RDB12911 - CSII-EF-hc-Myc-IRES2-Venus

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CSII-EF-hc-Myc-IRES2-Venus (#RDB12911)

Lentivirus vector plasmid of human MYC for generating iPS cells.

Clone info.	Lentivirus vector plasmid of human MYC for generating iPS cells.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human MYC cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB12911z.seq
Remarks, protocol and/or map (pdf)	RDB12911.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12911	CSII-EF-hc-Myc-IRES2-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-hc-Myc-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12911).

Reference section:

Fujioka, T., Shimizu, N., Yoshino, K., Miyoshi, H., Nakamura, Y., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12911_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12911_A4Jfa.seq
>12911_12911_A4Jf_pAxEF_F2_1_F04_16.ab1 1 GATTTCTCTC CTTTTAGGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT CTGCAGCGGC 61 CATCACAAGT TTGTACAAAA AAGCAGGCTC CGCGGCCGCC CCCTTCACCA TGCCCCTCAA 121 CGTTAGCTTC ACCAACAGGA ACTATGACCT CGACTACGAC TCGGTGCAGC CGTATTTCTA 181 CTGCGACGAG GAGGAGAACT TCTACCAGCA GCAGCAGCAG AGCGAGCTGC AGCCCCCGGC 241 GCCCAGCGAG GATATCTGGA AGAAATTCGA GCTGCTGCCC ACCCCGCCCC TGTCCCCTAG 301 CCGCCGCTCC GGGCTCTGCT CGCCCTCCTA CGTTGCGGTC ACACCCTTCT CCCTTCGGGG 361 AGACAACGAC GGCGGTGGCG GGAGCTTCTC CACGGCCGAC CAGCTGGAGA TGGTGACCGA 421 GCTGCTGGGA GGAGACATGG TGAACCAGAG TTTCATCTGC GACCCGGACG ACGAGACCTT 481 CATCAAAAAC ATCATCATCC AGGACTGTAT GTGGAGCGGC TTCTCGGCCG CCGCCAAGCT 541 CGTCTCAGAG AAGCTGGCCT CCTACCAGGC TGCGCGCAAA GACAGCGGCA GCCCGAACCC 601 CGCCCGCGGC CACAGCGTCT GCTCCACCTC CAGCTTGTAC CTGCAGGATC TGAGCGCCGC 661 CGCCTCAGAG TGCATCGACC CCTCGGTGGT CTTCCCCTAC CCTCTCAACG ACAGCAGCTC 721 GCCCAAGTCC TGCGCCTCGC AAGACTCCAG CGCCTTCTCT CCGTCCTCGG ATTCTCTGCT 781 CTCCTCGACG GAGTCCTCCC CGCAGGGCAG CCCCGAGCCC CTGGTGCTCC ATGAGGAGAC 841 ACCGCCCACC ACCAGCAGCG ACTCTGAGGA GGAACAAGAA GATGAGGAAG AAATCGATGT 901 TGTTTCTGTG GAAAAGAGGC AAGCTCCTGA CAAAAGGTCA GAGTCTGGAT CACCCTTCTG 961 CTGGAGGCCA CAGCAAACCT CCCTCACAGC CCACTGGTCC TCAAGAGGTG CCACGGTCTC 1021 CACACATCAG CACAACTACG CAGCGGCCTC CCTCCACTCG GAAAGGACTA TCCTTGCTGC 1081 CAAGAAGGGT CCAAGTTGGG AACAGTGTTC AGAAGTCCTT GAAAACGATC AGCTACCAAC 1141 CGAAATGCAC CAGCCCCTCA GGGTCCCTTC GGACACTCGA GAGGAAGAAA TTGTTCTCAG 1201 AGGGGGCGCG TATATCACA //
Primer: IRES-R2 Sequence file: RDB12911_A4Jfb.seq
>12911_12911_A4Jf_1_IRES-R2_A09_03.ab1 1 GAGCCCATCA GGCGGGCTTC GGCAGTAACG TTAGGGGGGG GGGAGGGAGA GGGGCGGATC 61 CGCGGCCATC ACCACTTTGT ACAAGAAAGC TGGGTCGGCG CGCCCACCCT TTACGCACAA 121 GAGTTCCGTA GCTGTTCAAG TTTGTGTTTC AACTGTTCTC GTCGTTTCCG CAACAAGTCC 181 TCTTCAGAAA TGAGCTTTTG CTCCTCTGCT TGGACGGACA GGATGTATGC TGTGGCTTTT 241 TTAAGGATAA CTACCTTGGG GGCCTTTTCA TTGTTTTCCA ACTCCGGGAT CTGGTCACGC 301 AGGGCAAAAA AGCTCCGTTT TAGCTCGTTC CTCCTCTGGC GCTCCAAGAC GTTGTGTGTT 361 CGCCTCTTGA CATTCTCCTC GGTGTCCGAG GACCTGGGGC TGGTGCATTT TCGGTTGTTG 421 CTGATCTGTC TCAGGACTCT GACACTGTCC AACTTGACCC TCTTGGCAGC AGGATAGTCC 481 TTCCGAGTGG AGGGAGGCGC TGCGTAGTTG TGCTGATGTG TGGAGACGTG GCACCTCTTG 541 AGGACCAGTG GGCTGTGAGG AGGTTTGCTG TGGCCTCCAG CAGAAGGTGA TCCAGACTCT 601 GACCTTTTGC CAGGAGCCTG CCTCTTTTCC ACAGAAACAA CATCGATTTC TTCCTCATCT 661 TCTTGTTCCT CCTCAGAGTC GCTGCTGGTG GTGGGCGGTG TCTCTCATGG AGCACCAGGG 721 GCTCGGGGCT GCCCTGGCGG GGAGGACTCC GTCGAGGGAG AGCAGAAGAT CCGAGACGAG 781 AAGAAGCGCT GGGAGTTCTT GCGAGGCGCA GACTTGGGGC GAGCTTGCTT GTCGTTGAAG 841 GTAAGGAGAC CATCGGAGGG GTCGATGCAC TTCTGGAGCG GCGGCGCCTC AATTCCTTGC 901 AAGTAACAAG CCTGGAGGTT GAACCAACGC TGTGGCCCCG GGCGGGATCC GCTTGCTGTG 961 TTCTTGGCGC AACTGTAGAG CACCTTCTGG TACAGACTTG GCGGCGAGAC GTTACA //
Primer: IRES-F Sequence file: RDB12911_A4Jfc.seq
