Resource data sheet
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CSIV-TRE-mOct3/4-CMV-KT

Lentivirus vector construct of mouse Oct3/4 driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Catalog number RDB12961
Resource name CSIV-TRE-mOct3/4-CMV-KT
Clone info. Lentivirus vector construct of mouse Oct3/4 driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT (Plasmid)
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Mouse Oct3/Oct4 cDNA
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12961 CSIV-TRE-mOct3/4-CMV-KT DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12961z.seq from Depositor
Electronic file PDF (map, protocol and/or remarks) RDB12961.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12961_A4Kbp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F RDB12961_A4Kba.seq
>12961_12961_A4Kb_1_cPPT+CTS_F_A01_01_ABI24.ab1
    1 ATAGTTGATT CGGCCTTTCG TCTTCATCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA
   61 TGTCGAGTTT ACTCCCTATC AGTGATAGAG AACGATGTCG AGTTTACTCC CTATCAGTGA
  121 TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTACT
  181 CCCTATCAGT GATAGAGAAC GTATGTCGAG TTTATCCCTA TCAGTGATAG AGAACGTATG
  241 TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GGTAGGCGTG TACGGTGGGA
  301 GGCCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCGCTAGCA TCACAAGTTT
  361 GTACAAAAAA GCAGGCTCCG CGGCCGCCCC CTTCACCATG GCTGGACACC TGGCTTCAGA
  421 CTTCGCCTTC TCACCCCCAC CAGGTGGGGG TGATGGGTCA GCAGGGCTGG AGCCGGGCTG
  481 GGTGGATCCT CGAACCTGGC TAAGCTTCCA AGGGCCTCCA GGTGGGCCTG GAATCGGACC
  541 AGGCTCAGAG GTATTGGGGA TCTCCCCATG TCCGCCCGCA TACGAGTTCT GCGGAGGGAT
  601 GGCATACTGT GGACCTCAGG TTGGACTGGG CCTAGTCCCC CAAGTTGGCG TGGAGACTTT
  661 GCAGCCTGAG GGCCAGGCAG GAGCACGAGT GGAAAGCAAC TCAGAGGGAA CCTCCTCTGA
  721 GCCCTGTGCC GACCGCCCCA ATGCCGTGAA GTTGGAGAAG GTGGAACCAA CTCCCGAGGA
  781 GTCCCAGGAC ATGAAAGCCC TGCAGAAGGA GCTAGAACAG TTTGCCAAGC TGCTGAAGCA
  841 GAAGAGGATC ACCTTGGGGG TACACCCAGG CCGACGTGGG GCTCACCCTG GGCGTTCTCT
  901 TTGGAAAGGT GTTCAGCCAG ACCACCATCT GTCGCTTCGA GGCCTTGCAG CTCAGCCTTA
  961 AGAACATGTG TAAGCTGCGG CCCCTGCTGG GAGAAGTGGG TGGGAGGAAG CCCGACACAT
 1021 GAGAACCTTT CAGGAGATAT GCAATCGGAG ACCCTGGTGC AGCCCAGCAG AGAAGCGACT
 1081 AGCATTGAGA CCGTGTGAAG TGCAAGTCTG GAGACAATGC TTTCTGGAAG TGCCCCGAAG
1141 GCCCCATC
//
Primer: hKO_F RDB12961_A4Kbb.seq
>12961_12961_A4Kb_1_hKO_F_B01_04_ABI24.ab1
    1 CGTATCGGCA AGGCTGGTGA GGAGACCGAG GGCAACATCA CCGAGCTGGT GGAGGACGCC
   61 GTGGCCCACT GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAGAATCCC
  121 GGCCCTATGT CTAGACTGGA CAAGAGCAAA GTCATAAACG GCGCTCTGGA ATTACTCAAT
  181 GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC AAAAGCTGGG AGTTGAGCAG
  241 CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC TCGATGCCCT GCCAATCGAG
  301 ATGCTGGACA GGCATCATAC CCACTTCTGC CCCCTGGAAG GCGAGTCATG GCAAGACTTT
  361 CTGCGGAACA ACGCCAAGTC ATTCCGCTGT GCTCTCCTCT CACATCGCGA CGGGGCTAAA
  421 GTGCATCTCG GCACCCGCCC AACAGAGAAA CAGTACGAAA CCCTGGAAAA TCAGCTCGCG
  481 TTCCTGTGTC AGCAAGGCTT CTCCCTGGAG AACGCACTGT ACGCTCTGTC CGCCGTGGGC
  541 CACTTTACAC TGGGCTGCGT ATTGGAGGAA CAGGAGCATC AAGTAGCAAA AGAGGAAAGA
  601 GAGACACCTA CCACCGATTC TATGCCCCCA CTTCTGAGAC AAGCAATTGA GCTGTTCGAC
  661 CGGCAGGGAG CCGAACCTGC CTTCCTTTTC GGCCTGGAAC TAATCATATG TGGCCTGGAG
  721 AAACAGCTAA AGTGCGAAAG CGGCGGGCCG GCCGACGCCC TTGACGATTT TGACTTAGAC
