Resource data sheet
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CSII-EF-hc-Myc-IRES2-Venus

Lentivirus vector construct of human MYC for generating iPS cells.

Catalog number RDB12911
Resource name CSII-EF-hc-Myc-IRES2-Venus
Clone info. Lentivirus expression vector of human MYC for generating iPS cells.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Human MYC cDNA
Originator Miyoshi, Hiroyuki |
 
Other clones in our bank human MYC (NCBI Gene 4609) | human MYC (NCBI Gene 4609) |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12911 CSII-EF-hc-Myc-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB12911z.seq from Depositor
Electronic file PDF RDB12911.pdf from Depositor

Featured content

References

original Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12911_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 RDB12911_A4Jfa.seq
>12911_12911_A4Jf_pAxEF_F2_1_F04_16.ab1
    1 GATTTCTCTC CTTTTAGGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT CTGCAGCGGC
   61 CATCACAAGT TTGTACAAAA AAGCAGGCTC CGCGGCCGCC CCCTTCACCA TGCCCCTCAA
  121 CGTTAGCTTC ACCAACAGGA ACTATGACCT CGACTACGAC TCGGTGCAGC CGTATTTCTA
  181 CTGCGACGAG GAGGAGAACT TCTACCAGCA GCAGCAGCAG AGCGAGCTGC AGCCCCCGGC
  241 GCCCAGCGAG GATATCTGGA AGAAATTCGA GCTGCTGCCC ACCCCGCCCC TGTCCCCTAG
  301 CCGCCGCTCC GGGCTCTGCT CGCCCTCCTA CGTTGCGGTC ACACCCTTCT CCCTTCGGGG
  361 AGACAACGAC GGCGGTGGCG GGAGCTTCTC CACGGCCGAC CAGCTGGAGA TGGTGACCGA
  421 GCTGCTGGGA GGAGACATGG TGAACCAGAG TTTCATCTGC GACCCGGACG ACGAGACCTT
  481 CATCAAAAAC ATCATCATCC AGGACTGTAT GTGGAGCGGC TTCTCGGCCG CCGCCAAGCT
  541 CGTCTCAGAG AAGCTGGCCT CCTACCAGGC TGCGCGCAAA GACAGCGGCA GCCCGAACCC
  601 CGCCCGCGGC CACAGCGTCT GCTCCACCTC CAGCTTGTAC CTGCAGGATC TGAGCGCCGC
  661 CGCCTCAGAG TGCATCGACC CCTCGGTGGT CTTCCCCTAC CCTCTCAACG ACAGCAGCTC
  721 GCCCAAGTCC TGCGCCTCGC AAGACTCCAG CGCCTTCTCT CCGTCCTCGG ATTCTCTGCT
  781 CTCCTCGACG GAGTCCTCCC CGCAGGGCAG CCCCGAGCCC CTGGTGCTCC ATGAGGAGAC
  841 ACCGCCCACC ACCAGCAGCG ACTCTGAGGA GGAACAAGAA GATGAGGAAG AAATCGATGT
  901 TGTTTCTGTG GAAAAGAGGC AAGCTCCTGA CAAAAGGTCA GAGTCTGGAT CACCCTTCTG
  961 CTGGAGGCCA CAGCAAACCT CCCTCACAGC CCACTGGTCC TCAAGAGGTG CCACGGTCTC
 1021 CACACATCAG CACAACTACG CAGCGGCCTC CCTCCACTCG GAAAGGACTA TCCTTGCTGC
 1081 CAAGAAGGGT CCAAGTTGGG AACAGTGTTC AGAAGTCCTT GAAAACGATC AGCTACCAAC
 1141 CGAAATGCAC CAGCCCCTCA GGGTCCCTTC GGACACTCGA GAGGAAGAAA TTGTTCTCAG
1201 AGGGGGCGCG TATATCACA
//
Primer: IRES-R2 RDB12911_A4Jfb.seq
>12911_12911_A4Jf_1_IRES-R2_A09_03.ab1
    1 GAGCCCATCA GGCGGGCTTC GGCAGTAACG TTAGGGGGGG GGGAGGGAGA GGGGCGGATC
   61 CGCGGCCATC ACCACTTTGT ACAAGAAAGC TGGGTCGGCG CGCCCACCCT TTACGCACAA
  121 GAGTTCCGTA GCTGTTCAAG TTTGTGTTTC AACTGTTCTC GTCGTTTCCG CAACAAGTCC
  181 TCTTCAGAAA TGAGCTTTTG CTCCTCTGCT TGGACGGACA GGATGTATGC TGTGGCTTTT
  241 TTAAGGATAA CTACCTTGGG GGCCTTTTCA TTGTTTTCCA ACTCCGGGAT CTGGTCACGC
  301 AGGGCAAAAA AGCTCCGTTT TAGCTCGTTC CTCCTCTGGC GCTCCAAGAC GTTGTGTGTT
  361 CGCCTCTTGA CATTCTCCTC GGTGTCCGAG GACCTGGGGC TGGTGCATTT TCGGTTGTTG
  421 CTGATCTGTC TCAGGACTCT GACACTGTCC AACTTGACCC TCTTGGCAGC AGGATAGTCC
  481 TTCCGAGTGG AGGGAGGCGC TGCGTAGTTG TGCTGATGTG TGGAGACGTG GCACCTCTTG
  541 AGGACCAGTG GGCTGTGAGG AGGTTTGCTG TGGCCTCCAG CAGAAGGTGA TCCAGACTCT
  601 GACCTTTTGC CAGGAGCCTG CCTCTTTTCC ACAGAAACAA CATCGATTTC TTCCTCATCT
  661 TCTTGTTCCT CCTCAGAGTC GCTGCTGGTG GTGGGCGGTG TCTCTCATGG AGCACCAGGG
