Resource data sheet
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pSPCiEF1>Cas9

Expression plasmid of Cas9

Catalog number RDB12999
Resource name pSPCiEF1>Cas9
Clone info. Expression plasmid of Cas9. The expression of Cas9 is under the control of Ciona EF1alpha promoter.
See Fig. 4A of Develop. Growth Differ. (2014) 56, 499-510.
Vector backbone pSP1.72 (Plasmid)
Size of vector backbone 2.5 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Ciona intestinalis EF1 alpha genomic DNA SV40 genomic DNA
Depositor Sasakura, Yasunori |
 
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) | Ciona intestinalis |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
(Japanese text [open/close])
Remarks
Please use the Exclusive MTA (genome editing) [Word] [pdf]
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12999 pSPCiEF1>Cas9 DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

original Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715.

2017.10.19

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