  781 ATGCTCCCAG CCGATGCCCT TGACGACTTT GACCTTGATA TGCTGCCTGC TGACGCTCTT
  841 GACGATTTTG ACCTTGACAT GCTCCCCGGG TAACTAAGTA AGGATCCTCT AGAGTTAACA
  901 TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC
  961 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAAATGCCCT
 1021 TTGTATCATG CTATTGCTTT CCCGTATGGC TTTCATTTTC TCCTCGTGGT ATAAATTCTT
 1081 GGCTGGCTGG TCTCTTATGA GAGTGTGGCT CGATGTCAGC ACGTTGGCGT GGATTGCACT
1141 GGTATTTGAC CTTGAACGGT CCA
//
Primer: HIVgb_R1 RDB12961_A4Kbc.seq
>12961_12961_A4Kb_1_HIVgb_R1_C01_07_ABI24.ab1
    1 TAGTTTCCCA CTGCTCTTTT TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT
   61 ACTCCTAATG GTTCAATTTT TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC
  121 CTCATATCTC CTCCTCCAGG TCTGAAGATC AGCGGCCGCT TGCTGTGCGG TGGTCTTACT
  181 TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA TCTCTATCCT
  241 TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT CTTCTGATCC
  301 TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG ATGTTTCTAA
  361 CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA TATGTTTTAA
  421 TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG ATCTAATTCT
  481 CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC CGCTAGTCAA
  541 AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG CTTCAGCAAG
  601 CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT CCCTGTTCGG
  661 GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT CTAGTTACCA
  721 GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT TTATTGAGGC
  781 TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG GTCTAACCAG
  841 AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA CACGCCTACC
  901 GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG
  961 GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAATC CCCGTGAGTC
 1021 AAACCGCTAT CCACGCCCAT TGATGTACTG CAAAACGCAT CACCATGGGT AATAGCGGAT
 1081 GACTATACGT AGATGTACTG CCAGTAAGGA AGGTCCCATA GTCATGTACT GGGGCAATAA
1141 TGCCAGGCCG
//
Primer: BGH_rev2 RDB12961_A4Kbd.seq
>12961_12961_A4Kb_1_BGH_rev2_D01_10_ABI24.ab1
    1 CAATATCACA TAGAAGCACG GGGGAGGGGC AAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGAAT AACTTCGTAT
  301 AATGTATGCT ATACGAAGTT ATGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA
  361 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC
  421 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC
  481 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA
  541 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC
  601 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG
  661 CTGGATTGAG GGCCGAAGGG ACGTAGCAGA AGGACGTCCC GCGCAGAATC CAGGTGGCAA
  721 CACAGGCGAG CAGCCATGGA AAGGACGTCA GCTTCCCCGA CAACACCACG GAATTGTCAG
  781 TGCCCAACAG CCGAGCCCCT GTCCAGCAGC GGGCAAGGCA GGCGGCGATG AGTTCCGCCG
  841 TGGCAATAGG GAGGGGGAAA GCGAAAGTCC CGGAAAGGAG CTGACAGGTG GTGGCAATGC
  901 CCCAACCAGT GGGGGGTTGC GTCAGCAAAC ACAGTGCACA CCACGCCACG TTGCCTGACA
  961 ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGGAT TTATACAAGG AGGAGAAAAT
 1021 GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG
 1081 CGTAAAAGGA GCACATAGTT TAGATACCCA GGTCATCTTC ACAAATTTTG GTATTCAGAG
1141 GATGATTTAT TCGAATAAGC CTGGGAATTC CT
//
Primer: hKO_N_R RDB12961_A4Kbe.seq
>12961_12961_A4Kb_1_hKO_N_R_E01_13_ABI24.ab1
