Resource data sheet
Prev.

CMV-V5-CTNNB1(WT)

Expression plasmid of human catenin beta 1, wild type. Gateway(R) expression clone.

Catalog number RDB13062
Resource name CMV-V5-CTNNB1(WT)
Alternative name CMV-b-catenin WT
Clone info. Expression plasmid of human catenin beta 1, wild type.
Gateway(R) expression clone. Insert fragment was derived from IOH29101.
Vector backbone pcDNA3.1/V5-DEST (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Human CTNNB1 cDNA
Depositor Gondo, Yoichi | Murata, Takuya |
 
Other clones in our bank human CTNNB1 (NCBI Gene 1499) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Murata T. et al., Sci. Rep. 4: 6959, 2014)
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Remarks An additional fee for license fee of Gateway(R) Expression Vector is required.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB13062 CMV-V5-CTNNB1(WT) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Murata, T., Beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia. Sci. Rep. 4: 6959 (2014). PMID 25376241.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16717_A8Isp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB16717_A8Isa.seq
>13062_16717_A8Is_CMV-Forward_C01_07_ABI24.ab1
    1 ATAAAAACCT CCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGTTAAGC TTACCATGGG TAAGCCTATC
  181 CCTAACCCTC TCCTCGGTCT CGATTCTACG GAAAACCTGT ATTTTCAGGG CCCGGATCCG
  241 TCGACGAATT CTGCAGATAT CACAAGTTTG TACAAAAAAG CAGGCACCAT GGCTACTCAA
  301 GCTGATTTGA TGGAGTTGGA CATGGCCATG GAACCAGACA GAAAAGCGGC TGTTAGTCAC
  361 TGGCAGCAAC AGTCTTACCT GGACTCTGGA ATCCATTCTG GTGCCACTAC CACAGCTCCT
  421 TCTCTGAGTG GTAAAGGCAA TCCTGAGGAA GAGGATGTGG ATACCTCCCA AGTCCTGTAT
  481 GAGTGGGAAC AGGGATTTTC TCAGTCCTTC ACTCAAGAAC AAGTAGCTGA TATTGATGGA
  541 CAGTATGCAA TGACTCGAGC TCAGAGGGTA CGAGCTGCTA TGTTCCCTGA GACATTAGAT
  601 GAGGGCATGC AGATCCCATC TACACAGTTT GATGCTGCTC ATCCCACTAA TGTCCAGCGT
  661 TTGGCTGAAC CATCACAGAT GCTGAAACAT GCAGTTGTAA ACTTGATTAA CTATCAAGAT
  721 GATGCAGAAC TTGCCACACG TGCAATCCCT GAACTGACAA AACTGCTAAA TGACGAGGAC
  781 CAGGTGGTGG TTAATAAGGC TGCAGTTATG GTCCATCAGC TTTCTAAAAA GGAAGCTTCC
  841 AGACACGCTA TCATGCGTTC TCCTCAGATG GTGTCTGCTA TTGTACGTAC CATGCAGAAT
  901 ACAAATGATG TAGAAACAGC TCGTTGTACC GCTGGGACCT TGCATAACCT TTCCCATCAT
  961 CGTGAGGGCT TACTGGCCAT CTTTAAGTCT GGAGGCATTC CTGCCCTGGG TGAAAATGCT
 1021 TGGTCACCAG TGGATTCTGT GTTGGTTTTT ATGGCCATTA CAACTCTCAA CAACCTTTTA
 1081 TTACATCAGA GAGCTAAAAA TGCAAGTGCC GTTAGCTTGT GGCTGCAGAA ATGCTGGCTG
1141 GCTCAAACAA AACAAATGTT TAAATTTCTC TTTGTC
//
Primer: BGH_rev2 RDB16717_A8Isb.seq
>13062_16717_A8Is_BGH_rev2_D01_10_ABI24.ab1
    1 TCGGGGGTTA AGGGGGAAGG CCACGGGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA
   61 GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TATCACCACT TTGTACAAGA AAGCTGGGTT
  121 CTACAGGTCA GTATCAAACC AGGCCAGCTG ATTGCTGTCA CCTGGAGGCA GCCCATCCAT
  181 GAGGTCCTGG GCATGCCCCA GATCTGGCAG CCCATCAACT GGATAGTCAG CACCAGGGTG
  241 GTGGCCACCC ATCTCATGTT CCATCATGGG GTCCATACCC AAGGCATCCT GGCCATATCC
  301 ACCAGAGTGA AAAGAACGAT AGCTAGGATC ATCCTGGCGA TATCCAAGGG GTTCTCCCTG
