNNCNNNNCA NGCCGNANCC CANGGNGGTC 61 ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC 121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG 181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC 241 ACCATGGGTC CAGGATTCTC TTCGACATCG CCACAAGTGA GAAGAGAGCC ACGGCCTTCC 301 AATTGGATGG TGTCCTGGTC CTGCGCGGAT GTCAGGTGGG GCGGCTGACC TGCTGCCGGG 361 GTGGATTTCT TTATCTTCTG GCCCGGAGAA GGGCAGGCTG GGATGTCTGG GGCCTCGGGG 421 GTACTGGGGC TGGAAACCTC GTCCACCGAC AGCCGCAGTC TCCTGCGGGC CGGTGGCCTG 481 GCCTGGTCGC GTCCGGGGGC GGCGGGCGGG CGGGCGCGCT TGCGGCTGCC ACTGGTAGCG 541 GAGGCCGGGG CGCGGAGTGC GGGCCTGGCG GGGCTGGGCT CGAGCGGCCG CCAGTGTGAT 601 GGATATCCCT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG 661 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG 721 TTGTANGCGC CGGGCAGCTG CNCGGGCTTC TTGGCCTTGT ANGTGGTCTT GACCTCAGCG 781 NCGTANTGNN CGCCGTCCTT NNNCTTCAGC CTCTGCTTGA TCTCGCCCTT CNNNCNNCGN 841 CCTCGGGGNA NNNNCGCTCG GAGGANGNCN NCCCANNCCC ATGGTCTNNN TCTGNNTNAC 901 GGGGCCGTCN GAAGGGGAAN NTTGNNNGNC GCGCANCNTN CNNCNTTGNA NNATGANNTC 961 NNNNNNNNGN NGGNANGGAN TCCTGGNGNC NNGGTNNNCA NNNCGCNNNC NNNNAANTNN 1021 ANNNNNNGCT NCNNNNNNNN NN // |
Primer: Cdt1_F1 (Pr0768) Region: insert mid (T2A,mVenus-hGeminin(1_110) 5') Sequence file: RDB13080_A5Ajn.seq |
>13080_13080_A9LhF_m_Cdt1_F1_D10_10_ABI24.ab1 1 NNNNNNNNNN CTNNNNNNNN NNNNNNNNNN NNANNCCNGG ACCCATGGTG AGCAAGGGCG 61 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 121 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 181 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG 241 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 301 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA 361 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC 421 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT 481 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT 541 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA 601 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT 661 CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA 721 NCGCCGCCGG GATCACTCTC GGCATGGACG ANCTGTACAA GGGATATCCA TCACACTGGC 781 GGCCGCTCGN GATGAATCCC AGTATGAAGC AGAAACNAGA AGAAATCAAA GAGAATATAA 841 AGAATAGTTC TGTCCCNAGA ANAANTCTGA AGANGATTCN NCCNNNNNCA TCNNGNTCTC 901 TNGTTGGAAG ANAAAATGAN CTNNCCNCNN NNNGNNCNAA NNNAANNNNN NGNNNNANNN 961 NTTNNNNTCN NNNNNTNNNN NNCNNNGGNN TNNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP] |
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reference | Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140. [link to RRC of NBRP] |
reference | Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308. |
reference | Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130. |
user_report | Murganti, F., FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes. Front. Cardiovasc. Med. 9: 840147 (2022). PMID 35548410. [link to RRC of NBRP] |
user_report | Ducloux, C., Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status. Viruses 12 (12): 1467 (2020). PMID 33353185. [link to RRC of NBRP] |
2024.03.26
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