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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Fucci2a

Fucci2a cell cycle reporter.

Catalog number RDB13080
Resource name pCAG-Fucci2a
Clone info. Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120).
Vector backbone pCAGiP (Plasmid)
Selectable markers Ampicillin
Gene/insert name Human Cdt1 cDNA Human Gem cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Mort, Richard |
Other clones in our bank

External Database
Human Cdt1 Human Gem Discosoma sp. RFP
    Aequorea victoria GFP

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
        2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
        (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
        (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        Additional terms and conditions:
        RECIPIENT is requested to obtain written consent of Dr. Miyawaki, Team of the RIKEN CBS regarding Venus.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Approval form [Word] by Dr. Atsushi Miyawaki
        Remarks
        This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        Please obtain written approval of Dr. Atsushi Miyawaki.
        提供案内 (日本国内) [open/close]

        提供条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
        2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
        (3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
        (4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
        付加的提供条件:
        利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        宮脇敦史博士による提供承諾書 [Word]
        備考 提供条件は英文のまま記載してください。
        Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
        宮脇敦史博士の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB13080 pCAG-Fucci2a DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Original reference

        original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0055e (English text)
        Featured content Fucci-related materials (en) (English text)
        Featured content Fucci-related materials (ja) (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB13080_A5Ajp1-4.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pAxCA_F1
        Region: insert 5' (mCherry-hCdt1(30_120) 5')
        Sequence file: RDB13080_A5Ajk.seq check
        >13080_13080_A5Aj_pAxCA_F1_1_B04_04.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCCTNNTN NTTTTNCNNN CNNCTNNNNN
           61 NCAACGNGCT GGTTATTGTG CTGNNNNNNN NTTTTGGCAA AGAATTCCTC GACACGCGTA
          121 TGGTGAGCAA GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG
          181 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC
          241 GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT
          301 TCGCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC
          361 CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG
          421 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG
          481 GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA
          541 TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG
          601 CCCTGAAGGG CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG
          661 AGGTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA
          721 ACATCAAGTT GGACATCACC TCCCACAACG AGGANTACAC CATCGTGGAA CAGTACGAAC
          781 GCGCCNANGG CCGCCACTCC NCCGGCGGCN TGGANGANCT GTACAANGGA NATCCNTCAC
          841 ACTGGNGGNC NCTCNAGCCN NGCCCCGCNN GGNCNNNNCT NNNCGNCCNN GNCTNNGCTA
        901 NNANNGGNAG NNNCAAGCGC GCCCGCCCGN NNGNNNNNNN NNN
        //
        Primer: bGH_rev3
        Region: bGH pA,insert 3' (mVenus-hGeminin(1_110) 3')
        Sequence file: RDB13080_A5Ajl.seq check
        >13080_13080_A9LhF_bGH_rev3_B01_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNGNNGGGC ANACAACNGA TGGCTGGCAA CTAGAAGGCA
           61 CAGTCGAGGC TGATCAGCGA GCTCTAGATC ATCGAAGGCG CGCCACATGT GGTACCTTAC
          121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA
          181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT
          241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT
          301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC
          361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT
          421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCC
          481 TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC
          541 ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGTAGCT CAGGTAGTGG
          601 TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG
          661 ANCTGCACGC CGCCGTCCTC NATGNTGNGG CGGANCTTGN ANTTGGCCNT GNTGNNNNNN
        721 NNNGCNNNNN NNNNN
        //
        Primer: EGFP-N_R2
        Region: insert mid (mVenus-hGeminin(1_110) 5',T2A,mCherry-hCdt1(30_120) 3')
        Sequence file: RDB13080_A5Ajm.seq check
        >13080_13080_A9LhF_EGFP-N_R2_A03_03_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNNCNNNNCA NGCCGNANCC CANGGNGGTC
           61 ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATCAGCTT CAGGGTCAGC
          121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG
          181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC
          241 ACCATGGGTC CAGGATTCTC TTCGACATCG CCACAAGTGA GAAGAGAGCC ACGGCCTTCC
          301 AATTGGATGG TGTCCTGGTC CTGCGCGGAT GTCAGGTGGG GCGGCTGACC TGCTGCCGGG
          361 GTGGATTTCT TTATCTTCTG GCCCGGAGAA GGGCAGGCTG GGATGTCTGG GGCCTCGGGG
          421 GTACTGGGGC TGGAAACCTC GTCCACCGAC AGCCGCAGTC TCCTGCGGGC CGGTGGCCTG
          481 GCCTGGTCGC GTCCGGGGGC GGCGGGCGGG CGGGCGCGCT TGCGGCTGCC ACTGGTAGCG
          541 GAGGCCGGGG CGCGGAGTGC GGGCCTGGCG GGGCTGGGCT CGAGCGGCCG CCAGTGTGAT
          601 GGATATCCCT TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG
          661 TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG
          721 TTGTANGCGC CGGGCAGCTG CNCGGGCTTC TTGGCCTTGT ANGTGGTCTT GACCTCAGCG
          781 NCGTANTGNN CGCCGTCCTT NNNCTTCAGC CTCTGCTTGA TCTCGCCCTT CNNNCNNCGN
          841 CCTCGGGGNA NNNNCGCTCG GAGGANGNCN NCCCANNCCC ATGGTCTNNN TCTGNNTNAC
          901 GGGGCCGTCN GAAGGGGAAN NTTGNNNGNC GCGCANCNTN CNNCNTTGNA NNATGANNTC
          961 NNNNNNNNGN NGGNANGGAN TCCTGGNGNC NNGGTNNNCA NNNCGCNNNC NNNNAANTNN
        1021 ANNNNNNGCT NCNNNNNNNN NN
        //
        Primer: Cdt1_F1
        Region: insert mid (T2A,mVenus-hGeminin(1_110) 5')
        Sequence file: RDB13080_A5Ajn.seq check
        >13080_13080_A9LhF_m_Cdt1_F1_D10_10_ABI24.ab1
            1 NNNNNNNNNN CTNNNNNNNN NNNNNNNNNN NNANNCCNGG ACCCATGGTG AGCAAGGGCG
           61 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
          121 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
          181 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG
          241 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
          301 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
          361 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
          421 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
          481 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT
          541 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA
          601 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT
          661 CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
          721 NCGCCGCCGG GATCACTCTC GGCATGGACG ANCTGTACAA GGGATATCCA TCACACTGGC
          781 GGCCGCTCGN GATGAATCCC AGTATGAAGC AGAAACNAGA AGAAATCAAA GAGAATATAA
          841 AGAATAGTTC TGTCCCNAGA ANAANTCTGA AGANGATTCN NCCNNNNNCA TCNNGNTCTC
          901 TNGTTGGAAG ANAAAATGAN CTNNCCNCNN NNNGNNCNAA NNNAANNNNN NGNNNNANNN
        961 NTTNNNNTCN NNNNNTNNNN NNCNNNGGNN TNNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.
        reference Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140.
        reference Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
        reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
        user_report Ducloux, C., Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status. Viruses 12 (12): 1467 (2020). PMID 33353185.

        2022.10.12

        GNP_filter3_RDBDEP_html_221010.pl