>12911_12911_A4Jf_IRES_F_1_G04_19.ab1 1 GCAGCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG 121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC 181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG 241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC 301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG 361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC 421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG 481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC 541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC 601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC 661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC 721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC 781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCAA GGCCTCTCGA 841 GCCTCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAT 901 TTGTGAAAGA TTGACTGGTA TTCTACTATG TGCTCCTTTT ACGCTATGTG GATACGCTGC 961 TTTATGCCTT GTATCATGCT ATGCTCCCGT ATGCTTCATT TCTCTTCTGA TAATTCTTGC 1021 TGCTGTCTCT TTATGAGAGT GTGTCGTGGT CAAGCACGTG CCTGTGGCAC TGGTTGCTGA 1081 CGCACCCACT GTGGCATGCA CACTGTCAGT CTTCGACTCG CATC //
Primer: CMV-Forward Sequence file: RDB12911_A4Jfd.seq
>12911_12911_A4Jf_CMV_Forward_1_E04_13.ab1 1 TGTCACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT 721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA 781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA 841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC 901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG 961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG 1021 GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGCTATTGA GGCGCAACAG CATCTGTGCA 1081 CTCACAGTCT GGGGCAATCA GCAGCTCAGG CAGATCTGAC CTGGTGGAAA GAAATACCCT 1141 TAAAGGAATT CCA //
Primer: BGH_rev Sequence file: RDB12911_A4Jfe.seq
>12911_12911_A4Jf_BGH_rev_1_H04_22.ab1 1 CGTAATACGG GTCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG 61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT 121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT 181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG 241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC 301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA 361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT 421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA 481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG 541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA 601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT 661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG 721 GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC GAAGACGCGG AAGAGGCCGC 781 AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG 841 CAGAAGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG 901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC 961 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG 1021 TCCCGGGAAG GAGCTGACAG GTGGTGGCAA TGCCTCCAAC CAGTGGGGGT GCGTCAGCAA 1081 ACACAGTGCA CCACCACGGC CACGTGTCTG ACAACGGCCA CAACTTCTCA TAAGAGACAG 1141 CCACAAGATT ATCCAGAGAA AATTGAAGGC CATACCGGAA GCCATTAG //
Primer: SV40pro_F Sequence file: RDB12911_A4Jff.seq
>12911_12911_A4Jf_SV40pro_F_1_A05_02.ab1 1 CCCCGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 1021 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGCGCTCTTC CGCTTTCCTC 1081 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCCGTATCAG CTTCACTTCA 1141 AGCCGGTATT ACGCTTATCC CACAGAATCA ATGGGGATAT AACCGCCCAA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836. [link to RRC of NBRP]
user_report	Hayashi, R., Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS One 7 (9): e45435 (2012). PMID 23029008. [link to RRC of NBRP]