  361 GGCACCAATA TCAAGTCCAA GATCAGCAGT CTCATTCCAA GCCATTGGCT CTGTTCTGAA
  421 GAGAGAGCTG GTCAGCTCAA CTGAAAGCCG TTTCTTGTAA TCTTGTGGCT TGTCCTCAGA
  481 CATTCGGAAC AAAACAGCAG CTGCATATGT CGCCACACCT TCATTCCTAG AGTGAAGTAA
  541 CTCTGTCAGA GGAGCTGTGG CTCCCTCAGC TTCAATAGCT TCTGCAGCTT CCTTGTCCTG
  601 AGCAAGTTCA CAGAGGACCC CTGCAGCTAC TCTTTGGATG TTTTCAATGG GAGAATAAAG
  661 CAGCTGCACA AACAATGGAA TGGTATTTAG TCCTCTGATA ACAATTCGGT TGTGAACATC
  721 CCGAGCTAGG ATGTGAAGGG CTCCGGTACA ACCTTCAACT ATTTCTTCCA TGCGGACCCC
  781 CTCCACAAAT TGCTGCTGTG TCCCACCCAT GGACGTACGG CGCTGGGTAT CCTGATGTGC
  841 ACGAACAAGC AACTGAACTA GTCGTGGAAT GGCACCCTGC TCACGCAAAG GTGCATGATT
  901 TGCGGGACAA AGGGGCAAGA TTTCGAATCA ATCCAACAGT AGCCTTTATC AGAGGCCAGT
  961 GGGATGGTGG GGTGTAAGGA GCTTAACCAA CAAACTGGTA GTCCAATAGT TGAAAGGCGA
 1021 AACTGCATTC TGGGGGCCAT CTCTGCTTTC TTGGTTGTCG GGCCTGGTCA GAATGACGAA
 1081 GAGGCACAGA TGGGCAAGGC TCAGATGAAT GTCCTTCCCC TGGTCACCAG CCCGAAAGGA
 1141 ACAGGTACGG CACAAGAAGG CCCTTCTATT ATACAACCCA ACTTGTGGCA AGAAAACCAA
1201 TCATCTTTGG TTCTCTCTTA ATTAAGT
//
Primer: hCTNNB1-M_R RDB16717_A8Isc.seq
>13062_16717_A8Is_hCTNNB1-M_R_E01_13_ABI24.ab1
    1 GCAGAAGGAT AGGCACCTTC AGCACTCTGC TTGTGGTCCA CAGTAGTTTT TCGTAAGTAT
   61 AGGTCCTCAT TATATTTACT AAAGCTTGGG GTCCACCACT AGCCAGTATG ATGAGCTTGC
  121 TTTCTTGGTT GCCATAAGCT AAAATTTGAA GGCAGTCTGT CGTAATAGCC AAGAATTTAA
  181 CATTTGTTTT GTTGAGCAAG GCAACCATTT TCTGCAGCCC ACCAGCTAAA CGCACTGCCA
  241 TTTTAGCTCC TTCTTGATGT AATAAAAGGT TGTGGAGAGT TGTAATGGCA TAAAACAACA
  301 CAGAATCCAC TGGTGAACCA AGCATTTTCA CCAGGGCAGG AATGCCTCCA GACTTAAAGA
  361 TGGCCAGTAA GCCCTCACGA TGATGGGAAA GGTTATGCAA GGTCCCAGCG GTACAACGAG
  421 CTGTTTCTAC ATCATTTGTA TTCTGCATGG TACGTACAAT AGCAGACACC ATCTGAGGAG
  481 AACGCATGAT AGCGTGTCTG GAAGCTTCCT TTTTAGAAAG CTGATGGACC ATAACTGCAG
  541 CCTTATTAAC CACCACCTGG TCCTCGTCAT TTAGCAGTTT TGTCAGTTCA GGGATTGCAC
  601 GTGTGGCAAG TTCTGCATCA TCTTGATAGT TAATCAAGTT TACAACTGCA TGTTTCAGCA
  661 TCTGTGATGG TTCAGCCAAA CGCTGGACAT TAGTGGGATG AGCAGCATCA AACTGTGTAG
  721 ATGGGATCTG CATGCCCTCA TCTAATGTCT CAGGGAACAT AGCAGCTCGT ACCCTCTGAG
  781 CTCGAGTCAT TGCATACTGT CCATCAATAT CAGCTACTTG TTCTTGAGTG AAGGACTGAG
  841 AAAATCCCTG TTCCCACTCA TACAGGACTT GGGAGGTATC CACATCCTCT TCCTCAGGAT
  901 TGCCTTTACC ACTCAGAGAA GGAGCTGTGG TAGTGGCACC AGAAATGGAT TCCAGAGTCC
  961 AGGTAAGACT GTTTGCTGCC AGTGACTAAC AGCCCGCTTT TCTGTCTGGT TTCCATGGGC
 1021 CATGTCAACT CCATCAAATC AGCTTGAGTA GCCATGGTGC CTGCTTTTTT TGTACAAACC
 1081 TTGTGGATAT CCTGCCAGAT TCGGTCGAAC AGAATCGGCT TGAAATACAT GGTTTCCGTT
1141 AGAATCGAGA CCGTAGGGAG AGGGGGT
//
Primer: SV40pro_F RDB16717_A8Isd.seq
>13062_16717_A8Is_SV40pro_F_F01_16_ABI24.ab1
    1 CCTGGCCCCT GAACTTTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGGCTT
 1021 CGGAATCGGT TTTCCCGGGA CGCCGCTGGA TGATCCTCAG CGGCGGGGAT CTCATGCTGG
 1081 AGACCTCGCC CACCCAACTT GGTTTATTGG CAAGCTATAT GGTTACCGAT TAAGCCAATA
1141 GCCATCACAA AATTTTCACA CAAAATAAAG GCAAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Murata, T., Beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia. Sci. Rep. 4: 6959 (2014). PMID 25376241.

2018.10.18

GNP_filter3_RDBDEP_html_181012.pl