Resource data sheet
Prev.

pSPCiEF1>Cas9

Expression plasmid of Cas9

Catalog number RDB12999
Resource name pSPCiEF1>Cas9
Clone info. Expression plasmid of Cas9. The expression of Cas9 is under the control of Ciona EF1alpha promoter.
See Fig. 4A of Develop. Growth Differ. (2014) 56, 499-510.
Vector backbone pSP1.72 (Plasmid)
Size of vector backbone 2.5 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Ciona intestinalis EF1 alpha genomic DNA SV40 genomic DNA
Depositor Sasakura, Yasunori |
 
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Please use the Exclusive MTA (genome editing). [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12999 pSPCiEF1>Cas9 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

original Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl