Resource data sheet
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CSIV-TRE-hc-Myc-CMV-KT

Lentivirus vector plasmid of human MYC driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Catalog number RDB12923
Resource name CSIV-TRE-hc-Myc-CMV-KT
Clone info. Lentivirus vector plasmid of human MYC driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSIV-TRE-RfA-CMV-KT (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human MYC cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank
External Database
Human MYC
          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
          Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding hKO1 fluorescent protein.
          Ordering Please visit Ordering instruction.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
          Please contact us. We will provide you with appropriate documents.
          Please obtain written approval of Dr. Atsushi Miyawaki.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
          付加的提供条件 利用者は、hKO1蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          宮脇敦史博士による提供承諾書 [Word]
          備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          宮脇敦史博士の書面による提供承諾が必要です。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB12923 CSIV-TRE-hc-Myc-CMV-KT Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          References and tips

          Electronic file

          Sequence RDB12923z.seq
          Remarks, protocol and/or map (pdf) RDB12923.pdf

          Original reference

          original Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346.

          2022.05.17

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