    1 CCAGTAGAAT TCATCTTCAT CTCGGGCTTG ATCACGCTCA CCATGGTGGA CCGGTGAATT
   61 CGGGGCCGCG GAGGCTGGAT CGGTCCCGGT GTCTTCTATG GAGGTCAAAA CAGCGTGGAT
  121 GGCGTCTCCA GGCGATCTGA CGGTTCACTA AACGAGCTCT GCTTATATAG ACCTCCCACC
  181 GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT
  241 CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA
  301 ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA
  361 TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA
  421 TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC
  481 TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC
  541 ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG
  601 TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA
  661 CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC
  721 TAGTCAATAA TCAATGTCAA CATGGCGGTA ATGTTGGACA TGAGCCAATA TAAATGTACA
  781 TATTATGATA TGGATACAAC GTATGCAATG GGCCAAGCTC CTCGAGACCG GTAGCATCAC
  841 CACTTTGTAC AAGAAAGCTG GGTCGGCGCG CCCACCCTTT CAGTTTGAAT GCATGGGAGA
  901 GCCCAGAGCA GTGACGGGAA CAGAGGGAAA GGCCTCGCCT CTCAGGAAAA GGGACTGAGT
  961 AGAGTGTGGT GAAGTGGGGG CTTTCTATAG CCCTGGGGGT GCCCAAAGTG GGGATCTGGG
 1021 GGGCAGAGGA AGGATACAGC CCCCCTCCTG CGAAAAGGTG TCCCTGTAGC CTTCATACTC
 1081 CTTCTCGGTG GGTATACCTC ATACTTGATC TTTGCCGTCC TGCGCGATAC AGACCCATAA
1141 CCTTCGAC
//
Primer: SV40pro_F RDB12961_A4Kbf.seq
>12961_12961_A4Kb_1_SV40pro_F_F01_16_ABI24.ab1
    1 GCCTGGTCCT GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
  961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCG GGCCAACGCG CGGGGAGAGG CGGGTTGCGT ATTGGGGCGC TCTTCGCTTC
 1081 TCGCTCACTG AACTCGCTGC GCTCGGTCGG TTCGGCTGCG GCGAGCCGCT ATCAGCTCAC
1141 GTCAAGGTCG GTTAAATACC GGGGT
//
Primer: Oct4_N_R RDB12961_A4Kbg.seq
>12961_12961_A4Kb_Oct4_N_R_C01_03_ABI08.ab1
    1 TCTCTTTTAG GGCGGGGGCC GGGAATGGGG GAGATCCCCA ATAACCTCTG AGCCTGGTCC
   61 GATTCCAGGC CCACCTGGAG GCCCTTGGAA GCTTAGCCAG GTTCGAGGAT CCACCCAGCC
  121 CGGCTCCAGC CCTGCTGACC CATCACCCCC ACCTGGTGGG GGTGAGAAGG CGAAGTCTGA
  181 AGCCAGGTGT CCAGCCATGG TGAAGGGGGC GGCCGCGGAG CCTGCTTTTT TGTACAAACT
  241 TGTGATGCTA GCGGCGATCT GACGGTTCAC TAAACGAGCT CTGCTTATAT AGGCCTCCCA
  301 CCGTACACGC CTACCTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC
  361 GTTCTCTATC ACTGATAGGG ATAAACTCGA CATACGTTCT CTATCACTGA TAGGGAGTAA
  421 ACTCGACATA CGTTCTCTAT CACTGATAGG GAGTAAACTC GACATACGTT CTCTATCACT
  481 GATAGGGAGT AAACTCGACA TCGTTCTCTA TCACTGATAG GGAGTAAACT CGACATACGT
  541 TCTCTATCAC TGATAGGGAG TAAACTCGAT TGAAGACGAA AGGGCCGAAT TCAAAGTGAA
  601 TTTCTGCTGT CCCTGTAATA AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT
  661 TCTTTAGTTT GTATGTCTGT TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC
  721 CCAATCCCCC CTTTTCTTTT AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC
  781 TAATCGAATG GATCTGTCTC TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT
  841 GTCGGGTCCC CTCGGGGTTG GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCC
  901 TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT TAAACCCTAC CAAAGCCTCC
  961 TACTATCATT TATGAAATAA ATTTTATATA CCACAGCCAA TTTGTTTATG GTAAACCCAA
 1021 TTCCCACCAA ACCTTGCCCA TTTAATCTAA TTTCCATTAA TTTCTGTTCA GTCCTTTTCC
1081 TTGGCTGGTT TGCGATTCTT CAATAAGGGG AGTGTGTATA TTTAAAAGG
//

Please visit Sequencing and PCR primers for primer information.


References

References


2019.04.18

GNP_filter3_RDBDEP_html_190418.pl