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CSIV-TRE-hc-Myc-CMV-KT

Lentivirus vector plasmid of human MYC driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Catalog number RDB12923
Resource name CSIV-TRE-hc-Myc-CMV-KT
Clone info. Lentivirus vector plasmid of human MYC driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-TRE-RfA-CMV-KT (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human MYC cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank
External Database
Human MYC
          Reference sequence
            
           
          Sequence RDB12923z.seq
          Remarks, protocol and/or map (pdf) RDB12923.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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          Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
          Please contact us. We will provide you with appropriate documents.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
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          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB12923 CSIV-TRE-hc-Myc-CMV-KT Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering instruction of plasmids [in Japanese] [in English]

          How to cite this biological resource

          Materials & Methods section:

          The CSIV-TRE-hc-Myc-CMV-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12923).

          Reference section:

          Tai, M.C., Kajino, T., Nakatochi, M., Arima, C., Shimada, Y., Suzuki, M., Miyoshi, H., Yatabe, Y., Yanagisawa, K., Takahashi, T., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content Lentiviral plasmid list (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346. [link to RRC of NBRP]

          2023.11.16

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