  721 GCTCGGGGCT GCCCTGGCGG GGAGGACTCC GTCGAGGGAG AGCAGAAGAT CCGAGACGAG
  781 AAGAAGCGCT GGGAGTTCTT GCGAGGCGCA GACTTGGGGC GAGCTTGCTT GTCGTTGAAG
  841 GTAAGGAGAC CATCGGAGGG GTCGATGCAC TTCTGGAGCG GCGGCGCCTC AATTCCTTGC
  901 AAGTAACAAG CCTGGAGGTT GAACCAACGC TGTGGCCCCG GGCGGGATCC GCTTGCTGTG
961 TTCTTGGCGC AACTGTAGAG CACCTTCTGG TACAGACTTG GCGGCGAGAC GTTACA
//
Primer: IRES-F RDB12911_A4Jfc.seq
>12911_12911_A4Jf_IRES_F_1_G04_19.ab1
    1 GCAGCTTTAT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
  121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
  301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC
  601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC
  721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCAA GGCCTCTCGA
  841 GCCTCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAT
  901 TTGTGAAAGA TTGACTGGTA TTCTACTATG TGCTCCTTTT ACGCTATGTG GATACGCTGC
  961 TTTATGCCTT GTATCATGCT ATGCTCCCGT ATGCTTCATT TCTCTTCTGA TAATTCTTGC
 1021 TGCTGTCTCT TTATGAGAGT GTGTCGTGGT CAAGCACGTG CCTGTGGCAC TGGTTGCTGA
1081 CGCACCCACT GTGGCATGCA CACTGTCAGT CTTCGACTCG CATC
//
Primer: CMV-Forward RDB12911_A4Jfd.seq
>12911_12911_A4Jf_CMV_Forward_1_E04_13.ab1
    1 TGTCACTCCG CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
  841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC
  901 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
  961 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG
 1021 GGTATAGTGC AGCAGCAGAA CAATTTGCTG AGGCTATTGA GGCGCAACAG CATCTGTGCA
 1081 CTCACAGTCT GGGGCAATCA GCAGCTCAGG CAGATCTGAC CTGGTGGAAA GAAATACCCT
1141 TAAAGGAATT CCA
//
Primer: BGH_rev RDB12911_A4Jfe.seq
>12911_12911_A4Jf_BGH_rev_1_H04_22.ab1
    1 CGTAATACGG GTCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
   61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG
  241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC
  301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA
  361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT
  421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA
  481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
  541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
  601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
  661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG
  721 GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC GAAGACGCGG AAGAGGCCGC
  781 AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG
  841 CAGAAGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG
 1021 TCCCGGGAAG GAGCTGACAG GTGGTGGCAA TGCCTCCAAC CAGTGGGGGT GCGTCAGCAA
 1081 ACACAGTGCA CCACCACGGC CACGTGTCTG ACAACGGCCA CAACTTCTCA TAAGAGACAG
1141 CCACAAGATT ATCCAGAGAA AATTGAAGGC CATACCGGAA GCCATTAG
//
Primer: SV40pro_F RDB12911_A4Jff.seq
>12911_12911_A4Jf_SV40pro_F_1_A05_02.ab1
    1 CCCCGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
  961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA
 1021 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGCGCTCTTC CGCTTTCCTC
 1081 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCCGTATCAG CTTCACTTCA
1141 AGCCGGTATT ACGCTTATCC CACAGAATCA ATGGGGATAT AACCGCCCAA G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836.
user report Hayashi, R., Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS One 7 (9): e45435 (2012). PMID 23029008.

2018.10.22

GNP_filter3_RDBDEP_html_181012.pl